{"id":"https://openalex.org/W4387079973","doi":"https://doi.org/10.1093/clinchem/hvad097.598","title":"B-276 Preservation of Proteomic Inflammatory Biomarkers signal in a Saliva Multiomic Buffer at Room Temperature","display_name":"B-276 Preservation of Proteomic Inflammatory Biomarkers signal in a Saliva Multiomic Buffer at Room Temperature","publication_year":2023,"publication_date":"2023-09-27","ids":{"openalex":"https://openalex.org/W4387079973","doi":"https://doi.org/10.1093/clinchem/hvad097.598"},"language":"en","primary_location":{"id":"doi:10.1093/clinchem/hvad097.598","doi":"https://doi.org/10.1093/clinchem/hvad097.598","is_oa":true,"landing_page_url":"https://doi.org/10.1093/clinchem/hvad097.598","pdf_url":"https://academic.oup.com/clinchem/article-pdf/69/Supplement_1/hvad097.598/51776360/hvad097.598.pdf","source":{"id":"https://openalex.org/S139212996","display_name":"Clinical Chemistry","issn_l":"0009-9147","issn":["0009-9147","1530-8561"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320272","host_organization_name":"American Association for Clinical Chemistry","host_organization_lineage":["https://openalex.org/P4310320272"],"host_organization_lineage_names":["American Association for Clinical Chemistry"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Clinical Chemistry","raw_type":"journal-article"},"volume":null,"type":"conference-abstract","indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://academic.oup.com/clinchem/article-pdf/69/Supplement_1/hvad097.598/51776360/hvad097.598.pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"M M Jensen","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"M M Jensen","raw_affiliation_strings":["Spectrum Solutions, Inc. , Draper, UT"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Spectrum Solutions, Inc. , Draper, UT","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068399737","display_name":"Holden Terry Maecker","orcid":"https://orcid.org/0000-0003-0795-9946","observed_orcids":["https://orcid.org/0000-0003-0795-9946","https://orcid.org/0000-0001-5389-0906"]},"institutions":[{"id":"https://openalex.org/I97018004","display_name":"Stanford University","ror":"https://ror.org/00f54p054","country_code":"US","type":"education","lineage":["https://openalex.org/I97018004"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"H Maecker","raw_affiliation_strings":["Stanford University , Stanford, CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Stanford University , Stanford, CA","institution_ids":["https://openalex.org/I97018004"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110156475","display_name":"TuDung T. Nguyen","orcid":"https://orcid.org/0000-0002-8988-4003","observed_orcids":["https://orcid.org/0000-0002-8988-4003"]},"institutions":[{"id":"https://openalex.org/I97018004","display_name":"Stanford University","ror":"https://ror.org/00f54p054","country_code":"US","type":"education","lineage":["https://openalex.org/I97018004"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"T Nguyen","raw_affiliation_strings":["Stanford University , Palo Alto, CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Stanford University , Palo Alto, CA","institution_ids":["https://openalex.org/I97018004"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113057683","display_name":"\u0110\u1eb7ng Ho\u00e0ng L\u00ea V\u0169","orcid":"https://orcid.org/0009-0001-0589-1717","observed_orcids":["https://orcid.org/0009-0001-0589-1717"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"D Vu","raw_affiliation_strings":["Spectrum Solutions, LLC. , Draper, UT"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Spectrum Solutions, LLC. , Draper, UT","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5040454489","display_name":"David John Vigerust","orcid":"https://orcid.org/0000-0002-8884-2936","observed_orcids":["https://orcid.org/0000-0002-8884-2936"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"D Vigerust","raw_affiliation_strings":["Spectrum Solutions, LLC. , Draper, UT"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Spectrum Solutions, LLC. , Draper, UT","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5100653806","display_name":"Rohit A Gupta","orcid":"https://orcid.org/0000-0003-2958-1526","observed_orcids":["https://orcid.org/0000-0003-2958-1526"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"R Gupta","raw_affiliation_strings":["Spectrum Solutions, LLC. , Draper, UT"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Spectrum Solutions, LLC. , Draper, UT","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4088,"currency":"USD","value_usd":4088},"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"69","issue":"Supplement_1","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11521","display_name":"Salivary Gland Disorders and