{"id":"https://openalex.org/W4387241978","doi":"https://doi.org/10.1111/1556-4029.15371","title":"Alternative direct\u2010to\u2010amplification cell lysis techniques for forensically relevant non\u2010sperm cells","display_name":"Alternative direct\u2010to\u2010amplification cell lysis techniques for forensically relevant non\u2010sperm cells","publication_year":2023,"publication_date":"2023-10-01","ids":{"openalex":"https://openalex.org/W4387241978","doi":"https://doi.org/10.1111/1556-4029.15371","pmid":"https://pubmed.ncbi.nlm.nih.gov/37779342"},"language":"en","primary_location":{"id":"doi:10.1111/1556-4029.15371","doi":"https://doi.org/10.1111/1556-4029.15371","is_oa":true,"landing_page_url":"http://dx.doi.org/10.1111/1556-4029.15371","pdf_url":null,"source":{"id":"https://openalex.org/S62072220","display_name":"Journal of Forensic Sciences","issn_l":"0022-1198","issn":["0022-1198","1556-4029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Forensic Sciences","raw_type":"journal-article"},"parent_works":[],"lineage":["https://openalex.org/W4387241978"],"child_works_count":0,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"http://dx.doi.org/10.1111/1556-4029.15371","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5102987815","display_name":"Rhea Arya","orcid":"https://orcid.org/0009-0008-2089-8539","observed_orcids":["https://orcid.org/0009-0008-2089-8539"]},"institutions":[{"id":"https://openalex.org/I184840846","display_name":"Virginia Commonwealth University","ror":"https://ror.org/02nkdxk79","country_code":"US","type":"education","lineage":["https://openalex.org/I184840846"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Rhea Arya","raw_affiliation_strings":["Department of Forensic Science, Virginia Commonwealth University, Richmond, Virginia, USA"],"raw_orcid":"https://orcid.org/0009-0008-2089-8539","affiliations":[{"raw_affiliation_string":"Department of Forensic Science, Virginia Commonwealth University, Richmond, Virginia, USA","institution_ids":["https://openalex.org/I184840846"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5000161382","display_name":"Brittany C. Hudson","orcid":"https://orcid.org/0000-0003-1526-0537","observed_orcids":["https://orcid.org/0000-0003-1526-0537"]},"institutions":[{"id":"https://openalex.org/I184840846","display_name":"Virginia Commonwealth University","ror":"https://ror.org/02nkdxk79","country_code":"US","type":"education","lineage":["https://openalex.org/I184840846"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Brittany C. Hudson","raw_affiliation_strings":["Department of Forensic Science, Virginia Commonwealth University, Richmond, Virginia, USA","Integrative Life Sciences, Virginia Commonwealth University, Richmond, Virginia, USA"],"raw_orcid":"https://orcid.org/0000-0003-1526-0537","affiliations":[{"raw_affiliation_string":"Department of Forensic Science, Virginia Commonwealth University, Richmond, Virginia, USA","institution_ids":["https://openalex.org/I184840846"]},{"raw_affiliation_string":"Integrative Life Sciences, Virginia Commonwealth University, Richmond, Virginia, USA","institution_ids":["https://openalex.org/I184840846"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5005992739","display_name":"Tracey Dawson Cruz","orcid":"https://orcid.org/0000-0003-4761-6657","observed_orcids":["https://orcid.org/0000-0003-4761-6657"]},"institutions":[{"id":"https://openalex.org/I184840846","display_name":"Virginia Commonwealth University","ror":"https://ror.org/02nkdxk79","country_code":"US","type":"education","lineage":["https://openalex.org/I184840846"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Tracey Dawson Green","raw_affiliation_strings":["Department of Forensic Science, Virginia Commonwealth University, Richmond, Virginia, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Forensic Science, Virginia Commonwealth University, Richmond, Virginia, USA","institution_ids":["https://openalex.org/I184840846"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5005992739","https://openalex.org/A5102987815"],"corresponding_institution_ids":["https://openalex.org/I184840846"],"apc_list":{"value":4120,"currency":"USD","value_usd":4120},"apc_paid":{"value":4120,"currency":"USD","value_usd":4120},"fwci":0.1187,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":{"value":0.31544751,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":91,"max":95},"biblio":{"volume":"68","issue":"6","first_page":"2116","last_page":"2127"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10751","display_name":"Forensic