{"id":"https://openalex.org/W4376573128","doi":"https://doi.org/10.1039/d3sm00219e","title":"Viscoelasticity of single folded proteins using dynamic atomic force microscopy","display_name":"Viscoelasticity of single folded proteins using dynamic atomic force microscopy","publication_year":2023,"publication_date":"2023-01-01","ids":{"openalex":"https://openalex.org/W4376573128","doi":"https://doi.org/10.1039/d3sm00219e","pmid":"https://pubmed.ncbi.nlm.nih.gov/37266565"},"language":"en","primary_location":{"id":"doi:10.1039/d3sm00219e","doi":"https://doi.org/10.1039/d3sm00219e","is_oa":false,"landing_page_url":"https://doi.org/10.1039/d3sm00219e","pdf_url":null,"source":{"id":"https://openalex.org/S6377669","display_name":"Soft Matter","issn_l":"1744-683X","issn":["1744-683X","1744-6848"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","jufo-2","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320556","host_organization_name":"Royal Society of Chemistry","host_organization_lineage":["https://openalex.org/P4310320556"],"host_organization_lineage_names":["Royal Society of Chemistry"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Soft Matter","raw_type":"journal-article"},"volume":null,"type":"review","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5060650695","display_name":"Surya Pratap S Deopa","orcid":"https://orcid.org/0000-0003-1173-8011","observed_orcids":["https://orcid.org/0000-0003-1173-8011"]},"institutions":[{"id":"https://openalex.org/I181996519","display_name":"Indian Institute of Science Education and Research Pune","ror":"https://ror.org/028qa3n13","country_code":"IN","type":"education","lineage":["https://openalex.org/I181996519"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Surya Pratap S. Deopa","raw_affiliation_strings":["Department of Physics, Indian Institute of Science Education and Research, Pune, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Physics, Indian Institute of Science Education and Research, Pune, India","institution_ids":["https://openalex.org/I181996519"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5047309255","display_name":"Shivprasad Patil","orcid":"https://orcid.org/0000-0002-3413-4109","observed_orcids":["https://orcid.org/0000-0002-3413-4109"]},"institutions":[{"id":"https://openalex.org/I181996519","display_name":"Indian Institute of Science Education and Research Pune","ror":"https://ror.org/028qa3n13","country_code":"IN","type":"education","lineage":["https://openalex.org/I181996519"]}],"countries":["IN"],"is_corresponding":true,"raw_author_name":"Shivprasad Patil","raw_affiliation_strings":["Department of Physics, Indian Institute of Science Education and Research, Pune, India"],"raw_orcid":"https://orcid.org/0000-0002-3413-4109","affiliations":[{"raw_affiliation_string":"Department of Physics, Indian Institute of Science Education and Research, Pune, India","institution_ids":["https://openalex.org/I181996519"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5047309255"],"corresponding_institution_ids":["https://openalex.org/I181996519"],"apc_list":null,"apc_paid":null,"fwci":0.059,"has_fulltext":false,"cited_by_count":3,"citation_normalized_percentile":{"value":0.0390625,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"19","issue":"23","first_page":"4188","last_page":"4203"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10923","display_name":"Force Microscopy Techniques and Applications","score":0.9940999746322632,"subfield":{"id":"https://openalex.org/subfields/3107","display_name":"Atomic and Molecular Physics, and Optics"},"field":{"id":"https://openalex.org/fields/31","display_name":"Physics and Astronomy"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10923","display_name":"Force Microscopy Techniques and Applications","score":0.9940999746322632,"subfield":{"id":"https://openalex.org/subfields/3107","display_name":"Atomic and Molecular Physics, and Optics"},"field":{"id":"https://openalex.org/fields/31","display_name":"Physics and Astronomy"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.00570000009611249,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10379","display_name":"Cellular Mechanics and Interactions","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dynamic-atomic-force-microscopy","display_name":"dynamic force microscopy","score":0.9419999718666077},{"id":"https://openalex.org/keywords/single-molecule-force-spectroscopy","display_name":"single-molecule force spectroscopy","score":0.8050000071525574},{"id":"https://openalex.org/keywords/phase-lag","display_name":"phase lag","score":0.9210000038146973},{"id":"https://openalex.org/keywords/protein-unfolding","display_name":"protein unfolding","score":0.5590000152587891},{"id":"https://openalex.org/keywords/protein-dynamics","display_name":"protein