{"id":"https://openalex.org/W4386590251","doi":"https://doi.org/10.1002/pro.4779","title":"Enantioselectivity in the enzymatic dehydration of malate and tartrate: Mirror image specificities of structurally similar dehydratases","display_name":"Enantioselectivity in the enzymatic dehydration of malate and tartrate: Mirror image specificities of structurally similar dehydratases","publication_year":2023,"publication_date":"2023-09-11","ids":{"openalex":"https://openalex.org/W4386590251","doi":"https://doi.org/10.1002/pro.4779","pmid":"https://pubmed.ncbi.nlm.nih.gov/37695939","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/10535810"},"language":"en","primary_location":{"id":"doi:10.1002/pro.4779","doi":"https://doi.org/10.1002/pro.4779","is_oa":false,"landing_page_url":"https://doi.org/10.1002/pro.4779","pdf_url":null,"source":{"id":"https://openalex.org/S156919612","display_name":"Protein Science","issn_l":"0961-8368","issn":["0961-8368","1469-896X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein Science","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2023/06/17/2023.06.17.545066.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5015900710","display_name":"Asutosh Bellur","orcid":"https://orcid.org/0000-0002-5366-0630","observed_orcids":["https://orcid.org/0000-0002-5366-0630"]},"institutions":[{"id":"https://openalex.org/I147481903","display_name":"Jawaharlal Nehru Centre for Advanced Scientific Research","ror":"https://ror.org/0538gdx71","country_code":"IN","type":"facility","lineage":["https://openalex.org/I147481903","https://openalex.org/I2799351866","https://openalex.org/I4210098748","https://openalex.org/I4210134808"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Asutosh Bellur","raw_affiliation_strings":["Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India"],"raw_orcid":"https://orcid.org/0000-0002-5366-0630","affiliations":[{"raw_affiliation_string":"Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India","institution_ids":["https://openalex.org/I147481903"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5073293774","display_name":"Souradip Mukherjee","orcid":"https://orcid.org/0000-0002-7037-1996","observed_orcids":["https://orcid.org/0000-0002-7037-1996"]},"institutions":[{"id":"https://openalex.org/I147481903","display_name":"Jawaharlal Nehru Centre for Advanced Scientific Research","ror":"https://ror.org/0538gdx71","country_code":"IN","type":"facility","lineage":["https://openalex.org/I147481903","https://openalex.org/I2799351866","https://openalex.org/I4210098748","https://openalex.org/I4210134808"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Souradip Mukherjee","raw_affiliation_strings":["Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India"],"raw_orcid":"https://orcid.org/0000-0002-7037-1996","affiliations":[{"raw_affiliation_string":"Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India","institution_ids":["https://openalex.org/I147481903"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5084882023","display_name":"Pragya Niraj Sharma","orcid":"https://orcid.org/0000-0003-4492-2720","observed_orcids":["https://orcid.org/0000-0003-4492-2720"]},"institutions":[{"id":"https://openalex.org/I147481903","display_name":"Jawaharlal Nehru Centre for Advanced Scientific Research","ror":"https://ror.org/0538gdx71","country_code":"IN","type":"facility","lineage":["https://openalex.org/I147481903","https://openalex.org/I2799351866","https://openalex.org/I4210098748","https://openalex.org/I4210134808"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Pragya Sharma","raw_affiliation_strings":["Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India"],"raw_orcid":"https://orcid.org/0000-0003-4492-2720","affiliations":[{"raw_affiliation_string":"Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India","institution_ids":["https://openalex.org/I147481903"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5011470213","display_name":"Vijay Jayaraman","orcid":"https://orcid.org/0000-0001-6288-1841","observed_orcids":["https://orcid.org/0000-0001-6288-1841"]},"institutions":[{"id":"https://openalex.org/I147481903","display_name":"Jawaharlal Nehru Centre for Advanced Scientific Research","ror":"https://ror.org/0538gdx71","country_code":"IN","type":"facility","lineage":["https://openalex.org/I147481903","https://openalex.org/I2799351866","https://openalex.org/I4210098748","https://openalex.org/I4210134808"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Vijay