{"id":"https://openalex.org/W4366208117","doi":"https://doi.org/10.3389/fmicb.2023.1161764","title":"Bioinformatic identification of ClpI, a distinct class of Clp unfoldases in Actinomycetota","display_name":"Bioinformatic identification of ClpI, a distinct class of Clp unfoldases in Actinomycetota","publication_year":2023,"publication_date":"2023-04-17","ids":{"openalex":"https://openalex.org/W4366208117","doi":"https://doi.org/10.3389/fmicb.2023.1161764","pmid":"https://pubmed.ncbi.nlm.nih.gov/37138635","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/10149685"},"language":"en","primary_location":{"id":"doi:10.3389/fmicb.2023.1161764","doi":"https://doi.org/10.3389/fmicb.2023.1161764","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fmicb.2023.1161764","pdf_url":null,"source":{"id":"https://openalex.org/S2484704948","display_name":"Frontiers in Microbiology","issn_l":"1664-302X","issn":["1664-302X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Microbiology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.3389/fmicb.2023.1161764","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5078731855","display_name":"Jialiu Jiang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I86501945","display_name":"University of Delaware","ror":"https://ror.org/01sbq1a82","country_code":"US","type":"education","lineage":["https://openalex.org/I86501945"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jialiu Jiang","raw_affiliation_strings":["Department of Chemistry & Biochemistry, University of Delaware, Newark DE, 19716","Department of Chemistry and Biochemistry, University of Delaware, Newark, DE, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry & Biochemistry, University of Delaware, Newark DE, 19716","institution_ids":["https://openalex.org/I86501945"]},{"raw_affiliation_string":"Department of Chemistry and Biochemistry, University of Delaware, Newark, DE, United States","institution_ids":["https://openalex.org/I86501945"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5079741827","display_name":"Karl R. Schmitz","orcid":"https://orcid.org/0000-0003-1411-7624","observed_orcids":["https://orcid.org/0000-0003-1411-7624"]},"institutions":[{"id":"https://openalex.org/I86501945","display_name":"University of Delaware","ror":"https://ror.org/01sbq1a82","country_code":"US","type":"education","lineage":["https://openalex.org/I86501945"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Karl R. Schmitz","raw_affiliation_strings":["Department of Biological Sciences, University of Delaware, Newark DE, 19716","Department of Chemistry & Biochemistry, University of Delaware, Newark DE, 19716","Department of Biological Sciences, University of Delaware, Newark, DE, United States","Department of Chemistry and Biochemistry, University of Delaware, Newark, DE, United States"],"raw_orcid":"https://orcid.org/0000-0003-1411-7624","affiliations":[{"raw_affiliation_string":"Department of Biological Sciences, University of Delaware, Newark DE, 19716","institution_ids":["https://openalex.org/I86501945"]},{"raw_affiliation_string":"Department of Chemistry & Biochemistry, University of Delaware, Newark DE, 19716","institution_ids":["https://openalex.org/I86501945"]},{"raw_affiliation_string":"Department of Biological Sciences, University of Delaware, Newark, DE, United States","institution_ids":["https://openalex.org/I86501945"]},{"raw_affiliation_string":"Department of Chemistry and Biochemistry, University of Delaware, Newark, DE, United States","institution_ids":["https://openalex.org/I86501945"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5079741827"],"corresponding_institution_ids":["https://openalex.org/I86501945"],"apc_list":{"value":3295,"currency":"USD","value_usd":3295},"apc_paid":{"value":3295,"currency":"USD","value_usd":3295},"fwci":0.2665,"has_fulltext":false,"cited_by_count":3,"citation_normalized_percentile":{"value":0.42159426,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":96},"biblio":{"volume":"14","issue":null,"first_page":"1161764","last_page":"1161764"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10934","display_name":"Heat shock proteins research","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10934","display_name":"Heat shock proteins research","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/actinobacteria","display_name":"actinobacteria","score":0.9359999895095825},{"id":"https://openalex.org/keywords/clpb","display_name":"ClpB","score":0.8539999723434448},{"id":"https://openalex.org/keywords/clpc","display_name":"ClpC","score":1.0},{"id":"https://openalex.org/keywords/protein-quality-control","display_name":"protein quality