{"id":"https://openalex.org/W4385758809","doi":"https://doi.org/10.3389/fcimb.2023.1165432","title":"High specificity of metagenomic next-generation sequencing using protected bronchial brushing sample in diagnosing pneumonia in children","display_name":"High specificity of metagenomic next-generation sequencing using protected bronchial brushing sample in diagnosing pneumonia in children","publication_year":2023,"publication_date":"2023-08-11","ids":{"openalex":"https://openalex.org/W4385758809","doi":"https://doi.org/10.3389/fcimb.2023.1165432","pmid":"https://pubmed.ncbi.nlm.nih.gov/37637461","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/10457156"},"language":"en","primary_location":{"id":"doi:10.3389/fcimb.2023.1165432","doi":"https://doi.org/10.3389/fcimb.2023.1165432","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fcimb.2023.1165432","pdf_url":"https://www.frontiersin.org/articles/10.3389/fcimb.2023.1165432/pdf","source":{"id":"https://openalex.org/S2506667490","display_name":"Frontiers in Cellular and Infection Microbiology","issn_l":"2235-2988","issn":["2235-2988"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Cellular and Infection Microbiology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.frontiersin.org/articles/10.3389/fcimb.2023.1165432/pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100350844","display_name":"Chunyan Zhang","orcid":"https://orcid.org/0000-0003-1729-8985","observed_orcids":["https://orcid.org/0000-0003-1729-8985"]},"institutions":[{"id":"https://openalex.org/I154099455","display_name":"Shandong University","ror":"https://ror.org/0207yh398","country_code":"CN","type":"education","lineage":["https://openalex.org/I154099455"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Chunyan Zhang","raw_affiliation_strings":["Department of Clinical Microbiology, Shandong Provincial Clinical Research Center for Children's Health and Disease, Jinan, China","Department of Microbiology Laboratory, Children's Hospital Affiliated to Shandong University, Jinan, China","Department of Clinical Microbiology, Shandong Provincial Clinical Research Center for Children's Health and Disease, China","Department of Microbiology Laboratory, Children's Hospital Affiliated to Shandong University, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Clinical Microbiology, Shandong Provincial Clinical Research Center for Children's Health and Disease, Jinan, China","institution_ids":[]},{"raw_affiliation_string":"Department of Microbiology Laboratory, Children's Hospital Affiliated to Shandong University, Jinan, China","institution_ids":["https://openalex.org/I154099455"]},{"raw_affiliation_string":"Department of Clinical Microbiology, Shandong Provincial Clinical Research Center for Children's Health and Disease, China","institution_ids":[]},{"raw_affiliation_string":"Department of Microbiology Laboratory, Children's Hospital Affiliated to Shandong University, China","institution_ids":["https://openalex.org/I154099455"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100423655","display_name":"Li Zheng","orcid":"https://orcid.org/0000-0001-7313-573X","observed_orcids":["https://orcid.org/0000-0001-7313-573X"]},"institutions":[{"id":"https://openalex.org/I154099455","display_name":"Shandong University","ror":"https://ror.org/0207yh398","country_code":"CN","type":"education","lineage":["https://openalex.org/I154099455"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Zheng Li","raw_affiliation_strings":["Department of Clinical Microbiology, Shandong Provincial Clinical Research Center for Children's Health and Disease, Jinan, China","Department of Microbiology Laboratory, Children's Hospital Affiliated to Shandong University, Jinan, China","Department of Clinical Microbiology, Shandong Provincial Clinical Research Center for Children's Health and Disease, China","Department of Microbiology Laboratory, Children's Hospital Affiliated to Shandong University, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Clinical Microbiology, Shandong Provincial Clinical Research Center for Children's Health and Disease, Jinan, China","institution_ids":[]},{"raw_affiliation_string":"Department of Microbiology Laboratory, Children's Hospital Affiliated to Shandong University, Jinan, China","institution_ids":["https://openalex.org/I154099455"]},{"raw_affiliation_string":"Department of Clinical Microbiology, Shandong Provincial Clinical Research Center for Children's Health and Disease, China","institution_ids":[]},{"raw_affiliation_string":"Department of Microbiology Laboratory, Children's Hospital Affiliated to Shandong University, China","institution_ids":["https://openalex.org/I154099455"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101675518","display_name":"Mengyuan Wang","orcid":"https://orcid.org/0000-0003-2077-361X","observed_orcids":["https://orcid.org/0000-0003-2077-361X"]},"institutions":[{"id":"https://openalex.org/I154099455","display_name":"Shandong