{"id":"https://openalex.org/W4390739223","doi":"https://doi.org/10.1021/acs.biochem.3c00509","title":"Thermal Stabilization of a Bacterial Zn(II)-Dependent Phospholipase C through Consensus Sequence Design","display_name":"Thermal Stabilization of a Bacterial Zn(II)-Dependent Phospholipase C through Consensus Sequence Design","publication_year":2024,"publication_date":"2024-01-11","ids":{"openalex":"https://openalex.org/W4390739223","doi":"https://doi.org/10.1021/acs.biochem.3c00509","pmid":"https://pubmed.ncbi.nlm.nih.gov/38206322"},"language":"en","primary_location":{"id":"doi:10.1021/acs.biochem.3c00509","doi":"https://doi.org/10.1021/acs.biochem.3c00509","is_oa":false,"landing_page_url":"https://doi.org/10.1021/acs.biochem.3c00509","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2022/10/06/2022.10.06.511049.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5075366621","display_name":"Diego S. Val","orcid":"https://orcid.org/0000-0002-2578-2315","observed_orcids":["https://orcid.org/0000-0002-2578-2315"]},"institutions":[{"id":"https://openalex.org/I151201029","display_name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","ror":"https://ror.org/03cqe8w59","country_code":"AR","type":"government","lineage":["https://openalex.org/I151201029","https://openalex.org/I4210123736","https://openalex.org/I4387155568"]},{"id":"https://openalex.org/I4141589","display_name":"National University of Rosario","ror":"https://ror.org/02tphfq59","country_code":"AR","type":"education","lineage":["https://openalex.org/I4141589"]}],"countries":["AR"],"is_corresponding":false,"raw_author_name":"Diego S. Val","raw_affiliation_strings":["Instituto de Procesos Biotecnol\u00f3gicos y Qu\u00edmicos (IPROBYQ), FbioyF-UNR-CONICET. Mitre 1998, 2000 Rosario, Argentina"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Instituto de Procesos Biotecnol\u00f3gicos y Qu\u00edmicos (IPROBYQ), FbioyF-UNR-CONICET. Mitre 1998, 2000 Rosario, Argentina","institution_ids":["https://openalex.org/I151201029","https://openalex.org/I4141589"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5073897950","display_name":"Luisina Di Nardo","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I151201029","display_name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","ror":"https://ror.org/03cqe8w59","country_code":"AR","type":"government","lineage":["https://openalex.org/I151201029","https://openalex.org/I4210123736","https://openalex.org/I4387155568"]},{"id":"https://openalex.org/I4141589","display_name":"National University of Rosario","ror":"https://ror.org/02tphfq59","country_code":"AR","type":"education","lineage":["https://openalex.org/I4141589"]},{"id":"https://openalex.org/I4210147873","display_name":"Instituto de Biolog\u00eda Molecular y Celular de Rosario","ror":"https://ror.org/04x0n3178","country_code":"AR","type":"facility","lineage":["https://openalex.org/I151201029","https://openalex.org/I4141589","https://openalex.org/I4210107757","https://openalex.org/I4210123736","https://openalex.org/I4210147873","https://openalex.org/I4387155568"]}],"countries":["AR"],"is_corresponding":false,"raw_author_name":"Luisina Di Nardo","raw_affiliation_strings":["Instituto de Biolog\u00eda Celular y Molecular de Rosario (IBR), FbioyF-UNR-CONICET. Ocampo y Esmeralda, 2000 Rosario, Argentina"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Instituto de Biolog\u00eda Celular y Molecular de Rosario (IBR), FbioyF-UNR-CONICET. Ocampo y Esmeralda, 2000 Rosario, Argentina","institution_ids":["https://openalex.org/I151201029","https://openalex.org/I4141589","https://openalex.org/I4210147873"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5018187057","display_name":"Fiorela Marchisio","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I151201029","display_name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","ror":"https://ror.org/03cqe8w59","country_code":"AR","type":"government","lineage":["https://openalex.org/I151201029","https://openalex.org/I4210123736","https://openalex.org/I4387155568"]},{"id":"https://openalex.org/I4141589","display_name":"National University of Rosario","ror":"https://ror.org/02tphfq59","country_code":"AR","type":"education","lineage":["https://openalex.org/I4141589"]}],"countries":["AR"],"is_corresponding":false,"raw_author_name":"Fiorela