{"id":"https://openalex.org/W4386088091","doi":"https://doi.org/10.1063/5.0161094","title":"Methodology of development and approbation of a test system for identification of Penicillium expansum based on polymerase chain reaction (real-time)","display_name":"Methodology of development and approbation of a test system for identification of Penicillium expansum based on polymerase chain reaction (real-time)","publication_year":2023,"publication_date":"2023-01-01","ids":{"openalex":"https://openalex.org/W4386088091","doi":"https://doi.org/10.1063/5.0161094"},"language":"en","primary_location":{"id":"doi:10.1063/5.0161094","doi":"https://doi.org/10.1063/5.0161094","is_oa":false,"landing_page_url":"http://dx.doi.org/10.1063/5.0161094","pdf_url":null,"source":{"id":"https://openalex.org/S2764696622","display_name":"AIP conference proceedings","issn_l":"0094-243X","issn":["0094-243X","1551-7616","1935-0465"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","norway-1"],"host_organization":"https://openalex.org/P4310320257","host_organization_name":"American Institute of Physics","host_organization_lineage":["https://openalex.org/P4310320257"],"host_organization_lineage_names":["American Institute of Physics"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"AIP Conference Proceedings","raw_type":"proceedings-article"},"volume":null,"type":"conference-paper","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5104159572","display_name":"Natalya Feoktistova","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210162777","display_name":"Ulyanovsk State Agricultural Academy named after PA Stolypin","ror":"https://ror.org/05ssws785","country_code":"RU","type":"education","lineage":["https://openalex.org/I4210162777"]}],"countries":["RU"],"is_corresponding":true,"raw_author_name":"Natalya Feoktistova","raw_affiliation_strings":["Ulyanovsk state agrarian university named after P.\u0410. Stolypin, 1, Novy Venets boulevard, Ulyanovsk, 432017, Russian Federation"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Ulyanovsk state agrarian university named after P.\u0410. Stolypin, 1, Novy Venets boulevard, Ulyanovsk, 432017, Russian Federation","institution_ids":["https://openalex.org/I4210162777"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5070309635","display_name":"\u0421\u0443\u043b\u044c\u0434\u0438\u043d\u0430 \u0415\u043a\u0430\u0442\u0435\u0440\u0438\u043d\u0430 \u0412\u043b\u0430\u0434\u0438\u043c\u0438\u0440\u043e\u0432\u043d\u0430","orcid":"https://orcid.org/0000-0003-3311-1498","observed_orcids":["https://orcid.org/0000-0003-3311-1498"]},"institutions":[{"id":"https://openalex.org/I4210162777","display_name":"Ulyanovsk State Agricultural Academy named after PA Stolypin","ror":"https://ror.org/05ssws785","country_code":"RU","type":"education","lineage":["https://openalex.org/I4210162777"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"Ekaterina Suldina","raw_affiliation_strings":["Ulyanovsk state agrarian university named after P.\u0410. Stolypin, 1, Novy Venets boulevard, Ulyanovsk, 432017, Russian Federation"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Ulyanovsk state agrarian university named after P.\u0410. Stolypin, 1, Novy Venets boulevard, Ulyanovsk, 432017, Russian Federation","institution_ids":["https://openalex.org/I4210162777"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5048046805","display_name":"\u041b\u043e\u043c\u0430\u043a\u0438\u043d \u0410\u0440\u0442\u0451\u043c \u0410\u043d\u0434\u0440\u0435\u0435\u0432\u0438\u0447","orcid":"https://orcid.org/0000-0001-9214-7816","observed_orcids":["https://orcid.org/0000-0001-9214-7816"]},"institutions":[{"id":"https://openalex.org/I4210162777","display_name":"Ulyanovsk State Agricultural Academy named after PA Stolypin","ror":"https://ror.org/05ssws785","country_code":"RU","type":"education","lineage":["https://openalex.org/I4210162777"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"Artem Lomakin","raw_affiliation_strings":["Ulyanovsk state agrarian university named after P.\u0410. Stolypin, 1, Novy Venets boulevard, Ulyanovsk, 432017, Russian Federation"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Ulyanovsk state agrarian university named after P.\u0410. Stolypin, 1, Novy Venets boulevard, Ulyanovsk, 432017, Russian Federation","institution_ids":["https://openalex.org/I4210162777"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113851014","display_name":"\u0410.