control","score":0.9290000200271606},{"id":"https://openalex.org/keywords/m-domain","display_name":"M-domain","score":0.6010000109672546},{"id":"https://openalex.org/keywords/protein-disaggregation","display_name":"protein disaggregation","score":0.5049999952316284}],"concepts":[{"id":"https://openalex.org/C2777174735","wikidata":"https://www.wikidata.org/wiki/Q18047040","display_name":"CLPB","level":4,"score":0.9549918174743652},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7069482803344727},{"id":"https://openalex.org/C101635128","wikidata":"https://www.wikidata.org/wiki/Q4649768","display_name":"AAA proteins","level":4,"score":0.6199014782905579},{"id":"https://openalex.org/C2775962898","wikidata":"https://www.wikidata.org/wiki/Q5073152","display_name":"Chaperone (clinical)","level":2,"score":0.5289547443389893},{"id":"https://openalex.org/C2781307694","wikidata":"https://www.wikidata.org/wiki/Q33123","display_name":"Proteolysis","level":3,"score":0.5093098878860474},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4833618998527527},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.4470601975917816},{"id":"https://openalex.org/C199216141","wikidata":"https://www.wikidata.org/wiki/Q4995178","display_name":"Conserved sequence","level":4,"score":0.4407280683517456},{"id":"https://openalex.org/C144292202","wikidata":"https://www.wikidata.org/wiki/Q898273","display_name":"Protein domain","level":3,"score":0.4292256236076355},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.42851200699806213},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.38439589738845825},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3210010826587677},{"id":"https://openalex.org/C205260736","wikidata":"https://www.wikidata.org/wiki/Q418939","display_name":"Heat shock protein","level":3,"score":0.2938339114189148},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.2779676914215088},{"id":"https://openalex.org/C23265538","wikidata":"https://www.wikidata.org/wiki/Q300033","display_name":"ATPase","level":3,"score":0.27499526739120483},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.0},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.0}],"mesh":[],"locations_count":5,"locations":[{"id":"doi:10.3389/fmicb.2023.1161764","doi":"https://doi.org/10.3389/fmicb.2023.1161764","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fmicb.2023.1161764","pdf_url":null,"source":{"id":"https://openalex.org/S2484704948","display_name":"Frontiers in Microbiology","issn_l":"1664-302X","issn":["1664-302X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Microbiology","raw_type":"journal-article"},{"id":"doi:10.1101/2023.02.02.526855","doi":"https://doi.org/10.1101/2023.02.02.526855","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2023.02.02.526855","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2023/02/03/2023.02.02.526855.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},{"id":"pmh:oai:pubmedcentral.nih.gov:10149685","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/10149685","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Front Microbiol","raw_type":"Text"},{"id":"pmid:37138635","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/37138635","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in microbiology","raw_type":"Journal Article"},{"id":"pmh:oai:doaj.org/article:b8a87862d92e4021a9a5a7519363aa3a","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/b8a87862d92e4021a9a5a7519363aa3a","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Frontiers in Microbiology, Vol 14 (2023)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.3389/fmicb.2023.1161764","doi":"https://doi.org/10.3389/fmicb.2023.1161764","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fmicb.2023.1161764","pdf_url":null,"source":{"id":"https://openalex.org/S2484704948","display_name":"Frontiers in Microbiology","issn_l":"1664-302X","issn":["1664-302X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Microbiology","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G3037964080","display_name":"Discovery of Chemical Probes and Therapeutic Leads, Phase II","funder_award_id":"P20GM104316","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":80,"referenced_works":["https://openalex.org/W1045601090","https://openalex.org/W1571361882","https://openalex.org/W1595808396","https://openalex.org/W1650763821","https://openalex.org/W1857769113","https://openalex.org/W1963434023","https://openalex.org/W1969051510","https://openalex.org/W1973070864","https://openalex.org/W1974735434","https://openalex.org/W1976436656","https://openalex.org/W1993038297","https://openalex.org/W1997978648","https://openalex.org/W1998018574","https://openalex.org/W2009969297","https://openalex.org/W2011301426","https://openalex.org/W2012195402","https://openalex.org/W2012731700","https://openalex.org/W2038