dynamics","score":0.5690000057220459},{"id":"https://openalex.org/keywords/structural-flexibility","display_name":"structural flexibility","score":0.9010000228881836},{"id":"https://openalex.org/keywords/measurement-artifacts","display_name":"measurement artifacts","score":0.6869999766349792}],"concepts":[{"id":"https://openalex.org/C141354745","wikidata":"https://www.wikidata.org/wiki/Q17227","display_name":"Cantilever","level":2,"score":0.8535112738609314},{"id":"https://openalex.org/C186541917","wikidata":"https://www.wikidata.org/wiki/Q910483","display_name":"Viscoelasticity","level":2,"score":0.8447598814964294},{"id":"https://openalex.org/C102951782","wikidata":"https://www.wikidata.org/wiki/Q49295","display_name":"Atomic force microscopy","level":2,"score":0.7257775068283081},{"id":"https://openalex.org/C199723944","wikidata":"https://www.wikidata.org/wiki/Q3041998","display_name":"Force dynamics","level":2,"score":0.6555341482162476},{"id":"https://openalex.org/C71246147","wikidata":"https://www.wikidata.org/wiki/Q16029538","display_name":"Non-contact atomic force microscopy","level":4,"score":0.5696085691452026},{"id":"https://openalex.org/C180205008","wikidata":"https://www.wikidata.org/wiki/Q159190","display_name":"Amplitude","level":2,"score":0.5244812369346619},{"id":"https://openalex.org/C147080431","wikidata":"https://www.wikidata.org/wiki/Q1074953","display_name":"Microscopy","level":2,"score":0.5084397196769714},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.48761311173439026},{"id":"https://openalex.org/C44280652","wikidata":"https://www.wikidata.org/wiki/Q104837","display_name":"Phase (matter)","level":2,"score":0.43339288234710693},{"id":"https://openalex.org/C43826995","wikidata":"https://www.wikidata.org/wiki/Q17014103","display_name":"Atomic force acoustic microscopy","level":5,"score":0.41155216097831726},{"id":"https://openalex.org/C206008964","wikidata":"https://www.wikidata.org/wiki/Q5159384","display_name":"Conductive atomic force microscopy","level":3,"score":0.3563145399093628},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3434023857116699},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.3325514793395996},{"id":"https://openalex.org/C41999313","wikidata":"https://www.wikidata.org/wiki/Q489328","display_name":"Molecular physics","level":1,"score":0.33196327090263367},{"id":"https://openalex.org/C159467904","wikidata":"https://www.wikidata.org/wiki/Q2001702","display_name":"Chemical physics","level":1,"score":0.3288452923297882},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.2887952923774719},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.26863330602645874},{"id":"https://openalex.org/C181635281","wikidata":"https://www.wikidata.org/wiki/Q2799395","display_name":"Magnetic force microscope","level":4,"score":0.2568609416484833},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.18705302476882935},{"id":"https://openalex.org/C159985019","wikidata":"https://www.wikidata.org/wiki/Q181790","display_name":"Composite material","level":1,"score":0.16883832216262817},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.08769693970680237},{"id":"https://openalex.org/C78519656","wikidata":"https://www.wikidata.org/wiki/Q101333","display_name":"Mechanical engineering","level":1,"score":0.0680837333202362},{"id":"https://openalex.org/C32546565","wikidata":"https://www.wikidata.org/wiki/Q856711","display_name":"Magnetization","level":3,"score":0.0},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C115260700","wikidata":"https://www.wikidata.org/wiki/Q11408","display_name":"Magnetic field","level":2,"score":0.0},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D018625","descriptor_name":"Microscopy, Atomic Force","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D055595","descriptor_name":"Mechanical Phenomena","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1039/d3sm00219e","doi":"https://doi.org/10.1039/d3sm00219e","is_oa":false,"landing_page_url":"https://doi.org/10.1039/d3sm00219e","pdf_url":null,"source":{"id":"https://openalex.org/S6377669","display_name":"Soft Matter","issn_l":"1744-683X","issn":["1744-683X","1744-6848"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","jufo-2","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320556","host_organization_name":"Royal Society of Chemistry","host_organization_lineage":["https://openalex.org/P4310320556"],"host_organization_lineage_names":["Royal Society of Chemistry"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Soft Matter","raw_type":"journal-article"},{"id":"pmid:37266565","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/37266565","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Soft