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THE BINDING SITES OF THE DIATOMIC SUBSTRATE AND PRODUCT AS WELL AS THE SOURCE OF THE PROTONS IN THE REACTION HAVE BEEN STUDIED  BUT THEIR EXACT IDENTIFICATION HAS NOT BEEN POSSIBLE. THIS DETAILED INFORMATION CAN ONLY BE DETERMINED BY NEUTRON DIFFRACTION. COMPLEXES OF HUMAN MNSOD INCLUDING STRUCTURAL INTERMEDIATES AND MUTANTS WILL BE THE TARGETS FOR LARGE VOLUME CRYSTAL ( 1MM3) GROWTH FOR STRUCTURE DETERMINATION BY NEUTRON MACROMOLECULAR CRYSTALLOGRAPHY (NMC). THE QUIESCENT ENVIRONMENT AFFORDED BY MICROGRAVITY IS KNOWN TO GROW CRYSTALS LARGE ENOUGH FOR NEUTRON STUDIES NOT ONLY ARE THEY LARGE BUT THEIR QUALITY APPROACHES PERFECTION. IN 2001  THE BORGSTAHL LABORATORY SUCCESSFULLY GREW LARGE CRYSTALS OF SOD USING MICROGRAVITY CONDITIONS ON THE INTERNATIONAL SPACE STATION (ISS). WITH NASA S RENEWED INTEREST IN IMPLEMENTING THE MICROGRAVITY ENVIRONMENT ON THE ISS FOR PROTEIN CRYSTAL GROWTH WE WOULD LIKE TO MOVE FORWARD WITH THESE EXCITING EARLY MICROGRAVITY CRYSTALLIZATION RESULTS FOR SOD. EXISTING CRYSTALLIZATION HARDWARE THAT USES THE GRANADA CRYSTALLIZATION BOXES (GCB) FOR CAPILLARY COUNTERDIFFUSION PROTOCOLS WILL BE USED TO ACHIEVE THESE GOALS. A MICROGRAVITY ENVIRONMENT IS ESSENTIAL TO FORM A STABLE SUPERSATURATION GRADIENT TO OBTAIN THE LARGE  HIGH QUALITY CRYSTALS REQUIRED FOR NMC. THEN NMC WILL BE PERFORMED WITH COLLABORATORS AT OAK RIDGE NATIONAL LABORATORY (ORNL). THE PRINCIPAL OUTCOME WILL BE TO IDENTIFY THE ROLE OF HYDROGEN ATOMS IN ENZYMATIC ACTIVITY  DISCERN SUPEROXIDE FROM PEROXIDE  AND WATER FROM HYDROXIDE ION BY THEIR PROTONATION STATE AND DECIPHER A STRUCTURE-BASED MECHANISM FOR HUMAN MNSOD MORE PRECISELY THAN FROM PREVIOUS X-RAY CRYSTALLOGRAPHIC MODELS DETERMINED FROM EARTH-GROWN CRYSTALS. 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