{"id":"https://openalex.org/W4362559909","doi":"https://doi.org/10.1002/edn3.408","title":"Evaluation of genetic diversity in an endangered fish Gnathopogon caerulescens using environmental DNA and its potential use in fish conservation","display_name":"Evaluation of genetic diversity in an endangered fish Gnathopogon caerulescens using environmental DNA and its potential use in fish conservation","publication_year":2023,"publication_date":"2023-04-03","ids":{"openalex":"https://openalex.org/W4362559909","doi":"https://doi.org/10.1002/edn3.408"},"language":"en","primary_location":{"id":"doi:10.1002/edn3.408","doi":"https://doi.org/10.1002/edn3.408","is_oa":true,"landing_page_url":"https://doi.org/10.1002/edn3.408","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/edn3.408","source":{"id":"https://openalex.org/S4210176772","display_name":"Environmental DNA","issn_l":"2637-4943","issn":["2637-4943"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-d","jufo-1","norway-1","poland-20","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Environmental DNA","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/edn3.408","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5085829402","display_name":"Kei Wakimura","orcid":"https://orcid.org/0000-0001-8052-4573","observed_orcids":["https://orcid.org/0000-0001-8052-4573"]},"institutions":[{"id":"https://openalex.org/I167598709","display_name":"Osaka Ohtani University","ror":"https://ror.org/01jtn9895","country_code":"JP","type":"education","lineage":["https://openalex.org/I167598709"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Kei Wakimura","raw_affiliation_strings":["Faculty of Pharmacy, Osaka Ohtani University, Tondabayashi, Japan"],"raw_orcid":"https://orcid.org/0000-0001-8052-4573","affiliations":[{"raw_affiliation_string":"Faculty of Pharmacy, Osaka Ohtani University, Tondabayashi, Japan","institution_ids":["https://openalex.org/I167598709"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5007407783","display_name":"Kimiko Uchii","orcid":"https://orcid.org/0000-0002-1375-3875","observed_orcids":["https://orcid.org/0000-0002-1375-3875"]},"institutions":[{"id":"https://openalex.org/I167598709","display_name":"Osaka Ohtani University","ror":"https://ror.org/01jtn9895","country_code":"JP","type":"education","lineage":["https://openalex.org/I167598709"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Kimiko Uchii","raw_affiliation_strings":["Faculty of Pharmacy, Osaka Ohtani University, Tondabayashi, Japan"],"raw_orcid":"https://orcid.org/0000-0002-1375-3875","affiliations":[{"raw_affiliation_string":"Faculty of Pharmacy, Osaka Ohtani University, Tondabayashi, Japan","institution_ids":["https://openalex.org/I167598709"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5091074265","display_name":"Takeshi Kikko","orcid":"https://orcid.org/0000-0003-3156-9374","observed_orcids":["https://orcid.org/0000-0003-3156-9374","https://orcid.org/0000-0002-7009-7480"]},"institutions":[{"id":"https://openalex.org/I916559398","display_name":"Kindai University","ror":"https://ror.org/05kt9ap64","country_code":"JP","type":"education","lineage":["https://openalex.org/I916559398"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Takeshi Kikko","raw_affiliation_strings":["Faculty of Agriculture, Kindai University, Nara, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Faculty of Agriculture, Kindai University, Nara, Japan","institution_ids":["https://openalex.org/I916559398"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5007407783"],"corresponding_institution_ids":["https://openalex.org/I167598709"],"apc_list":{"value":2750,"currency":"USD","value_usd":2750},"apc_paid":{"value":2750,"currency":"USD","value_usd":2750},"fwci":1.8217,"has_fulltext":false,"cited_by_count":15,"citation_normalized_percentile":{"value":0.85567938,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":99},"biblio":{"volume":"5","issue":"5","first_page":"973","last_page":"986"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10012","display_name":"Genetic diversity and population structure","score":0.9452999830245972,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10012","display_name":"Genetic diversity and population structure","score":0.9452999830245972,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12640","display_name":"Environmental DNA in Biodiversity Studies","score":0.04340000078082085,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10751","display_name":"Forensic and Genetic Research","score":0.003599999938160181,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/gnathopogon-caerulescens","display_name":"Gnathopogon caerulescens","score":1.0},{"id":"https://openalex.org/keywords/genetic-diversity","display_name":"genetic diversity","score":0.8989999890327454},{"id":"https://openalex.org/keywords/mitochondrial-haplotypes","display_name":"mitochondrial haplotypes","score":0.9160000085830688},{"id":"https://openalex.org/keywords/endangered-fish","display_name":"endangered fish","score":0.625},{"id":"https://openalex.org/keywords/fish-conservation","display_name":"fish