\u0412. \u041c\u0430\u0441\u0442\u0438\u043b\u0435\u043d\u043a\u043e","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210162777","display_name":"Ulyanovsk State Agricultural Academy named after PA Stolypin","ror":"https://ror.org/05ssws785","country_code":"RU","type":"education","lineage":["https://openalex.org/I4210162777"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"Andrey Mastilenko","raw_affiliation_strings":["Ulyanovsk state agrarian university named after P.\u0410. Stolypin, 1, Novy Venets boulevard, Ulyanovsk, 432017, Russian Federation"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Ulyanovsk state agrarian university named after P.\u0410. Stolypin, 1, Novy Venets boulevard, Ulyanovsk, 432017, Russian Federation","institution_ids":["https://openalex.org/I4210162777"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5008120363","display_name":"Ilgizar I. Bogdanov","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210162777","display_name":"Ulyanovsk State Agricultural Academy named after PA Stolypin","ror":"https://ror.org/05ssws785","country_code":"RU","type":"education","lineage":["https://openalex.org/I4210162777"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"Ilgizar Bogdanov","raw_affiliation_strings":["Ulyanovsk state agrarian university named after P.\u0410. Stolypin, 1, Novy Venets boulevard, Ulyanovsk, 432017, Russian Federation"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Ulyanovsk state agrarian university named after P.\u0410. Stolypin, 1, Novy Venets boulevard, Ulyanovsk, 432017, Russian Federation","institution_ids":["https://openalex.org/I4210162777"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5104159572"],"corresponding_institution_ids":["https://openalex.org/I4210162777"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"3011","issue":null,"first_page":"020048","last_page":"020048"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10825","display_name":"Plant Pathogens and Fungal Diseases","score":0.8769999742507935,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10825","display_name":"Plant Pathogens and Fungal Diseases","score":0.8769999742507935,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10520","display_name":"Mycotoxins in Agriculture and Food","score":0.0575999990105629,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.02879999950528145,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/penicillium-expansum","display_name":"Penicillium expansum","score":1.0},{"id":"https://openalex.org/keywords/blue-mold","display_name":"blue mold","score":0.9089999794960022},{"id":"https://openalex.org/keywords/patulin-biosynthesis","display_name":"patulin biosynthesis","score":0.718999981880188},{"id":"https://openalex.org/keywords/real-time-pcr","display_name":"real-time PCR","score":0.7170000076293945},{"id":"https://openalex.org/keywords/fungal-identification","display_name":"fungal identification","score":0.6010000109672546},{"id":"https://openalex.org/keywords/mycotoxins","display_name":"mycotoxins","score":0.6200000047683716},{"id":"https://openalex.org/keywords/food-safety","display_name":"food safety","score":0.5289999842643738},{"id":"https://openalex.org/keywords/primer-optimization","display_name":"primer optimization","score":0.3610000014305115}],"concepts":[{"id":"https://openalex.org/C2780630512","wikidata":"https://www.wikidata.org/wiki/Q147358","display_name":"Penicillium expansum","level":3,"score":0.6946840882301331},{"id":"https://openalex.org/C116834253","wikidata":"https://www.wikidata.org/wiki/Q2039217","display_name":"Identification (biology)","level":2,"score":0.5488938093185425},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.46908441185951233},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.44993874430656433},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.13840946555137634},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.12019684910774231},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.10087576508522034},{"id":"https://openalex.org/C157670687","wikidata":"https://www.wikidata.org/wiki/Q426933","display_name":"Postharvest","level":2,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1063/5.0161094","doi":"https://doi.org/10.1063/5.0161094","is_oa":false,"landing_page_url":"http://dx.doi.org/10.1063/5.0161094","pdf_url":null,"source":{"id":"https://openalex.org/S2764696622","display_name":"AIP conference proceedings","issn_l":"0094-243X","issn":["0094-243X","1551-7616","1935-0465"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","norway-1"],"host_organization":"https://openalex.org/P4310320257","host_organization_name":"American