{"id":"https://openalex.org/W4388428517","doi":"https://doi.org/10.21769/bioprotoc.4873","title":"Identification of Acetylation Sites of Fatty Acid Synthase (FASN) by Mass Spectrometry and FASN Activity Assay","display_name":"Identification of Acetylation Sites of Fatty Acid Synthase (FASN) by Mass Spectrometry and FASN Activity Assay","publication_year":2023,"publication_date":"2023-01-01","ids":{"openalex":"https://openalex.org/W4388428517","doi":"https://doi.org/10.21769/bioprotoc.4873","pmid":"https://pubmed.ncbi.nlm.nih.gov/37969759","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/10632157"},"language":"en","primary_location":{"id":"doi:10.21769/bioprotoc.4873","doi":"https://doi.org/10.21769/bioprotoc.4873","is_oa":true,"landing_page_url":"https://doi.org/10.21769/bioprotoc.4873","pdf_url":null,"source":{"id":"https://openalex.org/S4210179406","display_name":"BIO-PROTOCOL","issn_l":"2331-8325","issn":["2331-8325"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","norway-1","oarl","poland-20","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320252","host_organization_name":"American Academy of Arts and Sciences","host_organization_lineage":["https://openalex.org/P4310320252"],"host_organization_lineage_names":["American Academy of Arts and Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BIO-PROTOCOL","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://doi.org/10.21769/bioprotoc.4873","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5056571397","display_name":"Ting Miao","orcid":"https://orcid.org/0000-0002-0634-7377","observed_orcids":["https://orcid.org/0000-0002-0634-7377"]},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Ting Miao","raw_affiliation_strings":["Department of Genetics, Harvard Medical School, Boston, MA 02115, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Genetics, Harvard Medical School, Boston, MA 02115, USA","institution_ids":["https://openalex.org/I136199984"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5087680483","display_name":"Hua Bai","orcid":"https://orcid.org/0000-0003-2221-7545","observed_orcids":["https://orcid.org/0000-0003-2221-7545","https://orcid.org/0000-0002-5436-1245"]},"institutions":[{"id":"https://openalex.org/I173911158","display_name":"Iowa State University","ror":"https://ror.org/04rswrd78","country_code":"US","type":"education","lineage":["https://openalex.org/I173911158"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Hua Bai","raw_affiliation_strings":["Department of Genetics, Development, and Cell Biology, Iowa State University, Ames, IA, 50011, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Genetics, Development, and Cell Biology, Iowa State University, Ames, IA, 50011, USA","institution_ids":["https://openalex.org/I173911158"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5056571397","https://openalex.org/A5087680483"],"corresponding_institution_ids":["https://openalex.org/I136199984","https://openalex.org/I173911158"],"apc_list":null,"apc_paid":null,"fwci":0.196,"has_fulltext":true,"cited_by_count":2,"citation_normalized_percentile":{"value":0.43626898,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":95,"max":97},"biblio":{"volume":"13","issue":"21","first_page":"e4873","last_page":"e4873"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12465","display_name":"Cancer, Lipids, and Metabolism","score":0.7387999892234802,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12465","display_name":"Cancer, Lipids, and Metabolism","score":0.7387999892234802,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.11789999902248383,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11844","display_name":"Lipid metabolism and biosynthesis","score":0.0949999988079071,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/fatty-acid-synthase","display_name":"fatty acid synthase","score":0.9959999918937683},{"id":"https://openalex.org/keywords/lysine-acetylation","display_name":"lysine acetylation","score":0.9959999918937683},{"id":"https://openalex.org/keywords/de-novo-lipogenesis","display_name":"de novo lipogenesis","score":0.7870000004768372},{"id":"https://openalex.org/keywords/immunoprecipitation","display_name":"immunoprecipitation","score":0.8199999928474426},{"id":"https://openalex.org/keywords/sample-preparation","display_name":"sample preparation","score":0.6769999861717224},{"id":"https://openalex.org/keywords/post-translational-modification","display_name":"post-translational modification","score":0.8180000185966492},{"id":"https://openalex.org/keywords/drosophila-larvae","display_name":"Drosophila