{"id":"https://openalex.org/W4389024813","doi":"https://doi.org/10.1096/fasebj.31.1_supplement.1074.1","title":"Studies on the Physical Properties of the Pseudomonas aeruginosa \u2010induced Endothelial Prion Amyloid Cytotoxin(s)","display_name":"Studies on the Physical Properties of the Pseudomonas aeruginosa \u2010induced Endothelial Prion Amyloid Cytotoxin(s)","publication_year":2017,"publication_date":"2017-04-01","ids":{"openalex":"https://openalex.org/W4389024813","doi":"https://doi.org/10.1096/fasebj.31.1_supplement.1074.1"},"language":"en","primary_location":{"id":"doi:10.1096/fasebj.31.1_supplement.1074.1","doi":"https://doi.org/10.1096/fasebj.31.1_supplement.1074.1","is_oa":false,"landing_page_url":"https://doi.org/10.1096/fasebj.31.1_supplement.1074.1","pdf_url":null,"source":{"id":"https://openalex.org/S25293849","display_name":"The FASEB Journal","issn_l":"0892-6638","issn":["0892-6638","1530-6860"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FASEB Journal","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5041487639","display_name":"Kyle Adam Morrow","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2799524434","display_name":"Alabama College of Osteopathic Medicine","ror":"https://ror.org/02rzdts70","country_code":"US","type":"education","lineage":["https://openalex.org/I2799524434"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kyle Adam Morrow","raw_affiliation_strings":["Anatomy and Molecular Medicine, Alabama College of Osteopathic Medicine, Dothan, AL"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Anatomy and Molecular Medicine, Alabama College of Osteopathic Medicine, Dothan, AL","institution_ids":["https://openalex.org/I2799524434"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103860805","display_name":"Trevor C. Stevens","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I83809506","display_name":"University of South Alabama","ror":"https://ror.org/01s7b5y08","country_code":"US","type":"education","lineage":["https://openalex.org/I83809506"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Trevor C Stevens","raw_affiliation_strings":["Internal Medicine University of South Alabama Center for Lung Biology Mobile AL","Physiology and Cell Biology, University of South Alabama, Center for Lung Biology, Mobile, AL"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Internal Medicine University of South Alabama Center for Lung Biology Mobile AL","institution_ids":["https://openalex.org/I83809506"]},{"raw_affiliation_string":"Physiology and Cell Biology, University of South Alabama, Center for Lung Biology, Mobile, AL","institution_ids":["https://openalex.org/I83809506"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5074915080","display_name":"Ezinne Agwaramgbo","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I83809506","display_name":"University of South Alabama","ror":"https://ror.org/01s7b5y08","country_code":"US","type":"education","lineage":["https://openalex.org/I83809506"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ezinne Agwaramgbo","raw_affiliation_strings":["Physiology and Cell Biology, University of South Alabama, Center for Lung Biology, Mobile, AL"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Physiology and Cell Biology, University of South Alabama, Center for Lung Biology, Mobile, AL","institution_ids":["https://openalex.org/I83809506"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5056759943","display_name":"Geri Langham","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I83809506","display_name":"University of South Alabama","ror":"https://ror.org/01s7b5y08","country_code":"US","type":"education","lineage":["https://openalex.org/I83809506"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Geri Langham","raw_affiliation_strings":["Physiology and Cell Biology, University of South Alabama, Center for Lung Biology, Mobile, AL"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Physiology and Cell Biology, University of South Alabama, Center for Lung Biology, Mobile, AL","institution_ids":["https://openalex.org/I83809506"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Michael