{"id":"https://openalex.org/W4385971606","doi":"https://doi.org/10.1021/jacs.3c03991","title":"Genome Mining and Discovery of Imiditides, a Family of RiPPs with a Class-Defining Aspartimide Modification","display_name":"Genome Mining and Discovery of Imiditides, a Family of RiPPs with a Class-Defining Aspartimide Modification","publication_year":2023,"publication_date":"2023-08-18","ids":{"openalex":"https://openalex.org/W4385971606","doi":"https://doi.org/10.1021/jacs.3c03991","pmid":"https://pubmed.ncbi.nlm.nih.gov/37066262","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/10104114"},"language":"en","primary_location":{"id":"doi:10.1021/jacs.3c03991","doi":"https://doi.org/10.1021/jacs.3c03991","is_oa":false,"landing_page_url":"https://doi.org/10.1021/jacs.3c03991","pdf_url":null,"source":{"id":"https://openalex.org/S111155417","display_name":"Journal of the American Chemical Society","issn_l":"0002-7863","issn":["0002-7863","1520-5126","1943-2984"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Chemical Society","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2023/04/07/2023.04.07.536058.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5024923976","display_name":"Li Cao","orcid":"https://orcid.org/0000-0002-0276-6056","observed_orcids":["https://orcid.org/0000-0002-0276-6056"]},"institutions":[{"id":"https://openalex.org/I20089843","display_name":"Princeton University","ror":"https://ror.org/00hx57361","country_code":"US","type":"education","lineage":["https://openalex.org/I20089843"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Li Cao","raw_affiliation_strings":["Department of Chemical and Biological Engineering, Princeton University, Princeton, New Jersey 08544, United States"],"raw_orcid":"https://orcid.org/0000-0002-0276-6056","affiliations":[{"raw_affiliation_string":"Department of Chemical and Biological Engineering, Princeton University, Princeton, New Jersey 08544, United States","institution_ids":["https://openalex.org/I20089843"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5025930575","display_name":"Truc Do","orcid":"https://orcid.org/0000-0001-7212-0296","observed_orcids":["https://orcid.org/0000-0001-7212-0296"]},"institutions":[{"id":"https://openalex.org/I20089843","display_name":"Princeton University","ror":"https://ror.org/00hx57361","country_code":"US","type":"education","lineage":["https://openalex.org/I20089843"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Truc Do","raw_affiliation_strings":["Department of Chemical and Biological Engineering, Princeton University, Princeton, New Jersey 08544, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical and Biological Engineering, Princeton University, Princeton, New Jersey 08544, United States","institution_ids":["https://openalex.org/I20089843"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102917640","display_name":"Angela G. Zhu","orcid":"https://orcid.org/0009-0008-7817-9545","observed_orcids":["https://orcid.org/0009-0008-7817-9545"]},"institutions":[{"id":"https://openalex.org/I20089843","display_name":"Princeton University","ror":"https://ror.org/00hx57361","country_code":"US","type":"education","lineage":["https://openalex.org/I20089843"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Angela Zhu","raw_affiliation_strings":["Department of Chemical and Biological Engineering, Princeton University, Princeton, New Jersey 08544, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical and Biological Engineering, Princeton University, Princeton, New Jersey 08544, United States","institution_ids":["https://openalex.org/I20089843"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113047597","display_name":"Jianshu Duan","orcid":"https://orcid.org/0000-0002-3015-1147","observed_orcids":["https://orcid.org/0000-0002-3015-1147"]},"institutions":[{"id":"https://openalex.org/I20089843","display_name":"Princeton University","ror":"https://ror.org/00hx57361","country_code":"US","type":"education","lineage":["https://openalex.org/I20089843"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jianshu Duan","raw_affiliation_strings":["Department of Geosciences, Princeton University, Princeton, New Jersey 08544, United States"],"raw_orcid":"https://orcid.org/0000-0002-3015-1147","affiliations":[{"raw_affiliation_string":"Department of Geosciences, Princeton University, Princeton, New Jersey 08544, United States","institution_ids":["https://openalex.org/I20089843"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5008795320","display_name":"Nathan