conservation","score":0.7950000166893005},{"id":"https://openalex.org/keywords/population-structure","display_name":"population structure","score":0.7509999871253967},{"id":"https://openalex.org/keywords/haplotype-network","display_name":"haplotype network","score":0.8730000257492065},{"id":"https://openalex.org/keywords/population-decline","display_name":"population decline","score":0.8270000219345093},{"id":"https://openalex.org/keywords/lake-biwa","display_name":"Lake Biwa","score":0.8519999980926514}],"concepts":[{"id":"https://openalex.org/C179345059","wikidata":"https://www.wikidata.org/wiki/Q11394","display_name":"Endangered species","level":3,"score":0.8287089467048645},{"id":"https://openalex.org/C197754878","wikidata":"https://www.wikidata.org/wiki/Q80711","display_name":"Haplotype","level":4,"score":0.6703834533691406},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6586197018623352},{"id":"https://openalex.org/C81977670","wikidata":"https://www.wikidata.org/wiki/Q585259","display_name":"Genetic diversity","level":3,"score":0.6202564239501953},{"id":"https://openalex.org/C2908647359","wikidata":"https://www.wikidata.org/wiki/Q2625603","display_name":"Population","level":2,"score":0.6181971430778503},{"id":"https://openalex.org/C51213014","wikidata":"https://www.wikidata.org/wiki/Q4390843","display_name":"Conservation genetics","level":5,"score":0.5786674618721008},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.4746164381504059},{"id":"https://openalex.org/C78458016","wikidata":"https://www.wikidata.org/wiki/Q840400","display_name":"Evolutionary biology","level":1,"score":0.38905611634254456},{"id":"https://openalex.org/C505870484","wikidata":"https://www.wikidata.org/wiki/Q180538","display_name":"Fishery","level":1,"score":0.32533109188079834},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.19170185923576355},{"id":"https://openalex.org/C61320498","wikidata":"https://www.wikidata.org/wiki/Q265193","display_name":"Microsatellite","level":4,"score":0.17250338196754456},{"id":"https://openalex.org/C180754005","wikidata":"https://www.wikidata.org/wiki/Q80726","display_name":"Allele","level":3,"score":0.12409195303916931},{"id":"https://openalex.org/C149923435","wikidata":"https://www.wikidata.org/wiki/Q37732","display_name":"Demography","level":1,"score":0.07667091488838196},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.05909892916679382},{"id":"https://openalex.org/C144024400","wikidata":"https://www.wikidata.org/wiki/Q21201","display_name":"Sociology","level":0,"score":0.0},{"id":"https://openalex.org/C185933670","wikidata":"https://www.wikidata.org/wiki/Q52105","display_name":"Habitat","level":2,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1002/edn3.408","doi":"https://doi.org/10.1002/edn3.408","is_oa":true,"landing_page_url":"https://doi.org/10.1002/edn3.408","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/edn3.408","source":{"id":"https://openalex.org/S4210176772","display_name":"Environmental DNA","issn_l":"2637-4943","issn":["2637-4943"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-d","jufo-1","norway-1","poland-20","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Environmental DNA","raw_type":"journal-article"},{"id":"pmh:oai:doaj.org/article:6c5703b0207041b78abb3c57b854d4ee","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/6c5703b0207041b78abb3c57b854d4ee","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Environmental DNA, Vol 5, Iss 5, Pp 973-986 (2023)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1002/edn3.408","doi":"https://doi.org/10.1002/edn3.408","is_oa":true,"landing_page_url":"https://doi.org/10.1002/edn3.408","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/edn3.408","source":{"id":"https://openalex.org/S4210176772","display_name":"Environmental DNA","issn_l":"2637-4943","issn":["2637-4943"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-d","jufo-1","norway-1","poland-20","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Environmental DNA","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Life on land","score":0.7605999708175659,"id":"https://metadata.un.org/sdg/15"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G4918538456","display_name":"Advancing environmental DNA analysis through the development of nuclear DNA markers and long-read sequencing approaches","funder_award_id":"23K21776","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"},{"id":"https://openalex.org/G6290141934","display_name":null,"funder_award_id":"21H03654","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"},{"id":"https://openalex.org/G8728393551","display_name":null,"funder_award_id":"JPMEERF20204004","funder_id":"https://openalex.org/F4320315051","funder_display_name":"Environmental Restoration and Conservation Agency"}],"funders":[{"id":"https://openalex.org/F4320315051","display_name":"Environmental Restoration and Conservation Agency","ror":"https://ror.org/01c9agy38"},{"id":"https://openalex.org/F4320334764","display_name":"Japan Society for the Promotion of