{"id":"https://openalex.org/W4390766074","doi":"https://doi.org/10.21203/rs.3.rs-3722259/v1","title":"Evaluating Bacterial Population Changes and Ecological Dynamics in Oil-Impacted Soils Using 16S rRNA Amplicon Sequencing","display_name":"Evaluating Bacterial Population Changes and Ecological Dynamics in Oil-Impacted Soils Using 16S rRNA Amplicon Sequencing","publication_year":2024,"publication_date":"2024-01-12","ids":{"openalex":"https://openalex.org/W4390766074","doi":"https://doi.org/10.21203/rs.3.rs-3722259/v1"},"language":"en","primary_location":{"id":"doi:10.21203/rs.3.rs-3722259/v1","doi":"https://doi.org/10.21203/rs.3.rs-3722259/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-3722259/v1","pdf_url":"https://www.researchsquare.com/article/rs-3722259/latest.pdf","source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"},"volume":null,"type":"preprint","indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.researchsquare.com/article/rs-3722259/latest.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5004194511","display_name":"Teddie Onkabetse Rahube","orcid":"https://orcid.org/0000-0001-5342-7276","observed_orcids":["https://orcid.org/0000-0001-5342-7276"]},"institutions":[{"id":"https://openalex.org/I216286881","display_name":"Botswana International University of Science and Technology","ror":"https://ror.org/04cr2sq58","country_code":"BW","type":"education","lineage":["https://openalex.org/I216286881"]}],"countries":["BW"],"is_corresponding":false,"raw_author_name":"Teddie Onkabetse Rahube","raw_affiliation_strings":["Botswana International University of Science and Technology"],"raw_orcid":"https://orcid.org/0000-0001-5342-7276","affiliations":[{"raw_affiliation_string":"Botswana International University of Science and Technology","institution_ids":["https://openalex.org/I216286881"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5060099981","display_name":"Ramganesh Selvarajan","orcid":"https://orcid.org/0000-0002-7104-3599","observed_orcids":["https://orcid.org/0000-0002-7104-3599"]},"institutions":[{"id":"https://openalex.org/I19820366","display_name":"Chinese Academy of Sciences","ror":"https://ror.org/034t30j35","country_code":"CN","type":"government","lineage":["https://openalex.org/I19820366"]},{"id":"https://openalex.org/I216286881","display_name":"Botswana International University of Science and Technology","ror":"https://ror.org/04cr2sq58","country_code":"BW","type":"education","lineage":["https://openalex.org/I216286881"]}],"countries":["BW","CN"],"is_corresponding":false,"raw_author_name":"Ramganesh Selvarajan","raw_affiliation_strings":["Chinese Academy of Sciences","Botswana International University of Science and Technology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Chinese Academy of Sciences","institution_ids":["https://openalex.org/I19820366"]},{"raw_affiliation_string":"Botswana International University of Science and Technology","institution_ids":["https://openalex.org/I216286881"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5093706749","display_name":"Batendi Nduna","orcid":"https://orcid.org/0000-0002-9739-6317","observed_orcids":["https://orcid.org/0000-0002-9739-6317"]},"institutions":[{"id":"https://openalex.org/I19820366","display_name":"Chinese Academy of Sciences","ror":"https://ror.org/034t30j35","country_code":"CN","type":"government","lineage":["https://openalex.org/I19820366"]},{"id":"https://openalex.org/I216286881","display_name":"Botswana International University of Science and Technology","ror":"https://ror.org/04cr2sq58","country_code":"BW","type":"education","lineage":["https://openalex.org/I216286881"]}],"countries":["BW","CN"],"is_corresponding":false,"raw_author_name":"Batendi Nduna","raw_affiliation_strings":["Botswana International University of Science and Technology","Chinese Academy of Sciences"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Botswana International University of Science and Technology","institution_ids":["https://openalex.org/I216286881"]},{"raw_affiliation_string":"Chinese Academy of Sciences","institution_ids":["https://openalex.org/I19820366"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5040472136","display_name":"Bokani Nthaba","orcid":"https://orcid.org/0000-0002-0341-049X","observed_orcids":["https://orcid.org/0000-0002-0341-049X"]},"institutions":[{"id":"https://openalex.org/I216286881","display_name":"Botswana