{"id":"https://openalex.org/W4387968481","doi":"https://doi.org/10.3791/65733","title":"Optogenetic Signaling Activation in Zebrafish Embryos","display_name":"Optogenetic Signaling Activation in Zebrafish Embryos","publication_year":2023,"publication_date":"2023-10-27","ids":{"openalex":"https://openalex.org/W4387968481","doi":"https://doi.org/10.3791/65733","pmid":"https://pubmed.ncbi.nlm.nih.gov/37955383","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/12284804"},"language":"en","primary_location":{"id":"doi:10.3791/65733","doi":"https://doi.org/10.3791/65733","is_oa":true,"landing_page_url":"https://doi.org/10.3791/65733","pdf_url":"https://www.jove.com/pdf/65733/author-spotlight-manipulating-signaling-zebrafish-embryos-to-decode","source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://www.jove.com/pdf/65733/author-spotlight-manipulating-signaling-zebrafish-embryos-to-decode","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Allison J. Saul","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210144228","display_name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","ror":"https://ror.org/04byxyr05","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210144228"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Allison J. Saul","raw_affiliation_strings":["Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210144228"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Catherine E. Rogers","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210144228","display_name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","ror":"https://ror.org/04byxyr05","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210144228"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Catherine E. Rogers","raw_affiliation_strings":["Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210144228"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015007533","display_name":"Marcial Garmendia\u2010Cedillos","orcid":"https://orcid.org/0000-0002-1013-1835","observed_orcids":["https://orcid.org/0000-0002-1013-1835"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210088243","display_name":"National Institute of Biomedical Imaging and Bioengineering","ror":"https://ror.org/00372qc85","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210088243"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Marcial Garmendia-Cedillos","raw_affiliation_strings":["Instrumentation Development and Engineering Application Solutions, National Institute of Biomedical Imaging and Bioengineering, NIH"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Instrumentation Development and Engineering Application Solutions, National Institute of Biomedical Imaging and Bioengineering, NIH","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210088243"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5020138295","display_name":"Thomas J. Pohida","orcid":"https://orcid.org/0000-0002-6093-2251","observed_orcids":["https://orcid.org/0000-0002-6093-2251"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210088243","display_name":"National Institute of Biomedical Imaging and Bioengineering","ror":"https://ror.org/00372qc85","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210088243"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Thomas Pohida","raw_affiliation_strings":["Instrumentation Development and Engineering Application Solutions, National Institute of Biomedical Imaging and Bioengineering, NIH"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Instrumentation Development and Engineering Application Solutions, National Institute of Biomedical Imaging and Bioengineering, NIH","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210088243"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5088018907","display_name":"Katherine W. Rogers","orcid":"https://orcid.org/0000-0001-5700-2662","observed_orcids":["https://orcid.org/0000-0001-5700-2662"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210144228","display_name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","ror":"https://ror.org/04byxyr05","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210144228"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Katherine W. Rogers","raw_affiliation_strings":["Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH; katherine.rogers@nih.gov","Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH; katherine.rogers@nih.gov","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210144228"]},{"raw_affiliation_string":"Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210144228"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.5585,"has_fulltext":true,"cited_by_count":4,"citation_normalized_percentile":{"value":0.57722344,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":98},"biblio":{"volume":null,"issue":"200","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11685","display_name":"Zebrafish Biomedical Research Applications","score":0.6937999725341797,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11685","display_name":"Zebrafish