{"id":"https://openalex.org/W4380894882","doi":"https://doi.org/10.55632/pwvas.v95i2.968","title":"Role of LpnA in Francisella tularensis Susceptibility to Resazurin.","display_name":"Role of LpnA in Francisella tularensis Susceptibility to Resazurin.","publication_year":2023,"publication_date":"2023-04-18","ids":{"openalex":"https://openalex.org/W4380894882","doi":"https://doi.org/10.55632/pwvas.v95i2.968"},"language":"en","primary_location":{"id":"doi:10.55632/pwvas.v95i2.968","doi":"https://doi.org/10.55632/pwvas.v95i2.968","is_oa":true,"landing_page_url":"http://dx.doi.org/10.55632/pwvas.v95i2.968","pdf_url":"https://pwvas.org/index.php/pwvas/article/download/968/915","source":{"id":"https://openalex.org/S4306524361","display_name":"Proceedings of the West Virginia Academy of Science","issn_l":"0096-4263","issn":["0096-4263","2473-0386"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the West Virginia Academy of Science","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://pwvas.org/index.php/pwvas/article/download/968/915","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5091484493","display_name":"Emma J. Beatty","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I31452180","display_name":"West Liberty University","ror":"https://ror.org/03gfrak98","country_code":"US","type":"education","lineage":["https://openalex.org/I31452180"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Emma Beatty","raw_affiliation_strings":["West Liberty University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"West Liberty University","institution_ids":["https://openalex.org/I31452180"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5024941923","display_name":"Deanna M. Schmitt","orcid":"https://orcid.org/0000-0001-6644-9124","observed_orcids":["https://orcid.org/0000-0001-6644-9124"]},"institutions":[{"id":"https://openalex.org/I31452180","display_name":"West Liberty University","ror":"https://ror.org/03gfrak98","country_code":"US","type":"education","lineage":["https://openalex.org/I31452180"]},{"id":"https://openalex.org/I88694374","display_name":"Marshall University","ror":"https://ror.org/02erqft81","country_code":"US","type":"education","lineage":["https://openalex.org/I88694374"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Deanna Schmitt","raw_affiliation_strings":["DONALD A. PRIMERANO , Department of Biomedical Sciences , Joan C. Edwards School of Medicine , Marshall University , Huntington , WV 25755 ","Department of Biomedical Sciences, West Liberty University, West Liberty, WV 26074, and"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"DONALD A. PRIMERANO , Department of Biomedical Sciences , Joan C. Edwards School of Medicine , Marshall University , Huntington , WV 25755 ","institution_ids":["https://openalex.org/I88694374"]},{"raw_affiliation_string":"Department of Biomedical Sciences, West Liberty University, West Liberty, WV 26074, and","institution_ids":["https://openalex.org/I31452180"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.06190468,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"95","issue":"2","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11515","display_name":"Bacillus and Francisella bacterial research","score":0.9887999892234802,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11515","display_name":"Bacillus and Francisella bacterial research","score":0.9887999892234802,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.0027000000700354576,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10147","display_name":"Antibiotic Resistance in Bacteria","score":0.0013000000035390258,"subfield":{"id":"https://openalex.org/subfields/1313","display_name":"Molecular Medicine"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/francisella-tularensis","display_name":"Francisella tularensis","score":0.9990000128746033},{"id":"https://openalex.org/keywords/resazurin","display_name":"resazurin","score":0.9779999852180481},{"id":"https://openalex.org/keywords/phenoxazine-dyes","display_name":"phenoxazine dyes","score":0.4830000102519989},{"id":"https://openalex.org/keywords/antibiotic-resistance","display_name":"antibiotic resistance","score":0.7599999904632568},{"id":"https://openalex.org/keywords/antimicrobial-susceptibility","display_name":"antimicrobial susceptibility","score":0.7009999752044678},{"id":"https://openalex.org/keywords/complementation","display_name":"complementation","score":0.5619999766349792},{"id":"https://openalex.org/keywords/katg","display_name":"katG","score":0.7630000114440918},{"id":"https://openalex.org/keywords/tularemia","display_name":"tularemia","score":0.7549999952316284},{"id":"https://openalex.org/keywords/bioterrorism-agents","display_name":"bioterrorism agents","score":0.8529999852180481},{"id":"https://openalex.org/keywords/minimum-inhibitory-concentration","display_name":"minimum inhibitory concentration","score":0.671999990940094}],"concepts":[{"id":"https://openalex.org/C2780467176","wikidata":"https://www.wikidata.org/wiki/Q1003460","display_name":"Francisella tularensis","level":4,"score":0.8786895871162415},{"id":"https://openalex.org/C2775983634","wikidata":"https://www.wikidata.org/wiki/Q153861","display_name":"Tularemia","level":2,"score":0.8694287538528442},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.6249895691871643},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6184118390083313},{"id":"https://openalex.org/C2778400546","wikidata":"https://www.wikidata.org/wiki/Q409628","display_name":"Resazurin","level":2,"score":0.5691009163856506},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.4872668981552124},{"id":"https://openalex.org/C94665300","wikidata":"https://www.wikidata.org/wiki/Q63391344","display_name":"Antibiotic