International University of Science and Technology","ror":"https://ror.org/04cr2sq58","country_code":"BW","type":"education","lineage":["https://openalex.org/I216286881"]}],"countries":["BW"],"is_corresponding":false,"raw_author_name":"Bokani Nthaba","raw_affiliation_strings":["Botswana International University of Science and Technology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Botswana International University of Science and Technology","institution_ids":["https://openalex.org/I216286881"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5030582408","display_name":"Loago N. Molwalefhe","orcid":"https://orcid.org/0000-0001-9929-2223","observed_orcids":["https://orcid.org/0000-0001-9929-2223"]},"institutions":[{"id":"https://openalex.org/I216286881","display_name":"Botswana International University of Science and Technology","ror":"https://ror.org/04cr2sq58","country_code":"BW","type":"education","lineage":["https://openalex.org/I216286881"]}],"countries":["BW"],"is_corresponding":false,"raw_author_name":"Loago Molwalefhe","raw_affiliation_strings":["Botswana International University of Science and Technology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Botswana International University of Science and Technology","institution_ids":["https://openalex.org/I216286881"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5008739107","display_name":"Elisha Shemang","orcid":"https://orcid.org/0000-0003-2865-4148","observed_orcids":["https://orcid.org/0000-0003-2865-4148"]},"institutions":[{"id":"https://openalex.org/I216286881","display_name":"Botswana International University of Science and Technology","ror":"https://ror.org/04cr2sq58","country_code":"BW","type":"education","lineage":["https://openalex.org/I216286881"]}],"countries":["BW"],"is_corresponding":false,"raw_author_name":"Elisha Shemang","raw_affiliation_strings":["Botswana International University of Science and Technology","Botswana International University of Science and Technology Bokani Nthaba Botswana International University of Science and Technology Loago Molwalefhe Botswana International University of Science and Technology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Botswana International University of Science and Technology","institution_ids":["https://openalex.org/I216286881"]},{"raw_affiliation_string":"Botswana International University of Science and Technology Bokani Nthaba Botswana International University of Science and Technology Loago Molwalefhe Botswana International University of Science and Technology","institution_ids":["https://openalex.org/I216286881"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10324","display_name":"Microbial bioremediation and biosurfactants","score":0.9970999956130981,"subfield":{"id":"https://openalex.org/subfields/2310","display_name":"Pollution"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10324","display_name":"Microbial bioremediation and biosurfactants","score":0.9970999956130981,"subfield":{"id":"https://openalex.org/subfields/2310","display_name":"Pollution"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11791","display_name":"Microbial Community Ecology and Physiology","score":0.002899999963119626,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12180","display_name":"Polar Research and Ecology","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/used-engine-oil","display_name":"used motor oil","score":0.8180000185966492},{"id":"https://openalex.org/keywords/soil-contamination","display_name":"soil contamination","score":0.7429999709129333},{"id":"https://openalex.org/keywords/bacterial-community-composition","display_name":"bacterial community composition","score":0.7179999947547913},{"id":"https://openalex.org/keywords/gram-positive-bacteria","display_name":"Gram-positive bacteria","score":0.6629999876022339},{"id":"https://openalex.org/keywords/gram-negative-bacteria","display_name":"Gram-negative bacteria","score":0.8700000047683716},{"id":"https://openalex.org/keywords/polycyclic-aromatic-hydrocarbons","display_name":"polycyclic aromatic hydrocarbons","score":0.9309999942779541},{"id":"https://openalex.org/keywords/biodegradation","display_name":"biodegradation","score":0.8820000290870667},{"id":"https://openalex.org/keywords/soil-bacteria","display_name":"soil