{"id":"https://openalex.org/W4389022918","doi":"https://doi.org/10.1096/fasebj.31.1_supplement.600.14","title":"Characterization of a Short Motif Capable of Enhancing Human Cap\u2010Independent Translation","display_name":"Characterization of a Short Motif Capable of Enhancing Human Cap\u2010Independent Translation","publication_year":2017,"publication_date":"2017-04-01","ids":{"openalex":"https://openalex.org/W4389022918","doi":"https://doi.org/10.1096/fasebj.31.1_supplement.600.14"},"language":"en","primary_location":{"id":"doi:10.1096/fasebj.31.1_supplement.600.14","doi":"https://doi.org/10.1096/fasebj.31.1_supplement.600.14","is_oa":false,"landing_page_url":"https://doi.org/10.1096/fasebj.31.1_supplement.600.14","pdf_url":null,"source":{"id":"https://openalex.org/S25293849","display_name":"The FASEB Journal","issn_l":"0892-6638","issn":["0892-6638","1530-6860"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FASEB Journal","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5088710200","display_name":"Amber N. Juba","orcid":"https://orcid.org/0000-0002-3782-8560","observed_orcids":["https://orcid.org/0000-0002-3782-8560"]},"institutions":[{"id":"https://openalex.org/I128902783","display_name":"Midwestern University","ror":"https://ror.org/046yatd98","country_code":"US","type":"education","lineage":["https://openalex.org/I128902783"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Amber N. Juba","raw_affiliation_strings":["Biomedical Sciences Program, Midwestern University, Glendale, AZ"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biomedical Sciences Program, Midwestern University, Glendale, AZ","institution_ids":["https://openalex.org/I128902783"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Allison Hansen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I128902783","display_name":"Midwestern University","ror":"https://ror.org/046yatd98","country_code":"US","type":"education","lineage":["https://openalex.org/I128902783"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Allison Hansen","raw_affiliation_strings":["Biomedical Sciences Program, Midwestern University, Glendale, AZ"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biomedical Sciences Program, Midwestern University, Glendale, AZ","institution_ids":["https://openalex.org/I128902783"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5081890628","display_name":"John Charles Chaput","orcid":"https://orcid.org/0000-0003-1393-135X","observed_orcids":["https://orcid.org/0000-0003-1393-135X"]},"institutions":[{"id":"https://openalex.org/I55732556","display_name":"Arizona State University","ror":"https://ror.org/03efmqc40","country_code":"US","type":"education","lineage":["https://openalex.org/I55732556"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"John C. Chaput","raw_affiliation_strings":["Center for Evolutionary Medicine and Informatics, The Biodesign Institute, Arizona State University, Tempe, AZ","Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Evolutionary Medicine and Informatics, The Biodesign Institute, Arizona State University, Tempe, AZ","institution_ids":["https://openalex.org/I55732556"]},{"raw_affiliation_string":"Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ","institution_ids":["https://openalex.org/I55732556"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5051931242","display_name":"Brian P. Wellensiek","orcid":"https://orcid.org/0000-0001-9068-5637","observed_orcids":["https://orcid.org/0000-0001-9068-5637"]},"institutions":[{"id":"https://openalex.org/I128902783","display_name":"Midwestern University","ror":"https://ror.org/046yatd98","country_code":"US","type":"education","lineage":["https://openalex.org/I128902783"]},{"id":"https://openalex.org/I55732556","display_name":"Arizona State University","ror":"https://ror.org/03efmqc40","country_code":"US","type":"education","lineage":["https://openalex.org/I55732556"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Brian P. Wellensiek","raw_affiliation_strings":["Biomedical Sciences Program, Midwestern University, Glendale, AZ","Center for Evolutionary Medicine and Informatics, The Biodesign Institute, Arizona State University, Tempe, AZ"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biomedical Sciences Program, Midwestern