{"id":"https://openalex.org/W4388212565","doi":null,"title":"Effect of Extracellular Matrix on Differentiation of Mouse Embryonic Stem Cells into Insulin-Producing Cells","display_name":"Effect of Extracellular Matrix on Differentiation of Mouse Embryonic Stem Cells into Insulin-Producing Cells","publication_year":2009,"publication_date":"2009-01-01","ids":{"openalex":"https://openalex.org/W4388212565"},"language":"en","primary_location":{"id":"pmh:oai:doaj.org/article:6f4de29988a94c6d87c77c9be9882bc2","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/6f4de29988a94c6d87c77c9be9882bc2","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Cell Journal, Vol 10, Iss 4, Pp 250-259 (2009)","raw_type":"article"},"volume":null,"type":"article","indexed_in":["doaj"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doaj.org/article/6f4de29988a94c6d87c77c9be9882bc2","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5076737665","display_name":"Ali Farrokhi","orcid":"https://orcid.org/0000-0002-6345-5585","observed_orcids":["https://orcid.org/0000-0002-6345-5585"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"A. Farrokhi","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5006967737","display_name":"Elahe Elahi","orcid":"https://orcid.org/0000-0002-6897-2223","observed_orcids":["https://orcid.org/0000-0002-6897-2223"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"E. Elahi","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015868273","display_name":"Mehdi Pirouz","orcid":"https://orcid.org/0000-0002-7439-6066","observed_orcids":["https://orcid.org/0000-0002-7439-6066"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"M.Pirouz","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5050132319","display_name":"Hanieh Jafary","orcid":"https://orcid.org/0000-0002-0958-9072","observed_orcids":["https://orcid.org/0000-0002-0958-9072"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"H. Jafary","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5091186278","display_name":"Hossein Baharvand","orcid":"https://orcid.org/0000-0001-6528-3687","observed_orcids":["https://orcid.org/0000-0001-6528-3687"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"H. Baharvand","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":{"value":0.58183075,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10839","display_name":"Pancreatic function and diabetes","score":0.9955999851226807,"subfield":{"id":"https://openalex.org/subfields/2746","display_name":"Surgery"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10839","display_name":"Pancreatic function and diabetes","score":0.9955999851226807,"subfield":{"id":"https://openalex.org/subfields/2746","display_name":"Surgery"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":0.00430000014603138,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12264","display_name":"Tissue Engineering and Regenerative Medicine","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2746","display_name":"Surgery"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/extracellular-matrix","display_name":"extracellular matrix","score":0.9850000143051147},{"id":"https://openalex.org/keywords/mouse-embryonic-stem-cells","display_name":"mouse embryonic stem cells","score":0.9559999704360962},{"id":"https://openalex.org/keywords/pancreatic-beta-cell-differentiation","display_name":"pancreatic beta cell differentiation","score":0.7689999938011169},{"id":"https://openalex.org/keywords/insulin-secretion","display_name":"insulin secretion","score":0.6819999814033508},{"id":"https://openalex.org/keywords/matrigel","display_name":"Matrigel","score":0.9160000085830688},{"id":"https://openalex.org/keywords/gelatin","display_name":"gelatin","score":0.9570000171661377},{"id":"https://openalex.org/keywords/embryoid-bodies","display_name":"embryoid bodies","score":0.8299999833106995},{"id":"https://openalex.org/keywords/nicotinamide","display_name":"nicotinamide","score":0.8420000076293945},{"id":"https://openalex.org/keywords/c-peptide","display_name":"C-peptide","score":0.656000018119812},{"id":"https://openalex.org/keywords/glucose-stimulated-insulin-secretion","display_name":"glucose-stimulated insulin secretion","score":0.7250000238418579}],"concepts":[{"id":"https://openalex.org/C145103041","wikidata":"https://www.wikidata.org/wiki/Q1151519","display_name":"Embryonic stem cell","level":3,"score":0.7326986789703369},{"id":"https://openalex.org/C189165786","wikidata":"https://www.wikidata.org/wiki/Q193825","display_name":"Extracellular