{"id":"https://openalex.org/W4386965746","doi":"https://doi.org/10.21926/obm.genet.2303193","title":"Arginine and Histidine-modified Layered Double Hydroxides Facilitate Transgene Expression in Cancer Cells in Vitro","display_name":"Arginine and Histidine-modified Layered Double Hydroxides Facilitate Transgene Expression in Cancer Cells in Vitro","publication_year":2023,"publication_date":"2023-09-21","ids":{"openalex":"https://openalex.org/W4386965746","doi":"https://doi.org/10.21926/obm.genet.2303193"},"language":"en","primary_location":{"id":"doi:10.21926/obm.genet.2303193","doi":"https://doi.org/10.21926/obm.genet.2303193","is_oa":true,"landing_page_url":"https://doi.org/10.21926/obm.genet.2303193","pdf_url":"https://www.lidsen.com/journals/genetics/genetics-07-03-193/obm.genet.2303193.pdf","source":{"id":"https://openalex.org/S4210236586","display_name":"OBM Genetics","issn_l":"2577-5790","issn":["2577-5790"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-20","russia-white-list-4"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"OBM Genetics","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://www.lidsen.com/journals/genetics/genetics-07-03-193/obm.genet.2303193.pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5070536526","display_name":"Nirasha Nundkumar","orcid":"https://orcid.org/0000-0002-6025-6126","observed_orcids":["https://orcid.org/0000-0002-6025-6126"]},"institutions":[{"id":"https://openalex.org/I95023434","display_name":"University of KwaZulu-Natal","ror":"https://ror.org/04qzfn040","country_code":"ZA","type":"education","lineage":["https://openalex.org/I95023434"]}],"countries":["ZA"],"is_corresponding":false,"raw_author_name":"Nirasha Nundkumar","raw_affiliation_strings":[". Nano-Gene and Drug Delivery Group, Discipline of Biochemistry, University of KwaZulu-Natal, Private Bag X54001, Durban, South Africa;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":". Nano-Gene and Drug Delivery Group, Discipline of Biochemistry, University of KwaZulu-Natal, Private Bag X54001, Durban, South Africa;","institution_ids":["https://openalex.org/I95023434"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5021098104","display_name":"Sooboo Singh","orcid":"https://orcid.org/0000-0001-5765-1013","observed_orcids":["https://orcid.org/0000-0001-5765-1013"]},"institutions":[{"id":"https://openalex.org/I95023434","display_name":"University of KwaZulu-Natal","ror":"https://ror.org/04qzfn040","country_code":"ZA","type":"education","lineage":["https://openalex.org/I95023434"]}],"countries":["ZA"],"is_corresponding":false,"raw_author_name":"Sooboo Singh","raw_affiliation_strings":[". School of Chemistry and Physics, University of KwaZulu-Natal, Private Bag X54001, Durban, South Africa;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":". School of Chemistry and Physics, University of KwaZulu-Natal, Private Bag X54001, Durban, South Africa;","institution_ids":["https://openalex.org/I95023434"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5048895807","display_name":"Moganavelli Singh","orcid":"https://orcid.org/0000-0002-9985-6567","observed_orcids":["https://orcid.org/0000-0002-9985-6567"]},"institutions":[{"id":"https://openalex.org/I95023434","display_name":"University of KwaZulu-Natal","ror":"https://ror.org/04qzfn040","country_code":"ZA","type":"education","lineage":["https://openalex.org/I95023434"]}],"countries":["ZA"],"is_corresponding":true,"raw_author_name":"Moganavelli Singh","raw_affiliation_strings":[". Nano-Gene and Drug Delivery Group, Discipline of Biochemistry, University of KwaZulu-Natal, Private Bag X54001, Durban, South Africa;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":". Nano-Gene and Drug Delivery Group, Discipline of Biochemistry, University of KwaZulu-Natal, Private Bag X54001, Durban, South Africa;","institution_ids":["https://openalex.org/I95023434"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5048895807"],"corresponding_institution_ids":["https://openalex.org/I95023434"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.07578078,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"07","issue":"03","first_page":"1","last_page":"21"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11940","display_name":"Layered Double Hydroxides Synthesis and Applications","score":0.9961000084877014,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11940","display_name":"Layered Double Hydroxides Synthesis and Applications","score":0.9961000084877014,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.002899999963119626,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10063","display_name":"Nanoparticle-Based Drug Delivery","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2502","display_name":"Biomaterials"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/layered-double-hydroxides","display_name":"layered