resistance","level":3,"score":0.4184877872467041},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.3685299754142761},{"id":"https://openalex.org/C501593827","wikidata":"https://www.wikidata.org/wiki/Q12187","display_name":"Antibiotics","level":2,"score":0.3222680687904358},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2878437042236328},{"id":"https://openalex.org/C60987743","wikidata":"https://www.wikidata.org/wiki/Q1460232","display_name":"Virulence","level":3,"score":0.20625194907188416},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.14375483989715576}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.55632/pwvas.v95i2.968","doi":"https://doi.org/10.55632/pwvas.v95i2.968","is_oa":true,"landing_page_url":"http://dx.doi.org/10.55632/pwvas.v95i2.968","pdf_url":"https://pwvas.org/index.php/pwvas/article/download/968/915","source":{"id":"https://openalex.org/S4306524361","display_name":"Proceedings of the West Virginia Academy of Science","issn_l":"0096-4263","issn":["0096-4263","2473-0386"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the West Virginia Academy of Science","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.55632/pwvas.v95i2.968","doi":"https://doi.org/10.55632/pwvas.v95i2.968","is_oa":true,"landing_page_url":"http://dx.doi.org/10.55632/pwvas.v95i2.968","pdf_url":"https://pwvas.org/index.php/pwvas/article/download/968/915","source":{"id":"https://openalex.org/S4306524361","display_name":"Proceedings of the West Virginia Academy of Science","issn_l":"0096-4263","issn":["0096-4263","2473-0386"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the West Virginia Academy of Science","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.7049999833106995,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4380894882.pdf","grobid_xml":"https://content.openalex.org/works/W4380894882.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4380894937","https://openalex.org/W4285687361","https://openalex.org/W4286822615","https://openalex.org/W3172029489","https://openalex.org/W4285687219","https://openalex.org/W4280649635","https://openalex.org/W3194148209","https://openalex.org/W4380894879","https://openalex.org/W4285687297","https://openalex.org/W2607314312"],"abstract_inverted_index":{"Francisella":[0],"tularensis":[1,51,76],"is":[2,33,54,88],"defined":[3],"by":[4,244],"the":[5,39,43,66,109,117,149,152,180,201,205,226,237],"Centers":[6],"for":[7,58,103],"Disease":[8],"Control":[9],"and":[10,27,42,46,77,124,146,164],"Prevention":[11],"as":[12],"a":[13,99,139,246],"Category":[14],"A":[15],"bioterrorism":[16],"agent":[17],"due":[18,199],"to":[19,134,171,200,230,235],"its":[20],"low":[21],"infectious":[22],"dose,":[23],"high":[24,100],"mortality":[25],"rate,":[26],"ease":[28],"of":[29,48,83,85,94,108,120,130,142,151,162,177,217,239],"aerosolization.":[30],"Given":[31],"there":[32,53],"no":[34],"licensed":[35],"tularemia":[36],"vaccine":[37],"in":[38,98,179,192,197,241],"United":[40],"States":[41],"possible":[44],"development":[45],"release":[47],"antibiotic-resistant":[49],"F.":[50,75],"strains,":[52],"an":[55,232],"urgent":[56],"need":[57],"new":[59],"treatments":[60],"against":[61,74],"this":[62,86],"bacterium.":[63],"We":[64],"determined":[65],"phenoxazine":[67],"dye":[68],"resazurin":[69,242],"(Rz)":[70],"exhibits":[71],"antimicrobial":[72],"activity":[73],"other":[78],"gram-negative":[79],"bacteria.":[80],"The":[81,157],"mode":[82],"action":[84],"compound":[87],"not":[89,196,214],"understood,":[90],"but":[91,195],"potential":[92],"targets":[93],"Rz":[95,135],"were":[96,166,174],"identified":[97],"throughput":[101],"screen":[102],"resistant":[104],"isolates.":[105],"Ninety-three":[106],"percent":[107],"Rz-resistant":[110],"(Rzr)":[111],"isolates":[112],"sequenced":[113],"contained":[114],"mutations":[115],"within":[116],"coding":[118],"regions":[119],"FTL_0421":[121],"(lpnA),":[122],"FTL_0895,":[123],"FTL_1504":[125],"(katG).":[126],"To":[127],"confirm":[128,172],"mutation":[129],"lpnA":[131,143,247],"was":[132,190,223],"contributing":[133],"resistance,":[136],"we":[137,169,173,211,228],"introduced":[138],"wild-type":[140,193],"copy":[141],"into":[144],"Rzr1":[145,163,198,206],"then":[147],"assessed":[148],"susceptibility":[150],"resulting":[153],"complemented":[154,181],"strain":[155,182,207],"(Rzr1/pABST-lpnA).":[156],"minimum":[158],"inhibitory":[159],"concentration":[160],"(MIC)":[161],"Rzr1/pABST-lpnA":[165],"similar,":[167],"therefore,":[168],"wanted":[170],"restoring":[175],"expression":[176,216],"LpnA":[178,189,218,240],"via":[183],"Western":[184],"blot":[185],"analysis.":[186],"As":[187],"expected,":[188],"expressed":[191],"LVS":[194],"genetic":[202],"mutation.":[203],"In":[204,225],"transformed":[208],"with":[209],"pABST-lpnA,":[210],"still":[212],"did":[213],"observe":[215],"suggesting":[219],"our":[220],"complementation":[221],"strategy":[222],"ineffective.":[224],"future,":[227],"plan":[229],"take":[231],"alternative":[233],"approach":[234],"investigate":[236],"role":[238],"resistance":[243],"creating":[245],"deletion":[248],"mutant.":[249]},"counts_by_year":[],"updated_date":"2026-10-30T12:39:25.321706","created_date":"2023-06-17T00:00:00"}