{"id":"https://openalex.org/W4388588659","doi":"https://doi.org/10.1093/neuonc/noad179.1058","title":"TMET-14. PROBING TCA CYCLE ACTIVITY IN H3WT AND H3K27M-ALTERED DIFFUSE MIDLINE GLIOMAS","display_name":"TMET-14. PROBING TCA CYCLE ACTIVITY IN H3WT AND H3K27M-ALTERED DIFFUSE MIDLINE GLIOMAS","publication_year":2023,"publication_date":"2023-11-01","ids":{"openalex":"https://openalex.org/W4388588659","doi":"https://doi.org/10.1093/neuonc/noad179.1058"},"language":"en","primary_location":{"id":"doi:10.1093/neuonc/noad179.1058","doi":"https://doi.org/10.1093/neuonc/noad179.1058","is_oa":false,"landing_page_url":"https://doi.org/10.1093/neuonc/noad179.1058","pdf_url":null,"source":{"id":"https://openalex.org/S106908163","display_name":"Neuro-Oncology","issn_l":"1522-8517","issn":["1522-8517","1523-5866"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Neuro-Oncology","raw_type":"journal-article"},"volume":null,"type":"conference-abstract","indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC10639605/pdf/noad179.1058.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5037817807","display_name":"Omkar B. Ijare","orcid":"https://orcid.org/0000-0003-1855-8232","observed_orcids":["https://orcid.org/0000-0003-1855-8232"]},"institutions":[{"id":"https://openalex.org/I1295876152","display_name":"Houston Methodist","ror":"https://ror.org/027zt9171","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1295876152"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Omkar Ijare","raw_affiliation_strings":["Houston Methodist Hospital , Houston, TX , USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Houston Methodist Hospital , Houston, TX , USA","institution_ids":["https://openalex.org/I1295876152"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5093246179","display_name":"Jackson Roberson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1295876152","display_name":"Houston Methodist","ror":"https://ror.org/027zt9171","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1295876152"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jackson Roberson","raw_affiliation_strings":["Houston Methodist , Houston, TX , USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Houston Methodist , Houston, TX , USA","institution_ids":["https://openalex.org/I1295876152"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101266113","display_name":"Sushmita Kumar","orcid":"https://orcid.org/0009-0006-8072-7024","observed_orcids":["https://orcid.org/0009-0006-8072-7024"]},"institutions":[{"id":"https://openalex.org/I1295876152","display_name":"Houston Methodist","ror":"https://ror.org/027zt9171","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1295876152"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Sushmita Kumar","raw_affiliation_strings":["LAWRENCE E. ELKINS HIGH SCHOOL/Houston Methodist Hospital , Houston, TX , USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"LAWRENCE E. ELKINS HIGH SCHOOL/Houston Methodist Hospital , Houston, TX , USA","institution_ids":["https://openalex.org/I1295876152"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111072868","display_name":"Rishith Ramamurthy","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1295876152","display_name":"Houston Methodist","ror":"https://ror.org/027zt9171","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1295876152"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Rishith Ramamurthy","raw_affiliation_strings":["Houston Methodist , Houston, TX , USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Houston Methodist , Houston, TX , USA","institution_ids":["https://openalex.org/I1295876152"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113572501","display_name":"David S. Baskin","orcid":"https://orcid.org/0000-0002-8076-382X","observed_orcids":["https://orcid.org/0000-0002-8076-382X"]},"institutions":[{"id":"https://openalex.org/I1295876152","display_name":"Houston Methodist","ror":"https://ror.org/027zt9171","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1295876152"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"David S Baskin","raw_affiliation_strings":["Houston Methodist Hospital , Houston , USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Houston Methodist Hospital , Houston , USA","institution_ids":["https://openalex.org/I1295876152"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5036568543","display_name":"Kumar Pichumani","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1295876152","display_name":"Houston