{"id":"https://openalex.org/W4385685801","doi":"https://doi.org/10.4049/jimmunol.210.supp.167.19","title":"UBL5 Modulates Expression of Crucial TLR Pathway Genes and Inflammasome Activation in Human Macrophage Cells","display_name":"UBL5 Modulates Expression of Crucial TLR Pathway Genes and Inflammasome Activation in Human Macrophage Cells","publication_year":2023,"publication_date":"2023-05-01","ids":{"openalex":"https://openalex.org/W4385685801","doi":"https://doi.org/10.4049/jimmunol.210.supp.167.19"},"language":"en","primary_location":{"id":"doi:10.4049/jimmunol.210.supp.167.19","doi":"https://doi.org/10.4049/jimmunol.210.supp.167.19","is_oa":true,"landing_page_url":"https://doi.org/10.4049/jimmunol.210.supp.167.19","pdf_url":"https://journals.aai.org/jimmunol/article-pdf/210/1_Supplement/167.19/1634308/167_19.pdf","source":{"id":"https://openalex.org/S38008053","display_name":"The Journal of Immunology","issn_l":"0022-1767","issn":["0022-1767","1550-6606"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4320800377","host_organization_name":"American Association of Immunologists","host_organization_lineage":["https://openalex.org/P4320800377"],"host_organization_lineage_names":["American Association of Immunologists"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Immunology","raw_type":"journal-article"},"volume":null,"type":"conference-abstract","is_traditional":false,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://journals.aai.org/jimmunol/article-pdf/210/1_Supplement/167.19/1634308/167_19.pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5017515840","display_name":"Sinu P. John","orcid":"https://orcid.org/0000-0003-0972-6599","observed_orcids":["https://orcid.org/0000-0003-0972-6599"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210134534","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Sinu P John","raw_affiliation_strings":["NIAID, NIH","1NIAID, NIH"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"NIAID, NIH","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210134534"]},{"raw_affiliation_string":"1NIAID, NIH","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5037551790","display_name":"Makheni Jean-Pierre","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210134534","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Makheni Jean-Pierre","raw_affiliation_strings":["NIAID, NIH","1NIAID, NIH"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"NIAID, NIH","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210134534"]},{"raw_affiliation_string":"1NIAID, NIH","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Samuel Katz","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210134534","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Samuel Katz","raw_affiliation_strings":["NIAID, NIH","1NIAID, NIH"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"NIAID, NIH","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210134534"]},{"raw_affiliation_string":"1NIAID, NIH","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5041569902","display_name":"Iain D. C. Fraser","orcid":"https://orcid.org/0000-0003-2272-3907","observed_orcids":["https://orcid.org/0000-0003-2272-3907"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210134534","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Iain D C Fraser","raw_affiliation_strings":["NIAID, National Institutes of Health","2NIAID, National Institutes of Health"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"NIAID, National Institutes of Health","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210134534"]},{"raw_affiliation_string":"2NIAID, National Institutes of Health","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3500,"currency":"USD","value_usd":3500},"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"210","issue":"Supplement_1","first_page":"167.19","last_page":"167.19"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10371","display_name":"Immune Response and Inflammation","score":0.9140999913215637,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10371","display_name":"Immune Response and Inflammation","score":0.9140999913215637,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11078","display_name":"Inflammasome and immune disorders","score":0.07649999856948853,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":0.00279999990016222,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/splicing-regulators","display_name":"splicing regulators","score":0.7599999904632568},{"id":"https://openalex.org/keywords/tlr4-signaling","display_name":"TLR4 signaling","score":0.7179999947547913},{"id":"https://openalex.org/keywords/lps-induced-gene-expression","display_name":"LPS-induced gene expression","score":0.7419999837875366},{"id":"https://openalex.org/keywords/tnf-expression","display_name":"TNF expression","score":0.8050000071525574},{"id":"https://openalex.org/keywords/macrophages","display_name":"macrophages","score":0.8090000152587891},{"id":"https://openalex.org/keywords/alternative-splicing","display_name":"alternative splicing","score":0.6919999718666077},{"id":"https://openalex.org/keywords/sirna-screen","display_name":"siRNA screen","score":0.8080000281333923},{"id":"https://openalex.org/keywords/innate-immunity","display_name":"innate immunity","score":0.414000004529953}],"concepts":[{"id":"https://openalex.org/C40000846","wikidata":"https://www.wikidata.org/wiki/Q915868","display_name":"Spliceosome","level":5,"score":0.7685192823410034},{"id":"https://openalex.org/C54458228","wikidata":"https://www.wikidata.org/wiki/Q237218","display_name":"RNA