Functions","score":0.4253000020980835,"subfield":{"id":"https://openalex.org/subfields/2737","display_name":"Physiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11521","display_name":"Salivary Gland Disorders and Functions","score":0.4253000020980835,"subfield":{"id":"https://openalex.org/subfields/2737","display_name":"Physiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12867","display_name":"Advanced Biosensing Techniques and Applications","score":0.24940000474452972,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11314","display_name":"Clinical Laboratory Practices and Quality Control","score":0.1492999941110611,"subfield":{"id":"https://openalex.org/subfields/2737","display_name":"Physiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/saliva","display_name":"saliva","score":0.7269999980926514},{"id":"https://openalex.org/keywords/inflammatory-markers","display_name":"inflammatory markers","score":0.7390000224113464},{"id":"https://openalex.org/keywords/sample-stability","display_name":"sample stability","score":0.6700000166893005},{"id":"https://openalex.org/keywords/room-temperature-storage","display_name":"room temperature storage","score":0.9580000042915344},{"id":"https://openalex.org/keywords/proximity-extension-assay","display_name":"proximity extension assay","score":0.746999979019165},{"id":"https://openalex.org/keywords/serum-comparison","display_name":"serum comparison","score":0.5509999990463257},{"id":"https://openalex.org/keywords/saliva-proteomics","display_name":"saliva proteomics","score":0.45399999618530273},{"id":"https://openalex.org/keywords/home-sample-collection","display_name":"home sample collection","score":0.5659999847412109},{"id":"https://openalex.org/keywords/non-invasive-diagnosis","display_name":"non-invasive diagnosis","score":0.5400000214576721},{"id":"https://openalex.org/keywords/c-reactive-protein","display_name":"C-reactive protein","score":0.5400000214576721},{"id":"https://openalex.org/keywords/interleukin-6","display_name":"interleukin-6","score":0.546999990940094}],"concepts":[{"id":"https://openalex.org/C504460877","wikidata":"https://www.wikidata.org/wiki/Q155925","display_name":"Saliva","level":2,"score":0.9526760578155518},{"id":"https://openalex.org/C10390740","wikidata":"https://www.wikidata.org/wiki/Q2282401","display_name":"Analyte","level":2,"score":0.6973386406898499},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.5435038805007935},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5198403000831604},{"id":"https://openalex.org/C119128265","wikidata":"https://www.wikidata.org/wiki/Q900165","display_name":"Detection limit","level":2,"score":0.47250425815582275},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.41042670607566833},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.250584214925766},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1093/clinchem/hvad097.598","doi":"https://doi.org/10.1093/clinchem/hvad097.598","is_oa":true,"landing_page_url":"https://doi.org/10.1093/clinchem/hvad097.598","pdf_url":"https://academic.oup.com/clinchem/article-pdf/69/Supplement_1/hvad097.598/51776360/hvad097.598.pdf","source":{"id":"https://openalex.org/S139212996","display_name":"Clinical Chemistry","issn_l":"0009-9147","issn":["0009-9147","1530-8561"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320272","host_organization_name":"American Association for Clinical Chemistry","host_organization_lineage":["https://openalex.org/P4310320272"],"host_organization_lineage_names":["American Association for Clinical Chemistry"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Clinical Chemistry","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.1093/clinchem/hvad097.598","doi":"https://doi.org/10.1093/clinchem/hvad097.598","is_oa":true,"landing_page_url":"https://doi.org/10.1093/clinchem/hvad097.598","pdf_url":"https://academic.oup.com/clinchem/article-pdf/69/Supplement_1/hvad097.598/51776360/hvad097.598.pdf","source":{"id":"https://openalex.org/S139212996","display_name":"Clinical Chemistry","issn_l":"0009-9147","issn":["0009-9147","1530-8561"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320272","host_organization_name":"American Association for Clinical Chemistry","host_organization_lineage":["https://openalex.org/P4310320272"],"host_organization_lineage_names":["American Association for Clinical Chemistry"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Clinical Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.9391000270843506}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4387079973.pdf","grobid_xml":"https://content.openalex.org/works/W4387079973.