matrix","level":2,"score":0.7188135981559753},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.6850032210350037},{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.6026237607002258},{"id":"https://openalex.org/C106487976","wikidata":"https://www.wikidata.org/wiki/Q685816","display_name":"Matrix (chemical analysis)","level":2,"score":0.45397308468818665},{"id":"https://openalex.org/C148738053","wikidata":"https://www.wikidata.org/wiki/Q210861","display_name":"Cellular differentiation","level":3,"score":0.43089744448661804},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.42765042185783386},{"id":"https://openalex.org/C2779306644","wikidata":"https://www.wikidata.org/wiki/Q2002370","display_name":"Insulin","level":2,"score":0.4142061173915863},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.39632073044776917},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.20547348260879517},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.15363162755966187},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.07838517427444458},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"pmh:oai:doaj.org/article:6f4de29988a94c6d87c77c9be9882bc2","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/6f4de29988a94c6d87c77c9be9882bc2","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Cell Journal, Vol 10, Iss 4, Pp 250-259 (2009)","raw_type":"article"}],"best_oa_location":{"id":"pmh:oai:doaj.org/article:6f4de29988a94c6d87c77c9be9882bc2","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/6f4de29988a94c6d87c77c9be9882bc2","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Cell Journal, Vol 10, Iss 4, Pp 250-259 (2009)","raw_type":"article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.6240000128746033}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1521943995","https://openalex.org/W2019852895","https://openalex.org/W7219582022","https://openalex.org/W2460868197","https://openalex.org/W2303083848","https://openalex.org/W4313312939","https://openalex.org/W7033596705","https://openalex.org/W2977615722","https://openalex.org/W2078461891","https://openalex.org/W2110459242"],"abstract_inverted_index":{"Objective:":[0],"Evaluation":[1],"of":[2,34,70,79,91,94,108,118,134,174,191],"extracellular":[3],"matrics":[4],"(ECMs)":[5],"effect":[6],"on":[7,111,153],"differentiation":[8,38,192],"ofembryonic":[9],"stem":[10],"cells":[11,28,85,110,152,181],"(ESCs)":[12],"to":[13,25,98,129,165],"pancreatic":[14],"\u03b2-cell.Materials":[15],"and":[16,39,49,56,81,87,145,182],"Methods:":[17],"Mouse":[18],"ESC":[19,178],"line,":[20],"Royan":[21],"B1,":[22],"was":[23,185],"subjected":[24],"differentiationinto":[26,179],"\u03b2-like":[27],"in":[29,84,127,160,163],"a":[30],"three-step":[31],"method:":[32],"generation":[33],"embryoid":[35],"bodies(EBs),":[36],"spontaneous":[37],"induction":[40],"by":[41],"Nicotinamide":[42],"onto":[43],"differentmatrices":[44],"including":[45,121],"poly":[46],"L-ornithine/laminin,":[47],"gelatin,":[48],"two":[50],"different":[51],"dilution":[52],"ofmatrigel":[53],"(1:30,":[54],"1:100)":[55],"control":[57],"group":[58],"(no":[59],"ECM).":[60],"At":[61],"the":[62,89,92,130,166],"final":[63],"step,":[64],"differentiatedcells":[65],"were":[66],"analyzed":[67],"for":[68,77,88,136],"expression":[69,117],"some":[71],"pancreas-specific":[72],"genes":[73],"using":[74,196],"\"semiquantitativeRT-PCR":[75],"\",":[76],"detection":[78],"insulin":[80,147,159],"C-peptide":[82,137],"presence":[83],"using\"immunocytochemistry\"":[86],"evaluation":[90],"amount":[93],"secretd":[95],"insulinin":[96],"response":[97],"glucose":[99,162],"Using":[100],"\"insulin":[101],"secretion":[102,148],"assay\".Results:":[103],"The":[104],"semi-quantitative":[105],"RT-PCR":[106],"analysis":[107],"differentiated":[109],"1:30matrigel":[112],"coated-plates":[113,156],"showed":[114,138],"consistent":[115],"higher":[116],"\u03b2-cell":[119],"specificmarkers":[120],"Insulin":[122,124],"I,":[123],"II,":[125],"Slc2a2":[126],"comparison":[128,164],"other":[131,167,197],"ECMs.The":[132],"results":[133,170],"immunostainig":[135],"no":[139],"significant":[140],"differences":[141],"betweenthe":[142],"experimental":[143],"groups":[144],"finally":[146],"assay":[149],"revealed":[150],"thatdifferentiated":[151],"1:30":[154,183],"matrigel":[155,184],"secreted":[157],"more":[158,186],"responseto":[161],"ECMs.Conclusion:":[168],"Our":[169],"suggest":[171],"that":[172],"type":[173],"ECM":[175],"may":[176],"influence":[177],"insulin-secreting":[180],"effective.":[187],"However,":[188],"thesuccess":[189],"rate":[190],"needs":[193],"further":[194],"investigations":[195],"appropriateECMs.":[198]},"counts_by_year":[{"year":2021,"cited_by_count":1}],"updated_date":"2026-05-06T06:10:43.113611","created_date":"2023-11-03T00:00:00"}