University","ror":"https://ror.org/0207yh398","country_code":"CN","type":"education","lineage":["https://openalex.org/I154099455"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Mengyuan Wang","raw_affiliation_strings":["Department of Clinical Microbiology, Shandong Provincial Clinical Research Center for Children's Health and Disease, Jinan, China","Department of Microbiology Laboratory, Children's Hospital Affiliated to Shandong University, Jinan, China","Department of Clinical Microbiology, Shandong Provincial Clinical Research Center for Children's Health and Disease, China","Department of Microbiology Laboratory, Children's Hospital Affiliated to Shandong University, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Clinical Microbiology, Shandong Provincial Clinical Research Center for Children's Health and Disease, Jinan, China","institution_ids":[]},{"raw_affiliation_string":"Department of Microbiology Laboratory, Children's Hospital Affiliated to Shandong University, Jinan, China","institution_ids":["https://openalex.org/I154099455"]},{"raw_affiliation_string":"Department of Clinical Microbiology, Shandong Provincial Clinical Research Center for Children's Health and Disease, China","institution_ids":[]},{"raw_affiliation_string":"Department of Microbiology Laboratory, Children's Hospital Affiliated to Shandong University, China","institution_ids":["https://openalex.org/I154099455"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5065292892","display_name":"Jiemin Zhou","orcid":"https://orcid.org/0000-0001-6317-5973","observed_orcids":["https://orcid.org/0000-0001-6317-5973"]},"institutions":[{"id":"https://openalex.org/I4210110199","display_name":"Vision Medicals (China)","ror":"https://ror.org/01z86wn38","country_code":"CN","type":"company","lineage":["https://openalex.org/I4210110199"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Jiemin Zhou","raw_affiliation_strings":["Department of Scientific Affairs, Vision Medicals Center for Infectious Diseases, Guangzhou, China","Department of Scientific Affairs, Vision Medicals Center for Infectious Diseases, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Scientific Affairs, Vision Medicals Center for Infectious Diseases, Guangzhou, China","institution_ids":["https://openalex.org/I4210110199"]},{"raw_affiliation_string":"Department of Scientific Affairs, Vision Medicals Center for Infectious Diseases, China","institution_ids":["https://openalex.org/I4210110199"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068348950","display_name":"Wenwen Yu","orcid":"https://orcid.org/0000-0003-3613-9111","observed_orcids":["https://orcid.org/0000-0003-3613-9111"]},"institutions":[{"id":"https://openalex.org/I154099455","display_name":"Shandong University","ror":"https://ror.org/0207yh398","country_code":"CN","type":"education","lineage":["https://openalex.org/I154099455"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Wenwen Yu","raw_affiliation_strings":["Department of Clinical Microbiology, Shandong Provincial Clinical Research Center for Children's Health and Disease, Jinan, China","Department of Microbiology Laboratory, Children's Hospital Affiliated to Shandong University, Jinan, China","Department of Clinical Microbiology, Shandong Provincial Clinical Research Center for Children's Health and Disease, China","Department of Microbiology Laboratory, Children's Hospital Affiliated to Shandong University, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Clinical Microbiology, Shandong Provincial Clinical Research Center for Children's Health and Disease, Jinan, China","institution_ids":[]},{"raw_affiliation_string":"Department of Microbiology Laboratory, Children's Hospital Affiliated to Shandong University, Jinan, China","institution_ids":["https://openalex.org/I154099455"]},{"raw_affiliation_string":"Department of Clinical Microbiology, Shandong Provincial Clinical Research Center for Children's Health and Disease, China","institution_ids":[]},{"raw_affiliation_string":"Department of Microbiology Laboratory, Children's Hospital Affiliated to Shandong University, China","institution_ids":["https://openalex.org/I154099455"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100745156","display_name":"Huifang Liu","orcid":"https://orcid.org/0000-0003-1545-5828","observed_orcids":["https://orcid.org/0000-0003-1545-5828"]},"institutions":[{"id":"https://openalex.org/I4210110199","display_name":"Vision Medicals (China)","ror":"https://ror.org/01z86wn38","country_code":"CN","type":"company","lineage":["https://openalex.org/I4210110199"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Huifang Liu","raw_affiliation_strings":["Department of Scientific Affairs, Vision Medicals Center for Infectious Diseases, Guangzhou, China","Department of Scientific Affairs, Vision Medicals