Methodist","ror":"https://ror.org/027zt9171","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1295876152"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kumar Pichumani","raw_affiliation_strings":["Houston Methodist Hospital , Houston, TX , USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Houston Methodist Hospital , Houston, TX , USA","institution_ids":["https://openalex.org/I1295876152"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I1295876152"],"apc_list":{"value":4612,"currency":"USD","value_usd":4612},"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"25","issue":"Supplement_5","first_page":"v275","last_page":"v275"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10631","display_name":"Cancer, Hypoxia, and Metabolism","score":0.8389000296592712,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10631","display_name":"Cancer, Hypoxia, and Metabolism","score":0.8389000296592712,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10129","display_name":"Glioma Diagnosis and Treatment","score":0.12099999934434891,"subfield":{"id":"https://openalex.org/subfields/2716","display_name":"Genetics (clinical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10836","display_name":"Metabolomics and Mass Spectrometry Studies","score":0.004000000189989805,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/diffuse-midline-glioma","display_name":"diffuse midline glioma","score":0.8899999856948853},{"id":"https://openalex.org/keywords/h3k27m-mutation","display_name":"H3K27M mutation","score":0.8849999904632568},{"id":"https://openalex.org/keywords/alpha-ketoglutarate","display_name":"alpha-ketoglutarate","score":0.8610000014305115},{"id":"https://openalex.org/keywords/tricarboxylic-acid-cycle","display_name":"TCA cycle","score":0.8529999852180481},{"id":"https://openalex.org/keywords/glutamine-metabolism","display_name":"glutamine metabolism","score":0.7599999904632568},{"id":"https://openalex.org/keywords/glucose-metabolism","display_name":"glucose metabolism","score":0.9869999885559082},{"id":"https://openalex.org/keywords/reductive-carboxylation","display_name":"reductive carboxylation","score":0.8289999961853027},{"id":"https://openalex.org/keywords/citrate","display_name":"citrate","score":0.7110000252723694},{"id":"https://openalex.org/keywords/pyruvate","display_name":"pyruvate","score":0.7950000166893005},{"id":"https://openalex.org/keywords/lactate","display_name":"lactate","score":0.9729999899864197},{"id":"https://openalex.org/keywords/mitochondrial-metabolism","display_name":"mitochondrial metabolism","score":0.6470000147819519},{"id":"https://openalex.org/keywords/metabolic-flux-analysis","display_name":"metabolic flux analysis","score":0.765999972820282},{"id":"https://openalex.org/keywords/13c-isotopomer-analysis","display_name":"13C isotopomer analysis","score":0.7289999723434448},{"id":"https://openalex.org/keywords/neurospheres","display_name":"neurospheres","score":0.7720000147819519}],"concepts":[{"id":"https://openalex.org/C2779349466","wikidata":"https://www.wikidata.org/wiki/Q181619","display_name":"Glutamine","level":3,"score":0.9042161703109741},{"id":"https://openalex.org/C165135838","wikidata":"https://www.wikidata.org/wiki/Q133895","display_name":"Citric acid cycle","level":3,"score":0.8053998947143555},{"id":"https://openalex.org/C62231903","wikidata":"https://www.wikidata.org/wiki/Q1057","display_name":"Metabolism","level":2,"score":0.6205813884735107},{"id":"https://openalex.org/C2776441662","wikidata":"https://www.wikidata.org/wiki/Q2823314","display_name":"Tricarboxylic acid","level":4,"score":0.552233874797821},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5081641674041748},{"id":"https://openalex.org/C29537977","wikidata":"https://www.wikidata.org/wiki/Q188941","display_name":"Cell cycle","level":3,"score":0.49658042192459106},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.39250171184539795},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.32820767164230347},{"id":"https://openalex.org/C190283241","wikidata":"https://www.wikidata.org/wiki/Q14599311","display_name":"Apoptosis","level":2,"score":0.23684772849082947},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.20910733938217163}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1093/neuonc/noad179.1058","doi":"https://doi.org/10.1093/neuonc/noad179.1058","is_oa":false,"landing_page_url":"https://doi.org/10.1093/neuonc/noad179.1058","pdf_url":null,"source":{"id":"https://openalex.org/S106908163","display_name":"Neuro-Oncology","issn_l":"1522-8517","issn":["1522-8517","1523-5866"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Neuro-Oncology","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:10639605","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/10639605","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC10639605/pdf/noad179.1058.