{"id":"https://openalex.org/W4388702109","doi":"https://doi.org/10.1177/23978473231215122","title":"Safety evaluations of alternansucrase enzyme expressed in Escherichia coli shows no adverse effects invivo and invitro","display_name":"Safety evaluations of alternansucrase enzyme expressed in Escherichia coli shows no adverse effects invivo and invitro","publication_year":2023,"publication_date":"2023-01-01","ids":{"openalex":"https://openalex.org/W4388702109","doi":"https://doi.org/10.1177/23978473231215122"},"language":"en","primary_location":{"id":"doi:10.1177/23978473231215122","doi":"https://doi.org/10.1177/23978473231215122","is_oa":true,"landing_page_url":"http://dx.doi.org/10.1177/23978473231215122","pdf_url":null,"source":{"id":"https://openalex.org/S4210173702","display_name":"Toxicology Research and Application","issn_l":"2397-8473","issn":["2397-8473"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310320017","host_organization_name":"SAGE Publishing","host_organization_lineage":["https://openalex.org/P4310320017"],"host_organization_lineage_names":["SAGE Publishing"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Toxicology Research and Application","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"http://dx.doi.org/10.1177/23978473231215122","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101489271","display_name":"Ankit Kishor Rathi","orcid":"https://orcid.org/0000-0002-7173-5587","observed_orcids":["https://orcid.org/0000-0002-7173-5587"]},"institutions":[{"id":"https://openalex.org/I4405272632","display_name":"Advanced Enzyme Technologies Limited (India)","ror":"https://ror.org/04fkxmv96","country_code":"IN","type":"company","lineage":["https://openalex.org/I4405272632"]}],"countries":["IN"],"is_corresponding":true,"raw_author_name":"Ankit Rathi","raw_affiliation_strings":["Advanced Enzyme Technologies Limited, Thane, India"],"raw_orcid":"https://orcid.org/0000-0002-7173-5587","affiliations":[{"raw_affiliation_string":"Advanced Enzyme Technologies Limited, Thane, India","institution_ids":["https://openalex.org/I4405272632"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Harshada Chandan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405272632","display_name":"Advanced Enzyme Technologies Limited (India)","ror":"https://ror.org/04fkxmv96","country_code":"IN","type":"company","lineage":["https://openalex.org/I4405272632"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Harshada Chandan","raw_affiliation_strings":["Advanced Enzyme Technologies Limited, Thane, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Advanced Enzyme Technologies Limited, Thane, India","institution_ids":["https://openalex.org/I4405272632"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5055578084","display_name":"Chaitali Vira","orcid":"https://orcid.org/0000-0002-9729-9541","observed_orcids":["https://orcid.org/0000-0002-9729-9541"]},"institutions":[{"id":"https://openalex.org/I4405272632","display_name":"Advanced Enzyme Technologies Limited (India)","ror":"https://ror.org/04fkxmv96","country_code":"IN","type":"company","lineage":["https://openalex.org/I4405272632"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Chaitali Vira","raw_affiliation_strings":["Advanced Enzyme Technologies Limited, Sinnar, India"],"raw_orcid":"https://orcid.org/0000-0002-9729-9541","affiliations":[{"raw_affiliation_string":"Advanced Enzyme Technologies Limited, Sinnar, India","institution_ids":["https://openalex.org/I4405272632"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5073746896","display_name":"Mukul P Pore","orcid":"https://orcid.org/0000-0001-8104-2330","observed_orcids":["https://orcid.org/0000-0001-8104-2330"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Mukul P Pore","raw_affiliation_strings":["Intox Pvt. Ltd, Pune, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Intox Pvt. Ltd, Pune, India","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5101489271"],"corresponding_institution_ids":["https://openalex.org/I4405272632"],"apc_list":{"value":1200,"currency":"USD","value_usd":1200},"apc_paid":{"value":1200,"currency":"USD","value_usd":1200},"fwci":0.2277,"has_fulltext":false,"cited_by_count":2,"citation_normalized_percentile":{"value":0.4513016,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":95},"biblio":{"volume":"7","issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T13165","display_name":"Agricultural safety and regulations","score":0.6478999853134155,"subfield":{"id":"https://openalex.org/subfields/1106","display_name":"Food Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T13165","display_name":"Agricultural safety and regulations","score":0.6478999853134155,"subfield":{"id":"https://openalex.org/subfields/1106","display_name":"Food Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10433","display_name":"Carcinogens and Genotoxicity Assessment","score":0.3019999861717224,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12489","display_name":"Microbial Metabolites in Food