{"id":"https://openalex.org/W4387515982","doi":"https://doi.org/10.1101/pdb.prot108194","title":"Generating and Validating Transgenic Mosquitoes with Transposon-Mediated Transgenesis","display_name":"Generating and Validating Transgenic Mosquitoes with Transposon-Mediated Transgenesis","publication_year":2023,"publication_date":"2023-10-10","ids":{"openalex":"https://openalex.org/W4387515982","doi":"https://doi.org/10.1101/pdb.prot108194","pmid":"https://pubmed.ncbi.nlm.nih.gov/37816603","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/11008536"},"language":"en","primary_location":{"id":"doi:10.1101/pdb.prot108194","doi":"https://doi.org/10.1101/pdb.prot108194","is_oa":false,"landing_page_url":"https://doi.org/10.1101/pdb.prot108194","pdf_url":null,"source":{"id":"https://openalex.org/S119953358","display_name":"Cold Spring Harbor Protocols","issn_l":"1559-6095","issn":["1559-6095","1940-3402"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315909","host_organization_name":"Cold Spring Harbor Laboratory Press","host_organization_lineage":["https://openalex.org/P4310315909"],"host_organization_lineage_names":["Cold Spring Harbor Laboratory Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cold Spring Harbor Protocols","raw_type":"journal-article"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC11008536/pdf/nihms-1983187.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5019581372","display_name":"Vanessa Bottino-Rojas","orcid":"https://orcid.org/0000-0001-8019-9942","observed_orcids":["https://orcid.org/0000-0001-8019-9942"]},"institutions":[{"id":"https://openalex.org/I204250578","display_name":"University of California, Irvine","ror":"https://ror.org/04gyf1771","country_code":"US","type":"education","lineage":["https://openalex.org/I204250578"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Vanessa Bottino-Rojas","raw_affiliation_strings":["Department of Microbiology and Molecular Genetics, University of California, Irvine, California 92697-4500, USA","1Department of Microbiology and Molecular Genetics, University of California, Irvine, California 92697-4500, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology and Molecular Genetics, University of California, Irvine, California 92697-4500, USA","institution_ids":["https://openalex.org/I204250578"]},{"raw_affiliation_string":"1Department of Microbiology and Molecular Genetics, University of California, Irvine, California 92697-4500, USA","institution_ids":["https://openalex.org/I204250578"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5039756363","display_name":"Anthony Amade James","orcid":"https://orcid.org/0000-0001-5577-3308","observed_orcids":["https://orcid.org/0000-0001-5577-3308"]},"institutions":[{"id":"https://openalex.org/I204250578","display_name":"University of California, Irvine","ror":"https://ror.org/04gyf1771","country_code":"US","type":"education","lineage":["https://openalex.org/I204250578"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Anthony A. James","raw_affiliation_strings":["Department of Microbiology and Molecular Genetics, University of California, Irvine, California 92697-4500, USA aajames@uci.edu","Department of Molecular Biology and Biochemistry, University of California, Irvine, California 92697-3900, USA","1Department of Microbiology and Molecular Genetics, University of California, Irvine, California 92697-4500, USA","2Department of Molecular Biology and Biochemistry, University of California, Irvine, California 92697-3900, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology and Molecular Genetics, University of California, Irvine, California 92697-4500, USA aajames@uci.edu","institution_ids":["https://openalex.org/I204250578"]},{"raw_affiliation_string":"Department of Molecular Biology and Biochemistry, University of California, Irvine, California 92697-3900, USA","institution_ids":["https://openalex.org/I204250578"]},{"raw_affiliation_string":"1Department of Microbiology and Molecular Genetics, University of California, Irvine, California 92697-4500, USA","institution_ids":["https://openalex.org/I204250578"]},{"raw_affiliation_string":"2Department of Molecular Biology and Biochemistry, University of California, Irvine, California 92697-3900, USA","institution_ids":["https://openalex.org/I204250578"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5039756363"],"corresponding_institution_ids":["https://openalex.org/I204250578"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":2,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"2024","issue":"10","first_page":"pdb.prot108194","last_page":"pdb.prot108194"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10166","display_name":"Mosquito-borne