larvae","score":0.8090000152587891}],"concepts":[{"id":"https://openalex.org/C119157956","wikidata":"https://www.wikidata.org/wiki/Q796604","display_name":"Acetylation","level":3,"score":0.8301140069961548},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.65067058801651},{"id":"https://openalex.org/C141359234","wikidata":"https://www.wikidata.org/wiki/Q2734301","display_name":"Lipogenesis","level":3,"score":0.6425564289093018},{"id":"https://openalex.org/C48289651","wikidata":"https://www.wikidata.org/wiki/Q106916817","display_name":"Fatty acid synthase","level":3,"score":0.6402789950370789},{"id":"https://openalex.org/C2776016237","wikidata":"https://www.wikidata.org/wiki/Q178430","display_name":"Lysine","level":3,"score":0.564848005771637},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5100013017654419},{"id":"https://openalex.org/C46111723","wikidata":"https://www.wikidata.org/wiki/Q471857","display_name":"Proteomics","level":3,"score":0.49244317412376404},{"id":"https://openalex.org/C64927066","wikidata":"https://www.wikidata.org/wiki/Q36293","display_name":"Histone","level":3,"score":0.46728190779685974},{"id":"https://openalex.org/C2777989768","wikidata":"https://www.wikidata.org/wiki/Q28747","display_name":"Nicotinamide adenine dinucleotide phosphate","level":4,"score":0.41732388734817505},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.37052005529403687},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.31095588207244873},{"id":"https://openalex.org/C4733338","wikidata":"https://www.wikidata.org/wiki/Q2792936","display_name":"Lipid metabolism","level":2,"score":0.20073175430297852},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.1781841218471527},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.08412346243858337},{"id":"https://openalex.org/C38485361","wikidata":"https://www.wikidata.org/wiki/Q576379","display_name":"Oxidase test","level":3,"score":0.0}],"mesh":[],"locations_count":5,"locations":[{"id":"doi:10.21769/bioprotoc.4873","doi":"https://doi.org/10.21769/bioprotoc.4873","is_oa":true,"landing_page_url":"https://doi.org/10.21769/bioprotoc.4873","pdf_url":null,"source":{"id":"https://openalex.org/S4210179406","display_name":"BIO-PROTOCOL","issn_l":"2331-8325","issn":["2331-8325"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","norway-1","oarl","poland-20","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320252","host_organization_name":"American Academy of Arts and Sciences","host_organization_lineage":["https://openalex.org/P4310320252"],"host_organization_lineage_names":["American Academy of Arts and Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BIO-PROTOCOL","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:10632157","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/10632157","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC10632157/pdf/BioProtoc-13-21-4873.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bio Protoc","raw_type":"Text"},{"id":"pmid:37969759","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/37969759","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bio-protocol","raw_type":"Journal Article"},{"id":"pmh:oai:dr.lib.iastate.edu:20.500.12876/nrQB6K9z","doi":null,"is_oa":true,"landing_page_url":"https://dr.lib.iastate.edu/handle/20.500.12876/nrQB6K9z","pdf_url":null,"source":{"id":"https://openalex.org/S4377196104","display_name":"Iowa State University Digital Repository (Iowa State University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I173911158","host_organization_name":"Iowa State University","host_organization_lineage":["https://openalex.org/I173911158"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"https://doi.org/10.21769/BioProtoc.4873","raw_type":"Article"},{"id":"pmh:oai:doaj.org/article:a9146c0734c74c1cb3f6af916f52ec68","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/a9146c0734c74c1cb3f6af916f52ec68","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Bio-Protocol, Vol 13, Iss 21 (2023)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.21769/bioprotoc.4873","doi":"https://doi.org/10.21769/bioprotoc.4873","is_oa":true,"landing_page_url":"https://doi.org/10.21769/bioprotoc.4873","pdf_url":null,"source":{"id":"https://openalex.org/S4210179406","display_name":"BIO-PROTOCOL","issn_l":"2331-8325","issn":["2331-8325"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","norway-1","oarl","poland-20","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320252","host_organization_name":"American