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2079834447","https://openalex.org/W2145406398","https://openalex.org/W2049855233","https://openalex.org/W3216398503","https://openalex.org/W7120795971","https://openalex.org/W2014076617","https://openalex.org/W2002608544","https://openalex.org/W6958641271","https://openalex.org/W2254328016","https://openalex.org/W2160874728"],"abstract_inverted_index":{"Abstract":[0],"Background":[1],"Saliva":[2,92],"represents":[3],"an":[4],"attractive":[5],"biospecimen":[6],"as":[7],"it":[8],"is":[9],"pain-free,":[10],"simple":[11],"for":[12,200,224,309,318],"at":[13,51,189,233,270,413],"home":[14],"collections,":[15,68],"and":[16,42,88,162,183,217,263,314,349,387,403],"patients":[17],"can":[18],"provide":[19,25],"multiple":[20],"samples":[21,83,382,394,408],"over":[22,268,327],"time":[23,26,328],"to":[24,39,59,119,141,213,260,266],"resolved":[27],"data.":[28],"However,":[29,273],"few":[30],"diagnostic":[31],"tests":[32],"have":[33],"been":[34],"developed":[35],"using":[36,157],"saliva":[37,55,66,89,211,218,262,338,365,381],"due":[38],"poor":[40],"stability":[41,178,252,276,336],"comparatively":[43],"low":[44],"concentration":[45],"of":[46,49,62,65,77,84,202,227,236,243,253,277,292,298,311,333,345,364,391],"analytes.":[47,80],"Stabilization":[48],"biomarkers":[50,256,379],"ambient":[52],"temperature":[53,191],"in":[54,164,210,229,287,322,325,337,366,380],"would":[56],"enable":[57],"labs":[58],"take":[60],"advantage":[61],"the":[63,71,75,203,225,230,241,250,274,312,334,370],"benefits":[64],"sample":[67],"while":[69],"minimizing":[70],"risks":[72],"posed":[73],"by":[74,257,395,411],"degradation":[76],"already":[78],"scarce":[79],"Methods":[81],"Matched":[82],"neat":[85,261],"serum,":[86],"saliva,":[87],"collected":[90,98,410],"with":[91,109,136,339],"Multi-omic":[93],"Device":[94],"(SMD)":[95],"preservative":[96],"were":[97,180,294],"from":[99],"30":[100],"volunteers":[101],"(15":[102],"male":[103],"&":[104],"15":[105],"female)":[106],"ages":[107],"18\u201364":[108],"no":[110],"known":[111],"morbidities.":[112],"The":[113,124,320,373],"Olink":[114,371],"Inflammation":[115],"panel":[116,231],"was":[117,155,329],"used":[118],"examine":[120],"92":[121,204],"inflammation-related":[122],"proteins.":[123],"method":[125],"utilized":[126],"proximity":[127],"extension":[128],"assay":[129],"technology,":[130],"wherein":[131],"antibody":[132],"probe":[133],"pairs":[134],"reacted":[135],"their":[137],"respective":[138],"target":[139,147],"proteins":[140,286],"generate":[142],"a":[143,168],"polymerase":[144,159],"chain":[145,160],"reaction":[146,161],"sequence":[148,154],"through":[149,384],"proximity-dependent":[150],"DNA":[151],"polymerization.":[152],"This":[153,359],"measured":[156,181],"real-time":[158],"normalized":[163],"two":[165],"steps,":[166],"producing":[167],"semi-quantitative":[169],"Normalized":[170],"Protein":[171,196],"eXpression":[172],"(NPX)":[173,198],"unit.":[174],"To":[175],"evaluate":[176],"analyte":[177],"specimens":[179],"initially":[182],"then":[184],"again":[185],"after":[186],"24":[187,300],"h":[188,301],"room":[190,271],"(23":[192],"\u00b1":[193,347,351,354],"2\u00b0C).":[194],"Results":[195],"expression":[197],"units":[199],"36":[201],"inflammatory":[205,254,378],"markers":[206,228,281],"had":[207,238],"higher":[208],"concentrations":[209,291],"compared":[212,259,265],"serum.":[214],"Serum":[215],"(78/92)":[216],"78%":[219],"(72/92)":[220],"showed":[221],"similar":[222],"coverage":[223],"number":[226],"where":[232],"least":[234],"75%":[235],"subjects":[237,396],"values":[239,299],"exceeding":[240],"limit":[242],"detection":[244],"(LoD).":[245],"SMD":[246,288,374],"preservation":[247,375],"buffer":[248,376],"improved":[249],"average":[251],"protein":[255,280,323],"19.6%":[258],"10.2%":[264],"serum":[267,326],"24-hours":[269],"temperature.":[272],"relative":[275],"individual":[278],"between":[279],"[DV1]":[282],"[HM2]":[283],"varied.":[284],"For":[285],"buffer,":[289],"initial":[290],"analytes":[293,313],"highly":[295],"predictive":[296,332],"[HM3]":[297],"later,":[302],"having":[303,343],"Pearson":[304],"correlation":[305],"coefficients":[306],">":[307],"0.7,":[308],"23":[310],"greater":[315],"than":[316],"0.5":[317],"47.":[319],"change":[321],"marker":[324],"only":[330],"moderately":[331],"protein\u2019s":[335],"or":[340,416],"without":[341,397],"preservative,":[342],"correlations":[344],"0.33":[346],"0.20":[348],"0.47":[350],"0.19":[352],"(mean":[353],"standard":[355],"deviation),":[356],"respectively.":[357],"Conclusion":[358],"study":[360],"demonstrates":[361],"potential":[362],"utility":[363],"proteomic":[367],"applications":[368],"utilizing":[369],"platform.":[372],"stabilized":[377],"likely":[383],"protease":[385],"inhibition":[386],"antimicrobial":[388],"action.":[389],"Preservation":[390],"self-collection":[392],"salivary":[393],"cold":[398],"storage":[399],"enables":[400],"noninvasive":[401],"sampling":[402],"reliable":[404],"measurements":[405],"even":[406],"when":[407],"are":[409],"users":[412],"remote":[414],"locations":[415],"under":[417],"challenging":[418],"conditions.":[419]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2023-09-28T00:00:00"}