{"id":"https://openalex.org/W4385346181","doi":"https://doi.org/10.1201/9781003456100-3","title":"Advances in the Field of Single-Particle Cryo-Electron Microscopy Over the Last Decade","display_name":"Advances in the Field of Single-Particle Cryo-Electron Microscopy Over the Last Decade","publication_year":2023,"publication_date":"2023-07-28","ids":{"openalex":"https://openalex.org/W4385346181","doi":"https://doi.org/10.1201/9781003456100-3"},"language":"en","primary_location":{"id":"doi:10.1201/9781003456100-3","doi":"https://doi.org/10.1201/9781003456100-3","is_oa":false,"landing_page_url":"https://doi.org/10.1201/9781003456100-3","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Novel Developments in Cryo\u2010EM of Biological Molecules","raw_type":"book-chapter"},"volume":{"id":"https://openalex.org/W4385345133","display_name":"Novel Developments in Cryo\u2010EM of Biological Molecules"},"type":"book-chapter","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5042892654","display_name":"Joachim Frank","orcid":"https://orcid.org/0000-0001-5449-6943","observed_orcids":["https://orcid.org/0000-0001-5449-6943","https://orcid.org/0000-0002-2442-1112"]},"institutions":[{"id":"https://openalex.org/I78577930","display_name":"Columbia University","ror":"https://ror.org/00hj8s172","country_code":"US","type":"education","lineage":["https://openalex.org/I78577930"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Joachim Frank","raw_affiliation_strings":["Departments of Biochemistry and Molecular Biophysics and of Biological Sciences, Columbia University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Biochemistry and Molecular Biophysics and of Biological Sciences, Columbia University","institution_ids":["https://openalex.org/I78577930"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5042892654"],"corresponding_institution_ids":["https://openalex.org/I78577930"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.25,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":"25","last_page":"35"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10857","display_name":"Advanced Electron Microscopy Techniques and Applications","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10857","display_name":"Advanced Electron Microscopy Techniques and Applications","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/single-particle-cryo-electron-microscopy","display_name":"single-particle cryo-electron microscopy","score":0.9950000047683716},{"id":"https://openalex.org/keywords/ribosome-structure","display_name":"ribosome structure","score":0.8510000109672546},{"id":"https://openalex.org/keywords/asymmetric-structures","display_name":"asymmetric structures","score":0.36899998784065247},{"id":"https://openalex.org/keywords/icosahedral-symmetry","display_name":"icosahedral symmetry","score":0.7229999899864197},{"id":"https://openalex.org/keywords/recording-media","display_name":"recording media","score":0.5419999957084656},{"id":"https://openalex.org/keywords/throughput","display_name":"throughput","score":0.3619999885559082},{"id":"https://openalex.org/keywords/standardization","display_name":"standardization","score":0.7289999723434448},{"id":"https://openalex.org/keywords/automation","display_name":"automation","score":0.8889999985694885}],"concepts":[{"id":"https://openalex.org/C93877712","wikidata":"https://www.wikidata.org/wiki/Q132560","display_name":"Electron microscope","level":2,"score":0.5767074823379517},{"id":"https://openalex.org/C2778517922","wikidata":"https://www.wikidata.org/wiki/Q7140482","display_name":"Particle (ecology)","level":2,"score":0.4686388671398163},{"id":"https://openalex.org/C65293305","wikidata":"https://www.wikidata.org/wiki/Q7523967","display_name":"Single particle analysis","level":3,"score":0.4628787040710449},{"id":"https://openalex.org/C9652623","wikidata":"https://www.wikidata.org/wiki/Q190109","display_name":"Field (mathematics)","level":2,"score":0.44025492668151855},{"id":"https://openalex.org/C147080431","wikidata":"https://www.wikidata.org/wiki/Q1074953","display_name":"Microscopy","level":2,"score":0.4120887815952301},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.3988783359527588},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.3296399414539337},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.2591390013694763},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.22729945182800293},{"id":"https://openalex.org/C127313418","wikidata":"https://www.wikidata.org/wiki/Q1069","display_name":"Geology","level":0,"score":0.19617947936058044},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.09197264909744263},{"id":"https://openalex.org/C153294291","wikidata":"https://www.wikidata.org/wiki/Q25261","display_name":"Meteorology","level":1,"score":0.05070006847381592},{"id":"https://openalex.org/C111368507","wikidata":"https://www.wikidata.org/wiki/Q43518","display_name":"Oceanography","level":1,"score":0.0},{"id":"https://openalex.org/C202444582","wikidata":"https://www.wikidata.org/wiki/Q837863","display_name":"Pure mathematics","level":1,"score":0.0},{"id":"https://openalex.org/C2779345167","wikidata":"https://www.wikidata.org/wiki/Q104541","display_name":"Aerosol","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1201/9781003456100-3","doi":"https://doi.org/10.1201/9781003456100-3","is_oa":false,"landing_page_url":"https://doi.org/10.1201/9781003456100-3","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Novel Developments in Cryo\u2010EM of Biological Molecules","raw_type":"book-chapter"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2212589734","https://openalex.org/W4385348687","https://openalex.org/W2998197581","https://openalex.org/W2160559764","https://openalex.org/W2129664916","https://openalex.org/W2536051193","https://openalex.org/W2778717545","https://openalex.org/W4375948752","https://openalex.org/W2252598630","https://openalex.org/W2763740855"],"abstract_inverted_index":{"In":[0],"single-particle":[1,66],"cryo-electron":[2],"microscopy":[3],"(cryo-EM),":[4],"molecules":[5],"suspended":[6],"in":[7,20,37,86,103,113],"a":[8,82,87],"thin":[9],"aqueous":[10],"layer":[11],"are":[12],"rapidly":[13],"frozen":[14],"and":[15,122],"imaged":[16],"at":[17,43,55],"cryogenic":[18],"temperature":[19],"the":[21,31,38,48,63,74,91,104,109,114,127],"transmission":[22],"electron":[23],"microscope.":[24],"This":[25],"chapter":[26],"discusses":[27],"technological":[28],"progress":[29],"over":[30],"past":[32,105],"decade,":[33,83],"which":[34],"has":[35,99],"culminated":[36],"determination":[39],"of":[40,50,65,69,94,116,130],"ribosome":[41,75],"structure":[42,49],"2.5-\u00c5":[44],"resolution.":[45],"Even":[46],"though":[47],"viruses":[51],"could":[52],"be":[53],"solved":[54],"near-3-\u00c5":[56],"resolution":[57,64],"thanks":[58],"to":[59,138],"their":[60],"icosahedral":[61],"symmetry,":[62],"cryo-EM":[67],"reconstructions":[68],"asymmetric":[70],"structures":[71],"such":[72],"as":[73],"showed":[76],"little":[77],"improvement":[78],"for":[79],"more":[80],"than":[81],"being":[84],"limited":[85],"fundamental":[88],"way":[89],"by":[90,133],"poor":[92],"quality":[93],"traditional":[95],"recording":[96],"media.":[97],"What":[98],"been":[100],"particularly":[101],"evident":[102],"3":[106],"years":[107,129],"is":[108],"much":[110],"accelerated":[111],"pace":[112],"development":[115],"techniques":[117],"toward":[118],"higher":[119],"throughput,":[120],"standardization":[121],"automation,":[123],"inviting":[124],"comparisons":[125],"with":[126],"early":[128],"X-ray":[131],"crystallography":[132],"those":[134],"practitioners":[135],"old":[136],"enough":[137],"remember.":[139]},"counts_by_year":[],"updated_date":"2026-10-10T09:11:00.788812","created_date":"2023-07-29T00:00:00"}