{"id":"https://openalex.org/W4366351201","doi":"https://doi.org/10.1002/bab.2468","title":"Modulation of the catalytic performance of OYE3 by engineering key residues at the entrance of the catalytic pocket","display_name":"Modulation of the catalytic performance of OYE3 by engineering key residues at the entrance of the catalytic pocket","publication_year":2023,"publication_date":"2023-04-19","ids":{"openalex":"https://openalex.org/W4366351201","doi":"https://doi.org/10.1002/bab.2468","pmid":"https://pubmed.ncbi.nlm.nih.gov/37073879"},"language":"en","primary_location":{"id":"doi:10.1002/bab.2468","doi":"https://doi.org/10.1002/bab.2468","is_oa":false,"landing_page_url":"https://doi.org/10.1002/bab.2468","pdf_url":null,"source":{"id":"https://openalex.org/S34872747","display_name":"Biotechnology and Applied Biochemistry","issn_l":"0885-4513","issn":["0885-4513","1470-8744"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Applied Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5045811758","display_name":"Shijin Wu","orcid":"https://orcid.org/0000-0002-0478-2973","observed_orcids":["https://orcid.org/0000-0002-0478-2973"]},"institutions":[{"id":"https://openalex.org/I55712492","display_name":"Zhejiang University of Technology","ror":"https://ror.org/02djqfd08","country_code":"CN","type":"education","lineage":["https://openalex.org/I55712492"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Shijin Wu","raw_affiliation_strings":["College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, China","institution_ids":["https://openalex.org/I55712492"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101979077","display_name":"Qiang Wang","orcid":"https://orcid.org/0000-0003-4392-0889","observed_orcids":["https://orcid.org/0000-0003-4392-0889","https://orcid.org/0000-0002-2986-967X","https://orcid.org/0009-0006-0335-8683"]},"institutions":[{"id":"https://openalex.org/I55712492","display_name":"Zhejiang University of Technology","ror":"https://ror.org/02djqfd08","country_code":"CN","type":"education","lineage":["https://openalex.org/I55712492"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Qiang Wang","raw_affiliation_strings":["College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, China","institution_ids":["https://openalex.org/I55712492"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100549428","display_name":"Xiaojing Ma","orcid":"https://orcid.org/0009-0001-8303-6660","observed_orcids":["https://orcid.org/0009-0001-8303-6660"]},"institutions":[{"id":"https://openalex.org/I55712492","display_name":"Zhejiang University of Technology","ror":"https://ror.org/02djqfd08","country_code":"CN","type":"education","lineage":["https://openalex.org/I55712492"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xiaojing Ma","raw_affiliation_strings":["College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, China","institution_ids":["https://openalex.org/I55712492"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5058476810","display_name":"Lequan Qiu","orcid":"https://orcid.org/0000-0003-4488-7814","observed_orcids":["https://orcid.org/0000-0003-4488-7814"]},"institutions":[{"id":"https://openalex.org/I55712492","display_name":"Zhejiang University of Technology","ror":"https://ror.org/02djqfd08","country_code":"CN","type":"education","lineage":["https://openalex.org/I55712492"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Lequan Qiu","raw_affiliation_strings":["College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, China","institution_ids":["https://openalex.org/I55712492"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5043496775","display_name":"Hongde Yan","orcid":"https://orcid.org/0000-0002-8099-5225","observed_orcids":["https://orcid.org/0000-0002-8099-5225"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Hongde Yan","raw_affiliation_strings":["College of Pharmaceutical Engineering and Biotechnology, Zhejiang Pharmaceutical University, Ningbo, China"],"raw_orcid":"https://orcid.org/0000-0002-8099-5225","affiliations":[{"raw_affiliation_string":"College of Pharmaceutical Engineering and Biotechnology, Zhejiang Pharmaceutical