{"id":"https://openalex.org/W4388099472","doi":"https://doi.org/10.1101/2023.10.26.564126","title":"Rhodococcus equi Escapes Macrophage Autophagy: VapA Inhibits ATP6V0d1-Mediated Lysosomal Degradation","display_name":"Rhodococcus equi Escapes Macrophage Autophagy: VapA Inhibits ATP6V0d1-Mediated Lysosomal Degradation","publication_year":2023,"publication_date":"2023-10-30","ids":{"openalex":"https://openalex.org/W4388099472","doi":"https://doi.org/10.1101/2023.10.26.564126"},"language":"en","primary_location":{"id":"doi:10.1101/2023.10.26.564126","doi":"https://doi.org/10.1101/2023.10.26.564126","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2023.10.26.564126","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2023/10/30/2023.10.26.564126.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2023/10/30/2023.10.26.564126.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100781243","display_name":"Haixia Luo","orcid":"https://orcid.org/0000-0002-5038-1994","observed_orcids":["https://orcid.org/0000-0002-5038-1994","https://orcid.org/0009-0005-8234-6092"]},"institutions":[{"id":"https://openalex.org/I21642278","display_name":"Ningxia University","ror":"https://ror.org/04j7b2v61","country_code":"CN","type":"education","lineage":["https://openalex.org/I21642278"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Haixia Luo","raw_affiliation_strings":["Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China, Ningxia University Ningxia Hui Autonomous Region, Yinchuan, 750021, China","Life Science School, Ningxia University, Ningxia Hui Autonomous Region Yinchuan, 750021, China"],"raw_orcid":"https://orcid.org/0000-0002-5038-1994","affiliations":[{"raw_affiliation_string":"Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China, Ningxia University Ningxia Hui Autonomous Region, Yinchuan, 750021, China","institution_ids":["https://openalex.org/I21642278"]},{"raw_affiliation_string":"Life Science School, Ningxia University, Ningxia Hui Autonomous Region Yinchuan, 750021, China","institution_ids":["https://openalex.org/I21642278"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5008338161","display_name":"Tingting Feng","orcid":"https://orcid.org/0000-0002-6282-1555","observed_orcids":["https://orcid.org/0000-0002-6282-1555"]},"institutions":[{"id":"https://openalex.org/I21642278","display_name":"Ningxia University","ror":"https://ror.org/04j7b2v61","country_code":"CN","type":"education","lineage":["https://openalex.org/I21642278"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Tingting Feng","raw_affiliation_strings":["Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China, Ningxia University Ningxia Hui Autonomous Region, Yinchuan, 750021, China","Life Science School, Ningxia University, Ningxia Hui Autonomous Region Yinchuan, 750021, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China, Ningxia University Ningxia Hui Autonomous Region, Yinchuan, 750021, China","institution_ids":["https://openalex.org/I21642278"]},{"raw_affiliation_string":"Life Science School, Ningxia University, Ningxia Hui Autonomous Region Yinchuan, 750021, China","institution_ids":["https://openalex.org/I21642278"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5125101945","display_name":"Zhaokun Xu","orcid":"https://orcid.org/0000-0002-0696-9029","observed_orcids":["https://orcid.org/0000-0002-0696-9029"]},"institutions":[{"id":"https://openalex.org/I21642278","display_name":"Ningxia University","ror":"https://ror.org/04j7b2v61","country_code":"CN","type":"education","lineage":["https://openalex.org/I21642278"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Zhaokun Xu","raw_affiliation_strings":["Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China, Ningxia University Ningxia Hui Autonomous Region, Yinchuan, 750021, China","Life Science School, Ningxia University, Ningxia Hui Autonomous Region Yinchuan, 750021, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China, Ningxia University Ningxia Hui Autonomous Region, Yinchuan, 750021, China","institution_ids":["https://openalex.org/I21642278"]},{"raw_affiliation_string":"Life Science School, Ningxia University, Ningxia Hui Autonomous Region Yinchuan, 750021, China","institution_ids":["https://openalex.org/I21642278"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5100781243"],"corresponding_institution_ids":["https://openalex.org/I21642278"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":1,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12914","display_name":"Infectious Disease Case