Francis","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I83809506","display_name":"University of South Alabama","ror":"https://ror.org/01s7b5y08","country_code":"US","type":"education","lineage":["https://openalex.org/I83809506"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michael Francis","raw_affiliation_strings":["Physiology and Cell Biology, University of South Alabama, Center for Lung Biology, Mobile, AL"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Physiology and Cell Biology, University of South Alabama, Center for Lung Biology, Mobile, AL","institution_ids":["https://openalex.org/I83809506"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109858560","display_name":"Ron Balczon","orcid":"https://orcid.org/0000-0001-8263-7221","observed_orcids":["https://orcid.org/0000-0001-8263-7221"]},"institutions":[{"id":"https://openalex.org/I83809506","display_name":"University of South Alabama","ror":"https://ror.org/01s7b5y08","country_code":"US","type":"education","lineage":["https://openalex.org/I83809506"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ron Balczon","raw_affiliation_strings":["Biochemistry and Molecular Biology, University of South Alabama, Center for Lung Biology, Mobile, AL"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biochemistry and Molecular Biology, University of South Alabama, Center for Lung Biology, Mobile, AL","institution_ids":["https://openalex.org/I83809506"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5048696645","display_name":"Troy Stevens","orcid":"https://orcid.org/0000-0001-9689-929X","observed_orcids":["https://orcid.org/0000-0001-9689-929X"]},"institutions":[{"id":"https://openalex.org/I83809506","display_name":"University of South Alabama","ror":"https://ror.org/01s7b5y08","country_code":"US","type":"education","lineage":["https://openalex.org/I83809506"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Troy Stevens","raw_affiliation_strings":["Internal Medicine, University of South Alabama, Center for Lung Biology, Mobile, AL","Physiology and Cell Biology, University of South Alabama, Center for Lung Biology, Mobile, AL"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Internal Medicine, University of South Alabama, Center for Lung Biology, Mobile, AL","institution_ids":["https://openalex.org/I83809506"]},{"raw_affiliation_string":"Physiology and Cell Biology, University of South Alabama, Center for Lung Biology, Mobile, AL","institution_ids":["https://openalex.org/I83809506"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3740,"currency":"USD","value_usd":3740},"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.28652296,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"31","issue":"S1","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11335","display_name":"Prion Diseases and Protein Misfolding","score":0.8471999764442444,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11335","display_name":"Prion Diseases and Protein Misfolding","score":0.8471999764442444,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10086","display_name":"Alzheimer's disease research and treatments","score":0.1023000031709671,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.005799999926239252,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/pseudomonas-aeruginosa","display_name":"Pseudomonas aeruginosa","score":0.9860000014305115},{"id":"https://openalex.org/keywords/endothelial-cells","display_name":"endothelial cells","score":0.6029999852180481},{"id":"https://openalex.org/keywords/pulmonary-infection","display_name":"pulmonary infection","score":0.6430000066757202},{"id":"https://openalex.org/keywords/angular-velocity","display_name":"angular velocity","score":0.7820000052452087},{"id":"https://openalex.org/keywords/protease-resistance","display_name":"protease resistance","score":0.7200000286102295},{"id":"https://openalex.org/keywords/proteinase-k","display_name":"proteinase K","score":0.9020000100135803},{"id":"https://openalex.org/keywords/diethyl-pyrocarbonate","display_name":"diethylpyrocarbonate","score":0.9120000004768372},{"id":"https://openalex.org/keywords/post-translational-modification","display_name":"post-translational