splicing","level":4,"score":0.7548841238021851},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6497223377227783},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.6058352589607239},{"id":"https://openalex.org/C6929976","wikidata":"https://www.wikidata.org/wiki/Q3771881","display_name":"Regulator","level":3,"score":0.5978743433952332},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5714422464370728},{"id":"https://openalex.org/C194583182","wikidata":"https://www.wikidata.org/wiki/Q21114084","display_name":"Alternative splicing","level":4,"score":0.4498691260814667},{"id":"https://openalex.org/C2776070231","wikidata":"https://www.wikidata.org/wiki/Q14914098","display_name":"TLR4","level":3,"score":0.4404789209365845},{"id":"https://openalex.org/C2777209026","wikidata":"https://www.wikidata.org/wiki/Q412405","display_name":"Inflammasome","level":3,"score":0.42721027135849},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.42534738779067993},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3772740066051483},{"id":"https://openalex.org/C62478195","wikidata":"https://www.wikidata.org/wiki/Q828130","display_name":"Signal transduction","level":2,"score":0.36637410521507263},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.34053850173950195},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.28439393639564514},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.17717835307121277},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.4049/jimmunol.210.supp.167.19","doi":"https://doi.org/10.4049/jimmunol.210.supp.167.19","is_oa":true,"landing_page_url":"https://doi.org/10.4049/jimmunol.210.supp.167.19","pdf_url":"https://journals.aai.org/jimmunol/article-pdf/210/1_Supplement/167.19/1634308/167_19.pdf","source":{"id":"https://openalex.org/S38008053","display_name":"The Journal of Immunology","issn_l":"0022-1767","issn":["0022-1767","1550-6606"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4320800377","host_organization_name":"American Association of Immunologists","host_organization_lineage":["https://openalex.org/P4320800377"],"host_organization_lineage_names":["American Association of Immunologists"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Immunology","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.4049/jimmunol.210.supp.167.19","doi":"https://doi.org/10.4049/jimmunol.210.supp.167.19","is_oa":true,"landing_page_url":"https://doi.org/10.4049/jimmunol.210.supp.167.19","pdf_url":"https://journals.aai.org/jimmunol/article-pdf/210/1_Supplement/167.19/1634308/167_19.pdf","source":{"id":"https://openalex.org/S38008053","display_name":"The Journal of Immunology","issn_l":"0022-1767","issn":["0022-1767","1550-6606"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4320800377","host_organization_name":"American Association of Immunologists","host_organization_lineage":["https://openalex.org/P4320800377"],"host_organization_lineage_names":["American Association of Immunologists"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Immunology","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.8371999859809875,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4385685801.pdf","grobid_xml":"https://content.openalex.org/works/W4385685801.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2593476978","https://openalex.org/W2594802421","https://openalex.org/W4313360145","https://openalex.org/W7019370577","https://openalex.org/W1514955262","https://openalex.org/W7204834667","https://openalex.org/W2070044709","https://openalex.org/W2085963619","https://openalex.org/W1536422556","https://openalex.org/W1975095833"],"abstract_inverted_index":{"Abstract":[0],"We":[1,15,105],"conducted":[2,106],"a":[3,17,39,48,55,60,66,86],"genome-wide":[4],"siRNA":[5],"screen":[6,35,57],"for":[7,20,42,170],"regulators":[8],"of":[9,88,93,122,132,141,167,174,181,186,201,217,223],"LPS-driven":[10,70],"TNF":[11],"expression":[12,144,169],"in":[13,69,81,102,118,162,183,205],"macrophages.":[14],"used":[16],"splicing-independent":[18],"reporter":[19],"the":[21,33,82,94,119,126,133,139,153,163,175,184,194,213],"screen,":[22],"and":[23,59,109,113,165,199],"while":[24],"we":[25,37,114],"identified":[26],"very":[27],"few":[28],"core":[29,91,172],"spliceosome":[30],"components":[31,92,131,173],"among":[32],"strongest":[34],"hits,":[36],"observed":[38,115,137],"clear":[40],"enrichment":[41],"peripheral":[43],"splicing":[44,51,121,155],"regulators.":[45],"Among":[46],"these,":[47],"novel":[49],"putative":[50,154],"regulator,":[52],"UBL5,":[53],"gave":[54],"strong":[56],"phenotype,":[58],"UBL5":[61,83,157,182],"shRNA-expressing":[62],"cell":[63],"line":[64],"showed":[65],"selective":[67],"defect":[68],"gene":[71,143,168],"expression.":[72],"While":[73],"many":[74],"genes":[75],"were":[76,99],"induced":[77],"normally":[78],"by":[79,193,212],"LPS":[80],"deficient":[84],"cells,":[85],"subset":[87],"genes,":[89,124],"including":[90],"LPS-activated":[95],"TLR4":[96,134,176,187],"signaling":[97,177,188],"pathway,":[98],"substantially":[100],"diminished":[101],"UBL5-deficient":[103,206],"cells.":[104,207],"both":[107],"Illumina":[108],"Pacbio":[110],"long-read":[111],"sequencing,":[112],"marked":[116],"defects":[117],"LPS-induced":[120],"numerous":[123],"however":[125],"primary":[127],"impact":[128],"on":[129],"known":[130],"pathway":[135,189],"was":[136,190,210],"at":[138],"level":[140],"overall":[142],"rather":[145],"than":[146],"alternative":[147],"splicing.":[148],"Our":[149],"data":[150],"suggests":[151],"that":[152],"regulator":[156],"also":[158],"has":[159],"key":[160],"roles":[161],"maintenance":[164],"induction":[166,185],"several":[171],"pathway.":[178],"Key":[179],"role":[180],"further":[191],"evidenced":[192],"defective":[195],"NLRP3":[196],"inflammasome":[197],"activation":[198],"protection":[200],"OC43":[202],"coronavirus":[203],"infection":[204],"This":[208],"work":[209],"supported":[211],"Intramural":[214,220],"Research":[215,221],"Program":[216,222],"NIAID,":[218,224],"NIH":[219,225]},"counts_by_year":[],"updated_date":"2026-09-29T14:09:16.559479","created_date":"2023-08-10T00:00:00"}