Center for Infectious Diseases, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Scientific Affairs, Vision Medicals Center for Infectious Diseases, Guangzhou, China","institution_ids":["https://openalex.org/I4210110199"]},{"raw_affiliation_string":"Department of Scientific Affairs, Vision Medicals Center for Infectious Diseases, China","institution_ids":["https://openalex.org/I4210110199"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109164231","display_name":"Bingxue Hu","orcid":"https://orcid.org/0000-0001-9116-6249","observed_orcids":["https://orcid.org/0000-0001-9116-6249"]},"institutions":[{"id":"https://openalex.org/I4210110199","display_name":"Vision Medicals (China)","ror":"https://ror.org/01z86wn38","country_code":"CN","type":"company","lineage":["https://openalex.org/I4210110199"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Bingxue Hu","raw_affiliation_strings":["Department of Scientific Affairs, Vision Medicals Center for Infectious Diseases, Guangzhou, China","Department of Scientific Affairs, Vision Medicals Center for Infectious Diseases, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Scientific Affairs, Vision Medicals Center for Infectious Diseases, Guangzhou, China","institution_ids":["https://openalex.org/I4210110199"]},{"raw_affiliation_string":"Department of Scientific Affairs, Vision Medicals Center for Infectious Diseases, China","institution_ids":["https://openalex.org/I4210110199"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5027714793","display_name":"Shifu Wang","orcid":"https://orcid.org/0000-0002-2131-9518","observed_orcids":["https://orcid.org/0000-0002-2131-9518"]},"institutions":[{"id":"https://openalex.org/I154099455","display_name":"Shandong University","ror":"https://ror.org/0207yh398","country_code":"CN","type":"education","lineage":["https://openalex.org/I154099455"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Shifu Wang","raw_affiliation_strings":["Department of Clinical Microbiology, Shandong Provincial Clinical Research Center for Children's Health and Disease, Jinan, China","Department of Microbiology Laboratory, Children's Hospital Affiliated to Shandong University, Jinan, China","Department of Clinical Microbiology, Shandong Provincial Clinical Research Center for Children's Health and Disease, China","Department of Microbiology Laboratory, Children's Hospital Affiliated to Shandong University, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Clinical Microbiology, Shandong Provincial Clinical Research Center for Children's Health and Disease, Jinan, China","institution_ids":[]},{"raw_affiliation_string":"Department of Microbiology Laboratory, Children's Hospital Affiliated to Shandong University, Jinan, China","institution_ids":["https://openalex.org/I154099455"]},{"raw_affiliation_string":"Department of Clinical Microbiology, Shandong Provincial Clinical Research Center for Children's Health and Disease, China","institution_ids":[]},{"raw_affiliation_string":"Department of Microbiology Laboratory, Children's Hospital Affiliated to Shandong University, China","institution_ids":["https://openalex.org/I154099455"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5027714793"],"corresponding_institution_ids":["https://openalex.org/I154099455"],"apc_list":{"value":3295,"currency":"USD","value_usd":3295},"apc_paid":{"value":3295,"currency":"USD","value_usd":3295},"fwci":1.6287,"has_fulltext":true,"cited_by_count":9,"citation_normalized_percentile":{"value":0.81721971,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":91,"max":98},"biblio":{"volume":"13","issue":null,"first_page":"1165432","last_page":"1165432"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10654","display_name":"Pneumonia and Respiratory Infections","score":0.5512999892234802,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10654","display_name":"Pneumonia and Respiratory Infections","score":0.5512999892234802,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12167","display_name":"Bacterial Identification and Susceptibility Testing","score":0.3659999966621399,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11688","display_name":"Microbial infections and disease research","score":0.05310000106692314,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/metagenomic-next-generation-sequencing","display_name":"metagenomic next-generation sequencing","score":0.9399999976158142},{"id":"https://openalex.org/keywords/bronchoalveolar-lavage-fluid","display_name":"bronchoalveolar lavage fluid","score":0.9900000095367432},{"id":"https://openalex.org/keywords/childhood-pneumonia","display_name":"pediatric pneumonia","score":0.9200000166893005},{"id":"https://openalex.org/keywords/lower-respiratory-tract-infection","display_name":"lower respiratory tract infection","score":0.7260000109672546},{"id":"https://openalex.org/keywords/pathogen-detection","display_name":"pathogen