modification","score":0.9509999752044678},{"id":"https://openalex.org/keywords/cytotoxicity","display_name":"cytotoxicity","score":0.8270000219345093},{"id":"https://openalex.org/keywords/capillary-endothelium","display_name":"capillary endothelium","score":0.6129999756813049},{"id":"https://openalex.org/keywords/bacterial-virulence-factors","display_name":"bacterial virulence factors","score":0.5929999947547913}],"concepts":[{"id":"https://openalex.org/C7623868","wikidata":"https://www.wikidata.org/wiki/Q1967135","display_name":"Centrifugation","level":2,"score":0.6589614152908325},{"id":"https://openalex.org/C2776714187","wikidata":"https://www.wikidata.org/wiki/Q212410","display_name":"Protease","level":3,"score":0.5907217860221863},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5872983336448669},{"id":"https://openalex.org/C2778471508","wikidata":"https://www.wikidata.org/wiki/Q425310","display_name":"Proteinase K","level":3,"score":0.5418996810913086},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4861471354961395},{"id":"https://openalex.org/C2777633098","wikidata":"https://www.wikidata.org/wiki/Q2736051","display_name":"Amyloid (mycology)","level":2,"score":0.45489591360092163},{"id":"https://openalex.org/C132946820","wikidata":"https://www.wikidata.org/wiki/Q2509459","display_name":"Differential centrifugation","level":2,"score":0.41645312309265137},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.404078871011734},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3289355933666229},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2299709916114807},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.20285353064537048},{"id":"https://openalex.org/C179104552","wikidata":"https://www.wikidata.org/wiki/Q11165","display_name":"Inorganic chemistry","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1096/fasebj.31.1_supplement.1074.1","doi":"https://doi.org/10.1096/fasebj.31.1_supplement.1074.1","is_oa":false,"landing_page_url":"https://doi.org/10.1096/fasebj.31.1_supplement.1074.1","pdf_url":null,"source":{"id":"https://openalex.org/S25293849","display_name":"The FASEB Journal","issn_l":"0892-6638","issn":["0892-6638","1530-6860"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FASEB Journal","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.9391000270843506,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G7109376089","display_name":"Endothelial tau oligomerization causes transmissible vascular cytototoxicity","funder_award_id":"16POST27250139","funder_id":"https://openalex.org/F4320306230","funder_display_name":"American Heart Association"},{"id":"https://openalex.org/G9000152375","display_name":null,"funder_award_id":"HL\u201060024","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G9000168736","display_name":null,"funder_award_id":"HL66299","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"}],"funders":[{"id":"https://openalex.org/F4320306230","display_name":"American Heart Association","ror":"https://ror.org/013kjyp64"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2596539925","https://openalex.org/W2884733365","https://openalex.org/W3091954696","https://openalex.org/W3025265821","https://openalex.org/W7084406562","https://openalex.org/W4389019445","https://openalex.org/W2275549450","https://openalex.org/W1847523822","https://openalex.org/W3170020262","https://openalex.org/W3173832487"],"abstract_inverted_index":{"Rationale":[0],"Pseudomonas":[1],"aeruginosa":[2,78,365],"lung":[3],"infection":[4,366],"targets":[5],"capillary":[6],"endothelium":[7],"and":[8,29,32,38,61,68,110,123,129,157,167,195,198,251,260,263,383,399],"induces":[9],"the":[10,56,283,287,301,351,360],"production":[11,372],"of":[12,46,65,183,223,254,282,312,350,367,373,396],"prion":[13,376],"amyloid":[14,23,48,57,315,353,377],"cytotoxin(s),":[15],"including":[16],"a":[17,146,207,313,374],"high":[18],"molecular":[19],"weight":[20],"tau.":[21],"These":[22],"proteins":[24],"are":[25,36],"heat":[26],"stable,