{"id":"https://openalex.org/W4389026524","doi":"https://doi.org/10.1096/fasebj.30.1_supplement.856.5","title":"Single Molecule Analysis of Allosteric Interactions in Protein Kinase A","display_name":"Single Molecule Analysis of Allosteric Interactions in Protein Kinase A","publication_year":2016,"publication_date":"2016-04-01","ids":{"openalex":"https://openalex.org/W4389026524","doi":"https://doi.org/10.1096/fasebj.30.1_supplement.856.5"},"language":"en","primary_location":{"id":"doi:10.1096/fasebj.30.1_supplement.856.5","doi":"https://doi.org/10.1096/fasebj.30.1_supplement.856.5","is_oa":false,"landing_page_url":"https://doi.org/10.1096/fasebj.30.1_supplement.856.5","pdf_url":null,"source":{"id":"https://openalex.org/S25293849","display_name":"The FASEB Journal","issn_l":"0892-6638","issn":["0892-6638","1530-6860"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FASEB Journal","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101800686","display_name":"Yuxin Hao","orcid":"https://orcid.org/0000-0003-1155-3367","observed_orcids":["https://orcid.org/0000-0003-1155-3367"]},"institutions":[{"id":"https://openalex.org/I184565670","display_name":"Georgetown University","ror":"https://ror.org/05vzafd60","country_code":"US","type":"education","lineage":["https://openalex.org/I184565670"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yuxin Hao","raw_affiliation_strings":["Department of Chemistry, Georgetown University, Washington, DC"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Georgetown University, Washington, DC","institution_ids":["https://openalex.org/I184565670"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5070445127","display_name":"Jeneffer P. England","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I184565670","display_name":"Georgetown University","ror":"https://ror.org/05vzafd60","country_code":"US","type":"education","lineage":["https://openalex.org/I184565670"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jeneffer England","raw_affiliation_strings":["Department of Chemistry, Georgetown University, Washington, DC"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Georgetown University, Washington, DC","institution_ids":["https://openalex.org/I184565670"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5045962448","display_name":"Susan Serota Taylor","orcid":"https://orcid.org/0000-0002-7702-6108","observed_orcids":["https://orcid.org/0000-0002-7702-6108"]},"institutions":[{"id":"https://openalex.org/I36258959","display_name":"University of California San Diego","ror":"https://ror.org/0168r3w48","country_code":"US","type":"education","lineage":["https://openalex.org/I36258959"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Susan S. Taylor","raw_affiliation_strings":["Department of Pharmacology, University of California, San Diego, La Jolla, CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmacology, University of California, San Diego, La Jolla, CA","institution_ids":["https://openalex.org/I36258959"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5031847066","display_name":"Rodrigo A. Maillard","orcid":"https://orcid.org/0000-0001-5078-6775","observed_orcids":["https://orcid.org/0000-0001-5078-6775"]},"institutions":[{"id":"https://openalex.org/I184565670","display_name":"Georgetown University","ror":"https://ror.org/05vzafd60","country_code":"US","type":"education","lineage":["https://openalex.org/I184565670"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Rodrigo A Maillard","raw_affiliation_strings":["Department of Chemistry, Georgetown University, Washington, DC"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Georgetown University, Washington, DC","institution_ids":["https://openalex.org/I184565670"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3740,"currency":"USD","value_usd":3740},"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.27478001,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"30","issue":"S1","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.5034000277519226,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.5034000277519226,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10923","display_name":"Force Microscopy Techniques and Applications","score":0.4657999873161316,"subfield":{"id":"https://openalex.org/subfields/3107","display_name":"Atomic and Molecular Physics, and Optics"},"field":{"id":"https://openalex.org/fields/31","display_name":"Physics and Astronomy"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.01119999960064888,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/protein-kinase-a","display_name":"protein kinase A","score":0.9670000076293945},{"id":"https://openalex.org/keywords/allosteric-activation","display_name":"allosteric activation","score":0.8790000081062317},{"id":"https://openalex.org/keywords/cyclic-nucleotide-binding-domain","display_name":"cyclic nucleotide-binding domain","score":0.7509999871253967},{"id":"https://openalex.org/keywords/regulatory-subunit","display_name":"regulatory subunit","score":0.828000009059906},{"id":"https://openalex.org/keywords/camp-binding","display_name":"cAMP binding","score":0.9369999766349792},{"id":"https://openalex.org/keywords/cooperative-binding","display_name":"cooperative binding","score":0.5669999718666077},{"id":"https://openalex.org/keywords/interdomain-interactions","display_name":"domain-domain interactions","score":0.7409999966621399},{"id":"https://openalex.org/keywords/protein-unfolding","display_name":"protein unfolding","score":0.6290000081062317},{"id":"https://openalex.org/keywords/protein-stability","display_name":"protein stability","score":0.7459999918937683},{"id":"https://openalex.org/keywords/single-molecule-force-spectroscopy","display_name":"single-molecule