{"id":"https://openalex.org/W4388894901","doi":"https://doi.org/10.17504/protocols.io.e6nvwjxnzlmk/v1","title":"DART-FISH Protocol v1","display_name":"DART-FISH Protocol v1","publication_year":2023,"publication_date":"2023-06-02","ids":{"openalex":"https://openalex.org/W4388894901","doi":"https://doi.org/10.17504/protocols.io.e6nvwjxnzlmk/v1"},"language":"en","primary_location":{"id":"doi:10.17504/protocols.io.e6nvwjxnzlmk/v1","doi":"https://doi.org/10.17504/protocols.io.e6nvwjxnzlmk/v1","is_oa":true,"landing_page_url":"https://doi.org/10.17504/protocols.io.e6nvwjxnzlmk/v1","pdf_url":"https://www.protocols.io/view/dart-fish-protocol-cu4awyse.pdf","source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"},"volume":null,"type":"preprint","indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.protocols.io/view/dart-fish-protocol-cu4awyse.pdf","any_repository_has_fulltext":null},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5111165728","display_name":"Chien-Ju Chen","orcid":"https://orcid.org/0000-0003-0876-9896","observed_orcids":["https://orcid.org/0000-0003-0876-9896"]},"institutions":[{"id":"https://openalex.org/I36258959","display_name":"University of California San Diego","ror":"https://ror.org/0168r3w48","country_code":"US","type":"education","lineage":["https://openalex.org/I36258959"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Chien-Ju Chen","raw_affiliation_strings":["University of California , San Diego Chien-Ju","University of California, San Diego"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of California , San Diego Chien-Ju","institution_ids":["https://openalex.org/I36258959"]},{"raw_affiliation_string":"University of California, San Diego","institution_ids":["https://openalex.org/I36258959"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5023791605","display_name":"Kian Kalhor","orcid":"https://orcid.org/0000-0001-9416-3078","observed_orcids":["https://orcid.org/0000-0001-9416-3078"]},"institutions":[{"id":"https://openalex.org/I36258959","display_name":"University of California San Diego","ror":"https://ror.org/0168r3w48","country_code":"US","type":"education","lineage":["https://openalex.org/I36258959"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kian Kalhor","raw_affiliation_strings":["University of California , San Diego Chien-Ju","University of California, San Diego"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of California , San Diego Chien-Ju","institution_ids":["https://openalex.org/I36258959"]},{"raw_affiliation_string":"University of California, San Diego","institution_ids":["https://openalex.org/I36258959"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5100342359","display_name":"Kun Zhang","orcid":"https://orcid.org/0000-0002-7596-5224","observed_orcids":["https://orcid.org/0000-0002-7596-5224"]},"institutions":[{"id":"https://openalex.org/I36258959","display_name":"University of California San Diego","ror":"https://ror.org/0168r3w48","country_code":"US","type":"education","lineage":["https://openalex.org/I36258959"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kun Zhang","raw_affiliation_strings":["University of California , San Diego Chien-Ju","University of California, San Diego"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of California , San Diego Chien-Ju","institution_ids":["https://openalex.org/I36258959"]},{"raw_affiliation_string":"University of California, San Diego","institution_ids":["https://openalex.org/I36258959"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I36258959"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.40380001068115234,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.40380001068115234,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.24729999899864197,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.1932000070810318,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/padlock-probes","display_name":"padlock probes","score":0.8399999737739563},{"id":"https://openalex.org/keywords/single-molecule-rna-detection","display_name":"single-molecule RNA detection","score":0.550000011920929},{"id":"https://openalex.org/keywords/spatial-transcriptomics","display_name":"spatial