{"id":"https://openalex.org/W4380080845","doi":"https://doi.org/10.3389/fpls.2023.1196618","title":"A method for analyzing programmed cell death in xylem development by flow cytometry","display_name":"A method for analyzing programmed cell death in xylem development by flow cytometry","publication_year":2023,"publication_date":"2023-06-09","ids":{"openalex":"https://openalex.org/W4380080845","doi":"https://doi.org/10.3389/fpls.2023.1196618","pmid":"https://pubmed.ncbi.nlm.nih.gov/37360718","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/10288846"},"language":"en","primary_location":{"id":"doi:10.3389/fpls.2023.1196618","doi":"https://doi.org/10.3389/fpls.2023.1196618","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fpls.2023.1196618","pdf_url":"https://public-pages-files-2025.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1196618/pdf","source":{"id":"https://openalex.org/S2493613807","display_name":"Frontiers in Plant Science","issn_l":"1664-462X","issn":["1664-462X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Plant Science","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","datacite","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://public-pages-files-2025.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1196618/pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100643733","display_name":"Yingli Liu","orcid":"https://orcid.org/0000-0001-8464-7917","observed_orcids":["https://orcid.org/0000-0001-8464-7917"]},"institutions":[{"id":"https://openalex.org/I4210128615","display_name":"Chinese Academy of Forestry","ror":"https://ror.org/0360dkv71","country_code":"CN","type":"government","lineage":["https://openalex.org/I4210128615","https://openalex.org/I4210134523"]},{"id":"https://openalex.org/I4392021092","display_name":"State Key Laboratory of Tree Genetics and Breeding","ror":"https://ror.org/03a1zvg88","country_code":"CN","type":"facility","lineage":["https://openalex.org/I4210128615","https://openalex.org/I4210134523","https://openalex.org/I4392021092","https://openalex.org/I47689461"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Ying-Li Liu","raw_affiliation_strings":["State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, China","institution_ids":["https://openalex.org/I4210128615","https://openalex.org/I4392021092"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5023714026","display_name":"Yinghua Guo","orcid":"https://orcid.org/0009-0008-1724-3603","observed_orcids":["https://orcid.org/0009-0008-1724-3603"]},"institutions":[{"id":"https://openalex.org/I20231570","display_name":"Peking University","ror":"https://ror.org/02v51f717","country_code":"CN","type":"education","lineage":["https://openalex.org/I20231570"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Ying-Hua Guo","raw_affiliation_strings":["National Center for Protein Sciences at Peking University, Beijing, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Center for Protein Sciences at Peking University, Beijing, China","institution_ids":["https://openalex.org/I20231570"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5019569208","display_name":"Xueqin Song","orcid":"https://orcid.org/0000-0001-8938-3551","observed_orcids":["https://orcid.org/0000-0001-8938-3551"]},"institutions":[{"id":"https://openalex.org/I4210128615","display_name":"Chinese Academy of Forestry","ror":"https://ror.org/0360dkv71","country_code":"CN","type":"government","lineage":["https://openalex.org/I4210128615","https://openalex.org/I4210134523"]},{"id":"https://openalex.org/I4210132245","display_name":"Research Institute of Forestry","ror":"https://ror.org/02nmvgz47","country_code":"CN","type":"facility","lineage":["https://openalex.org/I4210128615","https://openalex.org/I4210132245","https://openalex.org/I4210134523"]},{"id":"https://openalex.org/I4392021092","display_name":"State Key Laboratory of Tree Genetics and Breeding","ror":"https://ror.org/03a1zvg88","country_code":"CN","type":"facility","lineage":["https://openalex.org/I4210128615","https://openalex.org/I4210134523","https://openalex.org/I4392021092","https://openalex.org/I47689461"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xue-Qin Song","raw_affiliation_strings":["State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China","institution_ids":["https://openalex.org/I4210128615","https://openalex.org/I4210132245","https://openalex.org/I4392021092"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5052242799","display_name":"Meng\u2010Xuan