Alam","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I20089843","display_name":"Princeton University","ror":"https://ror.org/00hx57361","country_code":"US","type":"education","lineage":["https://openalex.org/I20089843"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Nathan Alam","raw_affiliation_strings":["Department of Chemical and Biological Engineering, Princeton University, Princeton, New Jersey 08544, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical and Biological Engineering, Princeton University, Princeton, New Jersey 08544, United States","institution_ids":["https://openalex.org/I20089843"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5005422184","display_name":"A. James Link","orcid":"https://orcid.org/0000-0001-5066-9691","observed_orcids":["https://orcid.org/0000-0001-5066-9691"]},"institutions":[{"id":"https://openalex.org/I20089843","display_name":"Princeton University","ror":"https://ror.org/00hx57361","country_code":"US","type":"education","lineage":["https://openalex.org/I20089843"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"A. James Link","raw_affiliation_strings":["Department of Chemical and Biological Engineering, Princeton University, Princeton, New Jersey 08544, United States","Department of Chemistry, Princeton University, Princeton, New Jersey 08544, United States","Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, United States"],"raw_orcid":"https://orcid.org/0000-0001-5066-9691","affiliations":[{"raw_affiliation_string":"Department of Chemical and Biological Engineering, Princeton University, Princeton, New Jersey 08544, United States","institution_ids":["https://openalex.org/I20089843"]},{"raw_affiliation_string":"Department of Chemistry, Princeton University, Princeton, New Jersey 08544, United States","institution_ids":["https://openalex.org/I20089843"]},{"raw_affiliation_string":"Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, United States","institution_ids":["https://openalex.org/I20089843"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5005422184"],"corresponding_institution_ids":["https://openalex.org/I20089843"],"apc_list":{"value":5000,"currency":"USD","value_usd":5000},"apc_paid":null,"fwci":1.7674,"has_fulltext":true,"cited_by_count":16,"citation_normalized_percentile":{"value":0.84491878,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"145","issue":"34","first_page":"18834","last_page":"18845"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10252","display_name":"Microbial Natural Products and Biosynthesis","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2402","display_name":"Applied Microbiology and Biotechnology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10252","display_name":"Microbial Natural Products and Biosynthesis","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2402","display_name":"Applied Microbiology and Biotechnology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11103","display_name":"Antimicrobial Peptides and Activities","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/ribosomally-synthesized-and-post-translationally-modified-peptides","display_name":"ribosomally synthesized and post-translationally modified peptides","score":0.9710000157356262},{"id":"https://openalex.org/keywords/o-methyltransferase","display_name":"O-methyltransferase","score":0.9319999814033508},{"id":"https://openalex.org/keywords/biosynthetic-gene-clusters","display_name":"biosynthetic gene clusters","score":0.9070000052452087},{"id":"https://openalex.org/keywords/genome-mining","display_name":"genome mining","score":0.9150000214576721},{"id":"https://openalex.org/keywords/heterologous-expression","display_name":"heterologous expression","score":0.7680000066757202},{"id":"https://openalex.org/keywords/substrate-specificity","display_name":"substrate specificity","score":0.7540000081062317},{"id":"https://openalex.org/keywords/charge-charge-interactions","display_name":"charge-charge interactions","score":0.8389999866485596},{"id":"https://openalex.org/keywords/gram-positive-bacteria","display_name":"Gram-positive bacteria","score":0.7960000038146973},{"id":"https://openalex.org/keywords/natural-product-discovery","display_name":"natural product discovery","score":0.5419999957084656}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.8902002573013306},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.6059762239456177},{"id":"https://openalex.org/C2777212361","wikidata":"https://www.wikidata.org/wiki/Q5127848","display_name":"Class (philosophy)","level":2,"score":0.5571994781494141},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.542044997215271},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.19909211993217468},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.16182392835617065},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.13722291588783264},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.062421709299087524},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.0}],"mesh":[],"locations_count":6,"locations":[{"id":"doi:10.1021/jacs.3c03991","doi":"https://doi.org/10.1021/jacs.3c03991","is_oa":false,"landing_page_url":"https://doi.org/10.1021/jacs.3c03991","pdf_url":null,"source":{"id":"https://openalex.org/S111155417","display_name":"Journal of the American Chemical Society","issn_l":"0002-7863","issn":["0002-7863","1520-5126","1943-2984"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Chemical Society","raw_type":"journal-article"},{"id":"doi:10.1101/2023.04.07.536058","doi":"https://doi.org/10.1101/2023.04.07.536058","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2023.04.07.536058","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2023/04/07/2023.04.07.536058.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},{"id":"pmid:37066262","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/37066262","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"bioRxiv : the preprint server for biology","raw_type":"Preprint"},{"id":"pmid:37595015","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/37595015","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Chemical Society","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:10947588","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/10947588","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC10947588/pdf/nihms-1977028.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"J Am Chem Soc","raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:10104114","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/10104114","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1101/2023.04.07.536058","doi":"https://doi.org/10.1101/2023.04.07.536058","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2023.04.07.536058","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2023/04/07/2023.04.07.536058.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2145591899","display_name":"Collaborative Research: The chemistry of lignin's photochemical transformation in the environment: implications for global carbon cycling","funder_award_id":"1609927","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"},{"id":"https://openalex.org/G3368294025","display_name":"Graduate Research Fellowship Program (GRFP)","funder_award_id":"2039656","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"},{"id":"https://openalex.org/G4256911044","display_name":"Graduate Research Fellowship Program (GRFP)","funder_award_id":"1656466","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"},{"id":"https://openalex.org/G4875142421","display_name":"Lasso Peptide Discovery","funder_award_id":"R01GM107036","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"},{"id":"https://openalex.org/F4320309292","display_name":"Princeton University","ror":"https://ror.org/00hx57361"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4385971606.pdf","grobid_xml":"https://content.openalex.org/works/W4385971606.grobid-xml"},"referenced_works_count":42,"referenced_works":["https://openalex.org/W1534259153","https://openalex.org/W1841821181","https://openalex.org/W1944789189","https://openalex.org/W1965997737","https://openalex.org/W1988202807","https://openalex.org/W2005309431","https://openalex.org/W2006141434","https://openalex.org/W2006502788","https://openalex.org/W2049286919","https://openalex.org/W2051185176","https://openalex.org/W2052038710","https://openalex.org/W2053819820","https://openalex.org/W2082667898","https://openalex.org/W2088539765","https://openalex.org/W2132087321","https://openalex.org/W2142498662","https://openalex.org/W2157009395","https://openalex.org/W2162854463","https://openalex.org/W2326701454","https://openalex.org/W2528897472","https://openalex.org/W2800342017","https://openalex.org/W2899779000","https://openalex.org/W2921257516","https://openalex.org/W2950570200","https://openalex.org/W2961911698","https://openalex.org/W2977984311","https://openalex.org/W2990344250","https://openalex.org/W3015980346","https://openalex.org/W3087348949","https://openalex.org/W3117886555","https://openalex.org/W3122611304","https://openalex.org/W3177828909","https://openalex.org/W3183478041","https://openalex.org/W3212607949","https://openalex.org/W4281790889","https://openalex.org/W4292325769","https://openalex.org/W4293102559","https://openalex.org/W4293788647","https://openalex.org/W4306810360","https://openalex.org/W4307230168","https://openalex.org/W4309184619","https://openalex.org/W4318142409"],"related_works":["https://openalex.org/W4376271492","https://openalex.org/W7134071655","https://openalex.org/W4388869831","https://openalex.org/W4393166875","https://openalex.org/W4406125214","https://openalex.org/W3190075896","https://openalex.org/W3087348949","https://openalex.org/W4309184619","https://openalex.org/W4292325769","https://openalex.org/W3117945984"],"abstract_inverted_index":{"Abstract":[0],"Ribosomally":[1],"synthesized":[2],"and":[3,80,223,266,287],"post-translationally":[4],"modified":[5],"peptides":[6,160],"(RiPPs)":[7],"are":[8,74,280],"a":[9,102,112,172],"fascinating":[10],"class":[11],"of":[12,15,105,114,131,135,146,203],"natural":[13],"products":[14],"ribosomal":[16],"origins.":[17],"In":[18,124,150],"the":[19,39,88,97,128,132,136,144,163,177,201,204,220,224,258],"past":[20],"decade,":[21],"various":[22,51],"sophisticated":[23],"machine":[24],"learning-based":[25],"software":[26],"packages":[27],"have":[28],"been":[29],"established":[30],"to":[31,69,92,100,152,200,257,283],"discover":[32,101],"novel":[33,61,103],"RiPPs":[34],"that":[35,45,48,65,157,243,273],"do":[36],"not":[37,281],"resemble":[38],"known":[40],"families.":[41],"Instead,":[42],"we":[43,86,126],"argue":[44],"tailoring":[46],"enzymes":[47],"cluster":[49],"with":[50,76],"RiPP":[52,62,154,254],"families":[53],"can":[54],"serve":[55],"as":[56,96,161,233,235,264],"effective":[57],"bioinformatic":[58],"seeds":[59],"for":[60],"discovery.":[63],"Leveraging":[64],"O":[66,94],"-methyltransferases":[67,95],"homologous":[68],"protein":[70,285],"isoaspartyl":[71],"methyltransferases":[72],"(PIMTs)":[73],"associated":[75,155],"lasso":[77],"peptide,":[78,180],"graspetide,":[79],"lanthipeptide":[81],"biosynthetic":[82],"gene":[83],"clusters":[84],"(BGCs),":[85],"utilized":[87],"C-terminal":[89],"motif":[90],"unique":[91],"RiPP-associated":[93],"search":[98],"query":[99],"family":[104],"RiPPs,":[106],"imiditides.":[107],"Our":[108,240],"genome-mining":[109],"algorithm":[110],"reveals":[111],"total":[113],"670":[115],"imiditide":[116,137],"BGCs,":[117],"widely":[118],"distributed":[119],"in":[120,143,166,207,253,276],"Gram-positive":[121,277],"bacterial":[122,278],"genomes.":[123],"addition,":[125],"demonstrate":[127],"heterologous":[129],"production":[130],"founding":[133],"member":[134],"family,":[138],"mNmaA":[139,167],"M":[140,168],",":[141],"encoded":[142],"genome":[145],"Nonomuraea":[147],"maritima":[148],".":[149,209],"contrast":[151],"other":[153],"PIMTs":[156],"recognize":[158],"constrained":[159],"substrates,":[162],"PIMT":[164],"homolog":[165],"BGC,":[169],"NmaM,":[170],"methylates":[171],"specific":[173],"Asp":[174],"residue":[175],"on":[176],"linear":[178],"precursor":[179,221],"NmaA.":[181],"The":[182,192,210],"methyl":[183],"ester":[184],"is":[185,196,213,247],"then":[186],"turned":[187],"into":[188],"an":[189,236,248],"aspartimide":[190,193,245,274],"spontaneously.":[191],"moiety":[194],"formed":[195],"unusually":[197],"stable,":[198],"leading":[199],"accumulation":[202],"aspartimidylated":[205],"product":[206],"vivo":[208],"substrate":[211],"specificity":[212],"achieved":[214],"by":[215,229],"extensive":[216],"charge-charge":[217],"interactions":[218],"between":[219],"NmaA":[222],"modifying":[225],"enzyme":[226],"NmaM":[227],"suggested":[228],"both":[230],"experimental":[231],"validations":[232],"well":[234],"AlphaFold":[237],"model":[238],"prediction.":[239],"study":[241],"suggests":[242],"PIMT-mediated":[244],"formation":[246,275],"underappreciated":[249],"backbone":[250,260],"modification":[251],"strategy":[252],"biosynthesis,":[255],"compared":[256],"well-studied":[259],"rigidification":[261],"chemistries,":[262],"such":[263],"thiazol(in)e":[265],"oxazol(in)e":[267],"formations.":[268],"Additionally,":[269],"our":[270],"findings":[271],"suggest":[272],"proteomes":[279],"limited":[282],"spontaneous":[284],"aging":[286],"degradation.":[288],"TOC":[289],"Figure":[290]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":8},{"year":2024,"cited_by_count":5},{"year":2023,"cited_by_count":1}],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2023-08-19T00:00:00"}