bacteria","score":0.3880000114440918},{"id":"https://openalex.org/keywords/methylobacterium","display_name":"Methylobacterium","score":0.7730000019073486},{"id":"https://openalex.org/keywords/bradyrhizobium","display_name":"Bradyrhizobium","score":0.9990000128746033},{"id":"https://openalex.org/keywords/phyllobacterium","display_name":"Phyllobacterium","score":0.9900000095367432},{"id":"https://openalex.org/keywords/plant-associated-bacteria","display_name":"plant-associated bacteria","score":0.6620000004768372},{"id":"https://openalex.org/keywords/soil-remediation","display_name":"soil remediation","score":0.6349999904632568},{"id":"https://openalex.org/keywords/botswana","display_name":"Botswana","score":0.8270000219345093}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.627241313457489},{"id":"https://openalex.org/C15151743","wikidata":"https://www.wikidata.org/wiki/Q903778","display_name":"Metagenomics","level":3,"score":0.6014291644096375},{"id":"https://openalex.org/C2993206177","wikidata":"https://www.wikidata.org/wiki/Q2844457","display_name":"Amplicon sequencing","level":4,"score":0.5468343496322632},{"id":"https://openalex.org/C2908647359","wikidata":"https://www.wikidata.org/wiki/Q2625603","display_name":"Population","level":2,"score":0.5245516300201416},{"id":"https://openalex.org/C2779676784","wikidata":"https://www.wikidata.org/wiki/Q130999","display_name":"Proteobacteria","level":4,"score":0.5140315294265747},{"id":"https://openalex.org/C81407943","wikidata":"https://www.wikidata.org/wiki/Q17149178","display_name":"Microbial population biology","level":3,"score":0.5018811225891113},{"id":"https://openalex.org/C42062724","wikidata":"https://www.wikidata.org/wiki/Q1209205","display_name":"16S ribosomal RNA","level":3,"score":0.4648773670196533},{"id":"https://openalex.org/C112570922","wikidata":"https://www.wikidata.org/wiki/Q60528603","display_name":"Contamination","level":2,"score":0.4436550438404083},{"id":"https://openalex.org/C122325731","wikidata":"https://www.wikidata.org/wiki/Q3738459","display_name":"Relative species abundance","level":3,"score":0.4329514801502228},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.4273895025253296},{"id":"https://openalex.org/C65580899","wikidata":"https://www.wikidata.org/wiki/Q212734","display_name":"Soil contamination","level":3,"score":0.42464590072631836},{"id":"https://openalex.org/C110872660","wikidata":"https://www.wikidata.org/wiki/Q37813","display_name":"Ecosystem","level":2,"score":0.423002690076828},{"id":"https://openalex.org/C77077793","wikidata":"https://www.wikidata.org/wiki/Q336019","display_name":"Abundance (ecology)","level":2,"score":0.38120001554489136},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.29508885741233826},{"id":"https://openalex.org/C144024400","wikidata":"https://www.wikidata.org/wiki/Q21201","display_name":"Sociology","level":0,"score":0.0},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0},{"id":"https://openalex.org/C149923435","wikidata":"https://www.wikidata.org/wiki/Q37732","display_name":"Demography","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.21203/rs.3.rs-3722259/v1","doi":"https://doi.org/10.21203/rs.3.rs-3722259/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-3722259/v1","pdf_url":"https://www.researchsquare.com/article/rs-3722259/latest.pdf","source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.21203/rs.3.rs-3722259/v1","doi":"https://doi.org/10.21203/rs.3.rs-3722259/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-3722259/v1","pdf_url":"https://www.researchsquare.com/article/rs-3722259/latest.pdf","source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/15","display_name":"Life on land","score":0.9599999785423279}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320311058","display_name":"Botswana International University of Science and Technology","ror":"https://ror.org/04cr2sq58"},{"id":"https://openalex.org/F4320334105","display_name":"Center for High Performance