Biotechnology","score":0.009800000116229057,"subfield":{"id":"https://openalex.org/subfields/2916","display_name":"Nutrition and Dietetics"},"field":{"id":"https://openalex.org/fields/29","display_name":"Nursing"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/glucansucrase","display_name":"glucansucrase","score":0.9639999866485596},{"id":"https://openalex.org/keywords/enzyme-safety","display_name":"enzyme safety","score":0.7310000061988831},{"id":"https://openalex.org/keywords/acute-oral-toxicity","display_name":"acute oral toxicity","score":0.7210000157356262},{"id":"https://openalex.org/keywords/repeated-dose-toxicity","display_name":"repeated-dose toxicity","score":0.7990000247955322},{"id":"https://openalex.org/keywords/genotoxicity","display_name":"genotoxicity","score":0.9800000190734863},{"id":"https://openalex.org/keywords/mutagenicity","display_name":"mutagenicity","score":0.8289999961853027},{"id":"https://openalex.org/keywords/clastogenicity","display_name":"clastogenicity","score":0.9710000157356262},{"id":"https://openalex.org/keywords/aneugenicity","display_name":"aneugenicity","score":0.8199999928474426},{"id":"https://openalex.org/keywords/no-observed-adverse-effect-level","display_name":"no-observed-adverse-effect level","score":0.8199999928474426},{"id":"https://openalex.org/keywords/low-glycemic-index","display_name":"low glycemic index","score":0.6010000109672546},{"id":"https://openalex.org/keywords/prebiotics","display_name":"prebiotics","score":0.8980000019073486},{"id":"https://openalex.org/keywords/dietary-fiber","display_name":"dietary fiber","score":0.7020000219345093},{"id":"https://openalex.org/keywords/food-additive-safety","display_name":"food additive safety","score":0.3610000014305115}],"concepts":[{"id":"https://openalex.org/C2778959862","wikidata":"https://www.wikidata.org/wiki/Q1009245","display_name":"Genotoxicity","level":3,"score":0.7353377342224121},{"id":"https://openalex.org/C45080847","wikidata":"https://www.wikidata.org/wiki/Q1658004","display_name":"Micronucleus test","level":3,"score":0.7087575793266296},{"id":"https://openalex.org/C29730261","wikidata":"https://www.wikidata.org/wiki/Q274160","display_name":"Toxicity","level":2,"score":0.6492500901222229},{"id":"https://openalex.org/C207001950","wikidata":"https://www.wikidata.org/wiki/Q141124","display_name":"In vivo","level":2,"score":0.6275417804718018},{"id":"https://openalex.org/C141710004","wikidata":"https://www.wikidata.org/wiki/Q744364","display_name":"Clastogen","level":4,"score":0.6051158308982849},{"id":"https://openalex.org/C98274493","wikidata":"https://www.wikidata.org/wiki/Q128406","display_name":"Pharmacology","level":1,"score":0.548879861831665},{"id":"https://openalex.org/C117632636","wikidata":"https://www.wikidata.org/wiki/Q30912","display_name":"Ames test","level":4,"score":0.4608651399612427},{"id":"https://openalex.org/C116263406","wikidata":"https://www.wikidata.org/wiki/Q3299024","display_name":"Acute toxicity","level":3,"score":0.4420171082019806},{"id":"https://openalex.org/C197934379","wikidata":"https://www.wikidata.org/wiki/Q2047938","display_name":"Adverse effect","level":2,"score":0.42020294070243835},{"id":"https://openalex.org/C53164099","wikidata":"https://www.wikidata.org/wiki/Q5114007","display_name":"Chronic toxicity","level":3,"score":0.41683605313301086},{"id":"https://openalex.org/C42407357","wikidata":"https://www.wikidata.org/wiki/Q521","display_name":"Physiology","level":1,"score":0.36448174715042114},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.361160546541214},{"id":"https://openalex.org/C33070731","wikidata":"https://www.wikidata.org/wiki/Q7218","display_name":"Toxicology","level":1,"score":0.3581792116165161},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.34586048126220703},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.22487226128578186},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.16833344101905823},{"id":"https://openalex.org/C2781065037","wikidata":"https://www.wikidata.org/wiki/Q150839","display_name":"Salmonella","level":3,"score":0.11114659905433655},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.07714256644248962},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1177/23978473231215122","doi":"https://doi.org/10.1177/23978473231215122","is_oa":true,"landing_page_url":"http://dx.doi.org/10.1177/23978473231215122","pdf_url":null,"source":{"id":"https://openalex.org/S4210173702","display_name":"Toxicology Research and Application","issn_l":"2397-8473","issn":["2397-8473"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310320017","host_organization_name":"SAGE Publishing","host_organization_lineage":["https://openalex.org/P4310320017"],"host_organization_lineage_names":["SAGE Publishing"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Toxicology Research and Application","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.1177/23978473231215122","doi":"https://doi.org/10.1177/23978473231215122","is_oa":true,"landing_page_url":"http://dx.doi.org/10.1177/23978473231215122","pdf_url":null,"source":{"id":"https://openalex.org/S4210173702","display_name":"Toxicology