{"id":"https://openalex.org/W4385197961","doi":"https://doi.org/10.1016/j.crmeth.2023.100541","title":"Base-editing screens illuminate variant effects in human hematopoiesis","display_name":"Base-editing screens illuminate variant effects in human hematopoiesis","publication_year":2023,"publication_date":"2023-07-01","ids":{"openalex":"https://openalex.org/W4385197961","doi":"https://doi.org/10.1016/j.crmeth.2023.100541","pmid":"https://pubmed.ncbi.nlm.nih.gov/37533644","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/10391558"},"language":"en","primary_location":{"id":"doi:10.1016/j.crmeth.2023.100541","doi":"https://doi.org/10.1016/j.crmeth.2023.100541","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.crmeth.2023.100541","pdf_url":null,"source":{"id":"https://openalex.org/S4210214705","display_name":"Cell Reports Methods","issn_l":"2667-2375","issn":["2667-2375"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-20"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cell Reports Methods","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.1016/j.crmeth.2023.100541","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5065147379","display_name":"Alina Vaitsiankova","orcid":"https://orcid.org/0000-0002-9355-0903","observed_orcids":["https://orcid.org/0000-0002-9355-0903"]},"institutions":[{"id":"https://openalex.org/I2799503643","display_name":"Columbia University Irving Medical Center","ror":"https://ror.org/01esghr10","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I2799503643"]},{"id":"https://openalex.org/I4390039230","display_name":"Herbert Irving Comprehensive Cancer Center","ror":"https://ror.org/051kc1939","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4390039230"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Alina Vaitsiankova","raw_affiliation_strings":["Department of Genetics and Development, Institute for Cancer Genetics, Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center, New York, NY 10032, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Genetics and Development, Institute for Cancer Genetics, Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center, New York, NY 10032, USA","institution_ids":["https://openalex.org/I2799503643","https://openalex.org/I4390039230"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5027942305","display_name":"Tanay Thakar","orcid":"https://orcid.org/0000-0001-5261-458X","observed_orcids":["https://orcid.org/0000-0001-5261-458X"]},"institutions":[{"id":"https://openalex.org/I2799503643","display_name":"Columbia University Irving Medical Center","ror":"https://ror.org/01esghr10","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I2799503643"]},{"id":"https://openalex.org/I4390039230","display_name":"Herbert Irving Comprehensive Cancer Center","ror":"https://ror.org/051kc1939","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4390039230"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Tanay Thakar","raw_affiliation_strings":["Department of Genetics and Development, Institute for Cancer Genetics, Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center, New York, NY 10032, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Genetics and Development, Institute for Cancer Genetics, Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center, New York, NY 10032, USA","institution_ids":["https://openalex.org/I2799503643","https://openalex.org/I4390039230"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5024098581","display_name":"Alberto Ciccia","orcid":"https://orcid.org/0000-0003-4789-6564","observed_orcids":["https://orcid.org/0000-0003-4789-6564"]},"institutions":[{"id":"https://openalex.org/I2799503643","display_name":"Columbia University Irving Medical Center","ror":"https://ror.org/01esghr10","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I2799503643"]},{"id":"https://openalex.org/I4390039230","display_name":"Herbert Irving Comprehensive Cancer Center","ror":"https://ror.org/051kc1939","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4390039230"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Alberto Ciccia","raw_affiliation_strings":["Department of Genetics and Development, Institute for Cancer Genetics, Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center, New York, NY 10032, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Genetics and Development, Institute for Cancer Genetics, Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center, New York, NY 10032, USA","institution_ids":["https://openalex.org/I2799503643","https://openalex.org/I4390039230"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5024098581"],"corresponding_institution_ids":["https://openalex.org/I2799503643","https://openalex.org/I4390039230"],"apc_list":{"value":5200,"currency":"USD","value_usd":5200},"apc_paid":{"value":5200,"currency":"USD","value_usd":5200},"fwci":0.0865,"has_fulltext":true,"cited_by_count":1,"citation_normalized_percentile":{"value":0.30068254,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":92,"max":95},"biblio":{"volume":"3","issue":"7","first_page":"100541","last_page":"100541"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9824000000953674,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9824000000953674,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12610","display_name":"RNA regulation and disease","score":0.003599999938160181,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/base-editing","display_name":"base editing","score":0.9629999995231628},{"id":"https://openalex.org/keywords/crispr-screening","display_name":"CRISPR