Institute of Physics","host_organization_lineage":["https://openalex.org/P4310320257"],"host_organization_lineage_names":["American Institute of Physics"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"AIP Conference Proceedings","raw_type":"proceedings-article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Zero hunger","id":"https://metadata.un.org/sdg/2","score":0.5365999937057495}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":12,"referenced_works":["https://openalex.org/W185429612","https://openalex.org/W1484322271","https://openalex.org/W1993979112","https://openalex.org/W2024960103","https://openalex.org/W2067578233","https://openalex.org/W2093961177","https://openalex.org/W2284577735","https://openalex.org/W2869784560","https://openalex.org/W2914634440","https://openalex.org/W3025891189","https://openalex.org/W3081718261","https://openalex.org/W3088401090"],"related_works":["https://openalex.org/W2080251147","https://openalex.org/W2042962360","https://openalex.org/W2330314131","https://openalex.org/W2388399697","https://openalex.org/W6960714276","https://openalex.org/W4294266513","https://openalex.org/W3099448592","https://openalex.org/W2801791265","https://openalex.org/W2381015066","https://openalex.org/W4378234901"],"abstract_inverted_index":{"The":[0,70,103,147,164,173,239],"article":[1],"shows":[2],"the":[3,8,22,54,65,77,93,168,176,211,234,246,250,255,258,262],"results":[4],"of":[5,10,24,42,67,75,89,95,99,150,156,167,175,185,191,200,210,236,249,252,254,261],"research":[6],"on":[7,182,233],"development":[9,178],"a":[11,39,73,87,97],"test":[12,104],"system":[13,105],"by":[14,64,220],"polymerase":[15],"chain":[16],"reaction":[17,160],"with":[18,198],"\u00abreal-time\u00bb":[19],"detection":[20],"for":[21,106],"identification":[23],"phytopathogenic":[25],"fungi":[26],"Penicillium":[27,80,107,186,218],"expansum,":[28],"which":[29,58],"cause":[30],"apple":[31],"disease":[32],"\u2013":[33,126,144],"blue":[34],"mold.":[35],"It":[36,85,223],"can":[37],"infect":[38],"wide":[40],"range":[41],"hosts,":[43],"including":[44],"pears,":[45],"strawberries,":[46,195],"tomatoes,":[47],"corn":[48],"and":[49,202,214],"rice.":[50],"P.":[51],"expansum":[52,81,108,219],"produces":[53],"carcinogenic":[55],"metabolite":[56],"patulin,":[57,96],"levels":[59],"in":[60],"food":[61],"are":[62],"regulated":[63],"governments":[66],"many":[68],"countries.":[69],"authors":[71],"selected":[72],"section":[74],"genome,":[76],"gene":[78],"patF":[79],"strain":[82],"NRRL":[83],"35695.":[84],"is":[86,170],"cluster":[88],"genes":[90],"that":[91,226],"mediates":[92],"biosynthesis":[94],"derivative":[98],"tetraquetide":[100],"mycotoxin":[101],"acetate.":[102],"includes":[109],"specific":[110],"primers:":[111],"upstream":[112],"primer":[113,118,158],"(f)":[114],"5\u2019-3\u2019":[115],"GTGGGTCCGCAGGCCTTTAT,":[116],"downstream":[117],"(r)":[119],"3\u2019-5\u2019":[120],"GCCCATTCTCCATCGACCAC.":[121],"Reaction":[122],"setting":[123],"protocol:":[124],"pre-denaturation":[125],"95":[127],"0C-5":[128,133],"minutes":[129],"(1":[130],"cycle);":[131],"denaturation-95":[132],"sec,":[134],"annealing-60":[135],"0C-15":[136],"sec":[137],"(50":[138],"cycles).":[139],"Probe:":[140],"GCCCATTCTCCATCGACCAC,":[141],"fluorescent":[142],"dye":[143],"ROX,":[145],"quencher-BHQ2.":[146],"optimal":[148,165],"concentration":[149,166],"primers":[151],"equal":[152],"to":[153,230,245],"7":[154],"pM":[155],"each":[157],"per":[159],"has":[161],"been":[162],"established.":[163],"probe":[169],"0.4":[171],"pM.":[172],"approbation":[174],"scientific":[177],"was":[179,224,241],"carried":[180,242],"out":[181,243],"29":[183],"strains":[184],"spp.":[187],"isolated":[188],"from":[189,205],"samples":[190,204],"apples,":[192],"eggplants,":[193],"onions,":[194],"grapes,":[196],"peaches":[197],"signs":[199],"spoilage,":[201],"soil":[203],"various":[206],"natural":[207],"geographical":[208],"zones":[209],"Russian":[212,256],"Federation,":[213,257],"presumably":[215],"typed":[216],"as":[217],"dichotomous":[221],"keys.":[222],"established":[225],"only":[227],"15":[228],"belong":[229],"this":[231],"species":[232],"basis":[235],"conducted":[237],"studies.":[238],"study":[240],"according":[244],"thematic":[247],"plan-task":[248],"Ministry":[251],"agriculture":[253],"registration":[259],"number":[260],"INIS":[263],"RTD":[264],"122030200367-8.":[265]},"counts_by_year":[],"updated_date":"2026-11-08T12:12:28.789561","created_date":"2023-08-24T00:00:00"}