{"id":"https://openalex.org/W4388360767","doi":"https://doi.org/10.1093/oso/9780195088717.003.0015","title":"Special Considerations Encountered with Thick Specimens","display_name":"Special Considerations Encountered with Thick Specimens","publication_year":2007,"publication_date":"2007-06-08","ids":{"openalex":"https://openalex.org/W4388360767","doi":"https://doi.org/10.1093/oso/9780195088717.003.0015"},"language":"en","primary_location":{"id":"doi:10.1093/oso/9780195088717.003.0015","doi":"https://doi.org/10.1093/oso/9780195088717.003.0015","is_oa":false,"landing_page_url":"http://dx.doi.org/10.1093/oso/9780195088717.003.0015","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Electron Crystallography Of Biological Macromolecules","raw_type":"book-chapter"},"volume":{"id":"https://openalex.org/W4388331828","display_name":"Electron Crystallography Of Biological Macromolecules"},"type":"book-chapter","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5045999939","display_name":"Robert M. Glaeser","orcid":"https://orcid.org/0000-0003-1955-1825","observed_orcids":["https://orcid.org/0000-0003-1955-1825"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Robert M Glaeser","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5023739289","display_name":"Kenneth H. Downing","orcid":"https://orcid.org/0000-0002-3543-7013","observed_orcids":["https://orcid.org/0000-0002-3543-7013"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Kenneth Downing","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5081649301","display_name":"David J. DeRosier","orcid":"https://orcid.org/0000-0001-7946-3371","observed_orcids":["https://orcid.org/0000-0001-7946-3371"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"David DeRosier","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5049689333","display_name":"Wah Chiu","orcid":"https://orcid.org/0000-0002-8910-3078","observed_orcids":["https://orcid.org/0000-0002-8910-3078"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Wah Chiu","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5042892654","display_name":"Joachim Frank","orcid":"https://orcid.org/0000-0001-5449-6943","observed_orcids":["https://orcid.org/0000-0001-5449-6943","https://orcid.org/0000-0002-2442-1112"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Joachim Frank","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.40310078,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":"415","last_page":"440"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10857","display_name":"Advanced Electron Microscopy Techniques and Applications","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10857","display_name":"Advanced Electron Microscopy Techniques and Applications","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12039","display_name":"Electron and X-Ray Spectroscopy Techniques","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2508","display_name":"Surfaces, Coatings and Films"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/thick-specimens","display_name":"thick samples","score":0.75},{"id":"https://openalex.org/keywords/electron-diffraction","display_name":"electron diffraction","score":0.6869999766349792},{"id":"https://openalex.org/keywords/biological-macromolecules","display_name":"biological macromolecules","score":0.921999990940094},{"id":"https://openalex.org/keywords/elastic-scattering","display_name":"elastic scattering","score":0.5540000200271606},{"id":"https://openalex.org/keywords/inelastic-scattering","display_name":"inelastic scattering","score":0.8930000066757202},{"id":"https://openalex.org/keywords/specimen-thickness-effect","display_name":"specimen thickness effect","score":0.5550000071525574}],"concepts":[{"id":"https://openalex.org/C195065555","wikidata":"https://www.wikidata.org/wiki/Q214881","display_name":"Curvature","level":2,"score":0.7239171266555786},{"id":"https://openalex.org/C191486275","wikidata":"https://www.wikidata.org/wiki/Q210028","display_name":"Scattering","level":2,"score":0.6413065195083618},{"id":"https://openalex.org/C147120987","wikidata":"https://www.wikidata.org/wiki/Q2225","display_name":"Electron","level":2,"score":0.5957293510437012},{"id":"https://openalex.org/C142199849","wikidata":"https://www.wikidata.org/wiki/Q3027672","display_name":"Inelastic scattering","level":3,"score":0.48429766297340393},{"id":"https://openalex.org/C12426560","wikidata":"https://www.wikidata.org/wiki/Q189569","display_name":"Basis (linear algebra)","level":2,"score":0.4590742290019989},{"id":"https://openalex.org/C146285849","wikidata":"https://www.wikidata.org/wiki/Q1327107","display_name":"Electron scattering","level":3,"score":0.4115142822265625},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.3901601731777191},{"id":"https://openalex.org/C30475298","wikidata":"https://www.wikidata.org/wiki/Q909554","display_name":"Computational physics","level":1,"score":0.3809276223182678},{"id":"https://openalex.org/C74650414","wikidata":"https://www.wikidata.org/wiki/Q11397","display_name":"Classical mechanics","level":1,"score":0.3768083155155182},{"id":"https://openalex.org/C33332235","wikidata":"https://www.wikidata.org/wiki/Q18362","display_name":"Theoretical physics","level":1,"score":0.36136916279792786},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.33592331409454346},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.3314353823661804},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.2567695677280426},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.18328934907913208},{"id":"https://openalex.org/C2524010","wikidata":"https://www.wikidata.org/wiki/Q8087","display_name":"Geometry","level":1,"score":0.15221047401428223}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1093/oso/9780195088717.003.0015","doi":"https://doi.org/10.1093/oso/9780195088717.003.0015","is_oa":false,"landing_page_url":"http://dx.doi.org/10.1093/oso/9780195088717.003.0015","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Electron Crystallography Of Biological Macromolecules","raw_type":"book-chapter"}],"best_oa_location":null,"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W55883722","https://openalex.org/W2394379345","https://openalex.org/W3215763795","https://openalex.org/W2079726820","https://openalex.org/W4288816678","https://openalex.org/W1984937150","https://openalex.org/W2045575369","https://openalex.org/W2023821842","https://openalex.org/W2030848389","https://openalex.org/W2024949328"],"abstract_inverted_index":{"Abstract":[0],"A":[1],"number":[2],"of":[3,9,12,19,29,46],"idealized":[4],"assumptions":[5,31],"formed":[6],"the":[7,10,23,47,76],"basis":[8],"majority":[11],"what":[13],"was":[14],"said":[15],"about":[16],"electron":[17],"microscopy":[18],"biological":[20],"macromolecules":[21],"in":[22],"previous":[24],"chapters.":[25],"The":[26],"most":[27],"important":[28],"these":[30,61],"were:":[32],"(1)":[33],"elastically":[34],"scattered":[35],"electrons":[36],"undergo":[37],"no":[38],"more":[39],"than":[40],"one":[41,70],"scattering":[42,56],"event,":[43],"(2)":[44],"curvature":[45],"Ewald":[48],"sphere":[49],"can":[50,57],"be":[51,58],"neglected,":[52],"and":[53],"(3)":[54],"inelastic":[55],"ignored.":[59],"While":[60],"represent":[62],"satisfactory":[63],"approximations":[64],"for":[65],"suitably":[66],"thin":[67],"specimens,":[68],"each":[69],"becomes":[71],"progressively":[72],"less":[73],"good":[74],"as":[75],"specimen":[77],"thickness":[78],"increases.":[79]},"counts_by_year":[],"updated_date":"2026-10-17T09:40:24.721904","created_date":"2025-10-10T00:00:00"}