{"id":"https://openalex.org/W4385803472","doi":"https://doi.org/10.7554/elife.87306.2","title":"Self-formation of concentric zones of telencephalic and ocular tissues and directional retinal ganglion cell axons","display_name":"Self-formation of concentric zones of telencephalic and ocular tissues and directional retinal ganglion cell axons","publication_year":2023,"publication_date":"2023-08-14","ids":{"openalex":"https://openalex.org/W4385803472","doi":"https://doi.org/10.7554/elife.87306.2"},"language":"en","primary_location":{"id":"doi:10.7554/elife.87306.2","doi":"https://doi.org/10.7554/elife.87306.2","is_oa":true,"landing_page_url":"https://doi.org/10.7554/elife.87306.2","pdf_url":null,"source":{"id":"https://openalex.org/S1336409049","display_name":"eLife","issn_l":"2050-084X","issn":["2050-084X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","jufo-2","ki-jl-2","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311710","host_organization_name":"eLife Sciences Publications Ltd","host_organization_lineage":["https://openalex.org/P4310311710"],"host_organization_lineage_names":["eLife Sciences Publications Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"eLife","raw_type":"posted-content"},"volume":null,"type":"preprint","indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.7554/elife.87306.2","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100431996","display_name":"Wei Liu","orcid":"https://orcid.org/0000-0003-4199-5406","observed_orcids":["https://orcid.org/0000-0003-4199-5406"]},"institutions":[{"id":"https://openalex.org/I4210087873","display_name":"Institute for Stem Cell Biology and Regenerative Medicine","ror":"https://ror.org/007wpch15","country_code":"IN","type":"facility","lineage":["https://openalex.org/I2799351866","https://openalex.org/I3141573830","https://openalex.org/I4210087873","https://openalex.org/I4210134808"]},{"id":"https://openalex.org/I4394709089","display_name":"Institute for Stem Cell Biology and Regenerative Medicine","ror":"https://ror.org/02vbab064","country_code":"US","type":"facility","lineage":["https://openalex.org/I4210137306","https://openalex.org/I4394709089","https://openalex.org/I97018004"]}],"countries":["IN","US"],"is_corresponding":false,"raw_author_name":"Wei Liu","raw_affiliation_strings":["Department of Genetics","Department of Ophthalmology and Visual Sciences","The Ruth L. and David S. Gottesman Institute for Stem Cell Biology and Regenerative Medicine","Department of Genetics; Department of Ophthalmology and Visual Sciences; The Ruth L. and David S. Gottesman Institute for Stem Cell Biology and Regenerative Medicine"],"raw_orcid":"https://orcid.org/0000-0003-4199-5406","affiliations":[{"raw_affiliation_string":"Department of Genetics","institution_ids":[]},{"raw_affiliation_string":"Department of Ophthalmology and Visual Sciences","institution_ids":[]},{"raw_affiliation_string":"The Ruth L. and David S. Gottesman Institute for Stem Cell Biology and Regenerative Medicine","institution_ids":["https://openalex.org/I4210087873"]},{"raw_affiliation_string":"Department of Genetics; Department of Ophthalmology and Visual Sciences; The Ruth L. and David S. Gottesman Institute for Stem Cell Biology and Regenerative Medicine","institution_ids":["https://openalex.org/I4210087873","https://openalex.org/I4394709089"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103273611","display_name":"Rupendra Shrestha","orcid":"https://orcid.org/0000-0001-6804-6070","observed_orcids":["https://orcid.org/0000-0001-6804-6070"]},"institutions":[{"id":"https://openalex.org/I4210087873","display_name":"Institute for Stem Cell Biology and Regenerative Medicine","ror":"https://ror.org/007wpch15","country_code":"IN","type":"facility","lineage":["https://openalex.org/I2799351866","https://openalex.org/I3141573830","https://openalex.org/I4210087873","https://openalex.org/I4210134808"]},{"id":"https://openalex.org/I4394709089","display_name":"Institute for Stem Cell Biology and Regenerative Medicine","ror":"https://ror.org/02vbab064","country_code":"US","type":"facility","lineage":["https://openalex.org/I4210137306","https://openalex.org/I4394709089","https://openalex.org/I97018004"]}],"countries":["IN","US"],"is_corresponding":false,"raw_author_name":"Rupendra Shrestha","raw_affiliation_strings":["Department of Genetics","Department of Ophthalmology and Visual Sciences","The Ruth L. and David S. Gottesman Institute for Stem Cell Biology and Regenerative Medicine","Department of Genetics; Department of Ophthalmology and Visual Sciences; The Ruth L. and David S. Gottesman Institute for Stem Cell Biology and Regenerative Medicine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Genetics","institution_ids":[]},{"raw_affiliation_string":"Department of Ophthalmology and Visual Sciences","institution_ids":[]},{"raw_affiliation_string":"The