363407","https://openalex.org/W2045857220","https://openalex.org/W2052425412","https://openalex.org/W2055043387","https://openalex.org/W2055544854","https://openalex.org/W2061093994","https://openalex.org/W2064744296","https://openalex.org/W2065146040","https://openalex.org/W2069826477","https://openalex.org/W2070518402","https://openalex.org/W2077885055","https://openalex.org/W2093711359","https://openalex.org/W2106199951","https://openalex.org/W2106882534","https://openalex.org/W2109239634","https://openalex.org/W2110437187","https://openalex.org/W2114850508","https://openalex.org/W2117195560","https://openalex.org/W2132242403","https://openalex.org/W2132926880","https://openalex.org/W2135621733","https://openalex.org/W2135745367","https://openalex.org/W2143210482","https://openalex.org/W2146731040","https://openalex.org/W2150013803","https://openalex.org/W2150444353","https://openalex.org/W2151427055","https://openalex.org/W2158266834","https://openalex.org/W2168621448","https://openalex.org/W2242464395","https://openalex.org/W2277953252","https://openalex.org/W2302275788","https://openalex.org/W2320129280","https://openalex.org/W2376573086","https://openalex.org/W2403652171","https://openalex.org/W2528431093","https://openalex.org/W2740741280","https://openalex.org/W2767979133","https://openalex.org/W2797321272","https://openalex.org/W2806413543","https://openalex.org/W2893067023","https://openalex.org/W2893535877","https://openalex.org/W2907222071","https://openalex.org/W2911652560","https://openalex.org/W2913898479","https://openalex.org/W2951486119","https://openalex.org/W2998781879","https://openalex.org/W3006871946","https://openalex.org/W3008938277","https://openalex.org/W3016478508","https://openalex.org/W3016840971","https://openalex.org/W3019471649","https://openalex.org/W3023058184","https://openalex.org/W3025919310","https://openalex.org/W3045835921","https://openalex.org/W3133343543","https://openalex.org/W3156010855","https://openalex.org/W3177828909","https://openalex.org/W4283516015","https://openalex.org/W4302286504","https://openalex.org/W4304005546","https://openalex.org/W4308099559","https://openalex.org/W4313311869"],"related_works":["https://openalex.org/W2052425412","https://openalex.org/W3025919310","https://openalex.org/W2029020949","https://openalex.org/W2767979133","https://openalex.org/W2037454127","https://openalex.org/W1564936970","https://openalex.org/W2781010421","https://openalex.org/W1571361882","https://openalex.org/W7166736640","https://openalex.org/W2132242403"],"abstract_inverted_index":{"All":[0],"clades":[1],"of":[2,13,43,72,111,125,153,174],"bacteria":[3,158],"possess":[4,103,136],"Hsp100/Clp":[5],"family":[6],"unfoldase":[7,53],"enzymes":[8,81,155],"that":[9,110,134],"contribute":[10],"to":[11,38,49,85,109],"aspects":[12],"protein":[14,166],"quality":[15,167],"control.":[16],"In":[17,62],"Actinomycetota,":[18],"these":[19],"include":[20],"ClpB,":[21],"which":[22,32,76],"functions":[23],"as":[24],"an":[25,104],"independent":[26],"chaperone":[27],"and":[28,30,59,87,93,99,162,176],"disaggregase,":[29],"ClpC,":[31,88,112],"cooperates":[33],"with":[34,89,96,145,159],"the":[35,63,120,139,171],"ClpP1P2":[36],"peptidase":[37],"carry":[39],"out":[40],"regulated":[41],"proteolysis":[42],"client":[44],"proteins.":[45],"We":[46],"initially":[47],"sought":[48],"algorithmically":[50],"catalog":[51],"Clp":[52,74],"orthologs":[54],"from":[55],"Actinomycetota":[56],"into":[57,132],"ClpB":[58,86,175],"ClpC":[60],"categories.":[61],"process,":[64],"we":[65,77],"uncovered":[66],"a":[67],"phylogenetically":[68],"distinct":[69,148],"third":[70],"group":[71],"double-ringed":[73],"enzymes,":[75],"term":[78],"ClpI.":[79],"ClpI":[80,102,128,154],"are":[82,130],"architecturally":[83],"similar":[84,106],"intact":[90],"ATPase":[91],"modules":[92],"motifs":[94],"associated":[95],"substrate":[97],"unfolding":[98],"translation.":[100],"While":[101],"M-domain":[105],"in":[107],"length":[108],"its":[113],"N-terminal":[114,123],"domain":[115,124],"is":[116],"more":[117],"variable":[118],"than":[119],"strongly":[121],"conserved":[122,172],"ClpC.":[126,177],"Surprisingly,":[127],"sequences":[129],"divisible":[131],"sub-classes":[133],"either":[135],"or":[137],"lack":[138],"LGF-motifs":[140],"required":[141],"for":[142],"stable":[143],"assembly":[144],"ClpP1P2,":[146],"suggesting":[147],"cellular":[149],"roles.":[150],"The":[151],"presence":[152],"likely":[156],"provides":[157],"expanded":[160],"complexity":[161],"regulatory":[163],"control":[164,168],"over":[165],"programs,":[169],"supplementing":[170],"roles":[173]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2024,"cited_by_count":2}],"updated_date":"2026-10-29T09:56:32.521200","created_date":"2023-04-19T00:00:00"}