Hu","orcid":"https://orcid.org/0000-0001-5530-4181","observed_orcids":["https://orcid.org/0000-0001-5530-4181"]},"institutions":[{"id":"https://openalex.org/I4210128615","display_name":"Chinese Academy of Forestry","ror":"https://ror.org/0360dkv71","country_code":"CN","type":"government","lineage":["https://openalex.org/I4210128615","https://openalex.org/I4210134523"]},{"id":"https://openalex.org/I4210132245","display_name":"Research Institute of Forestry","ror":"https://ror.org/02nmvgz47","country_code":"CN","type":"facility","lineage":["https://openalex.org/I4210128615","https://openalex.org/I4210132245","https://openalex.org/I4210134523"]},{"id":"https://openalex.org/I4392021092","display_name":"State Key Laboratory of Tree Genetics and Breeding","ror":"https://ror.org/03a1zvg88","country_code":"CN","type":"facility","lineage":["https://openalex.org/I4210128615","https://openalex.org/I4210134523","https://openalex.org/I4392021092","https://openalex.org/I47689461"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Meng-Xuan Hu","raw_affiliation_strings":["State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China","institution_ids":["https://openalex.org/I4210128615","https://openalex.org/I4210132245","https://openalex.org/I4392021092"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5062330687","display_name":"Shutang Zhao","orcid":"https://orcid.org/0000-0002-8818-5841","observed_orcids":["https://orcid.org/0000-0002-8818-5841"]},"institutions":[{"id":"https://openalex.org/I4210128615","display_name":"Chinese Academy of Forestry","ror":"https://ror.org/0360dkv71","country_code":"CN","type":"government","lineage":["https://openalex.org/I4210128615","https://openalex.org/I4210134523"]},{"id":"https://openalex.org/I4210132245","display_name":"Research Institute of Forestry","ror":"https://ror.org/02nmvgz47","country_code":"CN","type":"facility","lineage":["https://openalex.org/I4210128615","https://openalex.org/I4210132245","https://openalex.org/I4210134523"]},{"id":"https://openalex.org/I4392021092","display_name":"State Key Laboratory of Tree Genetics and Breeding","ror":"https://ror.org/03a1zvg88","country_code":"CN","type":"facility","lineage":["https://openalex.org/I4210128615","https://openalex.org/I4210134523","https://openalex.org/I4392021092","https://openalex.org/I47689461"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Shu-Tang Zhao","raw_affiliation_strings":["State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China","institution_ids":["https://openalex.org/I4210128615","https://openalex.org/I4210132245","https://openalex.org/I4392021092"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5062330687"],"corresponding_institution_ids":["https://openalex.org/I4210128615","https://openalex.org/I4210132245","https://openalex.org/I4392021092"],"apc_list":{"value":3295,"currency":"USD","value_usd":3295},"apc_paid":{"value":3295,"currency":"USD","value_usd":3295},"fwci":0.7555,"has_fulltext":true,"cited_by_count":10,"citation_normalized_percentile":{"value":0.63045622,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"14","issue":null,"first_page":"1196618","last_page":"1196618"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10294","display_name":"Cell death mechanisms and regulation","score":0.725600004196167,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10294","display_name":"Cell death mechanisms and regulation","score":0.725600004196167,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10184","display_name":"Plant Molecular Biology Research","score":0.13899999856948853,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10869","display_name":"Polysaccharides and Plant Cell Walls","score":0.02810000069439411,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/programmed-cell-death","display_name":"programmed cell death","score":0.9990000128746033},{"id":"https://openalex.org/keywords/wood-formation","display_name":"wood formation","score":0.9919999837875366},{"id":"https://openalex.org/keywords/poplar","display_name":"poplar","score":0.7839999794960022},{"id":"https://openalex.org/keywords/annexin-v-fitc","display_name":"annexin V-FITC","score":0.8309999704360962},{"id":"https://openalex.org/keywords/propidium-iodide","display_name":"propidium iodide","score":0.9760000109672546},{"id":"https://openalex.org/keywords/fluorescence-activated-cell-sorting","display_name":"fluorescence-activated cell sorting","score":0.8519999980926514},{"id":"https://openalex.org/keywords/cell-death-detection","display_name":"cell death detection","score":0.4429999887943268},{"id":"https://openalex.org/keywords/marker-gene-expression","display_name":"marker