and Genetic Research","score":0.9951000213623047,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10751","display_name":"Forensic and Genetic Research","score":0.9951000213623047,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.002300000051036477,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10012","display_name":"Genetic diversity and population structure","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/sexual-assault-evidence","display_name":"sexual assault evidence","score":0.7509999871253967},{"id":"https://openalex.org/keywords/vaginal-epithelial-cells","display_name":"vaginal epithelial cells","score":0.8759999871253967},{"id":"https://openalex.org/keywords/alkaline-lysis","display_name":"alkaline lysis","score":0.8379999995231628},{"id":"https://openalex.org/keywords/str-profiling","display_name":"STR profiling","score":0.5690000057220459},{"id":"https://openalex.org/keywords/dna-quantification","display_name":"DNA quantification","score":0.5109999775886536},{"id":"https://openalex.org/keywords/forensic-dna-analysis","display_name":"forensic DNA analysis","score":0.48500001430511475}],"concepts":[{"id":"https://openalex.org/C57409179","wikidata":"https://www.wikidata.org/wiki/Q1218293","display_name":"Lysis","level":2,"score":0.911506175994873},{"id":"https://openalex.org/C2781087480","wikidata":"https://www.wikidata.org/wiki/Q17145","display_name":"Sperm","level":2,"score":0.7655818462371826},{"id":"https://openalex.org/C190431759","wikidata":"https://www.wikidata.org/wiki/Q6710242","display_name":"Lysis buffer","level":3,"score":0.6433703899383545},{"id":"https://openalex.org/C2778093475","wikidata":"https://www.wikidata.org/wiki/Q9715","display_name":"Semen","level":2,"score":0.6318168640136719},{"id":"https://openalex.org/C20808630","wikidata":"https://www.wikidata.org/wiki/Q4727661","display_name":"Alkaline lysis","level":5,"score":0.5043991804122925},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.48464471101760864},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.4750761389732361},{"id":"https://openalex.org/C16685009","wikidata":"https://www.wikidata.org/wiki/Q335057","display_name":"Andrology","level":1,"score":0.44772863388061523},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.36270397901535034},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3488582968711853},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.20809829235076904},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.19648420810699463},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.12603828310966492},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.10640570521354675},{"id":"https://openalex.org/C167508917","wikidata":"https://www.wikidata.org/wiki/Q578537","display_name":"DNA vaccination","level":4,"score":0.0},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007202","descriptor_name":"Indicators and Reagents","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012661","descriptor_name":"Semen","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012972","descriptor_name":"Sodium Hydroxide","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013094","descriptor_name":"Spermatozoa","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016172","descriptor_name":"DNA Fingerprinting","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D018895","descriptor_name":"Microsatellite Repeats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1111/1556-4029.15371","doi":"https://doi.org/10.1111/1556-4029.15371","is_oa":true,"landing_page_url":"http://dx.doi.org/10.1111/1556-4029.15371","pdf_url":null,"source":{"id":"https://openalex.org/S62072220","display_name":"Journal of Forensic Sciences","issn_l":"0022-1198","issn":["0022-1198","1556-4029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Forensic Sciences","raw_type":"journal-article"},{"id":"pmid:37779342","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/37779342","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of forensic sciences","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1111/1556-4029.15371","doi":"https://doi.org/10.1111/1556-4029.15371","is_oa":true,"landing_page_url":"http://dx.doi.org/10.1111/1556-4029.15371","pdf_url":null,"source":{"id":"https://openalex.org/S62072220","display_name":"Journal of Forensic Sciences","issn_l":"0022-1198","issn":["0022-1198","1556-4029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Forensic