University, Ningbo, China","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5043496775"],"corresponding_institution_ids":[],"apc_list":{"value":3140,"currency":"USD","value_usd":3140},"apc_paid":null,"fwci":0.4449,"has_fulltext":false,"cited_by_count":6,"citation_normalized_percentile":{"value":0.48929901,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":97},"biblio":{"volume":"70","issue":"5","first_page":"1720","last_page":"1730"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11281","display_name":"Asymmetric Hydrogenation and Catalysis","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1604","display_name":"Inorganic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/site-directed-mutagenesis","display_name":"site-directed mutagenesis","score":0.7689999938011169},{"id":"https://openalex.org/keywords/catalytic-pocket","display_name":"catalytic pocket","score":0.746999979019165},{"id":"https://openalex.org/keywords/steric-hindrance","display_name":"steric hindrance","score":0.9020000100135803},{"id":"https://openalex.org/keywords/stereoselective-reduction","display_name":"stereoselective reduction","score":0.7260000109672546},{"id":"https://openalex.org/keywords/diastereoselectivity","display_name":"diastereoselectivity","score":0.9079999923706055},{"id":"https://openalex.org/keywords/rational-enzyme-design","display_name":"rational enzyme design","score":0.7319999933242798},{"id":"https://openalex.org/keywords/saccharomyces-cerevisiae","display_name":"Saccharomyces cerevisiae","score":0.8930000066757202}],"concepts":[{"id":"https://openalex.org/C181647583","wikidata":"https://www.wikidata.org/wiki/Q903429","display_name":"Stereoselectivity","level":3,"score":0.7811087369918823},{"id":"https://openalex.org/C2781338088","wikidata":"https://www.wikidata.org/wiki/Q903495","display_name":"Residue (chemistry)","level":2,"score":0.7442105412483215},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7393848896026611},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.7095514535903931},{"id":"https://openalex.org/C201194858","wikidata":"https://www.wikidata.org/wiki/Q898238","display_name":"Steric effects","level":2,"score":0.640849232673645},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.598387598991394},{"id":"https://openalex.org/C73969872","wikidata":"https://www.wikidata.org/wiki/Q899698","display_name":"Turnover number","level":3,"score":0.5074037909507751},{"id":"https://openalex.org/C2781370763","wikidata":"https://www.wikidata.org/wiki/Q60176530","display_name":"Citral","level":3,"score":0.4947957396507263},{"id":"https://openalex.org/C161790260","wikidata":"https://www.wikidata.org/wiki/Q82264","display_name":"Catalysis","level":2,"score":0.45607689023017883},{"id":"https://openalex.org/C41183919","wikidata":"https://www.wikidata.org/wiki/Q423026","display_name":"Active site","level":3,"score":0.4513704478740692},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.28194206953048706},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.05448290705680847},{"id":"https://openalex.org/C2777278459","wikidata":"https://www.wikidata.org/wiki/Q170885","display_name":"Essential oil","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000081005","descriptor_name":"Cyclohexane Monoterpenes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000596","descriptor_name":"Amino Acids","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D002384","descriptor_name":"Catalysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009252","descriptor_name":"NADPH Dehydrogenase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D009252","descriptor_name":"NADPH Dehydrogenase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D009252","descriptor_name":"NADPH Dehydrogenase","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D012441","descriptor_name":"Saccharomyces cerevisiae","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1002/bab.2468","doi":"https://doi.org/10.1002/bab.2468","is_oa":false,"landing_page_url":"https://doi.org/10.1002/bab.2468","pdf_url":null,"source":{"id":"https://openalex.org/S34872747","display_name":"Biotechnology and Applied