{"id":"https://openalex.org/W4366221053","doi":"https://doi.org/10.3390/molecules28083494","title":"A Novel Peptide Reagent for Investigating Disulfide-Coupled Folding Intermediates of Mid-Size Proteins","display_name":"A Novel Peptide Reagent for Investigating Disulfide-Coupled Folding Intermediates of Mid-Size Proteins","publication_year":2023,"publication_date":"2023-04-15","ids":{"openalex":"https://openalex.org/W4366221053","doi":"https://doi.org/10.3390/molecules28083494","pmid":"https://pubmed.ncbi.nlm.nih.gov/37110728","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/10142513"},"language":"en","primary_location":{"id":"doi:10.3390/molecules28083494","doi":"https://doi.org/10.3390/molecules28083494","is_oa":true,"landing_page_url":"https://doi.org/10.3390/molecules28083494","pdf_url":"https://www.mdpi.com/1420-3049/28/8/3494/pdf?version=1681547806","source":{"id":"https://openalex.org/S108911230","display_name":"Molecules","issn_l":"1420-3049","issn":["1420-3049","1433-1373"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","medline","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecules","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.mdpi.com/1420-3049/28/8/3494/pdf?version=1681547806","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5009679671","display_name":"Nana Sakata","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I916559398","display_name":"Kindai University","ror":"https://ror.org/05kt9ap64","country_code":"JP","type":"education","lineage":["https://openalex.org/I916559398"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Nana Sakata","raw_affiliation_strings":["Faculty of Science and Engineering, Kindai University, 3-4-1 Kowakae, Higashi-Osaka 577-8502, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Faculty of Science and Engineering, Kindai University, 3-4-1 Kowakae, Higashi-Osaka 577-8502, Japan","institution_ids":["https://openalex.org/I916559398"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102974159","display_name":"Yuri Murakami","orcid":"https://orcid.org/0000-0002-8754-5913","observed_orcids":["https://orcid.org/0000-0002-8754-5913"]},"institutions":[{"id":"https://openalex.org/I916559398","display_name":"Kindai University","ror":"https://ror.org/05kt9ap64","country_code":"JP","type":"education","lineage":["https://openalex.org/I916559398"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Yuri Murakami","raw_affiliation_strings":["Faculty of Science and Engineering, Kindai University, 3-4-1 Kowakae, Higashi-Osaka 577-8502, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Faculty of Science and Engineering, Kindai University, 3-4-1 Kowakae, Higashi-Osaka 577-8502, Japan","institution_ids":["https://openalex.org/I916559398"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110966535","display_name":"Mitsuhiro Miyazawa","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1323638106","display_name":"National Agriculture and Food Research Organization","ror":"https://ror.org/023v4bd62","country_code":"JP","type":"government","lineage":["https://openalex.org/I1323638106"]},{"id":"https://openalex.org/I3018761593","display_name":"Institute of Agrobiological Sciences","ror":"https://ror.org/01786mp71","country_code":"JP","type":"facility","lineage":["https://openalex.org/I1323638106","https://openalex.org/I3018761593"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Mitsuhiro Miyazawa","raw_affiliation_strings":["Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, Tsukuba 305-8634, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, Tsukuba 305-8634, Japan","institution_ids":["https://openalex.org/I1323638106","https://openalex.org/I3018761593"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5000273424","display_name":"Shigeru Shimamoto","orcid":"https://orcid.org/0000-0002-9470-0352","observed_orcids":["https://orcid.org/0000-0002-9470-0352"]},"institutions":[{"id":"https://openalex.org/I916559398","display_name":"Kindai University","ror":"https://ror.org/05kt9ap64","country_code":"JP","type":"education","lineage":["https://openalex.org/I916559398"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Shigeru Shimamoto","raw_affiliation_strings":["Faculty of Science and Engineering, Kindai University, 3-4-1 Kowakae, Higashi-Osaka 577-8502, Japan"],"raw_orcid":"https://orcid.org/0000-0002-9470-0352","affiliations":[{"raw_affiliation_string":"Faculty of Science and Engineering, Kindai University, 3-4-1 Kowakae, Higashi-Osaka 577-8502, Japan","institution_ids":["https://openalex.org/I916559398"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5005411565","display_name":"Yuji Hidaka","orcid":"https://orcid.org/0000-0002-3131-2619","observed_orcids":["https://orcid.org/0000-0002-3131-2619"]},"institutions":[{"id":"https://openalex.org/I916559398","display_name":"Kindai University","ror":"https://ror.org/05kt9ap64","country_code":"JP","type":"education","lineage":["https://openalex.org/I916559398"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Yuji Hidaka","raw_affiliation_strings":["Faculty of Science and Engineering, Kindai University, 3-4-1 Kowakae, Higashi-Osaka 577-8502, Japan"],"raw_orcid":"https://orcid.org/0000-0002-3131-2619","affiliations":[{"raw_affiliation_string":"Faculty of Science and