double hydroxides","score":1.0},{"id":"https://openalex.org/keywords/histidine-functionalization","display_name":"histidine functionalization","score":1.0},{"id":"https://openalex.org/keywords/transfection","display_name":"transfection","score":0.6349999904632568},{"id":"https://openalex.org/keywords/transgene-expression","display_name":"transgene expression","score":0.7139999866485596},{"id":"https://openalex.org/keywords/dna-delivery","display_name":"DNA delivery","score":0.7350000143051147},{"id":"https://openalex.org/keywords/proton-sponge-effect","display_name":"proton sponge effect","score":0.7409999966621399},{"id":"https://openalex.org/keywords/cytotoxicity","display_name":"cytotoxicity","score":0.7080000042915344},{"id":"https://openalex.org/keywords/colorectal-cancer-cells","display_name":"colorectal cancer cells","score":0.8059999942779541},{"id":"https://openalex.org/keywords/hepatocellular-carcinoma-cells","display_name":"hepatocellular carcinoma cells","score":0.9800000190734863},{"id":"https://openalex.org/keywords/hek293-cells","display_name":"HEK293 cells","score":0.8949999809265137},{"id":"https://openalex.org/keywords/co-precipitation-synthesis","display_name":"co-precipitation synthesis","score":0.7409999966621399}],"concepts":[{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.7971231341362},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.6993430852890015},{"id":"https://openalex.org/C174510640","wikidata":"https://www.wikidata.org/wiki/Q489618","display_name":"HEK 293 cells","level":3,"score":0.5706323385238647},{"id":"https://openalex.org/C102230213","wikidata":"https://www.wikidata.org/wiki/Q430117","display_name":"Transgene","level":3,"score":0.49632006883621216},{"id":"https://openalex.org/C161733203","wikidata":"https://www.wikidata.org/wiki/Q911828","display_name":"Reporter gene","level":4,"score":0.4795953333377838},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4679884612560272},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.4560319483280182},{"id":"https://openalex.org/C109316439","wikidata":"https://www.wikidata.org/wiki/Q246181","display_name":"Cytotoxicity","level":3,"score":0.42878082394599915},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.4115554094314575},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.39209312200546265},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3194424510002136},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.2665712833404541},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.2106202244758606}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.21926/obm.genet.2303193","doi":"https://doi.org/10.21926/obm.genet.2303193","is_oa":true,"landing_page_url":"https://doi.org/10.21926/obm.genet.2303193","pdf_url":"https://www.lidsen.com/journals/genetics/genetics-07-03-193/obm.genet.2303193.pdf","source":{"id":"https://openalex.org/S4210236586","display_name":"OBM Genetics","issn_l":"2577-5790","issn":["2577-5790"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-20","russia-white-list-4"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"OBM Genetics","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.21926/obm.genet.2303193","doi":"https://doi.org/10.21926/obm.genet.2303193","is_oa":true,"landing_page_url":"https://doi.org/10.21926/obm.genet.2303193","pdf_url":"https://www.lidsen.com/journals/genetics/genetics-07-03-193/obm.genet.2303193.pdf","source":{"id":"https://openalex.org/S4210236586","display_name":"OBM Genetics","issn_l":"2577-5790","issn":["2577-5790"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-20","russia-white-list-4"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"OBM Genetics","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.428600013256073,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G882756792","display_name":null,"funder_award_id":"129263","funder_id":"https://openalex.org/F4320320671","funder_display_name":"National Research Foundation"}],"funders":[{"id":"https://openalex.org/F4320320671","display_name":"National Research Foundation","ror":"https://ror.org/05s0g1g46"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4386965746.pdf","grobid_xml":"https://content.openalex.org/works/W4386965746.grobid-xml"},"referenced_works_count":65,"referenced_works":["https://openalex.org/W1879047861","https://openalex.org/W1968635440","https://openalex.org/W1973685759","https://openalex.org/W1976456910","https://openalex.org/W1985068700","https://openalex.org/W1988985810","https://openalex.org/W1994677316","https://openalex.org/W2007853971","https://openalex.org/W2018032428","https://openalex.org/W2022093429","https://openalex.org/W2023013902","https://openalex.org/W2033655482","https://openalex.org/W2034518767","https://openalex.org/W2034765817","https://openalex.org/W2043978343","https://openalex.org/W2047522