Reports and Treatments","score":0.9452999830245972,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T12914","display_name":"Infectious Disease Case Reports and Treatments","score":0.9452999830245972,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10587","display_name":"Autophagy in Disease and Therapy","score":0.04340000078082085,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10038","display_name":"Tuberculosis Research and Epidemiology","score":0.0008999999845400453,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/rhodococcus-equi","display_name":"Rhodococcus equi","score":1.0},{"id":"https://openalex.org/keywords/vapa","display_name":"VAPA","score":0.9909999966621399},{"id":"https://openalex.org/keywords/macrophage-autophagy","display_name":"macrophage autophagy","score":0.921999990940094},{"id":"https://openalex.org/keywords/vacuolar-atpase","display_name":"vacuolar ATPase","score":0.8960000276565552},{"id":"https://openalex.org/keywords/lysosomal-acidification","display_name":"lysosomal acidification","score":0.847000002861023},{"id":"https://openalex.org/keywords/phagosome","display_name":"phagosome","score":0.7200000286102295},{"id":"https://openalex.org/keywords/intracellular-bacterial-survival","display_name":"intracellular bacterial survival","score":0.6660000085830688},{"id":"https://openalex.org/keywords/lc3-ii","display_name":"LC3-II","score":0.7429999709129333},{"id":"https://openalex.org/keywords/p62","display_name":"p62","score":0.8629999756813049},{"id":"https://openalex.org/keywords/virulence-plasmid","display_name":"virulence plasmid","score":0.796999990940094},{"id":"https://openalex.org/keywords/foal-pneumonia","display_name":"foal pneumonia","score":0.609000027179718},{"id":"https://openalex.org/keywords/actinomycetes","display_name":"actinomycetes","score":0.7360000014305115}],"concepts":[{"id":"https://openalex.org/C2777581609","wikidata":"https://www.wikidata.org/wiki/Q293148","display_name":"Rhodococcus equi","level":4,"score":0.9529886245727539},{"id":"https://openalex.org/C2779244956","wikidata":"https://www.wikidata.org/wiki/Q184204","display_name":"Macrophage","level":3,"score":0.7778054475784302},{"id":"https://openalex.org/C161478664","wikidata":"https://www.wikidata.org/wiki/Q901955","display_name":"Phagosome","level":3,"score":0.7751548290252686},{"id":"https://openalex.org/C79879829","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Intracellular","level":2,"score":0.6542523503303528},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6480490565299988},{"id":"https://openalex.org/C60987743","wikidata":"https://www.wikidata.org/wiki/Q1460232","display_name":"Virulence","level":3,"score":0.6220979690551758},{"id":"https://openalex.org/C203522944","wikidata":"https://www.wikidata.org/wiki/Q288322","display_name":"Autophagy","level":3,"score":0.5908603668212891},{"id":"https://openalex.org/C153726182","wikidata":"https://www.wikidata.org/wiki/Q3720909","display_name":"Intracellular parasite","level":3,"score":0.558134138584137},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.5482691526412964},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.37026411294937134},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.24130603671073914},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1762840449810028},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.13561594486236572},{"id":"https://openalex.org/C190283241","wikidata":"https://www.wikidata.org/wiki/Q14599311","display_name":"Apoptosis","level":2,"score":0.09566184878349304},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.09328532218933105}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1101/2023.10.26.564126","doi":"https://doi.org/10.1101/2023.10.26.564126","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2023.10.26.564126","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2023/10/30/2023.10.26.564126.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.1101/2023.10.26.564126","doi":"https://doi.org/10.1101/2023.10.26.564126","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2023.10.26.564126","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2023/10/30/2023.10.26.564126.