Computing","ror":"https://ror.org/021f4v087"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4390766074.pdf","grobid_xml":"https://content.openalex.org/works/W4390766074.grobid-xml"},"referenced_works_count":38,"referenced_works":["https://openalex.org/W1881487528","https://openalex.org/W2014438651","https://openalex.org/W2025523427","https://openalex.org/W2075364748","https://openalex.org/W2093290747","https://openalex.org/W2162537107","https://openalex.org/W2201735116","https://openalex.org/W2466025386","https://openalex.org/W2607762545","https://openalex.org/W2957852930","https://openalex.org/W2978355872","https://openalex.org/W2985081606","https://openalex.org/W2990030177","https://openalex.org/W2999898057","https://openalex.org/W3000159728","https://openalex.org/W3001859424","https://openalex.org/W3002007664","https://openalex.org/W3003280388","https://openalex.org/W3042062970","https://openalex.org/W3045315617","https://openalex.org/W3129100435","https://openalex.org/W3142428231","https://openalex.org/W3155720236","https://openalex.org/W3163676806","https://openalex.org/W3188232888","https://openalex.org/W3204688550","https://openalex.org/W3210555841","https://openalex.org/W4200111264","https://openalex.org/W4220677553","https://openalex.org/W4235702403","https://openalex.org/W4288802256","https://openalex.org/W4292162558","https://openalex.org/W4292608283","https://openalex.org/W4307635381","https://openalex.org/W4313479287","https://openalex.org/W4321101506","https://openalex.org/W4378974421","https://openalex.org/W4381943588"],"related_works":["https://openalex.org/W7097579398","https://openalex.org/W2161046928","https://openalex.org/W2791639362","https://openalex.org/W3083907272","https://openalex.org/W2898944376","https://openalex.org/W2191134575","https://openalex.org/W2576060123","https://openalex.org/W4413286382","https://openalex.org/W2999366018","https://openalex.org/W2595950379"],"abstract_inverted_index":{"Abstract":[0],"Improper":[1],"disposal":[2],"of":[3,55,139,169,180,183,198],"used":[4,56,75,116],"motor":[5,57,76,117],"oil":[6,45,58,77,146,170],"is":[7,59],"a":[8,16],"prevalent":[9],"issue":[10],"in":[11,37,49,161,190,202],"developing":[12],"countries,":[13],"leading":[14],"to":[15,19,33,80,107,137],"notable":[17,103],"contribution":[18],"environmental":[20],"pollution.":[21],"This":[22],"study":[23],"was":[24,111],"conducted":[25],"using":[26],"the":[27,35,53,61,98,124,128,140,166,181,195],"16S":[28],"rRNA":[29],"targeted":[30],"metagenomic":[31],"approach,":[32],"assess":[34],"changes":[36],"bacterial":[38,90,109,153],"population":[39],"diversity":[40],"and":[41,71,85,100,120,149,187,207],"abundance":[42],"at":[43,67],"an":[44],"contamination":[46],"experimental":[47],"site":[48],"Botswana.":[50],"To":[51],"demonstrate":[52],"impact":[54,168],"on":[60],"soil":[62,64,185],"ecosystem,":[63],"samples":[65],"collected":[66],"different":[68],"depths":[69],"before":[70],"after":[72],"treatment":[73,114],"with":[74,115,145,204],"were":[78,122],"subjected":[79],"total":[81],"community":[82],"DNA":[83],"extraction":[84],"Illumina":[86],"sequencing.":[87],"The":[88,172],"taxonomic":[89],"composition":[91],"data":[92],"revealed":[93],"statistically":[94],"significant":[95],"differences":[96],"among":[97,123],"treatments":[99],"controls.":[101],"A":[102],"shift":[104],"from":[105,174],"Gram-negative":[106],"Gram-positive":[108,130],"populations":[110],"observed":[112],"following":[113],"oil.":[118],"Prevotella":[119],"Aerococcus":[121],"few":[125],"genera":[126],"within":[127],"enriched":[129],"bacteria":[131],"that":[132],"could":[133],"be":[134],"directly":[135],"linked":[136],"biodegradation":[138],"polycyclic":[141],"aromatic":[142],"hydrocarbons":[143],"associated":[144],"contamination.":[147,171],"Agricultural":[148],"biotechnologically":[150],"important,":[151],"plant-associated":[152],"genera;":[154],"Methylobacterium-methylorumbrum,":[155],"Bradyrhizobium,":[156],"and,":[157],"Phyllobacterium":[158],"significantly":[159],"declined":[160],"relative":[162],"abundance,":[163],"thus":[164],"demonstrating":[165],"negative":[167],"results":[173],"this":[175],"study,":[176],"improves":[177],"our":[178],"understanding":[179],"roles":[182],"indigenous":[184],"bacteria,":[186],"can":[188],"help":[189],"guiding":[191],"future":[192],"strategies":[193],"for":[194],"sustainable":[196],"management":[197],"contaminated":[199],"soils":[200],"globally":[201],"countries":[203],"similar":[205],"climatic":[206],"ecological":[208],"conditions.":[209]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2024-01-13T00:00:00"}