University, Glendale, AZ","institution_ids":["https://openalex.org/I128902783"]},{"raw_affiliation_string":"Center for Evolutionary Medicine and Informatics, The Biodesign Institute, Arizona State University, Tempe, AZ","institution_ids":["https://openalex.org/I55732556"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3740,"currency":"USD","value_usd":3740},"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.30356597,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"31","issue":"S1","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10952","display_name":"PI3K/AKT/mTOR signaling in cancer","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cap-independent-translation","display_name":"cap-independent translation","score":0.9909999966621399},{"id":"https://openalex.org/keywords/uncapped-mrna","display_name":"uncapped mRNA","score":0.7979999780654907},{"id":"https://openalex.org/keywords/translation-initiation","display_name":"translation initiation","score":0.699999988079071},{"id":"https://openalex.org/keywords/mrna-display","display_name":"mRNA display","score":0.5770000219345093},{"id":"https://openalex.org/keywords/translation-efficiency","display_name":"translation efficiency","score":0.6179999709129333},{"id":"https://openalex.org/keywords/open-reading-frames","display_name":"open reading frames","score":0.7120000123977661},{"id":"https://openalex.org/keywords/human-proteome","display_name":"human proteome","score":0.47699999809265137}],"concepts":[{"id":"https://openalex.org/C47289529","wikidata":"https://www.wikidata.org/wiki/Q908550","display_name":"Open reading frame","level":4,"score":0.6193456053733826},{"id":"https://openalex.org/C182325514","wikidata":"https://www.wikidata.org/wiki/Q7322425","display_name":"Ribosome profiling","level":5,"score":0.5970940589904785},{"id":"https://openalex.org/C89604277","wikidata":"https://www.wikidata.org/wiki/Q424345","display_name":"Untranslated region","level":4,"score":0.5946335792541504},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.572202742099762},{"id":"https://openalex.org/C149364088","wikidata":"https://www.wikidata.org/wiki/Q185917","display_name":"Translation (biology)","level":4,"score":0.5666342973709106},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.5532063841819763},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.5332199335098267},{"id":"https://openalex.org/C77430603","wikidata":"https://www.wikidata.org/wiki/Q166992","display_name":"Internal ribosome entry site","level":5,"score":0.4877406060695648},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.48724570870399475},{"id":"https://openalex.org/C117745874","wikidata":"https://www.wikidata.org/wiki/Q901612","display_name":"Sequence motif","level":3,"score":0.48423099517822266},{"id":"https://openalex.org/C4718897","wikidata":"https://www.wikidata.org/wiki/Q5408698","display_name":"Eukaryotic translation","level":5,"score":0.4705548584461212},{"id":"https://openalex.org/C39685955","wikidata":"https://www.wikidata.org/wiki/Q3455905","display_name":"Translational regulation","level":5,"score":0.4324970841407776},{"id":"https://openalex.org/C88478588","wikidata":"https://www.wikidata.org/wiki/Q42244","display_name":"Ribosome","level":4,"score":0.4306766986846924},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.41054531931877136},{"id":"https://openalex.org/C12464233","wikidata":"https://www.wikidata.org/wiki/Q937624","display_name":"Five prime untranslated region","level":5,"score":0.4102451205253601},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3995952010154724},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.23555821180343628},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.16849228739738464}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1096/fasebj.31.1_supplement.600.14","doi":"https://doi.org/10.1096/fasebj.31.1_supplement.600.14","is_oa":false,"landing_page_url":"https://doi.org/10.1096/fasebj.31.1_supplement.600.14","pdf_url":null,"source":{"id":"https://openalex.org/S25293849","display_name":"The FASEB Journal","issn_l":"0892-6638","issn":["0892-6638","1530-6860"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FASEB