detection","score":0.8539999723434448},{"id":"https://openalex.org/keywords/diagnostic-specificity","display_name":"diagnostic specificity","score":0.9729999899864197},{"id":"https://openalex.org/keywords/mycoplasma-pneumoniae","display_name":"Mycoplasma pneumoniae","score":0.9810000061988831}],"concepts":[{"id":"https://openalex.org/C2779215961","wikidata":"https://www.wikidata.org/wiki/Q310904","display_name":"Mycoplasma pneumoniae","level":3,"score":0.7491318583488464},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.7270914316177368},{"id":"https://openalex.org/C2777914695","wikidata":"https://www.wikidata.org/wiki/Q12192","display_name":"Pneumonia","level":2,"score":0.6433148384094238},{"id":"https://openalex.org/C2777961210","wikidata":"https://www.wikidata.org/wiki/Q927148","display_name":"Bronchoalveolar lavage","level":3,"score":0.615169107913971},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.5685514807701111},{"id":"https://openalex.org/C90924648","wikidata":"https://www.wikidata.org/wiki/Q120569","display_name":"Gastroenterology","level":1,"score":0.48874735832214355},{"id":"https://openalex.org/C15151743","wikidata":"https://www.wikidata.org/wiki/Q903778","display_name":"Metagenomics","level":3,"score":0.4538775682449341},{"id":"https://openalex.org/C2779644171","wikidata":"https://www.wikidata.org/wiki/Q13400765","display_name":"Respiratory tract","level":3,"score":0.42599251866340637},{"id":"https://openalex.org/C534529494","wikidata":"https://www.wikidata.org/wiki/Q7891","display_name":"Respiratory system","level":2,"score":0.3859248459339142},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.19262120127677917},{"id":"https://openalex.org/C2777714996","wikidata":"https://www.wikidata.org/wiki/Q7886","display_name":"Lung","level":2,"score":0.15255427360534668},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D002648","descriptor_name":"Child","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009177","descriptor_name":"Mycoplasma pneumoniae","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011014","descriptor_name":"Pneumonia","qualifier_ui":"Q000175","qualifier_name":"diagnosis","is_major_topic":true},{"descriptor_ui":"D012141","descriptor_name":"Respiratory Tract Infections","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012189","descriptor_name":"Retrospective Studies","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D056186","descriptor_name":"Metagenomics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D059014","descriptor_name":"High-Throughput Nucleotide Sequencing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.3389/fcimb.2023.1165432","doi":"https://doi.org/10.3389/fcimb.2023.1165432","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fcimb.2023.1165432","pdf_url":"https://www.frontiersin.org/articles/10.3389/fcimb.2023.1165432/pdf","source":{"id":"https://openalex.org/S2506667490","display_name":"Frontiers in Cellular and Infection Microbiology","issn_l":"2235-2988","issn":["2235-2988"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Cellular and Infection Microbiology","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:10457156","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/10457156","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC10457156/pdf/fcimb-13-1165432.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Front Cell Infect Microbiol","raw_type":"Text"},{"id":"pmid:37637461","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/37637461","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in cellular and infection microbiology","raw_type":"Journal Article"},{"id":"pmh:oai:doaj.org/article:29e3d851f9e14c0990a0a5163d0c3004","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/29e3d851f9e14c0990a0a5163d0c3004","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Frontiers in Cellular and Infection Microbiology, Vol 13 (2023)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.3389/fcimb.2023.1165432","doi":"https://doi.org/10.3389/fcimb.2023.1165432","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fcimb.2023.1165432","pdf_url":"https://www.frontiersin.org/articles/10.3389/fcimb.2023.1165432/pdf","source":{"id":"https://openalex.org/S2506667490","display_name":"Frontiers in Cellular and Infection Microbiology","issn_l":"2235-2988","issn":["2235-2988"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Cellular and Infection Microbiology","raw_type":"journal-article"},"sustainable_development_goals":[{"score":1.0,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[{"display_name":"Observational