Research and Application","issn_l":"2397-8473","issn":["2397-8473"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310320017","host_organization_name":"SAGE Publishing","host_organization_lineage":["https://openalex.org/P4310320017"],"host_organization_lineage_names":["SAGE Publishing"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Toxicology Research and Application","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.9391000270843506,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F3216757320","display_name":"Advanced Enzyme Technologies","ror":"https://ror.org/04fkxmv96"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":12,"referenced_works":["https://openalex.org/W1526605186","https://openalex.org/W1994152414","https://openalex.org/W2001094307","https://openalex.org/W2020826236","https://openalex.org/W2033248541","https://openalex.org/W2058726271","https://openalex.org/W2558829876","https://openalex.org/W2560636839","https://openalex.org/W2806143319","https://openalex.org/W3217216416","https://openalex.org/W4205802195","https://openalex.org/W4237060414"],"related_works":["https://openalex.org/W4409295740","https://openalex.org/W3121291181","https://openalex.org/W4401946881","https://openalex.org/W2086525457","https://openalex.org/W7122701357","https://openalex.org/W1997348321","https://openalex.org/W1975045729","https://openalex.org/W7165764047","https://openalex.org/W2094632170","https://openalex.org/W3015504802"],"abstract_inverted_index":{"Background":[0],"Alternansucrase":[1,79,148,220,238],"is":[2,47,65,272],"a":[3,43,66,324],"glucansucrase":[4],"enzyme":[5,68,313],"that":[6],"mainly":[7],"functions":[8],"in":[9,60,75,95,116,136,157,169,232,257,314,331],"synthesis":[10],"of":[11,20,53,163,166,191,195,251,286,311,329],"glucan-like":[12],"polysaccharides":[13,24],"and":[14,23,29,40,69,107,112,187,214,244,317],"can":[15,57],"be":[16,58,224,242,296],"used":[17,59,74],"for":[18,93,326],"production":[19],"novel":[21,67],"oligosaccharides":[22],"having":[25],"low":[26],"glycemic":[27],"index":[28],"prebiotics":[30],"properties":[31],"which":[32,279],"makes":[33],"them":[34],"ideal":[35],"candidates":[36],"as":[37,63,133,135,142],"dietary":[38],"fibers":[39],"food.":[41],"From":[42],"regulatory":[44],"perspective,":[45],"it":[46,56,64],"necessary":[48],"to":[49,86,89,189,223,227,241,247,295],"prove":[50],"the":[51,119,128,144,160,170,233,248,256,282,309],"safety":[52,92,310],"alternansucrase":[54,185,312,330],"before":[55],"any":[61,152,201],"application":[62],"has":[70],"not":[71,150,199],"been":[72],"historically":[73],"food":[76,209,316],"processing.":[77],"Objectives":[78],"obtained":[80],"from":[81],"Escherichia":[82],"coli":[83],"was":[84,221,239,293],"subjected":[85],"toxicological":[87,204],"tests":[88],"determine":[90],"its":[91],"use":[94,328],"various":[96,315],"industrial":[97,333],"applications.":[98,334],"Design":[99],"Toxicity":[100],"studies":[101,124,305],"were":[102,125],"conducted":[103,126],"at":[104,159,186],"acute":[105,171],"oral":[106,172,181],"repeated":[108,179],"sub-chronic":[109],"(14":[110],"days":[111],"90":[113],"days)":[114],"levels":[115],"rats":[117,158],"following":[118],"OECD":[120,145],"guidelines.":[121,146],"The":[122,264,304,320],"genotoxicity":[123],"using":[127],"bacterial":[129,234],"reverse":[130,235],"mutation":[131,236],"test":[132,141,249],"well":[134],"vitro":[137,258],"mammalian":[138,259],"cell":[139,260],"micronucleus":[140,261],"per":[143,302],"Results":[147],"did":[149,198],"induce":[151],"clinical":[153,215],"abnormalities":[154],"or":[155,217],"mortality":[156],"dose":[161,180,190,266],"level":[162],"2000":[164,275],"mg/kg":[165,276],"body":[167,196,207,277],"weight":[168,197],"toxicity":[173,182],"test.":[174,237,262],"A":[175],"90-day":[176],"sub":[177],"chronic":[178],"study":[183,271,322],"with":[184],"up":[188,226,246],"1000":[192,299],"mg":[193,300],"TOS/kg":[194,301],"show":[200],"treatment-related":[202],"significant":[203],"effects":[205],"on":[206,269],"weight,":[208,278],"consumption,":[210],"organ":[211],"weights,":[212],"hematological":[213],"chemistry,":[216],"histopathology":[218],"parameters.":[219],"found":[222],"non-mutagenic":[225],"5000":[228],"\u00b5g":[229,253],"TOS/plate":[230],"concentration":[231,250],"determined":[240],"non-clastogenic":[243],"non-aneugenic":[245],"1250":[252],"TOS/mL":[254],"during":[255],"Conclusion":[263],"lethal":[265],"(LD50)":[267],"based":[268],"this":[270],"greater":[273,297],"than":[274,298],"falls":[280],"into":[281],"category":[283],"5":[284],"criteria":[285],"Globally":[287],"Harmonized":[288],"System":[289],"(GHS).":[290],"No-Observed-Adverse-Effect-Level":[291],"(NOAEL)":[292],"concluded":[294],"day.":[303],"taken":[306],"together":[307],"substantiate":[308],"associated":[318],"industries.":[319],"present":[321],"paves":[323],"future":[325],"safe":[327],"varied":[332]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2024,"cited_by_count":1}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2023-11-16T00:00:00"}