diseases and control","score":0.2957000136375427,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10166","display_name":"Mosquito-borne diseases and control","score":0.2957000136375427,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12929","display_name":"Animal Genetics and Reproduction","score":0.2639000117778778,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.11150000244379044,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/transgenic-mosquitoes","display_name":"transgenic mosquitoes","score":0.9670000076293945},{"id":"https://openalex.org/keywords/transposon-tagging","display_name":"transposon tagging","score":0.8349999785423279},{"id":"https://openalex.org/keywords/enhancer-trap","display_name":"enhancer trap","score":0.8119999766349792},{"id":"https://openalex.org/keywords/insertional-mutagenesis","display_name":"insertional mutagenesis","score":0.9399999976158142}],"concepts":[{"id":"https://openalex.org/C4918238","wikidata":"https://www.wikidata.org/wiki/Q121438","display_name":"Transposable element","level":4,"score":0.8590338230133057},{"id":"https://openalex.org/C73247094","wikidata":"https://www.wikidata.org/wiki/Q2790163","display_name":"Transgenesis","level":5,"score":0.8486819267272949},{"id":"https://openalex.org/C107635520","wikidata":"https://www.wikidata.org/wiki/Q1967554","display_name":"Insertional mutagenesis","level":4,"score":0.7356352806091309},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7200761437416077},{"id":"https://openalex.org/C28693550","wikidata":"https://www.wikidata.org/wiki/Q7835335","display_name":"Transposon mutagenesis","level":5,"score":0.641014039516449},{"id":"https://openalex.org/C133288520","wikidata":"https://www.wikidata.org/wiki/Q7539848","display_name":"Sleeping Beauty transposon system","level":5,"score":0.5890530347824097},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.5251526236534119},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4923168420791626},{"id":"https://openalex.org/C102230213","wikidata":"https://www.wikidata.org/wiki/Q430117","display_name":"Transgene","level":3,"score":0.4773828387260437},{"id":"https://openalex.org/C111936080","wikidata":"https://www.wikidata.org/wiki/Q913367","display_name":"Enhancer","level":4,"score":0.4755936563014984},{"id":"https://openalex.org/C16318435","wikidata":"https://www.wikidata.org/wiki/Q149299","display_name":"Mutagenesis","level":4,"score":0.46395793557167053},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.33235234022140503},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.167341947555542},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.14116054773330688},{"id":"https://openalex.org/C519853106","wikidata":"https://www.wikidata.org/wiki/Q3640158","display_name":"Reproductive biology","level":4,"score":0.0},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.0},{"id":"https://openalex.org/C87073359","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryogenesis","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D000072138","descriptor_name":"Mosquito Vectors","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004251","descriptor_name":"DNA Transposable Elements","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D009033","descriptor_name":"Culicidae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D016254","descriptor_name":"Mutagenesis, Insertional","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D018014","descriptor_name":"Gene Transfer Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D030801","descriptor_name":"Animals, Genetically Modified","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1101/pdb.prot108194","doi":"https://doi.org/10.1101/pdb.prot108194","is_oa":false,"landing_page_url":"https://doi.org/10.1101/pdb.prot108194","pdf_url":null,"source":{"id":"https://openalex.org/S119953358","display_name":"Cold Spring Harbor Protocols","issn_l":"1559-6095","issn":["1559-6095","1940-3402"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315909","host_organization_name":"Cold Spring Harbor Laboratory Press","host_organization_lineage":["https://openalex.org/P4310315909"],"host_organization_lineage_names":["Cold Spring Harbor Laboratory Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cold Spring Harbor Protocols","raw_type":"journal-article"},{"id":"pmid:37816603","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/37816603","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cold Spring Harbor protocols","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:11008536","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/11008536","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC11008536/pdf/nihms-1983187.