Biomedical Research Applications","score":0.6937999725341797,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10268","display_name":"Developmental Biology and Gene Regulation","score":0.10029999911785126,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12236","display_name":"Photoreceptor and optogenetics research","score":0.0934000015258789,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/optogenetics","display_name":"optogenetics","score":0.9739999771118164},{"id":"https://openalex.org/keywords/zebrafish-embryos","display_name":"zebrafish embryos","score":0.7889999747276306},{"id":"https://openalex.org/keywords/lov-domain","display_name":"LOV domain","score":0.746999979019165},{"id":"https://openalex.org/keywords/bmp-signaling","display_name":"BMP signaling","score":0.8700000047683716},{"id":"https://openalex.org/keywords/nodal-signaling","display_name":"Nodal signaling","score":0.9980000257492065},{"id":"https://openalex.org/keywords/early-embryonic-development","display_name":"early embryonic development","score":0.718999981880188}],"concepts":[{"id":"https://openalex.org/C50738837","wikidata":"https://www.wikidata.org/wiki/Q781492","display_name":"Optogenetics","level":2,"score":0.8361448049545288},{"id":"https://openalex.org/C2776878037","wikidata":"https://www.wikidata.org/wiki/Q169444","display_name":"Zebrafish","level":3,"score":0.8128598928451538},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.6091765761375427},{"id":"https://openalex.org/C205583638","wikidata":"https://www.wikidata.org/wiki/Q7046607","display_name":"Nodal signaling","level":5,"score":0.5954300761222839},{"id":"https://openalex.org/C62478195","wikidata":"https://www.wikidata.org/wiki/Q828130","display_name":"Signal transduction","level":2,"score":0.5932247638702393},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5488725900650024},{"id":"https://openalex.org/C31507581","wikidata":"https://www.wikidata.org/wiki/Q425383","display_name":"Bone morphogenetic protein","level":3,"score":0.46284979581832886},{"id":"https://openalex.org/C51785407","wikidata":"https://www.wikidata.org/wiki/Q2627568","display_name":"Effector","level":2,"score":0.4271789789199829},{"id":"https://openalex.org/C169760540","wikidata":"https://www.wikidata.org/wiki/Q207011","display_name":"Neuroscience","level":1,"score":0.2457955777645111},{"id":"https://openalex.org/C196843134","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryo","level":2,"score":0.21574807167053223},{"id":"https://openalex.org/C87073359","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryogenesis","level":3,"score":0.1552748680114746},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.09002184867858887},{"id":"https://openalex.org/C129536746","wikidata":"https://www.wikidata.org/wiki/Q1141026","display_name":"Gastrulation","level":4,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000072417","descriptor_name":"Protein Domains","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008027","descriptor_name":"Light","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015027","descriptor_name":"Zebrafish","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D015398","descriptor_name":"Signal Transduction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D029961","descriptor_name":"Zebrafish Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D029961","descriptor_name":"Zebrafish Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D062308","descriptor_name":"Optogenetics","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.3791/65733","doi":"https://doi.org/10.3791/65733","is_oa":true,"landing_page_url":"https://doi.org/10.3791/65733","pdf_url":"https://www.jove.com/pdf/65733/author-spotlight-manipulating-signaling-zebrafish-embryos-to-decode","source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},{"id":"pmid:37955383","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/37955383","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of visualized experiments : JoVE","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:12284804","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/12284804","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC12284804/pdf/nihms-2090438.