{"id":"https://openalex.org/W4384120292","doi":"https://doi.org/10.1021/acs.langmuir.3c00648","title":"Acetylcholinesterase Adsorption on Modified Gold: Effect of Surface Chemistry on Enzyme Binding and Activity","display_name":"Acetylcholinesterase Adsorption on Modified Gold: Effect of Surface Chemistry on Enzyme Binding and Activity","publication_year":2023,"publication_date":"2023-07-13","ids":{"openalex":"https://openalex.org/W4384120292","doi":"https://doi.org/10.1021/acs.langmuir.3c00648","pmid":"https://pubmed.ncbi.nlm.nih.gov/37439709"},"language":"en","primary_location":{"id":"doi:10.1021/acs.langmuir.3c00648","doi":"https://doi.org/10.1021/acs.langmuir.3c00648","is_oa":false,"landing_page_url":"https://doi.org/10.1021/acs.langmuir.3c00648","pdf_url":null,"source":{"id":"https://openalex.org/S75741262","display_name":"Langmuir","issn_l":"0743-7463","issn":["0743-7463","1520-5827"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-d","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Langmuir","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5003848518","display_name":"Joshua M. Correira","orcid":"https://orcid.org/0000-0002-2971-4619","observed_orcids":["https://orcid.org/0000-0002-2971-4619"]},"institutions":[{"id":"https://openalex.org/I4405271599","display_name":"Texas Materials Institute","ror":"https://ror.org/02q1tb011","country_code":"US","type":"education","lineage":["https://openalex.org/I4405271599"]},{"id":"https://openalex.org/I86519309","display_name":"The University of Texas at Austin","ror":"https://ror.org/00hj54h04","country_code":"US","type":"education","lineage":["https://openalex.org/I86519309"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Joshua M. Correira","raw_affiliation_strings":["Department of Chemistry, Texas Materials Institute, and Interdisciplinary Life Sciences Program, The University of Texas at Austin, 105 E 24th St. STOP A5300, Austin, Texas 78712-1224, United States"],"raw_orcid":"https://orcid.org/0000-0002-2971-4619","affiliations":[{"raw_affiliation_string":"Department of Chemistry, Texas Materials Institute, and Interdisciplinary Life Sciences Program, The University of Texas at Austin, 105 E 24th St. STOP A5300, Austin, Texas 78712-1224, United States","institution_ids":["https://openalex.org/I4405271599","https://openalex.org/I86519309"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5092457762","display_name":"Diane E. Madeksho","orcid":"https://orcid.org/0000-0003-3138-9664","observed_orcids":["https://orcid.org/0000-0003-3138-9664"]},"institutions":[{"id":"https://openalex.org/I4405271599","display_name":"Texas Materials Institute","ror":"https://ror.org/02q1tb011","country_code":"US","type":"education","lineage":["https://openalex.org/I4405271599"]},{"id":"https://openalex.org/I86519309","display_name":"The University of Texas at Austin","ror":"https://ror.org/00hj54h04","country_code":"US","type":"education","lineage":["https://openalex.org/I86519309"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Diane E. Madeksho","raw_affiliation_strings":["Department of Chemistry, Texas Materials Institute, and Interdisciplinary Life Sciences Program, The University of Texas at Austin, 105 E 24th St. STOP A5300, Austin, Texas 78712-1224, United States"],"raw_orcid":"https://orcid.org/0000-0003-3138-9664","affiliations":[{"raw_affiliation_string":"Department of Chemistry, Texas Materials Institute, and Interdisciplinary Life Sciences Program, The University of Texas at Austin, 105 E 24th St. STOP A5300, Austin, Texas 78712-1224, United States","institution_ids":["https://openalex.org/I4405271599","https://openalex.org/I86519309"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5050572384","display_name":"Lauren J. Webb","orcid":"https://orcid.org/0000-0001-9999-5500","observed_orcids":["https://orcid.org/0000-0001-9999-5500"]},"institutions":[{"id":"https://openalex.org/I4405271599","display_name":"Texas Materials