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Cold Spring Harb Protoc","raw_type":"Text"},{"id":"pmh:oai:escholarship.org:ark:/13030/qt9q2113dv","doi":null,"is_oa":true,"landing_page_url":"https://escholarship.org/uc/item/9q2113dv","pdf_url":"https://escholarship.org/content/qt9q2113dv/qt9q2113dv.pdf","source":{"id":"https://openalex.org/S4306400115","display_name":"eScholarship (California Digital Library)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2801248553","host_organization_name":"California Digital Library","host_organization_lineage":["https://openalex.org/I2801248553"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Cold Spring Harbor Protocols, vol 2024, iss 10","raw_type":"article"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:11008536","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/11008536","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC11008536/pdf/nihms-1983187.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Cold Spring Harb Protoc","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1645478234","display_name":"Expression of Exogenous Genes in Vector Mosquitoes","funder_award_id":"R01AI029746","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"},{"id":"https://openalex.org/G9000877618","display_name":"Impact of gene-drive systems for population modification on malaria vector mosquitoes","funder_award_id":"R01AI170692","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"}],"funders":[{"id":"https://openalex.org/F4320332688","display_name":"University of California, Irvine","ror":"https://ror.org/04gyf1771"},{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4387515982.pdf","grobid_xml":"https://content.openalex.org/works/W4387515982.grobid-xml"},"referenced_works_count":23,"referenced_works":["https://openalex.org/W1534179616","https://openalex.org/W1970646555","https://openalex.org/W1975724661","https://openalex.org/W1977160885","https://openalex.org/W1986552726","https://openalex.org/W1993097572","https://openalex.org/W1998475833","https://openalex.org/W2053350421","https://openalex.org/W2062207811","https://openalex.org/W2077986699","https://openalex.org/W2108452174","https://openalex.org/W2112400573","https://openalex.org/W2174714366","https://openalex.org/W2188921448","https://openalex.org/W2193418846","https://openalex.org/W2272803216","https://openalex.org/W2472255557","https://openalex.org/W3128020305","https://openalex.org/W3182916335","https://openalex.org/W4234128816","https://openalex.org/W4387326186","https://openalex.org/W4387326197","https://openalex.org/W4387515783"],"related_works":["https://openalex.org/W4248533321","https://openalex.org/W4387515783","https://openalex.org/W4413305786","https://openalex.org/W2112400573","https://openalex.org/W2287860811","https://openalex.org/W1507560730","https://openalex.org/W2519394601","https://openalex.org/W2087890854","https://openalex.org/W2254805474","https://openalex.org/W2032706124"],"abstract_inverted_index":{"Transposon-mediated":[0],"transgenesis":[1],"has":[2],"revolutionized":[3],"both":[4],"basic":[5],"and":[6,20,43,59,77,80,84],"applied":[7],"studies":[8],"of":[9,12,41,45,61,72],"mosquito":[10,47,57,66],"vectors":[11],"disease.":[13],"Currently,":[14],"techniques":[15],"such":[16],"as":[17],"enhancer":[18],"traps":[19],"transposon":[21],"tagging,":[22],"which":[23],"rely":[24],"on":[25],"remobilizable":[26],"insertional":[27],"mutagenesis,":[28],"are":[29,68,82],"only":[30],"possible":[31],"with":[32],"transposon-based":[33,46],"vector":[34],"systems.":[35],"Here,":[36],"we":[37],"provide":[38],"general":[39],"descriptions":[40],"methods":[42,81],"applications":[44],"transgenesis.":[48],"The":[49],"exact":[50],"procedures":[51],"must":[52],"be":[53],"adapted":[54],"to":[55],"each":[56],"species":[58,67],"comparisons":[60],"some":[62],"differences":[63],"among":[64],"different":[65],"outlined.":[69],"A":[70],"number":[71],"excellent":[73],"publications":[74],"showing":[75],"detailed":[76],"specific":[78],"protocols":[79],"featured":[83],"referenced.":[85]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2023,"cited_by_count":1}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2023-10-12T00:00:00"}