Marchisio","raw_affiliation_strings":["Instituto de Procesos Biotecnol\u00f3gicos y Qu\u00edmicos (IPROBYQ), FbioyF-UNR-CONICET. Mitre 1998, 2000 Rosario, Argentina"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Instituto de Procesos Biotecnol\u00f3gicos y Qu\u00edmicos (IPROBYQ), FbioyF-UNR-CONICET. Mitre 1998, 2000 Rosario, Argentina","institution_ids":["https://openalex.org/I151201029","https://openalex.org/I4141589"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5026105193","display_name":"Salvador Peir\u00fa","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Salvador Peiru","raw_affiliation_strings":["Keclon S.A., Cerrito 847, 2000 Rosario, Argentina"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Keclon S.A., Cerrito 847, 2000 Rosario, Argentina","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5081880815","display_name":"Mar\u00eda Eugenia Castelli","orcid":"https://orcid.org/0000-0002-9449-9177","observed_orcids":["https://orcid.org/0000-0002-9449-9177"]},"institutions":[{"id":"https://openalex.org/I151201029","display_name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","ror":"https://ror.org/03cqe8w59","country_code":"AR","type":"government","lineage":["https://openalex.org/I151201029","https://openalex.org/I4210123736","https://openalex.org/I4387155568"]},{"id":"https://openalex.org/I4141589","display_name":"National University of Rosario","ror":"https://ror.org/02tphfq59","country_code":"AR","type":"education","lineage":["https://openalex.org/I4141589"]}],"countries":["AR"],"is_corresponding":false,"raw_author_name":"Mar\u00eda Eugenia Castelli","raw_affiliation_strings":["Instituto de Procesos Biotecnol\u00f3gicos y Qu\u00edmicos (IPROBYQ), FbioyF-UNR-CONICET. Mitre 1998, 2000 Rosario, Argentina"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Instituto de Procesos Biotecnol\u00f3gicos y Qu\u00edmicos (IPROBYQ), FbioyF-UNR-CONICET. Mitre 1998, 2000 Rosario, Argentina","institution_ids":["https://openalex.org/I151201029","https://openalex.org/I4141589"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5016180753","display_name":"Luciano A. Abriata","orcid":"https://orcid.org/0000-0003-3087-8677","observed_orcids":["https://orcid.org/0000-0003-3087-8677"]},"institutions":[{"id":"https://openalex.org/I5124864","display_name":"\u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne","ror":"https://ror.org/02s376052","country_code":"CH","type":"education","lineage":["https://openalex.org/I2799323385","https://openalex.org/I5124864"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Luciano Andr\u00e9s Abriata","raw_affiliation_strings":["Ecole Polytechnique F\u00e9d\u00e9rale de Lausanne, CH-1015 Lausanne, Switzerland"],"raw_orcid":"https://orcid.org/0000-0003-3087-8677","affiliations":[{"raw_affiliation_string":"Ecole Polytechnique F\u00e9d\u00e9rale de Lausanne, CH-1015 Lausanne, Switzerland","institution_ids":["https://openalex.org/I5124864"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5020401119","display_name":"Hugo G. Menzella","orcid":"https://orcid.org/0000-0001-6930-2400","observed_orcids":["https://orcid.org/0000-0001-6930-2400"]},"institutions":[{"id":"https://openalex.org/I151201029","display_name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","ror":"https://ror.org/03cqe8w59","country_code":"AR","type":"government","lineage":["https://openalex.org/I151201029","https://openalex.org/I4210123736","https://openalex.org/I4387155568"]},{"id":"https://openalex.org/I4141589","display_name":"National University of Rosario","ror":"https://ror.org/02tphfq59","country_code":"AR","type":"education","lineage":["https://openalex.org/I4141589"]}],"countries":["AR"],"is_corresponding":false,"raw_author_name":"Hugo G. Menzella","raw_affiliation_strings":["Instituto de Procesos Biotecnol\u00f3gicos y Qu\u00edmicos (IPROBYQ), FbioyF-UNR-CONICET. Mitre 1998, 2000 Rosario, Argentina"],"raw_orcid":"https://orcid.org/0000-0001-6930-2400","affiliations":[{"raw_affiliation_string":"Instituto de Procesos Biotecnol\u00f3gicos y Qu\u00edmicos (IPROBYQ), FbioyF-UNR-CONICET. Mitre 1998, 2000 