{"id":"https://openalex.org/W4366285672","doi":"https://doi.org/10.18502/jri.v24i2.12492","title":"Comparison of In-House Microfluidic Device and Centrifuge-Based Method Efficacy in Sperm Preparation for Assisted Reproductive Technology","display_name":"Comparison of In-House Microfluidic Device and Centrifuge-Based Method Efficacy in Sperm Preparation for Assisted Reproductive Technology","publication_year":2023,"publication_date":"2023-04-18","ids":{"openalex":"https://openalex.org/W4366285672","doi":"https://doi.org/10.18502/jri.v24i2.12492","pmid":"https://pubmed.ncbi.nlm.nih.gov/37547571","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/10402458"},"language":"en","primary_location":{"id":"doi:10.18502/jri.v24i2.12492","doi":"https://doi.org/10.18502/jri.v24i2.12492","is_oa":false,"landing_page_url":"https://doi.org/10.18502/jri.v24i2.12492","pdf_url":null,"source":{"id":"https://openalex.org/S2764457158","display_name":"Journal of Reproduction & Infertility","issn_l":"2228-5482","issn":["2228-5482","2251-676X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","norway-1","poland-40","russia-white-list-1"],"host_organization":"https://openalex.org/P4310319396","host_organization_name":"Knowledge E","host_organization_lineage":["https://openalex.org/P4310319396"],"host_organization_lineage_names":["Knowledge E"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Reproduction & Infertility","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC10402458/pdf/JRI-24-85.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5033605963","display_name":"Tonghathai Phairatana","orcid":"https://orcid.org/0000-0002-1372-6471","observed_orcids":["https://orcid.org/0000-0002-1372-6471"]},"institutions":[{"id":"https://openalex.org/I131868736","display_name":"Prince of Songkla University","ror":"https://ror.org/0575ycz84","country_code":"TH","type":"education","lineage":["https://openalex.org/I131868736"]}],"countries":["TH"],"is_corresponding":false,"raw_author_name":"Tonghathai Phairatana","raw_affiliation_strings":["Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand","institution_ids":["https://openalex.org/I131868736"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5060787866","display_name":"Thanaporn Prateepchaikul","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I131868736","display_name":"Prince of Songkla University","ror":"https://ror.org/0575ycz84","country_code":"TH","type":"education","lineage":["https://openalex.org/I131868736"]}],"countries":["TH"],"is_corresponding":false,"raw_author_name":"Thanaporn Prateepchaikul","raw_affiliation_strings":["Department of Obstetrics and Gynecology, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Obstetrics and Gynecology, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand","institution_ids":["https://openalex.org/I131868736"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5034818062","display_name":"Raphatphorn Navakanitworakul","orcid":"https://orcid.org/0000-0001-6408-0079","observed_orcids":["https://orcid.org/0000-0001-6408-0079"]},"institutions":[{"id":"https://openalex.org/I131868736","display_name":"Prince of Songkla University","ror":"https://ror.org/0575ycz84","country_code":"TH","type":"education","lineage":["https://openalex.org/I131868736"]}],"countries":["TH"],"is_corresponding":false,"raw_author_name":"Raphatphorn Navakanittworakul","raw_affiliation_strings":["Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand","institution_ids":["https://openalex.org/I131868736"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5086337263","display_name":"Chainarong Choksuchat","orcid":"https://orcid.org/0000-0003-4392-4070","observed_orcids":["https://orcid.org/0000-0003-4392-4070"]},"institutions":[{"id":"https://openalex.org/I131868736","display_name":"Prince of Songkla University","ror":"https://ror.org/0575ycz84","country_code":"TH","type":"education","lineage":["https://openalex.org/I131868736"]}],"countries":["TH"],"is_corresponding":true,"raw_author_name":"Chainarong Choksuchat","raw_affiliation_strings":["Department of Obstetrics and Gynecology, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Obstetrics and Gynecology, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand","institution_ids":["https://openalex.org/I131868736"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5086337263"],"corresponding_institution_ids":["https://openalex.org/I131868736"],"apc_list":null,"apc_paid":null,"fwci":1.1996,"has_fulltext":true,"cited_by_count":5,"citation_normalized_percentile":{"value":0.76884621,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":98},"biblio":{"volume":"24","issue":"2","first_page":"85","last_page":"93"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10116","display_name":"Sperm and Testicular Function","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10116","display_name":"Sperm and Testicular Function","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11156","display_name":"Reproductive