transcriptomics","score":0.703000009059906}],"concepts":[{"id":"https://openalex.org/C2779417484","wikidata":"https://www.wikidata.org/wiki/Q406009","display_name":"Dart","level":2,"score":0.7054837346076965},{"id":"https://openalex.org/C2909208804","wikidata":"https://www.wikidata.org/wiki/Q127282","display_name":"Fish <Actinopterygii>","level":2,"score":0.670665979385376},{"id":"https://openalex.org/C81439078","wikidata":"https://www.wikidata.org/wiki/Q2304142","display_name":"In situ hybridization","level":4,"score":0.6203455924987793},{"id":"https://openalex.org/C2777822432","wikidata":"https://www.wikidata.org/wiki/Q216681","display_name":"In situ","level":2,"score":0.5434167981147766},{"id":"https://openalex.org/C2780385302","wikidata":"https://www.wikidata.org/wiki/Q367158","display_name":"Protocol (science)","level":3,"score":0.5126156210899353},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.47383078932762146},{"id":"https://openalex.org/C19275194","wikidata":"https://www.wikidata.org/wiki/Q222903","display_name":"Multiplexing","level":2,"score":0.4410251975059509},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.42542269825935364},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.31393367052078247},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.21946066617965698},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.14385893940925598},{"id":"https://openalex.org/C505870484","wikidata":"https://www.wikidata.org/wiki/Q180538","display_name":"Fishery","level":1,"score":0.13087254762649536},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.09170287847518921},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.07414773106575012},{"id":"https://openalex.org/C76155785","wikidata":"https://www.wikidata.org/wiki/Q418","display_name":"Telecommunications","level":1,"score":0.07000049948692322},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C204787440","wikidata":"https://www.wikidata.org/wiki/Q188504","display_name":"Alternative medicine","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.17504/protocols.io.e6nvwjxnzlmk/v1","doi":"https://doi.org/10.17504/protocols.io.e6nvwjxnzlmk/v1","is_oa":true,"landing_page_url":"https://doi.org/10.17504/protocols.io.e6nvwjxnzlmk/v1","pdf_url":"https://www.protocols.io/view/dart-fish-protocol-cu4awyse.pdf","source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.17504/protocols.io.e6nvwjxnzlmk/v1","doi":"https://doi.org/10.17504/protocols.io.e6nvwjxnzlmk/v1","is_oa":true,"landing_page_url":"https://doi.org/10.17504/protocols.io.e6nvwjxnzlmk/v1","pdf_url":"https://www.protocols.io/view/dart-fish-protocol-cu4awyse.pdf","source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"score":0.6600000262260437,"id":"https://metadata.un.org/sdg/14","display_name":"Life below water"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4388894901.pdf","grobid_xml":"https://content.openalex.org/works/W4388894901.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4392973308","https://openalex.org/W3109948114","https://openalex.org/W6894308839","https://openalex.org/W4280553101","https://openalex.org/W3016045909","https://openalex.org/W4387715254","https://openalex.org/W4388916750","https://openalex.org/W3133643758","https://openalex.org/W3004621765","https://openalex.org/W3012098381"],"abstract_inverted_index":{"In":[0],"the":[1,42],"manuscript":[2],"Mapping":[3],"Human":[4],"Tissues":[5],"with":[6],"Highly":[7],"Multiplexed":[8],"RNA":[9],"in":[10,19,25,32],"situ":[11,20,26],"Hybridization":[12],"(https://doi.org/10.1101/2023.08.16.553610),":[13],"we":[14],"describe":[15],"a":[16],"highly":[17],"multiplexed":[18],"hybridization":[21],"technique":[22],"based":[23],"on":[24],"padlock":[27],"probe":[28],"capture":[29],"and":[30,45],"demonstrate":[31],"applicability":[33],"to":[34],"different":[35],"human":[36],"tissue":[37],"types.":[38],"This":[39],"protocol":[40],"details":[41],"rolony":[43],"generation":[44],"decoding":[46],"steps":[47],"of":[48],"DART-FISH.":[49]},"counts_by_year":[],"updated_date":"2026-10-28T10:33:24.094632","created_date":"2023-11-23T00:00:00"}