Institute","ror":"https://ror.org/02q1tb011","country_code":"US","type":"education","lineage":["https://openalex.org/I4405271599"]},{"id":"https://openalex.org/I86519309","display_name":"The University of Texas at Austin","ror":"https://ror.org/00hj54h04","country_code":"US","type":"education","lineage":["https://openalex.org/I86519309"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Lauren J. Webb","raw_affiliation_strings":["Department of Chemistry, Texas Materials Institute, and Interdisciplinary Life Sciences Program, The University of Texas at Austin, 105 E 24th St. STOP A5300, Austin, Texas 78712-1224, United States"],"raw_orcid":"https://orcid.org/0000-0001-9999-5500","affiliations":[{"raw_affiliation_string":"Department of Chemistry, Texas Materials Institute, and Interdisciplinary Life Sciences Program, The University of Texas at Austin, 105 E 24th St. STOP A5300, Austin, Texas 78712-1224, United States","institution_ids":["https://openalex.org/I4405271599","https://openalex.org/I86519309"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5050572384"],"corresponding_institution_ids":["https://openalex.org/I4405271599","https://openalex.org/I86519309"],"apc_list":{"value":5000,"currency":"USD","value_usd":5000},"apc_paid":null,"fwci":0.6218,"has_fulltext":false,"cited_by_count":7,"citation_normalized_percentile":{"value":0.60134381,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":90,"max":97},"biblio":{"volume":"39","issue":"29","first_page":"9973","last_page":"9979"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.8125,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.8125,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10859","display_name":"Polymer Surface Interaction Studies","score":0.15369999408721924,"subfield":{"id":"https://openalex.org/subfields/2508","display_name":"Surfaces, Coatings and Films"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10212","display_name":"Electrochemical sensors and biosensors","score":0.010599999688565731,"subfield":{"id":"https://openalex.org/subfields/2208","display_name":"Electrical and Electronic Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/acetylcholinesterase","display_name":"acetylcholinesterase","score":1.0},{"id":"https://openalex.org/keywords/enzyme-adsorption","display_name":"enzyme adsorption","score":0.9259999990463257},{"id":"https://openalex.org/keywords/gold-surfaces","display_name":"gold surfaces","score":0.6449999809265137},{"id":"https://openalex.org/keywords/self-assembled-monolayers","display_name":"self-assembled monolayers","score":0.921999990940094},{"id":"https://openalex.org/keywords/surface-hydrophobicity","display_name":"surface hydrophobicity","score":0.9850000143051147},{"id":"https://openalex.org/keywords/surface-charge","display_name":"surface charge","score":0.9779999852180481},{"id":"https://openalex.org/keywords/enzyme-activity-retention","display_name":"enzyme activity retention","score":0.8029999732971191},{"id":"https://openalex.org/keywords/enzyme-binding","display_name":"enzyme binding","score":0.5799999833106995},{"id":"https://openalex.org/keywords/helical-peptides","display_name":"helical peptides","score":0.7609999775886536},{"id":"https://openalex.org/keywords/surface-modification","display_name":"surface modification","score":0.4050000011920929},{"id":"https://openalex.org/keywords/enzyme-immobilization","display_name":"enzyme immobilization","score":0.6800000071525574},{"id":"https://openalex.org/keywords/surface-concentration","display_name":"surface concentration","score":0.3149999976158142}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.8373711705207825},{"id":"https://openalex.org/C7070889","wikidata":"https://www.wikidata.org/wiki/Q902488","display_name":"Monolayer","level":2,"score":0.8291267156600952},{"id":"https://openalex.org/C150394285","wikidata":"https://www.wikidata.org/wiki/Q180254","display_name":"Adsorption","level":2,"score":0.7938135862350464},{"id":"https://openalex.org/C2778816929","wikidata":"https://www.wikidata.org/wiki/Q22676679","display_name":"Acetylcholinesterase","level":3,"score":0.6894861459732056},{"id":"https://openalex.org/C131649739","wikidata":"https://www.wikidata.org/wiki/Q1121115","display_name":"Self-assembled