Rosario, Argentina","institution_ids":["https://openalex.org/I151201029","https://openalex.org/I4141589"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5025421454","display_name":"Rodolfo Maximiliano Rasia","orcid":"https://orcid.org/0000-0003-3940-067X","observed_orcids":["https://orcid.org/0000-0003-3940-067X"]},"institutions":[{"id":"https://openalex.org/I151201029","display_name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","ror":"https://ror.org/03cqe8w59","country_code":"AR","type":"government","lineage":["https://openalex.org/I151201029","https://openalex.org/I4210123736","https://openalex.org/I4387155568"]},{"id":"https://openalex.org/I4141589","display_name":"National University of Rosario","ror":"https://ror.org/02tphfq59","country_code":"AR","type":"education","lineage":["https://openalex.org/I4141589"]},{"id":"https://openalex.org/I4210147873","display_name":"Instituto de Biolog\u00eda Molecular y Celular de Rosario","ror":"https://ror.org/04x0n3178","country_code":"AR","type":"facility","lineage":["https://openalex.org/I151201029","https://openalex.org/I4141589","https://openalex.org/I4210107757","https://openalex.org/I4210123736","https://openalex.org/I4210147873","https://openalex.org/I4387155568"]}],"countries":["AR"],"is_corresponding":true,"raw_author_name":"Rodolfo M. Rasia","raw_affiliation_strings":["Instituto de Biolog\u00eda Celular y Molecular de Rosario (IBR), FbioyF-UNR-CONICET. Ocampo y Esmeralda, 2000 Rosario, Argentina","Plataforma Argentina de Biolog\u00eda Estructural y Metabol\u00f3mica, Ocampo y Esmeralda, 2000 Rosario, Argentina"],"raw_orcid":"https://orcid.org/0000-0003-3940-067X","affiliations":[{"raw_affiliation_string":"Instituto de Biolog\u00eda Celular y Molecular de Rosario (IBR), FbioyF-UNR-CONICET. Ocampo y Esmeralda, 2000 Rosario, Argentina","institution_ids":["https://openalex.org/I151201029","https://openalex.org/I4141589","https://openalex.org/I4210147873"]},{"raw_affiliation_string":"Plataforma Argentina de Biolog\u00eda Estructural y Metabol\u00f3mica, Ocampo y Esmeralda, 2000 Rosario, Argentina","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5025421454"],"corresponding_institution_ids":["https://openalex.org/I151201029","https://openalex.org/I4141589","https://openalex.org/I4210147873"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":0.3453,"has_fulltext":false,"cited_by_count":4,"citation_normalized_percentile":{"value":0.44680024,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":95},"biblio":{"volume":"63","issue":"3","first_page":"348","last_page":"354"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.7343999743461609,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.7343999743461609,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.1395999938249588,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.08590000122785568,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/thermal-stabilization","display_name":"thermal stabilization","score":0.7680000066757202},{"id":"https://openalex.org/keywords/phospholipase-c","display_name":"phospholipase C","score":0.8259999752044678},{"id":"https://openalex.org/keywords/protein-engineering","display_name":"protein engineering","score":0.7929999828338623},{"id":"https://openalex.org/keywords/protein-stability","display_name":"protein stability","score":0.7570000290870667},{"id":"https://openalex.org/keywords/protein-unfolding","display_name":"protein unfolding","score":0.781000018119812},{"id":"https://openalex.org/keywords/industrial-enzymes","display_name":"industrial enzymes","score":0.7490000128746033},{"id":"https://openalex.org/keywords/bacterial-enzymes","display_name":"bacterial enzymes","score":0.5759999752044678}],"concepts":[{"id":"https://openalex.org/C54985914","wikidata":"https://www.wikidata.org/wiki/Q1295754","display_name":"Consensus sequence","level":4,"score":0.6374855637550354},{"id":"https://openalex.org/C147816474","wikidata":"https://www.wikidata.org/wiki/Q169525","display_name":"Protein engineering","level":3,"score":0.6050335764884949},{"id":"https://openalex.org/C2778112365","wikidata":"https://www.wikidata.org/wiki/Q3511065","display_name":"Sequence (biology)","level":2,"score":0.5783733129501343},{"id":"https://openalex.org/C2988375501","wikidata":"https://www.wikidata.org/wiki/Q847556","display_name":"Protein