matter","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G4560392682","display_name":null,"funder_award_id":"CRG/2022/001891","funder_id":"https://openalex.org/F4320334771","funder_display_name":"Science and Engineering Research Board"}],"funders":[{"id":"https://openalex.org/F4320334771","display_name":"Science and Engineering Research Board","ror":"https://ror.org/03ffdsr55"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":184,"referenced_works":["https://openalex.org/W1505577908","https://openalex.org/W1516602187","https://openalex.org/W1572609533","https://openalex.org/W1963734125","https://openalex.org/W1966188810","https://openalex.org/W1968498274","https://openalex.org/W1968734496","https://openalex.org/W1969262554","https://openalex.org/W1970265754","https://openalex.org/W1970633951","https://openalex.org/W1973943659","https://openalex.org/W1974004204","https://openalex.org/W1974262428","https://openalex.org/W1974310050","https://openalex.org/W1978633697","https://openalex.org/W1978696831","https://openalex.org/W1978932874","https://openalex.org/W1979018519","https://openalex.org/W1979863594","https://openalex.org/W1980745639","https://openalex.org/W1981716245","https://openalex.org/W1983362126","https://openalex.org/W1986037089","https://openalex.org/W1986916659","https://openalex.org/W1987374645","https://openalex.org/W1992984362","https://openalex.org/W1994069050","https://openalex.org/W1995379884","https://openalex.org/W1996333756","https://openalex.org/W1998196091","https://openalex.org/W2001584038","https://openalex.org/W2003252350","https://openalex.org/W2003285395","https://openalex.org/W2003761934","https://openalex.org/W2004522688","https://openalex.org/W2004523512","https://openalex.org/W2006070959","https://openalex.org/W2007422975","https://openalex.org/W2008718474","https://openalex.org/W2010227280","https://openalex.org/W2011385542","https://openalex.org/W2013151264","https://openalex.org/W2013344079","https://openalex.org/W2013581614","https://openalex.org/W2013906676","https://openalex.org/W2014852739","https://openalex.org/W2015134782","https://openalex.org/W2015667791","https://openalex.org/W2019738822","https://openalex.org/W2019877102","https://openalex.org/W2022598898","https://openalex.org/W2023401067","https://openalex.org/W2025451503","https://openalex.org/W2029137764","https://openalex.org/W2033391625","https://openalex.org/W2033774200","https://openalex.org/W2035469922","https://openalex.org/W2036957419","https://openalex.org/W2040428768","https://openalex.org/W2042205161","https://openalex.org/W2044451368","https://openalex.org/W2044646146","https://openalex.org/W2046076659","https://openalex.org/W2047397572","https://openalex.org/W2048555993","https://openalex.org/W2048877007","https://openalex.org/W2049422806","https://openalex.org/W2049888624","https://openalex.org/W2050017497","https://openalex.org/W2050946447","https://openalex.org/W2051001465","https://openalex.org/W2051675607","https://openalex.org/W2052652876","https://openalex.org/W2053837249","https://openalex.org/W2057889396","https://openalex.org/W2058322086","https://openalex.org/W2058912578","https://openalex.org/W2058983530","https://openalex.org/W2065281489","https://openalex.org/W2065907078","https://openalex.org/W2066385645","https://openalex.org/W2066619919","https://openalex.org/W2066801946","https://openalex.org/W2067344704","https://openalex.org/W2069281048","https://openalex.org/W2074398939","https://openalex.org/W2076710572","https://openalex.org/W2077694535","https://openalex.org/W2078090267","https://openalex.org/W2080615503","https://openalex.org/W2082810430","https://openalex.org/W2084403547","https://openalex.org/W2085756960","https://openalex.org/W2085790749","https://openalex.org/W2087596838","https://openalex.org/W2087802924","https://openalex.org/W2092597147","https://openalex.org/W2092759716","https://openalex.org/W2094795801","https://openalex.org/W2095076911","https://openalex.org/W2101941602","https://openalex.org/W2102277728","https://openalex.org/W2105006060","https://openalex.org/W2106562564","https://openalex.org/W2108102848","https://openalex.org/W2112187315","https://openalex.org/W2115342107","https://openalex.org/W2123388642","https://openalex.org/W2128493116","https://openalex.org/W2130983257","https://openalex.org/W2131097645","https://openalex.org/W2133566253","https://openalex.org/W2133991699","https://openalex.org/W2138774303","https://openalex.org/W2142337964","https://openalex.org/W2148723336","https://openalex.org/W2149285542","https://openalex.org/W2149801992","https://openalex.org/W2151274733","https://openalex.org/W2154741571","https://openalex.org/W2155017982","https://openalex.org/W2155027540","https://openalex.org/W2155708268","https://openalex.org/W2155748850","https://openalex.org/W2156311828","https://openalex.org/W2160648230","https://openalex.org/W2163891447","https://openalex.org/W2163921563","https://openalex.org/W2171755502","https://openalex.org/W2208871739","https://openalex.org/W2334831028","https://openalex.org/W2403963627","https://openalex.org/W2418534521","https://openalex.org/W2422786079","https://openalex.org/W2515574711","https://openalex.org/W2523145772","https://openalex.org/W2559251699","https://openalex.org/W2592966007","https://openalex.org/W2770011498","https://openalex.org/W2773426794","https://openalex.org/W2786092929","https://openalex.org/W2787023214","https://openalex.org/W2800010716","https://openalex.org/W2889727704","https://openalex.org/W2891545684","https://openalex.org/W2892361744","https://openalex.org/W2902940391","https://openalex.org/W2904247712","https://openalex.org/W2913763077","https://openalex.org/W2950629960","https://openalex.org/W2951012447","https://openalex.org/W2953085595","https://openalex.org/W2954897203","https://openalex.org/W2963938814","https://openalex.org/W2964805681","https://openalex.org/W2967811232","https://openalex.org/W2969224557","https://openalex.org/W2970563111","https://openalex.org/W2989859371","https://openalex.org/W2989903733","https://openalex.org/W3082226651","https://openalex.org/W3084521610","https://openalex.org/W3095797616","https://openalex.org/W3110072655","https://openalex.org/W3136074695","https://openalex.org/W3150248096","https://openalex.org/W3169084409","https://openalex.org/W3194293674","https://openalex.org/W3200432307","https://openalex.org/W3204715437","https://openalex.org/W3208340239","https://openalex.org/W3208533785","https://openalex.org/W4200630185","https://openalex.org/W4205465857","https://openalex.org/W4220892275","https://openalex.org/W4226027898","https://openalex.org/W4234689925","https://openalex.org/W4236905934","https://openalex.org/W4280546860","https://openalex.org/W4280613601","https://openalex.org/W4302023449","https://openalex.org/W4303958719","https://openalex.org/W4308522341","https://openalex.org/W4318984781"],"related_works":["https://openalex.org/W3027660895","https://openalex.org/W2088030020","https://openalex.org/W4406844110","https://openalex.org/W2791805485","https://openalex.org/W3095797616","https://openalex.org/W1999473498","https://openalex.org/W4302023449","https://openalex.org/W1987374645","https://openalex.org/W2402328199","https://openalex.org/W1639220408"],"abstract_inverted_index":{"The":[0,108],"advent":[1],"of":[2,15,23,51,87,124,140,159,181,191],"atomic":[3],"force":[4,18,49,69,117],"microscopy,":[5],"along":[6],"with":[7,112],"optical":[8],"tweezers,":[9],"ushered":[10],"in":[11,101,135],"a":[12,24,28,52,57,67,88,102,141,147,174,182],"new":[13,175],"field":[14],"single":[16,25,53,89],"molecule":[17],"spectroscopy,":[19],"wherein":[20],"the":[21,47,61,72,82,97,115,119,125,157],"response":[22,86,123],"protein":[26,54,63,184,192],"or":[27],"macromolecule":[29],"to":[30,41,45,66,70,80,104,114,128,155],"external":[31],"mechanical":[32],"perturbations":[33],"is":[34,64],"measured.":[35],"Controlled":[36],"forces":[37,94],"ranging":[38],"from":[39],"pN":[40],"nN":[42],"are":[43],"applied":[44,116],"measure":[46,71,81,156],"unfolding":[48,73],"distribution":[50],"domain.":[55],"In":[56,149],"clamp":[58],"type":[59],"experiment,":[60],"folded":[62,142,183],"subjected":[65],"constant":[68],"time":[74],"distribution.":[75],"Simultaneously,":[76],"there":[77],"were":[78],"efforts":[79,154],"elastic":[83,120],"and":[84,95,121,185,194],"viscous":[85,122],"domain":[90],"by":[91],"applying":[92],"sinusoidal":[93],"measuring":[96],"resulting":[98],"deformations":[99],"produced":[100],"bid":[103],"quantify":[105],"its":[106,186],"viscoelasticity.":[107],"deformation's":[109],"phase":[110],"lag":[111],"respect":[113],"provides":[118],"protein,":[126],"akin":[127],"oscillatory":[129],"rheology.":[130],"Despite":[131],"numerous":[132],"technical":[133],"advances":[134],"AFM,":[136,163],"an":[137],"artefact-free":[138],"measurement":[139],"protein's":[143],"viscoelasticity":[144,158,180],"largely":[145],"remains":[146],"challenge.":[148],"this":[150],"perspective,":[151],"we":[152,172],"review":[153],"proteins":[160],"using":[161],"dynamic":[162],"identifying":[164],"pitfalls":[165],"that":[166],"make":[167],"these":[168],"measurements":[169],"elusive.":[170],"Finally,":[171],"discuss":[173],"promising":[176],"method,":[177],"which":[178],"reported":[179],"implications":[187],"for":[188],"our":[189],"understanding":[190],"dynamics":[193],"structural":[195],"flexibility.":[196]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2024,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2023-05-17T00:00:00"}