Academy of Arts and Sciences","host_organization_lineage":["https://openalex.org/P4310320252"],"host_organization_lineage_names":["American Academy of Arts and Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BIO-PROTOCOL","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2607579963","display_name":"Autonomous and Non-Autonomous Regulation of Cardiac Aging","funder_award_id":"R01AG058741","funder_id":"https://openalex.org/F4320337337","funder_display_name":"National Institute on Aging"},{"id":"https://openalex.org/G5890517345","display_name":"CAREER: Understanding Peroxisomal Stress Responses","funder_award_id":"2046984","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"},{"id":"https://openalex.org/G6671297155","display_name":null,"funder_award_id":"CAREER","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"}],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337337","display_name":"National Institute on Aging","ror":"https://ror.org/049v75w11"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4388428517.pdf","grobid_xml":"https://content.openalex.org/works/W4388428517.grobid-xml"},"referenced_works_count":20,"referenced_works":["https://openalex.org/W1506966309","https://openalex.org/W1540264737","https://openalex.org/W1867114068","https://openalex.org/W1963507142","https://openalex.org/W2009826707","https://openalex.org/W2011540184","https://openalex.org/W2043274355","https://openalex.org/W2048469489","https://openalex.org/W2054085725","https://openalex.org/W2064921878","https://openalex.org/W2087714970","https://openalex.org/W2168921982","https://openalex.org/W2544360569","https://openalex.org/W2610582292","https://openalex.org/W2743588819","https://openalex.org/W2892416438","https://openalex.org/W3016525483","https://openalex.org/W3030580393","https://openalex.org/W3118894576","https://openalex.org/W4310780843"],"related_works":["https://openalex.org/W4206050598","https://openalex.org/W4310780843","https://openalex.org/W2054499954","https://openalex.org/W3000713644","https://openalex.org/W3157486080","https://openalex.org/W2091037376","https://openalex.org/W4299325328","https://openalex.org/W2946543077","https://openalex.org/W4200264640","https://openalex.org/W2766171218"],"abstract_inverted_index":{"Lysine":[0],"acetylation":[1,65,110,162],"is":[2,138],"a":[3,8,99,123],"conserved":[4],"post-translational":[5],"modification":[6],"and":[7,21,41,106,158],"key":[9],"regulatory":[10],"mechanism":[11],"for":[12,109,125,133,155,161,171],"various":[13,89],"cellular":[14,22],"processes,":[15],"including":[16],"metabolic":[17],"control,":[18],"epigenetic":[19],"regulation,":[20],"signaling":[23],"transduction.":[24],"Recent":[25],"advances":[26],"in":[27,88],"mass":[28,166],"spectrometry":[29],"(MS)":[30],"enable":[31],"the":[32,55,75,141],"extensive":[33],"identification":[34,111,164],"of":[35,39,57,79,102,112,140,145,173,176],"acetylated":[36,87],"lysine":[37],"residues":[38],"histone":[40],"non-histone":[42],"proteins.":[43],"However,":[44],"protein":[45,104,115,156],"enrichment":[46,105],"before":[47],"MS":[48],"analysis":[49],"may":[50],"be":[51,86],"necessary":[52],"to":[53,85,94],"improve":[54],"detection":[56],"low-abundant":[58],"proteins":[59,61],"or":[60],"that":[62],"exhibit":[63],"low":[64],"levels.":[66],"Fatty":[67],"acid":[68],"synthase":[69],"(FASN),":[70],"an":[71,181],"essential":[72],"enzyme":[73],"catalyzing":[74],"de":[76,146],"novo":[77,147],"synthesis":[78],"fatty":[80],"acids,":[81],"has":[82],"been":[83],"found":[84],"species,":[90],"from":[91],"fruit":[92,177],"flies":[93],"humans.":[95],"Here,":[96],"we":[97,121],"describe":[98],"step-by-step":[100],"process":[101],"antibody-based":[103],"sample":[107,159],"preparation":[108,160],"endogenous":[113],"FASN":[114,134,174],"by":[116,165],"MS-based":[117],"proteomics":[118],"analysis.":[119],"Meanwhile,":[120],"provide":[122],"protocol":[124,154],"nicotinamide":[126],"adenine":[127],"dinucleotide":[128],"phosphate":[129],"(NADPH)":[130],"absorbance":[131,182],"assay":[132],"activity":[135,175],"measurement,":[136],"which":[137],"one":[139],"primary":[142],"functional":[143],"readouts":[144],"lipogenesis.":[148],"Key":[149],"features":[150],"\u2022":[151,168],"A":[152],"comprehensive":[153],"immunoprecipitation":[157],"site":[163],"spectrometry.":[167],"Step-by-step":[169],"procedures":[170],"measurement":[172],"fly":[178],"larvae":[179],"using":[180],"assay.":[183]},"counts_by_year":[{"year":2026,"cited_by_count":2}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2023-11-07T00:00:00"}