stability","level":2,"score":0.5667212009429932},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.5552072525024414},{"id":"https://openalex.org/C59061564","wikidata":"https://www.wikidata.org/wiki/Q7783071","display_name":"Thermal stability","level":2,"score":0.5258815288543701},{"id":"https://openalex.org/C9418097","wikidata":"https://www.wikidata.org/wiki/Q440681","display_name":"Directed evolution","level":4,"score":0.5225992798805237},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5074216723442078},{"id":"https://openalex.org/C2781216036","wikidata":"https://www.wikidata.org/wiki/Q902494","display_name":"Thermostability","level":3,"score":0.4427555501461029},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.41124826669692993},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.38753437995910645},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3694343864917755},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.35809019207954407},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.3171232342720032},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.17042896151542664},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D015032","descriptor_name":"Zinc","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D016384","descriptor_name":"Consensus Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":5,"locations":[{"id":"doi:10.1021/acs.biochem.3c00509","doi":"https://doi.org/10.1021/acs.biochem.3c00509","is_oa":false,"landing_page_url":"https://doi.org/10.1021/acs.biochem.3c00509","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},{"id":"doi:10.1101/2022.10.06.511049","doi":"https://doi.org/10.1101/2022.10.06.511049","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2022.10.06.511049","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2022/10/06/2022.10.06.511049.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},{"id":"pmid:38206322","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/38206322","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Journal Article"},{"id":"pmh:oai:infoscience.epfl.ch:309108","doi":null,"is_oa":true,"landing_page_url":"https://infoscience.epfl.ch/handle/20.500.14299/205529","pdf_url":null,"source":{"id":"https://openalex.org/S4306400487","display_name":"Infoscience (Ecole Polytechnique F\u00e9d\u00e9rale de Lausanne)","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I5124864","host_organization_name":"\u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne","host_organization_lineage":["https://openalex.org/I5124864"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"WoS","raw_type":"research article"},{"id":"pmh:oai:ri.conicet.gov.ar:11336/262576","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/11336/262576","pdf_url":null,"source":{"id":"https://openalex.org/S4306401400","display_name":"Conicet","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210104196","host_organization_name":"Centro Cient\u00edfico Tecnol\u00f3gico - Nordeste","host_organization_lineage":["https://openalex.org/I4210104196"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":{"id":"doi:10.1101/2022.10.06.511049","doi":"https://doi.org/10.1101/2022.10.06.511049","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2022.10.06.511049","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2022/10/06/2022.10.06.511049.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G109880295","display_name":null,"funder_award_id":"PICT2016-4124","funder_id":"https://openalex.org/F4320337954","funder_display_name":"Fondo para la Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica"},{"id":"https://openalex.org/G1505758168","display_name":null,"funder_award_id":"IO 1027-00332","funder_id":"https://openalex.org/F4320329199","funder_display_name":"Agencia Santafesina de Ciencia, Tecnolog\u00eda e Innovaci\u00f3n"},{"id":"https://openalex.org/G3502230702","display_name":null,"funder_award_id":"PICT-2019-01528","funder_id":"https://openalex.org/F4320337954","funder_display_name":"Fondo para la Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica"}],"funders":[{"id":"https://openalex.org/F4320321594","display_name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","ror":"https://ror.org/03cqe8w59"},{"id":"https://openalex.org/F4320329199","display_name":"Agencia