{"id":"https://openalex.org/W4388204792","doi":"https://doi.org/10.1111/j.1470-8744.1995.tb00327.x","title":"The macroheterogeneity of recombinant human interferon\u2010gamma produced by Chinese\u2010hamster ovary cells is affected by the protein and lipid content of the culture medium","display_name":"The macroheterogeneity of recombinant human interferon\u2010gamma produced by Chinese\u2010hamster ovary cells is affected by the protein and lipid content of the culture medium","publication_year":1995,"publication_date":"1995-02-01","ids":{"openalex":"https://openalex.org/W4388204792","doi":"https://doi.org/10.1111/j.1470-8744.1995.tb00327.x","mag":"2054401823","pmid":"https://pubmed.ncbi.nlm.nih.gov/7710705"},"language":"en","primary_location":{"id":"doi:10.1111/j.1470-8744.1995.tb00327.x","doi":"https://doi.org/10.1111/j.1470-8744.1995.tb00327.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1470-8744.1995.tb00327.x","pdf_url":null,"source":{"id":"https://openalex.org/S34872747","display_name":"Biotechnology and Applied Biochemistry","issn_l":"0885-4513","issn":["0885-4513","1470-8744"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Applied Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Castro PM","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I20581793","display_name":"University of Kent","ror":"https://ror.org/00xkeyj56","country_code":"GB","type":"education","lineage":["https://openalex.org/I20581793"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Castro PM","raw_affiliation_strings":["Research School of Biosciences, Biological Laboratory, University of Kent, Canterbury, U.K"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Research School of Biosciences, Biological Laboratory, University of Kent, Canterbury, U.K","institution_ids":["https://openalex.org/I20581793"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110044043","display_name":"Andrew P. Ison","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I20581793","display_name":"University of Kent","ror":"https://ror.org/00xkeyj56","country_code":"GB","type":"education","lineage":["https://openalex.org/I20581793"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Ison AP","raw_affiliation_strings":["Research School of Biosciences, Biological Laboratory, University of Kent, Canterbury, U.K"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Research School of Biosciences, Biological Laboratory, University of Kent, Canterbury, U.K","institution_ids":["https://openalex.org/I20581793"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Hayter PM","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I20581793","display_name":"University of Kent","ror":"https://ror.org/00xkeyj56","country_code":"GB","type":"education","lineage":["https://openalex.org/I20581793"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Hayter PM","raw_affiliation_strings":["Research School of Biosciences, Biological Laboratory, University of Kent, Canterbury, U.K"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Research School of Biosciences, Biological Laboratory, University of Kent, Canterbury, U.K","institution_ids":["https://openalex.org/I20581793"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5006146778","display_name":"Bull At","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I20581793","display_name":"University of Kent","ror":"https://ror.org/00xkeyj56","country_code":"GB","type":"education","lineage":["https://openalex.org/I20581793"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Bull AT","raw_affiliation_strings":["Research School of Biosciences, Biological Laboratory, University of Kent, Canterbury, U.K"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Research School of Biosciences, Biological Laboratory, University of Kent, Canterbury, U.K","institution_ids":["https://openalex.org/I20581793"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I20581793"],"apc_list":{"value":3470,"currency":"USD","value_usd":3470},"apc_paid":null,"fwci":0.6236,"has_fulltext":false,"cited_by_count":46,"citation_normalized_percentile":{"value":0.61017535,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"21","issue":"1","first_page":"87","last_page":"100"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10602","display_name":"Glycosylation and Glycoproteins Research","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/interferon-gamma","display_name":"interferon-gamma","score":0.9110000133514404},{"id":"https://openalex.org/keywords/glycosylation","display_name":"glycosylation","score":0.7639999985694885},{"id":"https://openalex.org/keywords/glycoforms","display_name":"glycoforms","score":0.7900000214576721},{"id":"https://openalex.org/keywords/cho-cells","display_name":"CHO cells","score":0.6710000038146973},{"id":"https://openalex.org/keywords/culture-medium-composition","display_name":"culture medium composition","score":0.8140000104904175},{"id":"https://openalex.org/keywords/serum-free-medium","display_name":"serum-free medium","score":0.5389999747276306},{"id":"https://openalex.org/keywords/bsa","display_name":"BSA","score":0.628000020980835},{"id":"https://openalex.org/keywords/lipid-composition","display_name":"lipid composition","score":0.7289999723434448},{"id":"https://openalex.org/keywords/surfactants","display_name":"surfactants","score":0.9869999885559082},{"id":"https://openalex.org/keywords/glucose","display_name":"glucose","score":0.6039999723434448},{"id":"https://openalex.org/keywords/glutamine","display_name":"glutamine","score":0.9890000224113464},{"id":"https://openalex.org/keywords/batch-feeding","display_name":"batch feeding","score":0.718999981880188},{"id":"https://openalex.org/keywords/process-optimization","display_name":"process optimization","score":0.47200000286102295}],"concepts":[{"id":"https://openalex.org/C175656101","wikidata":"https://www.wikidata.org/wiki/Q56646","display_name":"Chinese hamster ovary