Sciences","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":31,"referenced_works":["https://openalex.org/W7382634","https://openalex.org/W95279883","https://openalex.org/W1415533310","https://openalex.org/W1489452649","https://openalex.org/W1516429022","https://openalex.org/W1851276725","https://openalex.org/W1922611140","https://openalex.org/W1963815798","https://openalex.org/W1973207608","https://openalex.org/W1989417703","https://openalex.org/W1996057614","https://openalex.org/W2017190184","https://openalex.org/W2017762517","https://openalex.org/W2017825094","https://openalex.org/W2019955688","https://openalex.org/W2026800686","https://openalex.org/W2030431092","https://openalex.org/W2033554233","https://openalex.org/W2034009934","https://openalex.org/W2040201266","https://openalex.org/W2111967882","https://openalex.org/W2170836481","https://openalex.org/W2617243212","https://openalex.org/W2754788475","https://openalex.org/W2790921965","https://openalex.org/W2792597300","https://openalex.org/W2803877986","https://openalex.org/W2808132065","https://openalex.org/W4214494682","https://openalex.org/W4220735733","https://openalex.org/W6628095676"],"related_works":["https://openalex.org/W7018919741","https://openalex.org/W4220735733","https://openalex.org/W3186275971","https://openalex.org/W7047411447","https://openalex.org/W4393901604","https://openalex.org/W6908587870","https://openalex.org/W2887604616","https://openalex.org/W2556043755","https://openalex.org/W2119648058","https://openalex.org/W2615731441"],"abstract_inverted_index":{"While":[0],"efforts":[1],"have":[2],"been":[3,72],"made":[4],"to":[5,24,83,231,243],"reduce":[6],"the":[7,16,25,42,45,76,100,118,164,175,197,208,232],"pervasive":[8],"backlog":[9],"of":[10,27,44,79,102,157,177,189,210],"sexual":[11,106],"assault":[12,107],"evidence":[13],"collection":[14],"kits,":[15],"actual":[17],"laboratory":[18],"process":[19],"remains":[20],"very":[21],"time-consuming":[22],"due":[23],"requirement":[26],"a":[28,62],"differential":[29,234],"lysis":[30,53,65,101,176,235],"step":[31],"before":[32],"DNA":[33,46,249],"purification,":[34],"as":[35,37,122,124,228],"well":[36,123],"intricate":[38],"mixture":[39],"analysis":[40,188],"towards":[41],"end":[43],"workflow.":[47],"Recently,":[48],"an":[49,85,229],"alternative,":[50],"direct-to-amplification":[51],"sperm":[52,183],"method":[54,66,87,199,225,236],"(using":[55],"1":[56],"M":[57],"NaOH)":[58,168],"was":[59,82,226],"identified.":[60],"However,":[61],"direct":[63],"cell":[64,184],"for":[67,99,174,237],"non-sperm":[68,103,238],"cells":[69,104,180,239],"has":[70],"not":[71,94],"identified":[73],"yet.":[74],"Thus,":[75,223],"primary":[77],"objective":[78],"this":[80,110,224],"work":[81],"find":[84],"alternative":[86,126,230],"that":[88,163,196],"is":[89],"quick,":[90],"inexpensive,":[91],"and":[92,150,159,169,220],"does":[93],"require":[95],"multiple":[96],"purification":[97,250],"steps":[98],"in":[105],"samples.":[108],"In":[109],"study,":[111],"vaginal":[112,158,178,193],"swab":[113],"samples":[114],"were":[115,172],"lysed":[116],"with":[117,130],"control":[119],"method,":[120],"prepGEM\u2122,":[121],"six":[125],"reagents:":[127],"alkaline":[128,165],"buffer":[129],"25-200":[131],"mM":[132,167],"NaOH,":[133],"high-salt":[134],"stain":[135],"extraction":[136,145],"buffer,":[137,142,149],"modified":[138],"radioimmunoprecipitation":[139],"assay":[140],"(RIPA)":[141],"mammalian":[143],"protein":[144],"reagent":[146],"(M-PER\u2122),":[147],"digitonin":[148],"urea/thiourea":[151],"buffer.":[152],"Quantification":[153],"using":[154],"Quantifiler\u00ae":[155],"Trio":[156],"semen":[160],"lysates":[161,194,246],"revealed":[162,195],"(25":[166],"M-PER\u2122":[170,198],"methods":[171],"efficient":[173],"epithelial":[179],"without":[181,247],"substantial":[182],"lysis.":[185],"Following":[186],"quantification,":[187],"STR":[190,212],"profiles":[191],"from":[192],"showed":[200],"promising":[201],"results":[202],"across":[203],"all":[204],"metrics":[205],"examined,":[206],"including":[207],"percentage":[209],"detected":[211],"alleles,":[213],"mean":[214],"peak":[215,217],"heights,":[216],"height":[218],"ratio,":[219],"interlocus":[221],"balance.":[222],"recommended":[227],"traditional":[233],"given":[240],"its":[241],"ability":[242],"produce":[244],"amplification-ready":[245],"any":[248],"step.":[251]},"counts_by_year":[{"year":2026,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2023-10-03T00:00:00"}