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.5683000087738037,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G21793028","display_name":"VapA \u5728\u9a6c\u7ea2\u7403\u83cc\u9003\u9038\u5de8\u566c\u7ec6\u80de\u81ea\u566c\u4e2d\u7684\u4f5c\u7528\u53ca\u673a\u5236\u7814\u7a76","funder_award_id":"31760035","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G272232476","display_name":"\u80de\u5916\u56ca\u6ce1\u5728\u9a6c\u7ea2\u7403\u83cc\u9003\u9038\u5de8\u566c\u7ec6\u80de\u6e05\u9664\u4e2d\u7684\u4f5c\u7528\u673a\u5236\u7814\u7a76","funder_award_id":"31960694","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"}],"funders":[{"id":"https://openalex.org/F4320321001","display_name":"National Natural Science Foundation of China","ror":"https://ror.org/01h0zpd94"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4388099472.pdf","grobid_xml":"https://content.openalex.org/works/W4388099472.grobid-xml"},"referenced_works_count":17,"referenced_works":["https://openalex.org/W1977109589","https://openalex.org/W2001312439","https://openalex.org/W2031843184","https://openalex.org/W2051062186","https://openalex.org/W2069444935","https://openalex.org/W2121526389","https://openalex.org/W2127021716","https://openalex.org/W2155847201","https://openalex.org/W2159761795","https://openalex.org/W2607224621","https://openalex.org/W2766243612","https://openalex.org/W2773892411","https://openalex.org/W2892957370","https://openalex.org/W3134372233","https://openalex.org/W3157350912","https://openalex.org/W4206349297","https://openalex.org/W4220680847"],"related_works":["https://openalex.org/W4389161422","https://openalex.org/W2111698215","https://openalex.org/W2892957370","https://openalex.org/W4315620485","https://openalex.org/W2773892411","https://openalex.org/W2790011191","https://openalex.org/W4320710502","https://openalex.org/W2539441240","https://openalex.org/W2895715909","https://openalex.org/W2121526389"],"abstract_inverted_index":{"Abstract":[0],"Rhodococcus":[1],"equi":[2,181,191,204,225,248],"is":[3,27],"a":[4,10,55,213],"gram-positive":[5],"actinomycetales":[6],"bacterial,":[7],"which":[8,244],"cause":[9],"pneumonia":[11],"in":[12,15,32,40,82,120,148,172,223],"foals":[13],"and":[14,48,95,115,117,128],"immunocompromised":[16],"humans.":[17],"Virulence":[18],"associated":[19],"protein":[20,113],"A":[21],"(VapA)":[22],"encoded":[23],"from":[24,46],"virulence":[25],"plasmid":[26],"essential":[28],"for":[29],"intracellular":[30,207],"proliferation":[31],"macrophage.":[33,123],"It":[34],"has":[35],"reported":[36,232],"that":[37,100,233],"VapA":[38,81,101,121,146,222,234],"participates":[39],"exclusion":[41],"of":[42,80,110,168,197,199,215,221,242],"proton-pumping":[43],"vacuolar-ATPase":[44],"complex":[45],"phagosome":[47,57],"causes":[49],"membrane":[50],"permeabilization,":[51],"thus":[52],"contributing":[53,245],"to":[54,76,131,141],"pH-neutral":[56],"lumen.":[58],"However,":[59],"there":[60],"have":[61],"been":[62],"no":[63],"reports":[64],"about":[65],"VapA-induced":[66],"autophagy":[67,84,105,111,118],"or":[68],"its":[69,143],"mechanism.":[70],"In":[71,164],"this":[72],"study,":[73],"we":[74,125,231],"sought":[75],"determine":[77],"the":[78,103,108,153,166,219,240,246],"role":[79],"macrophage":[83,104,250],"using":[85,126],"western":[86],"blotting,":[87],"immunofluorescence,":[88],"pull-down,":[89],"Co-IP,":[90],"LS/MS,":[91],"pH":[92],"detection":[93],"LysoTracker":[94],"CFU":[96],"analysis.":[97],"We":[98],"show":[99],"inhibiting":[102,239],"by":[106,185,238],"detecting":[107],"expression":[109,167,241],"related":[112],"LC3II":[114],"P62":[116],"flux":[119],"treated":[122],"Furthermore,":[124,145],"pull-down":[127],"MS":[129],"analysis":[130],"selected":[132],"ATP6V0D1":[133,149,160,169,200,216],"could":[134],"interact":[135],"with":[136,159,176,187],"VapA,":[137],"Co-IP":[138],"was":[139],"used":[140],"confirm":[142],"interaction.":[144],"treatment":[147],"overexpressed":[150,161],"cells":[151],"weaken":[152],"lyso":[154],"Tracker":[155],"red":[156],"stain":[157],"comparing":[158,186],"J774A.1":[162,173],"cells.":[163],"addition,":[165],"significantly":[170],"increased":[171],"post":[174],"infected":[175],"vapA":[177],"deletion":[178],"strain,":[179],"R.":[180,190,203,224,247],"103":[182,192,205],"+":[183,193,206],"/\u0394VapA":[184],"wild":[188],"type":[189],"post-infection.":[194],"And":[195],"raise":[196,198],"actually":[201],"reduce":[202],"multiplication.":[208],"These":[209],"results":[210],"further":[211],"suggesting":[212],"decrease":[214],"were":[217],"caused":[218],"exit":[220],".":[226],"Taken":[227],"those":[228],"date":[229],"together,":[230],"mediated":[235],"lysosome":[236],"acidification":[237],"ATP6V0D1,":[243],"escape":[249],"autophagy.":[251]},"counts_by_year":[{"year":2024,"cited_by_count":1}],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2023-11-01T00:00:00"}