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"J Vis Exp","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.3791/65733","doi":"https://doi.org/10.3791/65733","is_oa":true,"landing_page_url":"https://doi.org/10.3791/65733","pdf_url":"https://www.jove.com/pdf/65733/author-spotlight-manipulating-signaling-zebrafish-embryos-to-decode","source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2351566894","display_name":"Decoding developmental signaling during zebrafish embryogenesis","funder_award_id":"1ziahd009002-03","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G9000243239","display_name":null,"funder_award_id":"ZIA HD009002","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4387968481.pdf","grobid_xml":"https://content.openalex.org/works/W4387968481.grobid-xml"},"referenced_works_count":65,"referenced_works":["https://openalex.org/W1489916230","https://openalex.org/W1866582684","https://openalex.org/W1963917095","https://openalex.org/W1974487330","https://openalex.org/W1988165722","https://openalex.org/W1988289255","https://openalex.org/W1992182416","https://openalex.org/W1996551832","https://openalex.org/W1998766660","https://openalex.org/W2000157853","https://openalex.org/W2008915544","https://openalex.org/W2037143749","https://openalex.org/W2047346477","https://openalex.org/W2051042430","https://openalex.org/W2058391957","https://openalex.org/W2097901077","https://openalex.org/W2109067263","https://openalex.org/W2125349610","https://openalex.org/W2136430787","https://openalex.org/W2136732727","https://openalex.org/W2140443734","https://openalex.org/W2235988210","https://openalex.org/W2322464591","https://openalex.org/W2467474458","https://openalex.org/W2528893335","https://openalex.org/W2564496757","https://openalex.org/W2580093245","https://openalex.org/W2582128419","https://openalex.org/W2602977838","https://openalex.org/W2605647953","https://openalex.org/W2775399421","https://openalex.org/W2788530992","https://openalex.org/W2793103288","https://openalex.org/W2794262744","https://openalex.org/W2809533253","https://openalex.org/W2885557322","https://openalex.org/W2900419795","https://openalex.org/W2908607257","https://openalex.org/W2912421035","https://openalex.org/W2946400198","https://openalex.org/W2950434985","https://openalex.org/W2950545321","https://openalex.org/W2951869480","https://openalex.org/W2953942915","https://openalex.org/W2990887864","https://openalex.org/W2992419145","https://openalex.org/W2995005073","https://openalex.org/W3012986776","https://openalex.org/W3025942091","https://openalex.org/W3042131965","https://openalex.org/W3044605296","https://openalex.org/W3104021522","https://openalex.org/W3128751311","https://openalex.org/W3131102773","https://openalex.org/W3194850334","https://openalex.org/W3196396807","https://openalex.org/W4200563105","https://openalex.org/W4213351467","https://openalex.org/W4214708202","https://openalex.org/W4214877734","https://openalex.org/W4220875955","https://openalex.org/W4226269238","https://openalex.org/W4281643512","https://openalex.org/W4292683996","https://openalex.org/W7096294232"],"related_works":["https://openalex.org/W4409727604","https://openalex.org/W4408885539","https://openalex.org/W7212052560","https://openalex.org/W3210131860","https://openalex.org/W2995005073","https://openalex.org/W3033764814","https://openalex.org/W3093824095","https://openalex.org/W2487146662","https://openalex.org/W2948263226","https://openalex.org/W4319936696"],"abstract_inverted_index":{"Signaling":[0],"pathways":[1],"orchestrate":[2],"fundamental":[3],"biological":[4],"processes,":[5],"including":[6],"development,":[7,164],"regeneration,":[8],"homeostasis,":[9],"and":[10,48,56,98,103,126,166],"disease.":[11],"Methods":[12],"to":[13,19,78,121,130,157],"experimentally":[14],"manipulate":[15],"signaling":[16,22,38,76,100,162],"are":[17],"required":[18],"understand":[20],"how":[21],"is":[23],"interpreted":[24],"in":[25,52,57,105,147,163],"these":[26,135],"wide-ranging":[27],"contexts.":[28],"Molecular":[29],"optogenetic":[30,145],"tools":[31,60,146],"can":[32,138],"provide":[33,153],"reversible,":[34],"tunable":[35],"manipulations":[36],"of":[37,45,161],"pathway":[39],"activity":[40],"with":[41,75],"a":[42,141,154],"high":[43],"degree":[44],"spatiotemporal":[46],"control":[47,82,115,136],"have":[49],"been":[50],"applied":[51],"vitro,":[53],"ex":[54],"vivo,":[55],"vivo.":[58],"These":[59,151],"couple":[61],"light-responsive":[62],"protein":[63,96],"domains,":[64],"such":[65],"as":[66],"the":[67,92,106,159],"blue":[68],"light":[69],"homodimerizing":[70],"light-oxygen-voltage":[71],"sensing":[72],"(LOV)":[73],"domain,":[74],"effectors":[77],"confer":[79],"light-dependent":[80],"experimental":[81,124],"over":[83],"signaling.":[84,133],"This":[85],"protocol":[86],"provides":[87],"practical":[88],"guidelines":[89],"for":[90,143],"using":[91,144],"LOV-based":[93],"bone":[94],"morphogenetic":[95],"(BMP)":[97],"Nodal":[99],"activators":[101],"bOpto-BMP":[102],"bOpto-Nodal":[104],"optically":[107],"accessible":[108],"early":[109,148],"zebrafish":[110,149],"embryo.":[111],"It":[112],"describes":[113],"two":[114],"experiments:":[116],"A":[117],"quick":[118],"phenotype":[119],"assay":[120,129],"determine":[122],"appropriate":[123],"conditions,":[125],"an":[127],"immunofluorescence":[128],"directly":[131],"assess":[132],"Together,":[134],"experiments":[137],"help":[139],"establish":[140],"pipeline":[142],"embryos.":[150],"strategies":[152],"powerful":[155],"platform":[156],"investigate":[158],"roles":[160],"health,":[165],"physiology.":[167]},"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2024,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2023-10-28T00:00:00"}