Biochemistry","issn_l":"0885-4513","issn":["0885-4513","1470-8744"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Applied Biochemistry","raw_type":"journal-article"},{"id":"pmid:37073879","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/37073879","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and applied biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G4586135925","display_name":null,"funder_award_id":"LGF22E060004","funder_id":"https://openalex.org/F3057939197","funder_display_name":"Comanche Nation"}],"funders":[{"id":"https://openalex.org/F3057939197","display_name":"Comanche Nation","ror":"https://ror.org/039rx3k87"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":30,"referenced_works":["https://openalex.org/W1996685220","https://openalex.org/W2046228636","https://openalex.org/W2056471647","https://openalex.org/W2112499938","https://openalex.org/W2126470506","https://openalex.org/W2128956131","https://openalex.org/W2136403432","https://openalex.org/W2144742694","https://openalex.org/W2159733930","https://openalex.org/W2160226556","https://openalex.org/W2228371896","https://openalex.org/W2318797004","https://openalex.org/W2326027917","https://openalex.org/W2586925363","https://openalex.org/W2610433166","https://openalex.org/W2767423571","https://openalex.org/W2781622269","https://openalex.org/W2792554123","https://openalex.org/W2804040157","https://openalex.org/W2889519153","https://openalex.org/W2900824499","https://openalex.org/W2922309965","https://openalex.org/W2938150044","https://openalex.org/W2942491534","https://openalex.org/W3081031998","https://openalex.org/W3188327903","https://openalex.org/W3195369473","https://openalex.org/W3204310478","https://openalex.org/W4210856111","https://openalex.org/W4281726681"],"related_works":["https://openalex.org/W2922309965","https://openalex.org/W2326027917","https://openalex.org/W2938150044","https://openalex.org/W2057550086","https://openalex.org/W3195369473","https://openalex.org/W2033240308","https://openalex.org/W2318797004","https://openalex.org/W2901819957","https://openalex.org/W2160226556","https://openalex.org/W3081031998"],"abstract_inverted_index":{"The":[0,54,91],"amino":[1,52],"acid":[2],"residues":[3,45],"at":[4],"the":[5,8,16,20,24,28,31,35,60,67,76,84,87,94,103,118,134,147,158,170,178,181],"entrance":[6],"of":[7,23,30,34,59,78,89,93,120,136,146,160,169,180,192],"catalytic":[9,68],"pocket":[10],"may":[11],"impose":[12],"steric":[13],"hindrance":[14],"on":[15,27,66,102,177],"substrate":[17],"to":[18,50],"enter":[19],"active":[21],"center":[22],"enzyme.":[25,182],"Based":[26],"analysis":[29],"three-dimensional":[32],"structure":[33],"Saccharomyces":[36],"cerevisiae":[37],"old":[38],"yellow":[39],"enzyme":[40,190],"3":[41],"(OYE3),":[42],"four":[43,71],"bulky":[44],"were":[46],"chosen":[47],"and":[48,80,105,110,115,128,131,154],"mutated":[49],"small":[51],"acids.":[53],"results":[55],"showed":[56,112],"that":[57],"mutation":[58,92],"W116":[61],"residue":[62,96,172],"had":[63,97,173],"interesting":[64],"impacts":[65],"performance.":[69],"All":[70],"variants":[72],"became":[73],"inactive":[74],"for":[75,86,117,133,157,188],"reduction":[77,88,119,135,159],"(R)-carvone":[79,121,161],"(S)-carvone,":[81],"but":[82],"inverted":[83],"stereoselectivity":[85],"(E/Z)-citral.":[90],"F250":[95],"a":[98,174],"more":[99],"positive":[100],"effect":[101],"activity":[104,116,132,155,179],"stereoselectivity.":[106],"Two":[107],"variants,":[108],"F250A":[109],"F250S,":[111],"excellent":[113,152],"diastereoselectivity":[114,130,153],"(de":[122,138,162],">":[123,126,139,142,163,166],"99%,":[124,164],"c":[125,141,165],"99%)":[127],"increased":[129],"(S)-carvone":[137],"96%,":[140],"80%).":[143],"One":[144],"variant":[145],"P295":[148],"residue,":[149],"P295G,":[150],"displayed":[151],"only":[156],"99%).":[167],"Mutation":[168],"Y375":[171],"negative":[175],"impact":[176],"These":[183],"findings":[184],"provide":[185],"some":[186],"solutions":[187],"rational":[189],"engineering":[191],"OYE3.":[193]},"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":2}],"updated_date":"2026-11-12T12:28:57.059296","created_date":"2023-04-21T00:00:00"}