cell","level":3,"score":0.9376373887062073},{"id":"https://openalex.org/C2777313579","wikidata":"https://www.wikidata.org/wiki/Q898365","display_name":"Glycosylation","level":2,"score":0.8714214563369751},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.7245157957077026},{"id":"https://openalex.org/C2779349466","wikidata":"https://www.wikidata.org/wiki/Q181619","display_name":"Glutamine","level":3,"score":0.6449650526046753},{"id":"https://openalex.org/C108625454","wikidata":"https://www.wikidata.org/wiki/Q187126","display_name":"Glycoprotein","level":2,"score":0.6135118007659912},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5859320163726807},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.567603588104248},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5609285235404968},{"id":"https://openalex.org/C2780841837","wikidata":"https://www.wikidata.org/wiki/Q24741149","display_name":"Interferon gamma","level":3,"score":0.4997265338897705},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.3735642433166504},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.2763776183128357},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.0},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002461","descriptor_name":"Cell Line, Transformed","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003412","descriptor_name":"Cricetulus","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005947","descriptor_name":"Glucose","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D005973","descriptor_name":"Glutamine","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D006031","descriptor_name":"Glycosylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006224","descriptor_name":"Cricetinae","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007371","descriptor_name":"Interferon-gamma","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D007371","descriptor_name":"Interferon-gamma","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D008055","descriptor_name":"Lipids","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011401","descriptor_name":"Promoter Regions, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012710","descriptor_name":"Serum Albumin, Bovine","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D013501","descriptor_name":"Surface-Active Agents","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D016466","descriptor_name":"CHO Cells","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D016466","descriptor_name":"CHO Cells","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D016466","descriptor_name":"CHO Cells","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D016895","descriptor_name":"Culture Media, Serum-Free","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1111/j.1470-8744.1995.tb00327.x","doi":"https://doi.org/10.1111/j.1470-8744.1995.tb00327.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1470-8744.1995.tb00327.x","pdf_url":null,"source":{"id":"https://openalex.org/S34872747","display_name":"Biotechnology and Applied Biochemistry","issn_l":"0885-4513","issn":["0885-4513","1470-8744"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Applied Biochemistry","raw_type":"journal-article"},{"id":"pmid:7710705","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7710705","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and applied biochemistry","raw_type":"Journal Article"},{"id":"pmh:oai:kar.kent.ac.uk:19748","doi":null,"is_oa":false,"landing_page_url":"https://kar.kent.ac.uk/19748/","pdf_url":null,"source":{"id":"https://openalex.org/S4377196264","display_name":"Kent Academic Repository (University of Kent)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I20581793","host_organization_name":"University of Kent","host_organization_lineage":["https://openalex.org/I20581793"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Article"},{"id":"mag:2054401823","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7710705","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.4043000042438507,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1751195897","https://openalex.org/W2155927328","https://openalex.org/W7013993708","https://openalex.org/W44641968","https://openalex.org/W2029314177","https://openalex.org/W2145283521","https://openalex.org/W158779233","https://openalex.org/W128009662","https://openalex.org/W6982439150","https://openalex.org/W2139132159"],"abstract_inverted_index":{"The":[0,22,170],"culture":[1,136,181],"environment":[2],"exerts":[3],"a":[4,30,47,74],"major":[5,159],"effect":[6],"on":[7,183,189],"the":[8,50,87,93,101,106,112,121,126,131,135,140,145,154,158,167,174,178],"glycosylation":[9,94,116],"pattern":[10,95],"of":[11,29,32,52,81,96,114,123,128,134,142,147,157,164,166,176,180],"recombinant":[12,23],"human":[13],"interferon-gamma":[14],"(IFN-gamma)":[15],"produced":[16],"by":[17,100,119],"Chinese-hamster":[18],"ovary":[19],"(CHO)":[20],"cells.":[21],"IFN-gamma":[24,97,115],"is":[25,46],"heterogeneous":[26],"and":[27,37,41,57,138],"consists":[28],"mixture":[31],"fully":[33,53],"(2N),":[34],"partially":[35],"(1N)":[36],"non-glycosylated":[38],"(0N)":[39],"glycoforms,":[40,160],"throughout":[42],"batch":[43],"cultures":[44,150],"there":[45],"decline":[48,88],"in":[49,64,76,89,105,149,153],"proportion":[51],"glycosylated":[54],"IFN-gamma.":[55],"Glucose":[56],"glutamine,":[58],"nutrients":[59,83],"that":[60,161],"are":[61],"depleted":[62],"early":[63],"such":[65,73],"cultures,":[66],"were":[67],"prima":[68],"facie":[69],"candidates":[70],"for":[71],"causing":[72],"shift":[75],"glycoform":[77],"profile.":[78],"Batch":[79],"feeding":[80],"these":[82],"did":[84],"not":[85],"prevent":[86],"2N":[90],"glycoform,":[91],"but":[92],"was":[98,117,162],"affected":[99,118],"initial":[102],"glutamine":[103],"concentration":[104,122],"culture.":[107],"Under":[108],"different":[109],"serum-free":[110],"environments":[111],"extent":[113],"(1)":[120],"BSA,":[124,129],"(2)":[125],"quality":[127,185],"(3)":[130],"lipid":[132],"composition":[133],"medium":[137],"(4)":[139],"presence":[141],"surfactants.":[143],"Moreover,":[144],"inclusion":[146],"serum":[148],"caused":[151],"changes":[152],"molecular":[155],"masses":[156],"indicative":[163],"cleavage":[165],"core":[168],"polypeptide.":[169],"results":[171],"reported":[172],"emphasize":[173],"necessity":[175],"considering":[177],"effects":[179],"media":[182],"product":[184,190],"as":[186,188],"well":[187],"quantity":[191],"during":[192],"process":[193],"optimization.":[194]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2023-11-02T00:00:00"}