force spectroscopy","score":0.7170000076293945}],"concepts":[{"id":"https://openalex.org/C166342909","wikidata":"https://www.wikidata.org/wiki/Q845326","display_name":"Allosteric regulation","level":3,"score":0.8502820134162903},{"id":"https://openalex.org/C166014724","wikidata":"https://www.wikidata.org/wiki/Q337217","display_name":"Cooperativity","level":2,"score":0.7054692506790161},{"id":"https://openalex.org/C104292427","wikidata":"https://www.wikidata.org/wiki/Q899781","display_name":"Protein subunit","level":3,"score":0.6997864246368408},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6315008401870728},{"id":"https://openalex.org/C119473381","wikidata":"https://www.wikidata.org/wiki/Q49298","display_name":"Force spectroscopy","level":3,"score":0.5988339781761169},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.5952701568603516},{"id":"https://openalex.org/C187250156","wikidata":"https://www.wikidata.org/wiki/Q2902610","display_name":"Cooperative binding","level":3,"score":0.5581978559494019},{"id":"https://openalex.org/C97029542","wikidata":"https://www.wikidata.org/wiki/Q281417","display_name":"Protein kinase A","level":3,"score":0.41501182317733765},{"id":"https://openalex.org/C107824862","wikidata":"https://www.wikidata.org/wiki/Q616005","display_name":"Binding site","level":2,"score":0.37812358140945435},{"id":"https://openalex.org/C32909587","wikidata":"https://www.wikidata.org/wiki/Q11369","display_name":"Molecule","level":2,"score":0.34960949420928955},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.3317522406578064},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.26520663499832153},{"id":"https://openalex.org/C184235292","wikidata":"https://www.wikidata.org/wiki/Q421851","display_name":"Kinase","level":2,"score":0.23889771103858948},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.20663189888000488},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.12838739156723022},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1096/fasebj.30.1_supplement.856.5","doi":"https://doi.org/10.1096/fasebj.30.1_supplement.856.5","is_oa":false,"landing_page_url":"https://doi.org/10.1096/fasebj.30.1_supplement.856.5","pdf_url":null,"source":{"id":"https://openalex.org/S25293849","display_name":"The FASEB Journal","issn_l":"0892-6638","issn":["0892-6638","1530-6860"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FASEB Journal","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2883145667","https://openalex.org/W4294577593","https://openalex.org/W3005413682","https://openalex.org/W2972166720","https://openalex.org/W2935990424","https://openalex.org/W4225304627","https://openalex.org/W3175872846","https://openalex.org/W2791676160","https://openalex.org/W4372202566","https://openalex.org/W2011105856"],"abstract_inverted_index":{"Protein":[0],"Kinase":[1],"A":[2],"(PKA)":[3],"is":[4,24,50,166],"composed":[5],"of":[6,22,30,47,84,105,111,128,153,171,178,215,269],"Catalytic":[7],"(C)":[8],"and":[9,38,60,82,115,143,210,218,239,253,266],"Regulatory":[10],"(R)":[11],"subunits":[12],"that":[13,124,163],"assemble":[14],"as":[15,89,94,151,188],"an":[16],"inactive":[17],"holoenzyme.":[18],"The":[19,45,196],"allosteric":[20,204,247,271],"activation":[21],"PKA":[23],"triggered":[25,249],"by":[26,168,190,250,254],"the":[27,42,61,76,80,85,97,103,109,117,126,130,140,148,154,164,169,182,185,207,211,216,221,224,228,241,246,264],"cooperative":[28],"binding":[29,35,107,252],"cAMP":[31,106,179,251],"to":[32,51,73,174,237],"two":[33],"cyclic\u2010nucleotide":[34],"domains":[36,150,226],"(CNB\u2010A":[37],"CNB\u2010B)":[39],"located":[40],"in":[41,96,125,227,277],"R":[43,98,155,229],"subunit.":[44,99,230],"goal":[46],"this":[48,261],"study":[49,262],"establish":[52],"a":[53,199],"quantitative":[54,267],"relationship":[55],"between":[56,64,120,184,223,257],"protein":[57],"stability,":[58],"conformation":[59,83],"observed":[62,138,276],"cooperativity":[63],"CNB":[65,86,113,149,186,225,258],"domains.":[66,259],"We":[67,122],"used":[68],"optical":[69],"tweezers":[70],"force":[71,214],"spectroscopy":[72],"probe":[74],"at":[75,133,158],"single":[77],"molecule":[78],"level":[79],"stability":[81,110],"domains,":[87,187],"either":[88],"isolated":[90],"structures":[91],"or":[92],"together":[93],"found":[95,123],"Moreover,":[100,260],"we":[101],"investigated":[102],"effect":[104],"on":[108,116],"each":[112],"domain":[114],"interaction":[118,183,222],"energy":[119],"them.":[121],"absence":[127],"cAMP,":[129],"CNB\u2010A":[131,172],"unfolds":[132],"forces":[134,160,193],"higher":[135,191],"than":[136],"those":[137],"for":[139,203],"CNB\u2010B":[141,165],"(~10pN":[142],"~6pN,":[144],"respectively).":[145],"Interestingly,":[146],"however,":[147],"part":[152],"subunit":[156],"unfold":[157],"similar":[159],"(~10pN),":[161],"indicating":[162],"stabilized":[167],"presence":[170],"due":[173],"inter\u2010domain":[175,255],"interactions.":[176],"Binding":[177],"further":[180],"stabilizes":[181],"evidenced":[189],"unfolding":[192,213],"near":[194],"~16pN.":[195],"mutant":[197],"R241A,":[198],"hotspot":[200],"residue":[201],"position":[202],"interactions,":[205],"reduces":[206],"folding":[208],"probability":[209],"mean":[212],"CNB\u2010B,":[217],"severely":[219],"disrupts":[220],"Altogether,":[231],"our":[232],"experimental":[233],"strategy":[234],"allowed":[235],"us":[236],"trace":[238],"dissect":[240],"energetic":[242],"bases":[243],"associated":[244,273],"with":[245,274],"interactions":[248,256],"enabled":[263],"identification":[265],"characterization":[268],"aberrant":[270],"events":[272],"mutations":[275],"disease":[278],"states.":[279]},"counts_by_year":[],"updated_date":"2026-10-30T12:39:25.321706","created_date":"2023-11-27T00:00:00"}