Journal","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W3016775315","https://openalex.org/W3174392823","https://openalex.org/W2006798984","https://openalex.org/W2899490838","https://openalex.org/W2123174499","https://openalex.org/W2082824227","https://openalex.org/W2139568440","https://openalex.org/W2234324103","https://openalex.org/W2921428550","https://openalex.org/W2193326420"],"abstract_inverted_index":{"The":[0],"classical":[1],"model":[2],"for":[3,34,102,268,339],"eukaryotic":[4],"translation":[5,74,110,180,189,228,257,269,306,324],"initiation":[6,37],"involves":[7],"linear":[8],"scanning,":[9],"a":[10,18,40,80,252,314,352],"cap\u2010dependent":[11,58],"process":[12],"in":[13,127,161,186,234,237,301],"which":[14,69,193],"the":[15,21,25,30,66,99,109,130,142,149,163,169,179,200,210,216,219,224,245,274,279,291,302,308,319,331],"ribosome":[16],"recognizes":[17],"7\u2010methylguanosinecap":[19],"at":[20],"5\u2032":[22,31],"end":[23],"of":[24,43,73,111,116,129,152,183,206,218,223,304,322,356],"RNA":[26,91,103],"message":[27],"and":[28,221,236,240,278,327,364],"scans":[29],"untranslated":[32],"region":[33],"an":[35,50,153,158,298,365],"appropriate":[36],"site.":[38],"Alternatively,":[39],"growing":[41],"body":[42],"evidence":[44,289],"suggests":[45],"that":[46,106,124,141,244,290],"cap\u2010independent":[47,71,305],"mechanisms":[48],"play":[49],"important":[51,299],"role":[52,160,176,300],"during":[53],"many":[54,128],"cellular":[55],"events":[56],"where":[57],"scanning":[59],"is":[60],"inhibited.":[61],"However":[62],"despite":[63],"this":[64,70,87,134,175,340],"evidence,":[65],"mechanism":[67,303],"by":[68,88,168,259,344],"mode":[72],"occurs":[75],"remains":[76],"largely":[77],"unknown.":[78],"In":[79],"previous":[81],"study,":[82],"we":[83,139,177],"began":[84],"to":[85,97,214,273,317,330,350,362,369],"address":[86],"combining":[89],"messenger":[90],"display":[92],"with":[93,271],"next\u2010generation":[94],"deep":[95],"sequencing":[96],"profile":[98],"human":[100,185,239,309,323,332],"genome":[101],"leader":[104,150,171],"sequences":[105,118],"can":[107],"enhance":[108],"uncapped":[112],"mRNA":[113],"transcripts.":[114],"Analysis":[115],"these":[117,137,286],"revealeda":[119],"short,":[120],"13":[121,144,201,246,275,294],"nucleotide":[122,202,247,276,295],"motif":[123,225,277,296],"was":[125,342],"present":[126,147,250],"RNAs":[131],"isolated":[132],"via":[133],"method.":[135],"Given":[136],"findings,":[138],"propose":[140],"identified":[143,265,292],"nucleotidemotif,":[145],"when":[146,249],"within":[148,251],"sequence":[151],"open":[154,281],"reading":[155,282],"frame,":[156],"has":[157],"integral":[159],"determining":[162],"increased":[164,255],"translational":[165],"activity":[166,182],"driven":[167],"larger":[170,253],"sequence.":[172],"To":[173],"explore":[174,318],"examined":[178],"enhancing":[181,190,325],"several":[184,260],"vitro":[187,235],"selected":[188],"elements":[191,326],"(TEEs),":[192],"either":[194],"contained":[195],"or":[196,335],"did":[197],"not":[198],"contain":[199],"motif.":[203],"A":[204],"series":[205],"reporter":[207],"vectors":[208],"containing":[209],"TEEs":[211],"were":[212],"used":[213],"assay":[215],"effect":[217],"presence":[220],"absence":[222],"on":[226],"overall":[227],"efficiency.":[229],"Functional":[230],"analysis":[231],"studies":[232,263],"performed":[233],"cultured":[238],"mammalian":[241],"cells":[242],"indicate":[243],"motif,":[248],"TEE,":[254],"protein":[256],"levels":[258],"fold.":[261],"Additional":[262],"have":[264],"distance":[266],"constraints":[267],"enhancement":[270],"respect":[272],"downstream":[280],"frame.":[283],"Taken":[284],"together":[285],"results":[287],"provide":[288],"short":[293],"plays":[297],"from":[307],"genome.":[310],"This":[311],"observation":[312],"provides":[313],"new":[315],"opportunity":[316],"mechanistic":[320],"details":[321],"their":[328],"contribution":[329],"proteome.":[333],"Support":[334],"Funding":[336,338],"Information":[337],"research":[341,359],"provided":[343],"Midwestern":[345,353],"University":[346,354],"intramural":[347],"funds":[348],"awarded":[349,361,368],"BPW,":[351],"College":[355],"Veterinary":[357],"Medicine":[358],"fellowship":[360],"AH,":[363],"NIH":[366],"grant":[367],"JCC":[370]},"counts_by_year":[],"updated_date":"2026-10-30T12:39:25.321706","created_date":"2023-11-27T00:00:00"}