Ruth L. and David S. Gottesman Institute for Stem Cell Biology and Regenerative Medicine","institution_ids":["https://openalex.org/I4210087873"]},{"raw_affiliation_string":"Department of Genetics; Department of Ophthalmology and Visual Sciences; The Ruth L. and David S. Gottesman Institute for Stem Cell Biology and Regenerative Medicine","institution_ids":["https://openalex.org/I4210087873","https://openalex.org/I4394709089"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5067839678","display_name":"Albert Lowe","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Albert Lowe","raw_affiliation_strings":["Department of Genetics","Department of Ophthalmology and Visual Sciences","Department of Genetics; Department of Ophthalmology and Visual Sciences"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Genetics","institution_ids":[]},{"raw_affiliation_string":"Department of Ophthalmology and Visual Sciences","institution_ids":[]},{"raw_affiliation_string":"Department of Genetics; Department of Ophthalmology and Visual Sciences","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5007696021","display_name":"Xusheng Zhang","orcid":"https://orcid.org/0000-0002-9335-5577","observed_orcids":["https://orcid.org/0000-0002-9335-5577"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Xusheng Zhang","raw_affiliation_strings":["Department of Genetics"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Genetics","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5073205297","display_name":"Ludovic Spaeth","orcid":"https://orcid.org/0000-0001-9161-1365","observed_orcids":["https://orcid.org/0000-0001-9161-1365"]},"institutions":[{"id":"https://openalex.org/I129975664","display_name":"Albert Einstein College of Medicine","ror":"https://ror.org/05cf8a891","country_code":"US","type":"education","lineage":["https://openalex.org/I129975664","https://openalex.org/I4210112371"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ludovic Spaeth","raw_affiliation_strings":["Dominick P. Purpura Department of Neuroscience Albert Einstein College of Medicine","Dominick P. Purpura Department of Neuroscience Albert Einstein College of Medicine, Bronx, NY 10461"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dominick P. Purpura Department of Neuroscience Albert Einstein College of Medicine","institution_ids":["https://openalex.org/I129975664"]},{"raw_affiliation_string":"Dominick P. Purpura Department of Neuroscience Albert Einstein College of Medicine, Bronx, NY 10461","institution_ids":["https://openalex.org/I129975664"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3000,"currency":"USD","value_usd":2500},"apc_paid":{"value":3000,"currency":"USD","value_usd":2500},"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10691","display_name":"Retinal Development and Disorders","score":0.8070999979972839,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10691","display_name":"Retinal Development and Disorders","score":0.8070999979972839,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10608","display_name":"Neurogenesis and neuroplasticity mechanisms","score":0.07509999722242355,"subfield":{"id":"https://openalex.org/subfields/2806","display_name":"Developmental Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12061","display_name":"Axon Guidance and Neuronal Signaling","score":0.046799998730421066,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/optic-disc","display_name":"optic nerve head","score":0.6299999952316284},{"id":"https://openalex.org/keywords/optic-stalk","display_name":"optic stalk","score":0.9610000252723694},{"id":"https://openalex.org/keywords/neuroretina","display_name":"neuroretina","score":0.824999988079071},{"id":"https://openalex.org/keywords/anterior-neural-plate","display_name":"anterior neural plate","score":0.7139999866485596},{"id":"https://openalex.org/keywords/fgf8","display_name":"FGF8","score":0.7599999904632568},{"id":"https://openalex.org/keywords/fgf9","display_name":"FGF9","score":0.9959999918937683},{"id":"https://openalex.org/keywords/fgfr-inhibition","display_name":"FGFR inhibition","score":0.878000020980835},{"id":"https://openalex.org/keywords/contactin-2","display_name":"CNTN2","score":0.9869999885559082},{"id":"https://openalex.org/keywords/single-cell-rna-sequencing","display_name":"single-cell RNA sequencing","score":0.6980000138282776},{"id":"https://openalex.org/keywords/glaucoma","display_name":"glaucoma","score":0.6629999876022339}],"concepts":[{"id":"https://openalex.org/C533869091","wikidata":"https://www.wikidata.org/wiki/Q75855","display_name":"Cerebrum","level":3,"score":0.7750514149665833},{"id":"https://openalex.org/C2777624874","wikidata":"https://www.wikidata.org/wiki/Q927337","display_name":"Retinal ganglion