Health and Technologies","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T13242","display_name":"Male Reproductive Health Studies","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/3600","display_name":"General Health Professions"},"field":{"id":"https://openalex.org/fields/36","display_name":"Health Professions"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/sperm-preparation","display_name":"sperm preparation","score":0.7889999747276306},{"id":"https://openalex.org/keywords/microfluidic-sperm-selection","display_name":"microfluidic sperm selection","score":0.8690000176429749},{"id":"https://openalex.org/keywords/density-gradient-centrifugation","display_name":"density gradient centrifugation","score":0.9710000157356262},{"id":"https://openalex.org/keywords/sperm-dna-fragmentation","display_name":"sperm DNA fragmentation","score":0.9539999961853027},{"id":"https://openalex.org/keywords/assisted-reproductive-technology","display_name":"assisted reproduction","score":0.8500000238418579},{"id":"https://openalex.org/keywords/sperm-motility","display_name":"sperm motility","score":0.8080000281333923},{"id":"https://openalex.org/keywords/sperm-morphology","display_name":"sperm morphology","score":0.9399999976158142},{"id":"https://openalex.org/keywords/tunel-assay","display_name":"TUNEL","score":0.8669999837875366}],"concepts":[{"id":"https://openalex.org/C43759708","wikidata":"https://www.wikidata.org/wiki/Q3749256","display_name":"DNA fragmentation","level":4,"score":0.7956571578979492},{"id":"https://openalex.org/C2781087480","wikidata":"https://www.wikidata.org/wiki/Q17145","display_name":"Sperm","level":2,"score":0.7763644456863403},{"id":"https://openalex.org/C2778093475","wikidata":"https://www.wikidata.org/wiki/Q9715","display_name":"Semen","level":2,"score":0.7648162841796875},{"id":"https://openalex.org/C16685009","wikidata":"https://www.wikidata.org/wiki/Q335057","display_name":"Andrology","level":1,"score":0.6553400754928589},{"id":"https://openalex.org/C2777632594","wikidata":"https://www.wikidata.org/wiki/Q27116486","display_name":"Sperm motility","level":3,"score":0.6269305944442749},{"id":"https://openalex.org/C7623868","wikidata":"https://www.wikidata.org/wiki/Q1967135","display_name":"Centrifugation","level":2,"score":0.5344246029853821},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5095891952514648},{"id":"https://openalex.org/C58207958","wikidata":"https://www.wikidata.org/wiki/Q33596","display_name":"Motility","level":2,"score":0.47386813163757324},{"id":"https://openalex.org/C8673954","wikidata":"https://www.wikidata.org/wiki/Q138845","display_name":"Microfluidics","level":2,"score":0.45771709084510803},{"id":"https://openalex.org/C2777589648","wikidata":"https://www.wikidata.org/wiki/Q1501844","display_name":"Semen analysis","level":4,"score":0.4325277507305145},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.33336883783340454},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.31896060705184937},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.2502959668636322},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.15101373195648193},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.12241721153259277},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.12020361423492432},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.09633412957191467},{"id":"https://openalex.org/C190283241","wikidata":"https://www.wikidata.org/wiki/Q14599311","display_name":"Apoptosis","level":2,"score":0.09358838200569153},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.08618524670600891},{"id":"https://openalex.org/C2777688143","wikidata":"https://www.wikidata.org/wiki/Q179399","display_name":"Infertility","level":3,"score":0.07678765058517456},{"id":"https://openalex.org/C2779234561","wikidata":"https://www.wikidata.org/wiki/Q11995","display_name":"Pregnancy","level":2,"score":0.0},{"id":"https://openalex.org/C31573885","wikidata":"https://www.wikidata.org/wiki/Q304484","display_name":"Programmed cell death","level":3,"score":0.0}],"mesh":[],"locations_count":3,"locations":[{"id":"doi:10.18502/jri.v24i2.12492","doi":"https://doi.org/10.18502/jri.v24i2.12492","is_oa":false,"landing_page_url":"https://doi.org/10.18502/jri.v24i2.12492","pdf_url":null,"source":{"id":"https://openalex.org/S2764457158","display_name":"Journal of Reproduction & Infertility","issn_l":"2228-5482","issn":["2228-5482","2251-676X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","norway-1","poland-40","russia-white-list-1"],"host_organization":"https://openalex.org/P4310319396","host_organization_name":"Knowledge E","host_organization_lineage":["https://openalex.org/P4310319396"],"host_organization_lineage_names":["Knowledge E"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Reproduction & Infertility","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:10402458","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/10402458","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC10402458/pdf/JRI-24-85.