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Neuro Oncol","raw_type":"Text"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:10639605","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/10639605","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC10639605/pdf/noad179.1058.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Neuro Oncol","raw_type":"Text"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.8999999761581421}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4388588659.pdf","grobid_xml":"https://content.openalex.org/works/W4388588659.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4416140013","https://openalex.org/W3112184199","https://openalex.org/W3110163092","https://openalex.org/W4310093498","https://openalex.org/W3216200822","https://openalex.org/W3048798848","https://openalex.org/W4283033179","https://openalex.org/W7128586902","https://openalex.org/W7005612227","https://openalex.org/W2332504145"],"abstract_inverted_index":{"Abstract":[0],"DMG":[1,20,51,79,175,204,236,297],"has":[2],"high":[3],"levels":[4,58,120,221],"of":[5,24,39,55,121,174,185,203,212,222,281,289,295],"\u03b1-ketoglutarate":[6],"(\u03b1-KG),":[7],"causing":[8],"histone":[9],"hypomethylation":[10],"and":[11,44,71,76,131],"promoting":[12],"tumor":[13,97],"growth.":[14],"Therefore,":[15,278],"targeting":[16],"\u03b1-KG":[17,27,57,213,225,282],"production":[18],"in":[19,30,50,74,96,152,158,199,256,283,292],"tumors":[21],"may":[22],"be":[23,288],"therapeutic":[25,290],"value.":[26],"is":[28,208],"synthesized":[29],"the":[31,53,144,147,153,183,257,293],"tricarboxylic":[32],"acid":[33],"cycle":[34,36,155,179,259],"(TCA)":[35],"via":[37,240,252],"oxidation":[38],"multiple":[40],"nutrients":[41],"including":[42],"glucose":[43,70,148],"glutamine.":[45],"Hence,":[46],"probing":[47],"mitochondrial":[48],"metabolism":[49,73,198,255],"with":[52,103],"goal":[54],"altering":[56],"will":[59],"lead":[60],"to":[61,64,143,182,248],"new":[62],"therapies":[63],"treat":[65],"DMG.":[66],"Here,":[67],"we":[68,188,195],"examine":[69],"glutamine":[72,207,254],"H3WT":[75],"H3K27M-altered":[77,116,235],"patient-derived":[78],"cells":[80,91,117,205],"(Dr.":[81],"Monje\u2019s":[82],"lab)":[83],"using":[84],"GC-MS":[85],"based":[86],"metabolic":[87],"flux":[88,239],"analysis.":[89],"Both":[90,216],"were":[92,101],"grown":[93],"as":[94,206],"neurospheres":[95],"stem":[98],"medium.":[99],"Neurospheres":[100],"treated":[102],"11.0":[104],"mM":[105,109],"[U-13C]glucose":[106],"or":[107],"4.0":[108],"[U-13C]glutamine.":[110],"The":[111],"13C-isotopomer":[112],"analysis":[113],"revealed":[114],"that":[115,171],"produced":[118,251],"higher":[119,220,238],"(M+3)":[122,132],"pyruvate":[123],"(14.05":[124],"\u00b1":[125,129,135,139,162,166,227,232,263,267,272,276],"5.37%":[126],"vs.":[127,164,230,265,274],"7.15":[128],"2.98%),":[130],"lactate":[133],"(9.46":[134],"6.48":[136],"%":[137,229],"vs.3.87":[138],"1.80":[140],"%)":[141],"compared":[142,247],"H3WT.":[145],"Whereas,":[146],"derived":[149],"(M+2)":[150],"citrate":[151,245,250,269],"TCA":[154,178,258],"was":[156],"similar":[157,177],"both":[159,172,296],"cases":[160],"(15.81":[161],"2.91%":[163],"16.76":[165],"4.15%).":[167],"This":[168],"data":[169],"shows":[170],"types":[173,202],"possess":[176],"activity":[180],"leading":[181],"synthesis":[184,214,280],"\u03b1-KG,":[186],"which":[187],"measured":[189],"through":[190],"glutamate":[191],"13C":[192],"labeling.":[193],"Next,":[194],"probed":[196],"[U-13C]glutamine":[197],"these":[200],"two":[201],"another":[209],"major":[210],"source":[211],".":[215],"cell":[217],"lines":[218],"showed":[219,237],"glutamine-derived":[223],"M+5":[224,244],"(34.62":[226],"2.12":[228],"42.17":[231],"5.05%).":[233],"Interestingly,":[234],"reductive":[241],"carboxylation":[242],"generating":[243],"when":[246],"M+4":[249],"canonical":[253],"(citrate":[260],"M+5:":[261],"7.13":[262],"2.14%":[264],"4.33":[266],"1.68%;":[268],"M+4:":[270],"4.04":[271],"1.06%":[273],"7.85":[275],"1.52%).":[277],"inhibiting":[279],"H3":[284],"K27M-altered":[285],"DMGs":[286],"would":[287],"interest":[291],"treatment":[294],"subtypes.":[298]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2023-11-12T00:00:00"}