gene expression","score":0.7839999794960022},{"id":"https://openalex.org/keywords/woody-plants","display_name":"woody plants","score":0.8980000019073486}],"concepts":[{"id":"https://openalex.org/C2775934118","wikidata":"https://www.wikidata.org/wiki/Q424169","display_name":"Propidium iodide","level":4,"score":0.8488531112670898},{"id":"https://openalex.org/C88634738","wikidata":"https://www.wikidata.org/wiki/Q24769543","display_name":"Annexin","level":3,"score":0.8016363382339478},{"id":"https://openalex.org/C553184892","wikidata":"https://www.wikidata.org/wiki/Q1141429","display_name":"Flow cytometry","level":2,"score":0.7743791937828064},{"id":"https://openalex.org/C31573885","wikidata":"https://www.wikidata.org/wiki/Q304484","display_name":"Programmed cell death","level":3,"score":0.7702874541282654},{"id":"https://openalex.org/C2778630268","wikidata":"https://www.wikidata.org/wiki/Q17113277","display_name":"Cell sorting","level":3,"score":0.6785100698471069},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.6319652199745178},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6217818260192871},{"id":"https://openalex.org/C190283241","wikidata":"https://www.wikidata.org/wiki/Q14599311","display_name":"Apoptosis","level":2,"score":0.6014125347137451},{"id":"https://openalex.org/C49799701","wikidata":"https://www.wikidata.org/wiki/Q122811","display_name":"Xylem","level":2,"score":0.4681057631969452},{"id":"https://openalex.org/C156203801","wikidata":"https://www.wikidata.org/wiki/Q1056194","display_name":"Plant cell","level":3,"score":0.4579886198043823},{"id":"https://openalex.org/C53009064","wikidata":"https://www.wikidata.org/wiki/Q167","display_name":"Pi","level":2,"score":0.45577478408813477},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.3975893557071686},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.2982356548309326},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.18859481811523438},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.1309487223625183}],"mesh":[],"locations_count":6,"locations":[{"id":"doi:10.3389/fpls.2023.1196618","doi":"https://doi.org/10.3389/fpls.2023.1196618","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fpls.2023.1196618","pdf_url":"https://public-pages-files-2025.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1196618/pdf","source":{"id":"https://openalex.org/S2493613807","display_name":"Frontiers in Plant Science","issn_l":"1664-462X","issn":["1664-462X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Plant Science","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:10288846","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/10288846","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC10288846/pdf/fpls-14-1196618.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Front Plant Sci","raw_type":"Text"},{"id":"pmid:37360718","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/37360718","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in plant science","raw_type":"Journal Article"},{"id":"pmh:oai:doaj.org/article:f06795603a984f928e6649e958d06304","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/f06795603a984f928e6649e958d06304","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Frontiers in Plant Science, Vol 14 (2023)","raw_type":"article"},{"id":"doi:10.60692/3a4a4-nvg15","doi":"https://doi.org/10.60692/3a4a4-nvg15","is_oa":true,"landing_page_url":"https://doi.org/10.60692/3a4a4-nvg15","pdf_url":null,"source":{"id":"https://openalex.org/S7407051682","display_name":"Greater South Information System","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"article-journal"},{"id":"doi:10.60692/f90v1-ct941","doi":"https://doi.org/10.60692/f90v1-ct941","is_oa":true,"landing_page_url":"https://doi.org/10.60692/f90v1-ct941","pdf_url":null,"source":{"id":"https://openalex.org/S7407051682","display_name":"Greater South Information System","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"article-journal"}],"best_oa_location":{"id":"doi:10.3389/fpls.2023.1196618","doi":"https://doi.org/10.3389/fpls.2023.1196618","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fpls.2023.1196618","pdf_url":"https://public-pages-files-2025.