screen","score":0.984000027179718},{"id":"https://openalex.org/keywords/hematopoiesis","display_name":"hematopoiesis","score":0.8240000009536743},{"id":"https://openalex.org/keywords/hematologic-disorders","display_name":"hematologic disorders","score":0.8009999990463257},{"id":"https://openalex.org/keywords/high-throughput-screening","display_name":"high-throughput screening","score":0.7889999747276306},{"id":"https://openalex.org/keywords/genome-editing","display_name":"genome editing","score":0.3490000069141388}],"concepts":[{"id":"https://openalex.org/C98108389","wikidata":"https://www.wikidata.org/wiki/Q412563","display_name":"CRISPR","level":3,"score":0.7308675050735474},{"id":"https://openalex.org/C2779343474","wikidata":"https://www.wikidata.org/wiki/Q3109175","display_name":"Context (archaeology)","level":2,"score":0.6146413683891296},{"id":"https://openalex.org/C109159458","wikidata":"https://www.wikidata.org/wiki/Q919283","display_name":"Haematopoiesis","level":3,"score":0.5868082046508789},{"id":"https://openalex.org/C42058472","wikidata":"https://www.wikidata.org/wiki/Q810214","display_name":"Base (topology)","level":2,"score":0.5861765742301941},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.5579817295074463},{"id":"https://openalex.org/C144501496","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome editing","level":4,"score":0.45140162110328674},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.41918280720710754},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.33621570467948914},{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.3147513270378113},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.15131980180740356},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.08327165246009827},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.06222841143608093},{"id":"https://openalex.org/C134306372","wikidata":"https://www.wikidata.org/wiki/Q7754","display_name":"Mathematical analysis","level":1,"score":0.0},{"id":"https://openalex.org/C151730666","wikidata":"https://www.wikidata.org/wiki/Q7205","display_name":"Paleontology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006410","descriptor_name":"Hematopoiesis","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1016/j.crmeth.2023.100541","doi":"https://doi.org/10.1016/j.crmeth.2023.100541","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.crmeth.2023.100541","pdf_url":null,"source":{"id":"https://openalex.org/S4210214705","display_name":"Cell Reports Methods","issn_l":"2667-2375","issn":["2667-2375"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-20"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cell Reports Methods","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:10391558","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/10391558","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC10391558/pdf/main.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cell Rep Methods","raw_type":"Text"},{"id":"pmid:37533644","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/37533644","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cell reports methods","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1016/j.crmeth.2023.100541","doi":"https://doi.org/10.1016/j.crmeth.2023.100541","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.crmeth.2023.100541","pdf_url":null,"source":{"id":"https://openalex.org/S4210214705","display_name":"Cell Reports Methods","issn_l":"2667-2375","issn":["2667-2375"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-20"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cell Reports Methods","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.6240000128746033}],"study_designs":[],"awards":[{"id":"https://openalex.org/G9000897136","display_name":"Regulation of DNA synthesis in response to DNA damage","funder_award_id":"R01CA197774","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4385197961.pdf","grobid_xml":"https://content.openalex.org/works/W4385197961.grobid-xml"},"referenced_works_count":14,"referenced_works":["https://openalex.org/W3035539198","https://openalex.org/W3035764705","https://openalex.org/W3131839988","https://openalex.org/W3132864675","https://openalex.org/W3158339810","https://openalex.org/W3192108213","https://openalex.org/W3204120268","https://openalex.org/W4206238555","https://openalex.org/W4211123163","https://openalex.org/W4211219428","https://openalex.org/W4367692380","https://openalex.org/W4368363424","https://openalex.org/W4380563796","https://openalex.org/W4380786202"],"related_works":["https://openalex.org/W4380364057","https://openalex.org/W4367692380","https://openalex.org/W3014628987","https://openalex.org/W3169142762","https://openalex.org/W4313482030","https://openalex.org/W4415143435","https://openalex.org/W3011549593","https://openalex.org/W3130400928","https://openalex.org/W4395007210","https://openalex.org/W3097318701"],"abstract_inverted_index":{",":[0],"Martin-Rufino":[1],"et":[2],"al.":[3],"develop":[4],"a":[5,24],"strategy":[6],"for":[7,37],"performing":[8],"high-throughput":[9],"base-editing":[10,35],"CRISPR":[11],"screens":[12,36],"coupled":[13],"with":[14],"single-cell":[15],"readouts":[16],"in":[17],"the":[18,29,32,38],"context":[19],"of":[20,26,34,42],"human":[21],"hematopoiesis.":[22],"Through":[23],"series":[25],"proof-of-principle":[27],"experiments,":[28],"authors":[30],"demonstrate":[31],"potential":[33],"study":[39],"and":[40],"treatment":[41],"hematological":[43],"disorders.":[44]},"counts_by_year":[{"year":2026,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2023-07-25T00:00:00"}