Santafesina de Ciencia, Tecnolog\u00eda e Innovaci\u00f3n","ror":"https://ror.org/05e65a079"},{"id":"https://openalex.org/F4320337954","display_name":"Fondo para la Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","ror":"https://ror.org/03stxzb56"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4390739223.pdf","grobid_xml":"https://content.openalex.org/works/W4390739223.grobid-xml"},"referenced_works_count":30,"referenced_works":["https://openalex.org/W1513717105","https://openalex.org/W1652794929","https://openalex.org/W1868359274","https://openalex.org/W1965719743","https://openalex.org/W1977110200","https://openalex.org/W2048765448","https://openalex.org/W2052708596","https://openalex.org/W2107867854","https://openalex.org/W2125135723","https://openalex.org/W2138838827","https://openalex.org/W2412714128","https://openalex.org/W2560264526","https://openalex.org/W2579261433","https://openalex.org/W2766237759","https://openalex.org/W2893348872","https://openalex.org/W2938803199","https://openalex.org/W2947052136","https://openalex.org/W2952323901","https://openalex.org/W2995581132","https://openalex.org/W3021535199","https://openalex.org/W3042348466","https://openalex.org/W3170241704","https://openalex.org/W3177828909","https://openalex.org/W3186179742","https://openalex.org/W4230046749","https://openalex.org/W4235299590","https://openalex.org/W4285891261","https://openalex.org/W4296032638","https://openalex.org/W4360815594","https://openalex.org/W4390778100"],"related_works":["https://openalex.org/W4251665338","https://openalex.org/W2952323901","https://openalex.org/W1983090887","https://openalex.org/W1515330096","https://openalex.org/W4402609760","https://openalex.org/W3042348466","https://openalex.org/W2060194574","https://openalex.org/W2952571660","https://openalex.org/W4410410565","https://openalex.org/W2117515539"],"abstract_inverted_index":{"Proteins'":[0],"extraordinary":[1],"performance":[2],"in":[3,12,30,43,54,68,152,176],"recognition":[4],"and":[5,91,105,115,129],"catalysis":[6],"has":[7],"led":[8],"to":[9,38,51,138,144],"their":[10],"use":[11,29],"a":[13,41,75,80,99],"range":[14],"of":[15,60,63,79,101,121,125,159,169],"applications.":[16,186],"However,":[17],"proteins":[18,64],"obtained":[19],"from":[20,95],"natural":[21,131],"sources":[22],"are":[23],"oftentimes":[24],"not":[25],"suitable":[26,183],"for":[27,103,147,184],"direct":[28],"industrial":[31,185],"or":[32],"diagnostic":[33],"setups.":[34],"Natural":[35],"proteins,":[36],"evolved":[37],"optimally":[39],"perform":[40],"task":[42],"physiological":[44],"conditions,":[45,180],"usually":[46],"lack":[47],"the":[48,58,61,112,122,140,148,156,160,166,170],"stability":[49,62,114,123],"required":[50],"be":[52,145],"used":[53],"harsher":[55],"conditions.":[56],"Therefore,":[57],"alteration":[59],"is":[65],"commonly":[66],"pursued":[67],"protein":[69,171],"engineering":[70],"studies.":[71],"Here,":[72],"we":[73],"achieved":[74],"substantial":[76],"thermal":[77],"stabilization":[78],"bacterial":[81],"Zn(II)-dependent":[82],"phospholipase":[83],"C":[84],"by":[85],"consensus":[86,109,161],"sequence":[87,110],"design.":[88],"We":[89,163],"retrieved":[90],"analyzed":[92],"sequenced":[93],"homologues":[94],"different":[96],"sources,":[97],"selecting":[98],"subset":[100],"examples":[102],"expression":[104],"characterization.":[106],"A":[107],"non-natural":[108],"showed":[111],"highest":[113],"activity":[116,174],"among":[117],"those":[118],"tested.":[119],"Comparison":[120],"parameters":[124],"this":[126],"stabilized":[127,167],"mutant":[128],"other":[130],"variants":[132],"bearing":[133],"similar":[134],"mutations":[135,151],"allows":[136],"us":[137],"pinpoint":[139],"sites":[141,154],"most":[142],"likely":[143],"responsible":[146],"enhancement.":[149],"Point":[150],"these":[153],"alter":[155],"unfolding":[157],"process":[158],"sequence.":[162],"show":[164],"that":[165],"version":[168],"retains":[172],"full":[173],"even":[175],"harsh":[177],"oil":[178],"degumming":[179],"making":[181],"it":[182]},"counts_by_year":[{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1}],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2024-01-13T00:00:00"}