840","https://openalex.org/W2070521942","https://openalex.org/W2074118084","https://openalex.org/W2082961526","https://openalex.org/W2083911023","https://openalex.org/W2095717318","https://openalex.org/W2107385066","https://openalex.org/W2113974943","https://openalex.org/W2143030510","https://openalex.org/W2144282259","https://openalex.org/W2150940207","https://openalex.org/W2170355606","https://openalex.org/W2184943267","https://openalex.org/W2285785821","https://openalex.org/W2313741836","https://openalex.org/W2396380901","https://openalex.org/W2503926928","https://openalex.org/W2547437674","https://openalex.org/W2555194424","https://openalex.org/W2578455328","https://openalex.org/W2608314580","https://openalex.org/W2742244542","https://openalex.org/W2779000621","https://openalex.org/W2899657088","https://openalex.org/W2979544071","https://openalex.org/W2989809587","https://openalex.org/W2996946223","https://openalex.org/W3006316590","https://openalex.org/W3012896294","https://openalex.org/W3016998270","https://openalex.org/W3021948958","https://openalex.org/W3031866582","https://openalex.org/W3091445330","https://openalex.org/W3110781745","https://openalex.org/W3128726783","https://openalex.org/W3134804485","https://openalex.org/W3159968517","https://openalex.org/W3165750686","https://openalex.org/W3217273851","https://openalex.org/W4200508040","https://openalex.org/W4205465531","https://openalex.org/W4210345463","https://openalex.org/W4224937271","https://openalex.org/W4234509136","https://openalex.org/W4285682553","https://openalex.org/W4292396183","https://openalex.org/W4318043130","https://openalex.org/W4318952398","https://openalex.org/W4363674703","https://openalex.org/W4377290249"],"related_works":["https://openalex.org/W1985068700","https://openalex.org/W2139691539","https://openalex.org/W1967320666","https://openalex.org/W2332530935","https://openalex.org/W45238732","https://openalex.org/W2003996743","https://openalex.org/W2301428225","https://openalex.org/W2793900936","https://openalex.org/W2046759961","https://openalex.org/W7116788432"],"abstract_inverted_index":{"Layered":[0],"double":[1],"hydroxides":[2],"(LDHs)":[3],"have":[4,211],"interesting":[5],"properties":[6],"and":[7,29,37,60,85,96,110,125,184,188,218],"structures":[8],"that":[9,180],"enable":[10],"them":[11],"to":[12,40,122,215],"carry":[13],"nucleic":[14],"acids,":[15],"such":[16],"as":[17],"deoxyribonucleic":[18],"acid":[19],"(DNA).":[20],"This":[21],"study":[22],"synthesized":[23],"LDHs":[24,49,69,174,210],"using":[25,52,80,90],"the":[26,32,68,71,91,102,126,133,137,145,160,164,168,182,192,200,206,213],"co-precipitation":[27],"method":[28],"functionalized":[30],"with":[31,70,205],"amino":[33],"acids":[34],"arginine":[35],"(Arg)":[36],"histidine":[38],"(His)":[39],"promote":[41],"proton-sponge":[42],"activity":[43],"for":[44],"enhanced":[45],"transgene":[46,86,128],"expression.":[47],"The":[48,65,115,148,172],"were":[50,119],"characterized":[51],"X-ray":[53],"diffraction":[54],"(XRD),":[55],"transmission":[56],"electron":[57],"microscopy":[58],"(TEM),":[59],"nanoparticle":[61],"tracking":[62],"analysis":[63],"(NTA).":[64],"interaction":[66],"of":[67,141,181],"reporter":[72,98],"gene":[73,99],"plasmid":[74],"DNA":[75],"(pCMV-Luc":[76],"DNA)":[77],"was":[78,88,130,197],"determined":[79],"agarose":[81],"gel":[82],"electrophoresis.":[83],"Cytotoxicity":[84],"expression":[87,129],"assessed":[89],"3-(4,":[92],"5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium":[93],"bromide":[94],"(MTT)":[95],"luciferase":[97],"assay":[100],"in":[101,132,157,163,191,199,222],"human":[103],"embryonic":[104],"kidney":[105],"(HEK293),":[106],"colorectal":[107],"carcinoma":[108,112],"(Caco-2)":[109],"hepatocellular":[111],"(HepG2)":[113],"cells.":[114,147,194],"DNA:":[116],"LDH":[117],"complexes":[118,150],"relatively":[120],"non-cytotoxic":[121],"all":[123],"cells,":[124,202],"highest":[127],"achieved":[131],"HEK293":[134,165],"cells":[135,166],"exhibiting":[136],"most":[138],"significant":[139],"degree":[140],"transfection,":[142,177],"followed":[143],"by":[144,186],"Caco-2":[146,193],"His-LDH":[149,183],"displayed":[151],"more":[152],"than":[153,159],"a":[154],"two-fold":[155],"increase":[156],"transfection":[158],"Arg-LDHs,":[161],"especially":[162],"at":[167],"optimal":[169],"binding":[170],"ratio.":[171],"non-functionalized":[173],"demonstrated":[175,212],"high":[176],"which":[178,203],"exceeded":[179],"Arg-LDH":[185],"20%":[187],"30%,":[189],"respectively,":[190],"Little":[195],"difference":[196],"noted":[198],"HepG2":[201],"presented":[204],"lowest":[207],"transfection.":[208],"These":[209],"potential":[214],"bind,":[216],"protect,":[217],"efficiently":[219],"deliver":[220],"pDNA":[221],"vitro.":[223]},"counts_by_year":[],"updated_date":"2026-10-30T12:39:25.321706","created_date":"2023-09-23T00:00:00"}