monolayer","level":3,"score":0.6429000496864319},{"id":"https://openalex.org/C2780358262","wikidata":"https://www.wikidata.org/wiki/Q1540423","display_name":"Surface charge","level":2,"score":0.5226202607154846},{"id":"https://openalex.org/C180577832","wikidata":"https://www.wikidata.org/wiki/Q127920","display_name":"Covalent bond","level":2,"score":0.49234235286712646},{"id":"https://openalex.org/C160756335","wikidata":"https://www.wikidata.org/wiki/Q669391","display_name":"Biosensor","level":2,"score":0.48755449056625366},{"id":"https://openalex.org/C117626034","wikidata":"https://www.wikidata.org/wiki/Q26336","display_name":"Electrostatics","level":2,"score":0.45578402280807495},{"id":"https://openalex.org/C115537861","wikidata":"https://www.wikidata.org/wiki/Q7645993","display_name":"Surface modification","level":2,"score":0.43710044026374817},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.41465532779693604},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.35114896297454834},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.3289063572883606},{"id":"https://openalex.org/C113196181","wikidata":"https://www.wikidata.org/wiki/Q485223","display_name":"Analytical Chemistry (journal)","level":2,"score":0.32313060760498047},{"id":"https://openalex.org/C147789679","wikidata":"https://www.wikidata.org/wiki/Q11372","display_name":"Physical chemistry","level":1,"score":0.20312020182609558},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.14207065105438232}],"mesh":[{"descriptor_ui":"D000110","descriptor_name":"Acetylcholinesterase","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000327","descriptor_name":"Adsorption","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006046","descriptor_name":"Gold","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D013499","descriptor_name":"Surface Properties","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D057927","descriptor_name":"Hydrophobic and Hydrophilic Interactions","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/acs.langmuir.3c00648","doi":"https://doi.org/10.1021/acs.langmuir.3c00648","is_oa":false,"landing_page_url":"https://doi.org/10.1021/acs.langmuir.3c00648","pdf_url":null,"source":{"id":"https://openalex.org/S75741262","display_name":"Langmuir","issn_l":"0743-7463","issn":["0743-7463","1520-5827"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-d","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Langmuir","raw_type":"journal-article"},{"id":"pmid:37439709","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/37439709","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Langmuir : the ACS journal of surfaces and colloids","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"score":0.4699999988079071,"id":"https://metadata.un.org/sdg/6","display_name":"Clean water and sanitation"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G3051044190","display_name":null,"funder_award_id":"CHE-1807215","funder_id":"https://openalex.org/F4320337393","funder_display_name":"Division of Chemistry"},{"id":"https://openalex.org/G6452690812","display_name":null,"funder_award_id":"CHE-2203414","funder_id":"https://openalex.org/F4320337393","funder_display_name":"Division of Chemistry"}],"funders":[{"id":"https://openalex.org/F4320337393","display_name":"Division of Chemistry","ror":"https://ror.org/01ar8dr59"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":35,"referenced_works":["https://openalex.org/W1979487244","https://openalex.org/W1982350871","https://openalex.org/W1983292451","https://openalex.org/W1993063222","https://openalex.org/W1993902535","https://openalex.org/W2006370393","https://openalex.org/W2008827477","https://openalex.org/W2009377140","https://openalex.org/W2026736219","https://openalex.org/W2035637496","https://openalex.org/W2044252957","https://openalex.org/W2047528317","https://openalex.org/W2047608438","https://openalex.org/W2047869488","https://openalex.org/W2055218251","https://openalex.org/W2057793439","https://openalex.org/W2063731290","https://openalex.org/W2088928060","https://openalex.org/W2115432877","https://openalex.org/W2131964034","https://openalex.org/W2155963087","https://openalex.org/W2166806803","https://openalex.org/W2333557439","https://openalex.org/W2416532170","https://openalex.org/W2478731157","https://openalex.org/W2588345574","https://openalex.org/W2725295997","https://openalex.org/W2731407107","https://openalex.org/W2881328687","https://openalex.org/W2895739481","https://openalex.org/W2912761569","https://openalex.org/W3004079430","https://openalex.org/W3040831456","https://openalex.org/W4220999748","https://openalex.org/W4283461683"],"related_works":["https://openalex.org/W6927305413","https://openalex.org/W4220999748","https://openalex.org/W2026736219","https://openalex.org/W4311187465","https://openalex.org/W2562620884","https://openalex.org/W2007715802","https://openalex.org/W2795677890","https://openalex.org/W2416532170","https://openalex.org/W3004079430","https://openalex.org/W2306542306"],"abstract_inverted_index":{"Surface":[0,48],"chemistry":[1],"plays":[2],"a":[3,18,144,178,192,239,247],"crucial":[4],"role":[5],"in":[6],"the":[7,26,34,74,86,93,128,135,167,182,216,227,235,259],"performance":[8],"of":[9,28,38,76,96,105,138,191,219,229,249,261],"biosensors":[10],"and":[11,31,36,62,78,147,205,252,272,277],"biocatalysts,":[12],"where":[13],"enzymes":[14],"directly":[15,113],"interact":[16],"with":[17,55,181,197],"solid":[19],"support.":[20],"In":[21],"this":[22,212],"work,":[23],"we":[24,91],"investigated":[25,73],"effect":[27],"surface":[29,94,101,110,116,142,155,175,228],"charge":[30],"hydrophobicity":[32,117,156],"on":[33,98,127,140,166,194,226],"binding":[35,124,251,276],"activity":[37,137,150,163,173,184,254,279],"acetylcholinesterase":[39],"(AChE)":[40],"following":[41],"direct":[42,267],"adsorption":[43,268],"to":[44,85,115,154,215,246],"modified":[45,100],"gold":[46,244],"surfaces.":[47,131,170],"modifications":[49],"included":[50],"self-assembled":[51],"monolayers":[52],"(SAMs)":[53],"terminated":[54,196],"\u2212COO":[56,199],"\u2013,":[57],"\u2212NH":[58,202],"3":[59,64,203,208],"+,":[60],"\u2212OH,":[61],"\u2212CH":[63,207],"functional":[65,209],"groups":[66],"at":[67,186],"varying":[68],"mole":[69],"%.":[70],"We":[71,107,132],"also":[72,133,233],"effects":[75],"positively":[77],"negatively":[79],"charged":[80],"helical":[81],"peptides":[82],"covalently":[83],"coupled":[84],"SAM.":[87],"Using":[88],"spectroscopic":[89],"ellipsometry,":[90],"measured":[92,134],"concentration":[95,111,176],"AChE":[97,139,188],"each":[99,141],"after":[102],"1":[103],"h":[104],"adsorption.":[106],"found":[108,148],"that":[109,149,269],"was":[112,125,151,164],"proportional":[114,153],"(":[118,157],"r":[119,158],"=":[120,159],"0.76).":[121],"The":[122,161],"highest":[123,162,183,236],"observed":[126,165,185],"more":[129,168],"hydrophobic":[130,220],"specific":[136,172],"using":[143],"colorimetric":[145],"assay":[146],"inversely":[152],"\u22120.71).":[160],"hydrophilic":[169,222],"Plotting":[171],"versus":[174,221],"showed":[177],"similar":[179,214],"relationship,":[180],"low":[187],"densities":[189],"(\u223c20%":[190],"monolayer)":[193],"surfaces":[195,232],"50%":[198,206],"\u2013":[200],"or":[201],"+":[204],"groups.":[210],"Interestingly,":[211],"is":[213],"approximate":[217],"composition":[218],"amino":[223],"acid":[224],"residues":[225],"AChE.":[230],"These":[231],"exhibited":[234],"total":[237],"activity:":[238],"\u223c100%":[240],"improvement":[241],"over":[242],"bare":[243],"due":[245],"combination":[248],"moderate":[250],"high":[253,275,278],"retention.":[255,280],"This":[256],"work":[257],"highlights":[258],"importance":[260],"developing":[262],"new":[263],"attachment":[264],"strategies":[265],"beyond":[266],"promote,":[270],"tune,":[271],"optimize":[273],"both":[274]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":4}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2023-07-14T00:00:00"}