Jayaraman","raw_affiliation_strings":["Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India"],"raw_orcid":"https://orcid.org/0000-0001-6288-1841","affiliations":[{"raw_affiliation_string":"Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India","institution_ids":["https://openalex.org/I147481903"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5024273182","display_name":"Hemalatha Balaram","orcid":"https://orcid.org/0000-0002-8821-1288","observed_orcids":["https://orcid.org/0000-0002-8821-1288"]},"institutions":[{"id":"https://openalex.org/I147481903","display_name":"Jawaharlal Nehru Centre for Advanced Scientific Research","ror":"https://ror.org/0538gdx71","country_code":"IN","type":"facility","lineage":["https://openalex.org/I147481903","https://openalex.org/I2799351866","https://openalex.org/I4210098748","https://openalex.org/I4210134808"]}],"countries":["IN"],"is_corresponding":true,"raw_author_name":"Hemalatha Balaram","raw_affiliation_strings":["Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India"],"raw_orcid":"https://orcid.org/0000-0002-8821-1288","affiliations":[{"raw_affiliation_string":"Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India","institution_ids":["https://openalex.org/I147481903"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5024273182"],"corresponding_institution_ids":["https://openalex.org/I147481903"],"apc_list":{"value":4070,"currency":"USD","value_usd":4070},"apc_paid":null,"fwci":0.102,"has_fulltext":true,"cited_by_count":1,"citation_normalized_percentile":{"value":0.40101738,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":93},"biblio":{"volume":"32","issue":"10","first_page":"e4779","last_page":"e4779"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.7333999872207642,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.7333999872207642,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.08560000360012054,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12191","display_name":"Physical Organic Chemistry and Reaction Kinetics","score":0.038100000470876694,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/enantioselectivity","display_name":"enantioselectivity","score":0.9399999976158142},{"id":"https://openalex.org/keywords/malate-dehydrogenase","display_name":"malate dehydrogenase","score":0.8180000185966492},{"id":"https://openalex.org/keywords/fumarate-hydratase","display_name":"fumarate hydratase","score":0.8090000152587891},{"id":"https://openalex.org/keywords/hydrogen-abstraction","display_name":"hydrogen abstraction","score":0.46799999475479126},{"id":"https://openalex.org/keywords/methanocaldococcus-jannaschii","display_name":"Methanococcus jannaschii","score":0.8650000095367432},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.9950000047683716},{"id":"https://openalex.org/keywords/enzyme-evolution","display_name":"enzyme evolution","score":0.5649999976158142},{"id":"https://openalex.org/keywords/conformational-plasticity","display_name":"conformational plasticity","score":0.8849999904632568},{"id":"https://openalex.org/keywords/active-site-residues","display_name":"active site residues","score":0.4230000078678131}],"concepts":[{"id":"https://openalex.org/C187921627","wikidata":"https://www.wikidata.org/wiki/Q2469470","display_name":"Stereospecificity","level":3,"score":0.6916248798370361},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.6641876697540283},{"id":"https://openalex.org/C2781374117","wikidata":"https://www.wikidata.org/wiki/Q5252382","display_name":"Dehydratase","level":3,"score":0.5216034650802612},{"id":"https://openalex.org/C132901563","wikidata":"https://www.wikidata.org/wiki/Q412007","display_name":"Malate dehydrogenase","level":3,"score":0.5078516602516174},{"id":"https://openalex.org/C2777699559","wikidata":"https://www.wikidata.org/wiki/Q425046","display_name":"Tartrate","level":2,"score":0.5035967230796814},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5021069049835205},{"id":"https://openalex.org/C486523","wikidata":"https://www.wikidata.org/wiki/Q494483","display_name":"Enantiomer","level":2,"score":0.4946880042552948},{"id":"https://openalex.org/C41183919","wikidata":"https://www.wikidata.org/wiki/Q423026","display_name":"Active site","level":3,"score":0.4714948534965515},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.4472865164279938},{"id":"https://openalex.org/C2777269803","wikidata":"https://www.wikidata.org/wiki/Q407727","display_name":"Lyase","level":3,"score":0.44215893745422363},{"id":"https://openalex.org/C2777289219","wikidata":"https://www.wikidata.org/wiki/Q7632154","display_name":"Substrate (aquarium)","level":2,"score":0.4322407841682434},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3226752281188965},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2507760524749756},{"id":"https://openalex.org/C161790260","wikidata":"https://www.wikidata.org/wiki/Q82264","display_name":"Catalysis","level":2,"score":0.0},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D003681","descriptor_name":"Dehydration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005649","descriptor_name":"Fumarate Hydratase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D005649","descriptor_name":"Fumarate Hydratase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005649","descriptor_name":"Fumarate Hydratase","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006836","descriptor_name":"Hydro-Lyases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D006836","descriptor_name":"Hydro-Lyases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008293","descriptor_name":"Malates","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010802","descriptor_name":"Phylogeny","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013644","descriptor_name":"Tartrates","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D020134","descriptor_name":"Catalytic Domain","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1002/pro.4779","doi":"https://doi.org/10.1002/pro.4779","is_oa":false,"landing_page_url":"https://doi.org/10.1002/pro.4779","pdf_url":null,"source":{"id":"https://openalex.org/S156919612","display_name":"Protein Science","issn_l":"0961-8368","issn":["0961-8368","1469-896X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein Science","raw_type":"journal-article"},{"id":"doi:10.1101/2023.06.17.545066","doi":"https://doi.org/10.1101/2023.06.17.545066","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2023.06.17.545066","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2023/06/17/2023.06.17.545066.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},{"id":"pmh:oai:pubmedcentral.nih.gov:10535810","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/10535810","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC10535810/pdf/PRO-32-e4779.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein Sci","raw_type":"Text"},{"id":"pmid:37695939","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/37695939","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein science : a publication of the Protein Society","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1101/2023.06.17.545066","doi":"https://doi.org/10.1101/2023.06.17.545066","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2023.06.17.545066","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2023/06/17/2023.06.17.545066.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1492359745","display_name":null,"funder_award_id":"BT/PR11294/BRB/10/1291/2014","funder_id":"https://openalex.org/F4320323128","funder_display_name":"Jawaharlal Nehru Centre for Advanced Scientific Research"},{"id":"https://openalex.org/G5640629315","display_name":null,"funder_award_id":"BT/PR11294/BRB/10/1291/2014","funder_id":"https://openalex.org/F4320334771","funder_display_name":"Science and Engineering Research Board"},{"id":"https://openalex.org/G8327785227","display_name":null,"funder_award_id":"BT/PR13760/COE/34/42/2015","funder_id":"https://openalex.org/F4320334771","funder_display_name":"Science and Engineering Research Board"},{"id":"https://openalex.org/G8425137865","display_name":null,"funder_award_id":"CRG/2019/004150/IBS","funder_id":"https://openalex.org/F4320323128","funder_display_name":"Jawaharlal Nehru Centre for Advanced Scientific Research"},{"id":"https://openalex.org/G8461130411","display_name":null,"funder_award_id":"BT/PR13760/COE/34/42/2015","funder_id":"https://openalex.org/F4320323128","funder_display_name":"Jawaharlal Nehru Centre for Advanced Scientific Research"},{"id":"https://openalex.org/G902388150","display_name":null,"funder_award_id":"CRG/2019/004150/IBS","funder_id":"https://openalex.org/F4320334771","funder_display_name":"Science and Engineering Research Board"}],"funders":[{"id":"https://openalex.org/F4320323128","display_name":"Jawaharlal Nehru Centre for Advanced Scientific Research","ror":"https://ror.org/0538gdx71"},{"id":"https://openalex.org/F4320334771","display_name":"Science and Engineering Research