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1196618/pdf","source":{"id":"https://openalex.org/S2493613807","display_name":"Frontiers in Plant Science","issn_l":"1664-462X","issn":["1664-462X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Plant Science","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G3587219304","display_name":null,"funder_award_id":"2019YFE0119100","funder_id":"https://openalex.org/F4320335777","funder_display_name":"National Key Research and Development Program of China"}],"funders":[{"id":"https://openalex.org/F4320324787","display_name":"Peking University","ror":"https://ror.org/02v51f717"},{"id":"https://openalex.org/F4320335777","display_name":"National Key Research and Development Program of China","ror":null}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4380080845.pdf","grobid_xml":"https://content.openalex.org/works/W4380080845.grobid-xml"},"referenced_works_count":38,"referenced_works":["https://openalex.org/W267664099","https://openalex.org/W1590434810","https://openalex.org/W1831137141","https://openalex.org/W1965749819","https://openalex.org/W1971802247","https://openalex.org/W1979129905","https://openalex.org/W1984620151","https://openalex.org/W1985221982","https://openalex.org/W2002318669","https://openalex.org/W2036712556","https://openalex.org/W2038038128","https://openalex.org/W2063346783","https://openalex.org/W2065358406","https://openalex.org/W2085222580","https://openalex.org/W2106316095","https://openalex.org/W2109396083","https://openalex.org/W2138826821","https://openalex.org/W2144338404","https://openalex.org/W2155796246","https://openalex.org/W2466579921","https://openalex.org/W2577128452","https://openalex.org/W2884964182","https://openalex.org/W2884987394","https://openalex.org/W2888121038","https://openalex.org/W3044145779","https://openalex.org/W3109691794","https://openalex.org/W3118684161","https://openalex.org/W3164828006","https://openalex.org/W3168747467","https://openalex.org/W3188661367","https://openalex.org/W3193018801","https://openalex.org/W3198167618","https://openalex.org/W3215442425","https://openalex.org/W3216870676","https://openalex.org/W4292790997","https://openalex.org/W4293582256","https://openalex.org/W4313893361","https://openalex.org/W4323664587"],"related_works":["https://openalex.org/W7620048","https://openalex.org/W2387575411","https://openalex.org/W1169884202","https://openalex.org/W2389832506","https://openalex.org/W2383041652","https://openalex.org/W7096289580","https://openalex.org/W2885466509","https://openalex.org/W2599315385","https://openalex.org/W7164160403","https://openalex.org/W7165923979"],"abstract_inverted_index":{"Programmed":[0],"cell":[1,78,151,159],"death":[2,13],"(PCD)":[3],"is":[4,49,60],"a":[5,86],"genetically":[6],"regulated":[7],"developmental":[8,143],"process":[9],"leading":[10],"to":[11,38,41,52,63,166],"the":[12,76,150,155,178],"of":[14,17,88,140,181],"specific":[15],"types":[16],"plant":[18,25],"cells,":[19,57],"which":[20,172],"plays":[21],"important":[22],"roles":[23],"in":[24,44,55,66,69,169],"development":[26],"and":[27,92,96,117,132,142],"growth":[28],"such":[29],"as":[30],"wood":[31,182],"formation.":[32,183],"However,":[33],"an":[34],"efficient":[35],"method":[36,131,162],"needs":[37],"be":[39,126,164,174],"established":[40],"study":[42,167],"PCD":[43,65,111,119,168],"woody":[45,70,170],"plants.":[46,71],"Flow":[47],"cytometry":[48],"widely":[50],"utilized":[51],"evaluate":[53],"apoptosis":[54],"mammalian":[56],"while":[58],"it":[59],"rarely":[61],"used":[62,165],"detect":[64],"plants,":[67,171],"especially":[68],"Here,":[72],"we":[73],"reported":[74],"that":[75],"xylem":[77],"protoplasts":[79],"from":[80],"poplar":[81],"stem":[82],"were":[83],"stained":[84],"with":[85,149],"combination":[87],"fluorescein":[89],"annexin":[90],"V-FITC":[91,107,114,122],"propidium":[93],"iodide":[94],"(PI)":[95],"then":[97,133],"sorted":[98],"by":[99],"flow":[100],"cytometry.":[101],"As":[102],"expected,":[103],"living":[104],"cells":[105,112,120],"(annexin":[106,113,121],"negative/PI":[108],"negative),":[109,116],"early":[110],"positive/PI":[115,123],"late":[118],"positive)":[124],"could":[125],"finely":[127],"distinguished":[128],"through":[129],"this":[130],"subjected":[134],"for":[135,176],"quantitative":[136],"analysis.":[137],"The":[138],"expression":[139],"cell-type-":[141],"stages-specific":[144],"marker":[145],"genes":[146],"was":[147],"consistent":[148],"morphological":[152],"observation.":[153],"Therefore,":[154],"newly":[156],"developed":[157],"fluorescence-activated":[158],"sorting":[160],"(FACS)":[161],"can":[163],"will":[173],"beneficial":[175],"studying":[177],"molecular":[179],"mechanisms":[180]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":4},{"year":2024,"cited_by_count":4},{"year":2023,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2023-06-10T00:00:00"}