":[27],"RNase":[28],"DNase":[30],"insensitive,":[31,323],"protease":[33,66,127,322,381],"resistant.":[34],"They":[35],"self\u2010replicating":[37],"transmissible":[39],"among":[40],"na\u00efve":[41],"cells.":[42],"The":[43,221,317],"physical":[44],"nature":[45],"these":[47,187],"cytotoxins":[49],"remains":[50],"incompletely":[51],"resolved.":[52],"Here,":[53],"we":[54],"examined":[55],"cytotoxin's":[58],"angular":[59,284],"velocity,":[60],"determined":[62],"its":[63,334,357],"degree":[64],"sensitivity":[67],"responsiveness":[69],"to":[70,83,153,193,214,225,234,332,356,385],"inactivation":[71],"by":[72],"post\u2010translational":[73,131,181,386],"modification.":[74],"Methods":[75],"A":[76],"P.":[77,364],"strain,":[79],"PA103,":[80],"was":[81,98,117,151,170,191,231,320,330],"used":[82,206],"infect":[84],"pulmonary":[85,368],"microvascular":[86],"endothelial":[87,318,352,369],"cells":[88,370],"(PMVECs)":[89],"for":[90,104,121,126,130,161,174,180,210],"4":[91],"hours,":[92],"at":[93,107,140,177],"which":[94,342],"time":[95,148],"cell":[96,236],"supernatant":[97,173,190],"collected,":[99],"centrifuged":[100],"(4,500":[101],"\u00d7":[102,142,296,305],"g":[103,143],"10":[105,166,297,306,307],"minutes":[106,176],"room":[108,178],"temperature),":[109],"filter":[111],"sterilized":[112],"(0.22":[113],"\u03bcm":[114],"filter).":[115],"Supernatant":[116,150,240],"divided":[118],"into":[119],"aliquots":[120],"centrifugation":[122,255,269,277],"sedimentation":[124],"experiments,":[125],"treatment,":[128],"modification":[132,182,387],"using":[133,388],"diethylpyrocarbonate":[134],"(DEPC).":[135],"Centrifugation":[136],"experiments":[137],"were":[138,256,265],"conducted":[139],"150,000":[141],"(4\u00b0C)":[144],"over":[145],"24\u2010hour":[147,325],"course.":[149],"exposed":[152],"proteinase":[154,328],"K":[155,329],"(100":[156],"500":[158],"\u03bcg/mL)":[159],"digestion":[160],"24":[162,252],"hours.":[163],"DEPC":[164,339],"(1,":[165],"20":[168],"mM)":[169],"incubated":[171],"with":[172,310,327,338],"30":[175],"temperature":[179],"histidine":[184,345],"residues.":[185],"After":[186],"independent":[188],"treatments,":[189],"added":[192],"PMVECs":[194],"gap":[196,237,261],"formation":[197,262],"cytotoxicity":[199,264,349],"(e.g.":[200,244],"LDH":[201],"release":[202],"assay)":[203],"determined.":[204],"We":[205],"custom":[208],"plugin":[209],"ImageJ":[211],"(NIH)":[212],"software":[213],"automatically":[215],"quantify":[216],"intercellular":[217],"gaps":[218],"in":[219],"images.":[220],"ratio":[222],"black":[224],"white":[226],"pixels":[227],"within":[228],"each":[229],"image":[230],"then":[232],"measured":[233],"determine":[235],"area.":[238],"Results":[239],"samples":[241],"from":[242],"0":[243],"control),":[245],"1,":[246],"2,":[247],"4,":[248],"8,":[249],"16":[250],"hours":[253,268,276],"placed":[257],"on":[258,359],"PMVECs,":[259],"measured.":[266],"0\u20134":[267],"did":[270],"not":[271],"reduce":[272],"cytotoxicity,":[273],"whereas":[274],"8\u201324":[275],"nearly":[278],"eliminated":[279,348],"cytotoxicity.":[280,335],"Calculation":[281],"velocity":[285],"revealed":[286],"endothelial\u2010derived":[288],"cytotoxin":[289,319,378],"is":[290,380],"small":[291],"and/or":[292],"less":[293],"dense":[294],"(1.14":[295],"12":[298],")":[299],"than":[300],"scrapie":[302,361],"agent":[303],"(3":[304],"),":[308],"consistent":[309],"identification":[311],"novel":[314,375],"cytotoxin.":[316],"extremely":[321],"as":[324],"treatment":[326,337],"insufficient":[331],"eliminate":[333],"However,":[336],"(10":[340],"mM),":[341],"post\u2010translationally":[343],"modifies":[344],"side":[346],"chains,":[347],"cytotoxin,":[354],"similar":[355],"effect":[358],"agent.":[362],"Conclusion":[363],"causes":[371],"that":[379],"resistant":[382],"sensitive":[384],"DEPC.":[389],"Support":[390],"or":[391],"Funding":[392],"Information":[393],"National":[394],"Institutes":[395],"Health":[397],"HL66299":[398],"HL60024,":[400],"American":[401],"Heart":[402],"Association":[403],"16POST27250139":[404]},"counts_by_year":[],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2023-11-27T00:00:00"}