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Reprod Infertil","raw_type":"Text"},{"id":"pmid:37547571","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/37547571","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of reproduction & infertility","raw_type":"Journal Article"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:10402458","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/10402458","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC10402458/pdf/JRI-24-85.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Reprod Infertil","raw_type":"Text"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.6240000128746033}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320322692","display_name":"Prince of Songkla University","ror":"https://ror.org/0575ycz84"},{"id":"https://openalex.org/F4320326657","display_name":"Faculty of Medicine, Prince of Songkla University","ror":null}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4366285672.pdf","grobid_xml":"https://content.openalex.org/works/W4366285672.grobid-xml"},"referenced_works_count":28,"referenced_works":["https://openalex.org/W1976266332","https://openalex.org/W1977726227","https://openalex.org/W1987119501","https://openalex.org/W2011194136","https://openalex.org/W2041661126","https://openalex.org/W2047270772","https://openalex.org/W2067126948","https://openalex.org/W2096036771","https://openalex.org/W2107825545","https://openalex.org/W2113164000","https://openalex.org/W2141565053","https://openalex.org/W2154379294","https://openalex.org/W2156101504","https://openalex.org/W2194476799","https://openalex.org/W2413172437","https://openalex.org/W2553053290","https://openalex.org/W2739504526","https://openalex.org/W2767411585","https://openalex.org/W2778491596","https://openalex.org/W2847112742","https://openalex.org/W2942070576","https://openalex.org/W2946436563","https://openalex.org/W2978813803","https://openalex.org/W2984566128","https://openalex.org/W2991105897","https://openalex.org/W3029212823","https://openalex.org/W4205767333","https://openalex.org/W4211005481"],"related_works":["https://openalex.org/W4391358367","https://openalex.org/W4411750639","https://openalex.org/W7131869790","https://openalex.org/W3037890008","https://openalex.org/W7024122409","https://openalex.org/W7140206915","https://openalex.org/W7008734457","https://openalex.org/W7167676120","https://openalex.org/W4283735945","https://openalex.org/W3193750786"],"abstract_inverted_index":{"Background:":[0],"Sperm":[1,94,116],"DNA":[2,36,103],"fragmentation":[3,37,104],"can":[4],"affect":[5],"reproductive":[6,10],"outcomes":[7],"in":[8,18,56,70,133,160],"assisted":[9],"techniques":[11],"(ART),":[12],"and":[13,84,132,141,191],"it":[14],"is":[15],"a":[16,51,123],"concern":[17],"density":[19],"gradient":[20],"centrifugation":[21],"(DGC).":[22],"By":[23],"contrast,":[24],"microfluidic":[25,52,92,120,146,176],"approaches":[26],"allow":[27],"the":[28,48,71,91,97,106,119,145,174,198],"selection":[29],"of":[30,42,50,96,138],"highly":[31],"motile":[32],"sperm":[33,80,151,178],"with":[34,58,64,86,102,136,179,187],"low":[35],"index":[38],"(DFI).":[39],"The":[40,189],"purpose":[41],"current":[43],"study,":[44],"was":[45,182],"to":[46,87],"compare":[47],"efficacy":[49],"device":[53],"designed":[54],"in-house":[55,175],"comparison":[57],"DGC.":[59,188],"Methods:":[60],"Nineteen":[61],"healthy":[62],"men":[63],"normal":[65,170,192],"semen":[66],"profiles":[67],"were":[68,75,99,158,195],"included":[69],"study.":[72],"Semen":[73],"samples":[74,98],"individually":[76],"aliquoted":[77],"for":[78],"three":[79],"preparation":[81],"analyses":[82],"(crude":[83],"processed":[85,117],"either":[88],"DGC":[89,131],"or":[90,169],"method).":[93],"parameters":[95],"evaluated":[100],"along":[101],"using":[105,118,130],"terminal":[107],"deoxynucleotidyl":[108],"transferase":[109],"dUTP":[110],"nick-end":[111],"labeling":[112],"(TUNEL)":[113],"method.":[114],"Results:":[115],"method":[121,147],"showed":[122],"significantly":[124,149],"lower":[125,150,180],"DFI":[126,137,181],"than":[127,153],"those":[128],"obtained":[129],"crude":[134],"semen,":[135],"1.1%,":[139],"3.5%,":[140],"4.9%,":[142],"respectively.":[143],"Although":[144],"yielded":[148],"concentrations":[152],"DGC,":[154],"no":[155],"significant":[156],"differences":[157],"observed":[159],"total":[161],"motility,":[162,164],"progressive":[163],"curvilinear":[165],"velocity,":[166,168],"straight-line":[167],"morphology.":[171],"Conclusion:":[172],"Using":[173],"device,":[177],"effectively":[183],"isolated":[184],"when":[185],"compared":[186],"motility":[190],"morphology":[193],"rates":[194],"comparable":[196],"among":[197],"samples.":[199]},"counts_by_year":[{"year":2025,"cited_by_count":4},{"year":2024,"cited_by_count":1}],"updated_date":"2026-10-29T09:56:32.521200","created_date":"2023-04-20T00:00:00"}