Study","id":"https://openalex.org/study-designs/observational-study"}],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4385758809.pdf","grobid_xml":"https://content.openalex.org/works/W4385758809.grobid-xml"},"referenced_works_count":46,"referenced_works":["https://openalex.org/W1575675995","https://openalex.org/W1900247611","https://openalex.org/W1906611030","https://openalex.org/W1972249440","https://openalex.org/W1972952027","https://openalex.org/W1986853053","https://openalex.org/W1998972425","https://openalex.org/W2000714505","https://openalex.org/W2008880662","https://openalex.org/W2103441770","https://openalex.org/W2106780559","https://openalex.org/W2159153735","https://openalex.org/W2258831804","https://openalex.org/W2473016454","https://openalex.org/W2619324389","https://openalex.org/W2621720685","https://openalex.org/W2805442283","https://openalex.org/W2902231090","https://openalex.org/W2913066251","https://openalex.org/W2944437597","https://openalex.org/W2981903737","https://openalex.org/W3003455884","https://openalex.org/W3016541247","https://openalex.org/W3028443373","https://openalex.org/W3029443186","https://openalex.org/W3033408927","https://openalex.org/W3083842030","https://openalex.org/W3087734787","https://openalex.org/W3092874611","https://openalex.org/W3094015473","https://openalex.org/W3099584446","https://openalex.org/W3116903175","https://openalex.org/W3120395536","https://openalex.org/W3133656029","https://openalex.org/W3177270159","https://openalex.org/W3211777249","https://openalex.org/W4200456997","https://openalex.org/W4205349099","https://openalex.org/W4206441462","https://openalex.org/W4211181082","https://openalex.org/W4213090984","https://openalex.org/W4221099766","https://openalex.org/W4230250225","https://openalex.org/W4239909494","https://openalex.org/W4309513584","https://openalex.org/W6754431186"],"related_works":["https://openalex.org/W4307635350","https://openalex.org/W4297457437","https://openalex.org/W4387060260","https://openalex.org/W4285592078","https://openalex.org/W4408566947","https://openalex.org/W4415302609","https://openalex.org/W4407136850","https://openalex.org/W4385951306","https://openalex.org/W4321179060","https://openalex.org/W4384030489"],"abstract_inverted_index":{"Background":[0],"Lower":[1],"respiratory":[2,41],"tract":[3],"infections":[4,239],"are":[5],"the":[6,45,61,145,151,158,167,186,199,205,213,226,231],"leading":[7],"cause":[8],"of":[9,34,48,63,96,108,119,131,147,154,178,201,207,215,233,252],"morbidity":[10],"and":[11,17,27,37,74,138,170,181,189],"mortality":[12],"in":[13,80,83,157,166,185,236,240],"children":[14,58,241],"worldwide.":[15],"Timely":[16],"accurate":[18,46],"pathogen":[19],"detection":[20,32,47,152,176,194],"is":[21,225],"crucial":[22],"for":[23],"proper":[24],"clinical":[25],"diagnosis":[26],"therapeutic":[28],"strategies.":[29],"The":[30,94,115,174],"low":[31,38],"efficiency":[33],"conventional":[35,87],"methods":[36],"specificity":[39,95],"using":[40,68],"samples":[42],"seriously":[43],"hindered":[44],"pathogens.":[49],"Methods":[50],"In":[51,85],"this":[52],"study,":[53],"we":[54],"retrospectively":[55],"enrolled":[56],"1,032":[57],"to":[59,220,229],"evaluate":[60,230],"performance":[62,232],"metagenomics":[64],"next-generation":[65],"sequencing":[66],"(mNGS)":[67],"bronchoalveolar":[69],"lavage":[70],"fluid":[71],"(BALF)":[72],"sample":[73,79],"protected":[75],"bronchial":[76],"brushing":[77],"(BB)":[78],"diagnosing":[81,237],"pneumonia":[82],"children.":[84],"addition,":[86],"tests":[88],"(CTs)":[89],"were":[90,183],"also":[91],"performed.":[92],"Results":[93],"BB":[97,120,234,253],"mNGS":[98,110,121,133,193,221,235,254],"[67.3%":[99],"(95%":[100,112,123,135,141],"CI":[101,113,124,136,142],"58.6%\u201375.9%)]":[102],"was":[103,126,162],"significantly":[104],"higher":[105,128,163],"than":[106,129,164],"that":[107,130,165],"BALF":[109,132],"[38.5%":[111,140],"12.0%\u201364.9%)].":[114],"total":[116],"coincidence":[117],"rate":[118,153],"[77.6%":[122],"74.8%\u201380.5%)]":[125],"slightly":[127],"[76.5%":[134],"68.8%\u201384.1%)]":[137],"CTs":[139],"35.2%\u201341.9%)].":[143],"During":[144],"epidemics":[146],"Mycoplasma":[148],"pneumoniae":[149,156],",":[150],"M.":[155],">6-year":[159],"group":[160],"(81.8%)":[161],"3\u20136-year":[168,190],"(78.9%)":[169],"<3-year":[171],"groups":[172],"(21.5%).":[173],"highest":[175],"rates":[177],"bacteria,":[179],"fungi,":[180],"viruses":[182],"found":[184],"<3-year,":[187],">6-year,":[188],"groups,":[191],"respectively.":[192],"should":[195],"be":[196,256],"performed":[197],"at":[198,212],"duration":[200,214],"5\u20137":[202],"days":[203,217],"after":[204],"start":[206],"continuous":[208],"anti-microbial":[209],"therapy":[210],"or":[211],"6\u20139":[216],"from":[218],"onset":[219],"test.":[222],"Conclusions":[223],"This":[224],"first":[227],"report":[228],"pulmonary":[238],"on":[242,247],"a":[243],"large":[244],"scale.":[245],"Based":[246],"our":[248],"findings,":[249],"extensive":[250],"application":[251],"could":[255],"expected.":[257]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":6},{"year":2024,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2023-08-12T00:00:00"}