cell","level":3,"score":0.7257293462753296},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7255586385726929},{"id":"https://openalex.org/C2780837183","wikidata":"https://www.wikidata.org/wiki/Q186029","display_name":"Optic nerve","level":2,"score":0.6490496397018433},{"id":"https://openalex.org/C169760540","wikidata":"https://www.wikidata.org/wiki/Q207011","display_name":"Neuroscience","level":1,"score":0.5519329309463501},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.5469635725021362},{"id":"https://openalex.org/C2777093970","wikidata":"https://www.wikidata.org/wiki/Q169342","display_name":"Retina","level":2,"score":0.5460436940193176},{"id":"https://openalex.org/C2779530196","wikidata":"https://www.wikidata.org/wiki/Q178999","display_name":"Axon","level":2,"score":0.5436418056488037},{"id":"https://openalex.org/C69125201","wikidata":"https://www.wikidata.org/wiki/Q2034716","display_name":"Axon guidance","level":3,"score":0.4142317771911621},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3298734724521637},{"id":"https://openalex.org/C529278444","wikidata":"https://www.wikidata.org/wiki/Q47273","display_name":"Central nervous system","level":2,"score":0.20364797115325928}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.7554/elife.87306.2","doi":"https://doi.org/10.7554/elife.87306.2","is_oa":true,"landing_page_url":"https://doi.org/10.7554/elife.87306.2","pdf_url":null,"source":{"id":"https://openalex.org/S1336409049","display_name":"eLife","issn_l":"2050-084X","issn":["2050-084X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","jufo-2","ki-jl-2","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311710","host_organization_name":"eLife Sciences Publications Ltd","host_organization_lineage":["https://openalex.org/P4310311710"],"host_organization_lineage_names":["eLife Sciences Publications Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"eLife","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.7554/elife.87306.2","doi":"https://doi.org/10.7554/elife.87306.2","is_oa":true,"landing_page_url":"https://doi.org/10.7554/elife.87306.2","pdf_url":null,"source":{"id":"https://openalex.org/S1336409049","display_name":"eLife","issn_l":"2050-084X","issn":["2050-084X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","jufo-2","ki-jl-2","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311710","host_organization_name":"eLife Sciences Publications Ltd","host_organization_lineage":["https://openalex.org/P4310311710"],"host_organization_lineage_names":["eLife Sciences Publications Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"eLife","raw_type":"posted-content"},"sustainable_development_goals":[{"score":0.6240000128746033,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G4245999059","display_name":"Modeling CRB1-related retinal disease using 3-D human retinal organoids","funder_award_id":"R21EY029806","funder_id":"https://openalex.org/F4320337350","funder_display_name":"National Eye Institute"},{"id":"https://openalex.org/G6872674026","display_name":"Cerebellar Dysfunction in DYT1","funder_award_id":"R01NS105470","funder_id":"https://openalex.org/F4320337359","funder_display_name":"National Institute of Neurological Disorders and Stroke"},{"id":"https://openalex.org/G7764720279","display_name":"Training Program in Cellular and Molecular Biology and Genetics","funder_award_id":"T32GM007491","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G9000918654","display_name":"Gene regulation of retinal cell differentiation","funder_award_id":"R01EY022645","funder_id":"https://openalex.org/F4320337350","funder_display_name":"National Eye Institute"}],"funders":[{"id":"https://openalex.org/F4320337350","display_name":"National Eye Institute","ror":"https://ror.org/03wkg3b53"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"},{"id":"https://openalex.org/F4320337359","display_name":"National Institute of Neurological Disorders and Stroke","ror":"https://ror.org/01s5ya894"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":85,"referenced_works":["https://openalex.org/W1968575236","https://openalex.org/W1970783808","https://openalex.org/W1973029281","https://openalex.org/W1974644062","https://openalex.org/W1976343564","https://openalex.org/W1976754131","https://openalex.org/W1985901090","https://openalex.org/W1988017462","https://openalex.org/W1988939522","https://openalex.org/W1989920563","https://openalex.org/W1993606572","https://openalex.org/W1993686667","https://openalex.org/W1994009311","https://openalex.org/W2004550343","https://openalex.org/W2005943162","https://openalex.org/W2006196740","https://openalex.org/W2007081540","https://openalex.org/W2018286352","https://openalex.org/W2020386821","https://openalex.org/W2021186319","https://openalex.org/W2024215958","https://openalex.org/W2024846396","https://openalex.org/W2029279488","https://openalex.org/W2029559054","https://openalex.org/W2030535403","https://openalex.org/W2031202161","https://openalex.org/W2035797804","https://openalex.org/W2036505824","https://openalex.org/W2037723919","https://openalex.org/W2044120077","https://openalex.org/W2044928246","https://openalex.org/W2051785391","https://openalex.org/W2052867854","https://openalex.org/W2063288238","https://openalex.org/W2066867136","https://openalex.org/W2068099401","https://openalex.org/W2073947789","https://openalex.org/W2074314696","https://openalex.org/W2075626782","https://openalex.org/W2078643425","https://openalex.org/W2080937698","https://openalex.org/W2081407876","https://openalex.org/W2087786585","https://openalex.org/W2102428207","https://openalex.org/W2102607379","https://openalex.org/W2106461017","https://openalex.org/W2112678012","https://openalex.org/W2114240336","https://openalex.org/W2126646136","https://openalex.org/W2131060923","https://openalex.org/W2133875152","https://openalex.org/W2134321528","https://openalex.org/W2134374915","https://openalex.org/W2138643777","https://openalex.org/W2142299384","https://openalex.org/W2143210568","https://openalex.org/W2150520945","https://openalex.org/W2151445759","https://openalex.org/W2160142900","https://openalex.org/W2167546055","https://openalex.org/W2170354499","https://openalex.org/W2293433220","https://openalex.org/W2332292689","https://openalex.org/W2343783580","https://openalex.org/W2527991441","https://openalex.org/W2535856472","https://openalex.org/W2541088566","https://openalex.org/W2602914633","https://openalex.org/W2761739377","https://openalex.org/W2789621577","https://openalex.org/W2804583160","https://openalex.org/W2893194701","https://openalex.org/W2944813712","https://openalex.org/W2949020598","https://openalex.org/W2949177718","https://openalex.org/W2992957227","https://openalex.org/W3021129313","https://openalex.org/W3042896257","https://openalex.org/W3087102910","https://openalex.org/W3097920090","https://openalex.org/W3105545386","https://openalex.org/W3171218066","https://openalex.org/W3196117556","https://openalex.org/W4229003364","https://openalex.org/W4281290796"],"related_works":["https://openalex.org/W6902736665","https://openalex.org/W4376875670","https://openalex.org/W7049250999","https://openalex.org/W1970977410","https://openalex.org/W2784113504","https://openalex.org/W2047635351","https://openalex.org/W4414081690","https://openalex.org/W4385968506","https://openalex.org/W2893194701","https://openalex.org/W4392932868"],"abstract_inverted_index":{"Summary":[0],"The":[1],"telencephalon":[2],"and":[3,25,34,67,79,100,112,132,140,170,175],"eye":[4],"in":[5,120,126,173],"mammals":[6],"are":[7,37],"originated":[8],"from":[9],"adjacent":[10,86],"fields":[11,20],"at":[12],"the":[13,54,71,145,164],"anterior":[14],"neural":[15],"plate.":[16],"Morphogenesis":[17],"of":[18,56,63,93,108,167],"these":[19,32],"generates":[21],"telencephalon,":[22],"optic-stalk,":[23,65,111],"optic-disc,":[24,66,110],"neuroretina":[26],"along":[27,70,81],"a":[28,82,156],"spatial":[29],"axis.":[30,73],"How":[31],"telencephalic":[33,99,169],"ocular":[35,101,171],"tissues":[36,69,172],"specified":[38],"coordinately":[39],"to":[40,118],"ensure":[41],"directional":[42,141],"retinal":[43],"ganglion":[44],"cell":[45],"(RGC)":[46],"axon":[47,142],"growth":[48],"is":[49],"unclear.":[50],"Here,":[51],"we":[52],"report":[53],"self-formation":[55],"human":[57,121],"telencephalon-eye":[58],"organoids":[59,95,128],"comprising":[60],"concentric":[61],"zones":[62],"telencephalic,":[64],"neuroretinal":[68],"center-periphery":[72],"Initially-differentiated":[74],"RGCs":[75,152],"grew":[76],"axons":[77],"towards":[78],"then":[80],"path":[83],"defined":[84],"by":[85],"PAX2+":[87],"optic-disc":[88],"cells.":[89],"Single-cell":[90],"RNA":[91],"sequencing":[92],"CONCEPT":[94,127],"not":[96],"only":[97],"confirmed":[98],"identities":[102],"but":[103],"also":[104],"identified":[105,146],"expression":[106],"signatures":[107,115],"early":[109,168],"RGCs.":[113],"These":[114],"were":[116,153],"similar":[117],"those":[119],"fetal":[122],"retinas.":[123],"Optic-disc":[124],"cells":[125],"differentially":[129],"expressed":[130],"FGF8":[131],"FGF9;":[133],"FGFR":[134],"inhibitions":[135],"drastically":[136],"decreased":[137],"RGC":[138],"differentiation":[139],"growth.":[143],"Through":[144],"RGC-specific":[147],"cell-surface":[148],"marker":[149],"CNTN2,":[150],"electrophysiologically-excitable":[151],"isolated":[154],"under":[155],"native":[157],"condition.":[158],"Our":[159],"findings":[160],"provide":[161],"insight":[162],"into":[163],"coordinated":[165],"specification":[166],"humans":[174],"establish":[176],"resources":[177],"for":[178],"studying":[179],"RGC-related":[180],"diseases":[181],"such":[182],"as":[183],"glaucoma.":[184]},"counts_by_year":[],"updated_date":"2026-10-28T10:33:24.094632","created_date":"2023-08-15T00:00:00"}