Engineering, Kindai University, 3-4-1 Kowakae, Higashi-Osaka 577-8502, Japan","institution_ids":["https://openalex.org/I916559398"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5000273424","https://openalex.org/A5005411565"],"corresponding_institution_ids":["https://openalex.org/I916559398"],"apc_list":{"value":2300,"currency":"CHF","value_usd":2558},"apc_paid":{"value":2300,"currency":"CHF","value_usd":2558},"fwci":0.2966,"has_fulltext":true,"cited_by_count":4,"citation_normalized_percentile":{"value":0.40481264,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":95},"biblio":{"volume":"28","issue":"8","first_page":"3494","last_page":"3494"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.9121000170707703,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.9121000170707703,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.038100000470876694,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":0.010200000368058681,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/folding-intermediates","display_name":"folding intermediates","score":0.9440000057220459},{"id":"https://openalex.org/keywords/aprotinin","display_name":"aprotinin","score":0.656000018119812},{"id":"https://openalex.org/keywords/propeptide","display_name":"propeptide","score":0.6669999957084656},{"id":"https://openalex.org/keywords/disulfide-exchange","display_name":"disulfide exchange","score":0.609000027179718},{"id":"https://openalex.org/keywords/folding-mechanism","display_name":"folding mechanism","score":0.35899999737739563}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.8078075051307678},{"id":"https://openalex.org/C40875361","wikidata":"https://www.wikidata.org/wiki/Q2356542","display_name":"Reagent","level":2,"score":0.7531863451004028},{"id":"https://openalex.org/C2776545253","wikidata":"https://www.wikidata.org/wiki/Q5464292","display_name":"Folding (DSP implementation)","level":2,"score":0.6926663517951965},{"id":"https://openalex.org/C204328495","wikidata":"https://www.wikidata.org/wiki/Q847556","display_name":"Protein folding","level":2,"score":0.5628195405006409},{"id":"https://openalex.org/C2779281246","wikidata":"https://www.wikidata.org/wiki/Q172847","display_name":"Peptide","level":2,"score":0.5413004159927368},{"id":"https://openalex.org/C2779201268","wikidata":"https://www.wikidata.org/wiki/Q186474","display_name":"Cysteine","level":3,"score":0.4533764719963074},{"id":"https://openalex.org/C27256138","wikidata":"https://www.wikidata.org/wiki/Q1230226","display_name":"Disulfide bond","level":2,"score":0.4455816149711609},{"id":"https://openalex.org/C21951064","wikidata":"https://www.wikidata.org/wiki/Q899212","display_name":"Combinatorial chemistry","level":1,"score":0.3728207051753998},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.36921894550323486},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.1278211772441864},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.060701847076416016},{"id":"https://openalex.org/C119599485","wikidata":"https://www.wikidata.org/wiki/Q43035","display_name":"Electrical engineering","level":1,"score":0.0},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D002851","descriptor_name":"Chromatography, High Pressure Liquid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004220","descriptor_name":"Disulfides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010084","descriptor_name":"Oxidation-Reduction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010455","descriptor_name":"Peptides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011498","descriptor_name":"Protein Precursors","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D017510","descriptor_name":"Protein Folding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.3390/molecules28083494","doi":"https://doi.org/10.3390/molecules28083494","is_oa":true,"landing_page_url":"https://doi.org/10.3390/molecules28083494","pdf_url":"https://www.mdpi.com/1420-3049/28/8/3494/pdf?version=1681547806","source":{"id":"https://openalex.org/S108911230","display_name":"Molecules","issn_l":"1420-3049","issn":["1420-3049","1433-1373"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","medline","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecules","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:10142513","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/10142513","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC10142513/pdf/molecules-28-03494.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecules","raw_type":"Text"},{"id":"pmid:37110728","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/37110728","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecules (Basel, Switzerland)","raw_type":"Journal Article"},{"id":"pmh:oai:doaj.org/article:2aa2171738c94356b9fcfe7dcdba4c7d","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/2aa2171738c94356b9fcfe7dcdba4c7d","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Molecules, Vol 28, Iss 8, p 3494 (2023)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.3390/molecules28083494","doi":"https://doi.org/10.3390/molecules28083494","is_oa":true,"landing_page_url":"https://doi.org/10.3390/molecules28083494","pdf_url":"https://www.mdpi.com/1420-3049/28/8/3494/pdf?version=1681547806","source":{"id":"https://openalex.org/S108911230","display_name":"Molecules","issn_l":"1420-3049","issn":["1420-3049","1433-1373"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","medline","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecules","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G141052899","display_name":"substrate-induced product-release mechanism of L-PGDS","funder_award_id":"16K18868","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"},{"id":"https://openalex.org/G8334617811","display_name":"Molecular evolution of precursor protein of peptide hormone and its application for molecular modeling","funder_award_id":"16K01925","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"}],"funders":[{"id":"https://openalex.org/F4320313220","display_name":"Kindai University","ror":"https://ror.org/05kt9ap64"},{"id":"https://openalex.org/F4320318231","display_name":"Kanazawa University","ror":"https://ror.org/02hwp6a56"},{"id":"https://openalex.org/F4320334764","display_name":"Japan Society for the Promotion of Science","ror":"https://ror.org/00hhkn466"},{"id":"https://openalex.org/F4320336670","display_name":"Institute for Protein Research, Osaka University","ror":null}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4366221053.pdf","grobid_xml":"https://content.openalex.org/works/W4366221053.grobid-xml"},"referenced_works_count":30,"referenced_works":["https://openalex.org/W1487070220","https://openalex.org/W1498774601","https://openalex.org/W1516956925","https://openalex.org/W1525157964","https://openalex.org/W1546609096","https://openalex.org/W1585700996","https://openalex.org/W1597549047","https://openalex.org/W1782328228","https://openalex.org/W2004091546","https://openalex.org/W2019139743","https://openalex.org/W2020446519","https://openalex.org/W2041484038","https://openalex.org/W2055155126","https://openalex.org/W2073084525","https://openalex.org/W2074037462","https://openalex.org/W2077664075","https://openalex.org/W2084509221","https://openalex.org/W2101912278","https://openalex.org/W2125641354","https://openalex.org/W2312588983","https://openalex.org/W2320556745","https://openalex.org/W2602603832","https://openalex.org/W2887050230","https://openalex.org/W2994480032","https://openalex.org/W3093638118","https://openalex.org/W3103439224","https://openalex.org/W4286512926","https://openalex.org/W4296698543","https://openalex.org/W4309683841","https://openalex.org/W4317738351"],"related_works":["https://openalex.org/W7130580863","https://openalex.org/W2015561631","https://openalex.org/W2312588983","https://openalex.org/W2293750593","https://openalex.org/W410297436","https://openalex.org/W2044873534","https://openalex.org/W1855896685","https://openalex.org/W2091405943","https://openalex.org/W1487070220","https://openalex.org/W2036087909"],"abstract_inverted_index":{"Investigations":[0],"of":[1,9,16,30,34,41,67,70,85,99,131,139,150,207,228,231],"protein":[2,80,97],"folding":[3,15,19,32,46,68,91,138,148,154,169,230],"have":[4],"largely":[5],"involved":[6],"the":[7,13,31,65,83,86,128,137,147,153,168,173,188,192,205,212,226],"use":[8],"disulfide-containing":[10],"proteins,":[11],"since":[12,152],"disulfide-coupled":[14,229],"proteins":[17,36],"allows":[18],"intermediates":[20,47,69,155,170,193],"to":[21,51,64,81,89,145,166,179,194],"be":[22,158,195],"trapped":[23],"and":[24,62,117,200],"their":[25],"conformations":[26],"determined.":[27],"However,":[28,141],"studies":[29],"mechanisms":[33,227],"mid-size":[35,108,232],"face":[37],"several":[38],"problems,":[39],"one":[40],"which":[42],"is":[43,48,111,134,220],"that":[44,127,187],"detecting":[45],"difficult.":[49],"Therefore,":[50,165],"solve":[52],"this":[53,181],"issue,":[54],"a":[55,77,95,106,114,119,161,221],"novel":[56,87,174],"peptide":[57,189,216],"reagent,":[58],"maleimidohexanoyl-Arg5-Tyr-NH2,":[59],"was":[60,74,103,143,177],"designed":[61],"applied":[63],"detection":[66],"model":[71,78,107],"proteins.":[72,233],"BPTI":[73],"chosen":[75],"as":[76,105,113],"small":[79],"estimate":[82],"ability":[84],"reagent":[88,176,190,217],"detect":[90],"intermediates.":[92],"In":[93],"addition,":[94],"precursor":[96],"(prococoonase)":[98],"Bombyx":[100],"mori":[101],"cocoonase":[102],"used":[104,178],"protein.":[109],"Cocoonase":[110],"classified":[112],"serine":[115],"protease":[116],"has":[118],"high":[120],"homology":[121],"with":[122],"trypsin.":[123],"We":[124],"recently":[125],"found":[126],"propeptide":[129],"sequence":[130],"prococoonase":[132],"(proCCN)":[133],"important":[135],"for":[136,182,224],"cocoonase.":[140],"it":[142],"difficult":[144],"study":[146],"pathway":[149],"proCCN":[151],"could":[156],"not":[157],"separated":[159,197],"on":[160,198],"reversed-phase":[162],"HPLC":[163],"(RP-HPLC).":[164],"separate":[167],"by":[171,202],"RP-HPLC,":[172],"labeling":[175,213],"accomplish":[180],"proCCN.":[183],"The":[184,215],"results":[185],"indicated":[186],"allowed":[191],"captured,":[196],"SDS-PAGE,":[199],"analyzed":[201],"RP-HPLC":[203],"without":[204],"occurrence":[206],"undesirable":[208],"disulfide-exchange":[209],"reactions":[210],"during":[211],"reactions.":[214],"reported":[218],"herein":[219],"practical":[222],"tool":[223],"investigating":[225]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2023-04-19T00:00:00"}