Board","ror":"https://ror.org/03ffdsr55"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4386590251.pdf","grobid_xml":"https://content.openalex.org/works/W4386590251.grobid-xml"},"referenced_works_count":29,"referenced_works":["https://openalex.org/W255996521","https://openalex.org/W1895528264","https://openalex.org/W1990061268","https://openalex.org/W1990224324","https://openalex.org/W2005973569","https://openalex.org/W2010004274","https://openalex.org/W2013279599","https://openalex.org/W2022146097","https://openalex.org/W2027266940","https://openalex.org/W2030966943","https://openalex.org/W2048071308","https://openalex.org/W2082232204","https://openalex.org/W2106121486","https://openalex.org/W2127322768","https://openalex.org/W2128635872","https://openalex.org/W2138604855","https://openalex.org/W2138606219","https://openalex.org/W2151625337","https://openalex.org/W2168974130","https://openalex.org/W2169150541","https://openalex.org/W2294300376","https://openalex.org/W2511929142","https://openalex.org/W2799524357","https://openalex.org/W2952306726","https://openalex.org/W3012317413","https://openalex.org/W3177828909","https://openalex.org/W3211795435","https://openalex.org/W4313455180","https://openalex.org/W4315707290"],"related_works":["https://openalex.org/W1581689919","https://openalex.org/W1986408552","https://openalex.org/W2091527391","https://openalex.org/W2058834319","https://openalex.org/W4205384157","https://openalex.org/W2119654699","https://openalex.org/W7170791077","https://openalex.org/W4391752069","https://openalex.org/W2016018777","https://openalex.org/W2145465660"],"abstract_inverted_index":{"Malate":[0],"(2-hydroxysuccinic":[1],"acid)":[2,6],"and":[3,18,29,36,47,54,80,89,104,117,162,191],"tartrate":[4],"(2,3-dihydroxysuccinic":[5],"are":[7,58,209],"chiral":[8],"substrates;":[9],"the":[10,21,39,43,65,94,131,139,180,185,195,199,216,226,233],"former":[11],"existing":[12],"in":[13,133,150,169,225,235],"two":[14,59],"enantiomeric":[15],"forms":[16],"(R":[17],"S)":[19],"while":[20,93],"latter":[22],"exists":[23],"as":[24,91,106],"three":[25],"stereoisomers":[26,77],"(R,R;":[27],"S,S;":[28],"R,S).":[30],"Dehydration":[31],"by":[32,175],"stereospecific":[33],"hydrogen":[34],"abstraction":[35],"antielimination":[37],"of":[38,70,78,115,142,157,160,165,179,198,206],"hydroxyl":[40],"group":[41],"yield":[42],"achiral":[44],"products":[45],"fumarate":[46,51],"oxaloacetate,":[48],"respectively.":[49],"Class-I":[50],"hydratase":[52],"(FH)":[53],"L-tartrate":[55],"dehydratase":[56],"(L-TTD)":[57],"highly":[60],"conserved":[61],"enzymes":[62,71],"belonging":[63],"to":[64,130,138,211,232],"iron-sulfur":[66],"cluster":[67],"hydrolyase":[68],"family":[69],"that":[72,126,221],"catalyze":[73],"reactions":[74],"on":[75],"specific":[76],"malate":[79],"tartrate.":[81],"FH":[82,161],"from":[83,98,155],"Methanocaldococcus":[84],"jannaschii":[85],"accepts":[86,101],"only":[87,102],"(S)-malate":[88],"(S,S)-tartrate":[90],"substrates":[92],"structurally":[95],"similar":[96],"L-TTD":[97],"Escherichia":[99],"coli":[100],"(R)-malate":[103],"(R,R)-tartrate":[105],"substrates.":[107,202],"Phylogenetic":[108],"analysis":[109,156],"reveals":[110],"a":[111,122,145,214,222],"common":[112],"evolutionary":[113],"origin":[114],"L-TTDs":[116],"two-subunit":[118],"archaeal":[119],"FHs":[120],"suggesting":[121],"divergence":[123],"during":[124],"evolution":[125],"may":[127,172],"have":[128],"led":[129],"switch":[132,149,168,234],"substrate":[134,189,236],"stereospecificity":[135,151],"preference.":[136],"Due":[137],"high":[140],"conservation":[141],"their":[143],"sequences,":[144],"molecular":[146],"basis":[147],"for":[148],"is":[152],"not":[153],"evident":[154],"crystal":[158],"structures":[159],"predicted":[163],"structure":[164],"L-TTD.":[166],"The":[167],"enantiomer":[170,217],"preference":[171],"be":[173],"rationalized":[174],"invoking":[176],"conformational":[177],"plasticity":[178],"amino":[181],"acids":[182],"interacting":[183],"with":[184,188],"substrate,":[186],"together":[187],"reorientation":[190,224],"conformer":[192],"selection":[193],"about":[194],"C2\uf8ffC3":[196],"bond":[197],"dicarboxylic":[200],"acid":[201],"Although":[203],"classical":[204],"models":[205],"enzyme-substrate":[207],"binding":[208],"insufficient":[210],"explain":[212],"such":[213],"phenomenon,":[215],"superposition":[218],"model":[219],"suggests":[220],"minor":[223],"active":[227],"site":[228],"residues":[229],"could":[230],"lead":[231],"stereospecificity.":[237]},"counts_by_year":[{"year":2025,"cited_by_count":1}],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2023-09-11T00:00:00"}