Science","ror":"https://ror.org/00hhkn466"}],"has_content":{"grobid_xml":false,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4362559909.pdf"},"referenced_works_count":68,"referenced_works":["https://openalex.org/W631555623","https://openalex.org/W1633528265","https://openalex.org/W1768287364","https://openalex.org/W1984390786","https://openalex.org/W1990074132","https://openalex.org/W2009783955","https://openalex.org/W2029580191","https://openalex.org/W2035164312","https://openalex.org/W2063580733","https://openalex.org/W2084787000","https://openalex.org/W2092512759","https://openalex.org/W2094057382","https://openalex.org/W2100444509","https://openalex.org/W2119742475","https://openalex.org/W2122606427","https://openalex.org/W2126753786","https://openalex.org/W2131176533","https://openalex.org/W2133717069","https://openalex.org/W2135598075","https://openalex.org/W2136489776","https://openalex.org/W2138832938","https://openalex.org/W2140784970","https://openalex.org/W2155136887","https://openalex.org/W2168560149","https://openalex.org/W2171783890","https://openalex.org/W2231695592","https://openalex.org/W2401404581","https://openalex.org/W2460850757","https://openalex.org/W2497892257","https://openalex.org/W2538509698","https://openalex.org/W2553951889","https://openalex.org/W2555113199","https://openalex.org/W2559471126","https://openalex.org/W2569995699","https://openalex.org/W2594312091","https://openalex.org/W2760087951","https://openalex.org/W2900997220","https://openalex.org/W2911813797","https://openalex.org/W2913725860","https://openalex.org/W2917802047","https://openalex.org/W2936258349","https://openalex.org/W2947595158","https://openalex.org/W2953063653","https://openalex.org/W2956439456","https://openalex.org/W2980767418","https://openalex.org/W2981169658","https://openalex.org/W2981728262","https://openalex.org/W2999369378","https://openalex.org/W3024579709","https://openalex.org/W3089619765","https://openalex.org/W3094136461","https://openalex.org/W3102121856","https://openalex.org/W3112596155","https://openalex.org/W3118726828","https://openalex.org/W3132644980","https://openalex.org/W3133304149","https://openalex.org/W3148604764","https://openalex.org/W3155691078","https://openalex.org/W3158182418","https://openalex.org/W3166888189","https://openalex.org/W3174164385","https://openalex.org/W3175417001","https://openalex.org/W3188721201","https://openalex.org/W4210894766","https://openalex.org/W4233131144","https://openalex.org/W4254687493","https://openalex.org/W4286492133","https://openalex.org/W4295013933"],"related_works":["https://openalex.org/W3188721201","https://openalex.org/W2231695592","https://openalex.org/W4295013933","https://openalex.org/W2893465925","https://openalex.org/W3029818532","https://openalex.org/W3015995332","https://openalex.org/W4411329176","https://openalex.org/W2531415679","https://openalex.org/W4308349415","https://openalex.org/W2137111518"],"abstract_inverted_index":{"Abstract":[0],"Gnathopogon":[1],"caerulescens,":[2],"an":[3,25,86],"endemic":[4],"fish":[5,251],"of":[6,12,42,50,69,81,128,202,230,236,250],"Lake":[7],"Biwa,":[8],"Japan,":[9],"is":[10],"one":[11],"the":[13,33,48,64,78,105,120,125,156,173,181,185,191,197,211,219,223,227,246],"most":[14],"important":[15],"fishes":[16],"for":[17,54,147],"lake":[18],"fisheries.":[19],"It":[20],"has":[21],"been":[22],"listed":[23],"as":[24],"endangered":[26,237],"species":[27],"following":[28],"its":[29,166],"population":[30,159,167,182,214,234],"decline":[31,183,212],"in":[32,184,196,200,213,218,222,233],"1990s":[34],"and":[35,98,165,205,248],"thus":[36],"needs":[37],"conservation.":[38],"An":[39],"increasing":[40],"number":[41,204],"studies":[43],"have":[44],"recently":[45],"stressed":[46],"on":[47],"importance":[49],"incorporating":[51],"genetic":[52,79,126,192],"diversity":[53,80,193,207],"conservation":[55,247],"purposes.":[56],"In":[57],"this":[58,82,242],"study,":[59],"we":[60],"aimed":[61],"to":[62,76,172],"evaluate":[63],"mitochondrial":[65],"DNA":[66,74],"haplotype":[67,136,203,206],"composition":[68],"G.":[70,130,157],"caerulescens":[71,131,158],"using":[72,90,119,241],"environmental":[73],"(eDNA)":[75],"determine":[77],"species.":[83,238],"We":[84,133,187,225],"developed":[85,121],"eDNA\u2010based":[87],"haplotyping":[88],"approach":[89,243],"high\u2010throughput":[91],"sequencing":[92],"(HTS),":[93],"which":[94,153],"can":[95],"accurately":[96],"detect":[97],"eliminate":[99],"erroneous":[100],"HTS":[101],"reads,":[102],"even":[103],"when":[104],"target":[106],"sequences":[107],"are":[108],"differentiated":[109],"by":[110,143],"a":[111,114,162],"single":[112],"or":[113],"few":[115],"mismatches.":[116],"Field":[117],"surveys":[118],"method":[122],"successfully":[123],"revealed":[124],"structure":[127],"local":[129,149],"populations.":[132],"found":[134],"star\u2010shaped":[135],"networks":[137],"with":[138],"two":[139],"major":[140],"haplotypes":[141,146,177],"accompanied":[142],"several":[144],"rare":[145,176],"all":[148],"populations":[150,199],"except":[151],"one,":[152],"suggested":[154],"that":[155,175,190,210],"had":[160],"undergone":[161],"historical":[163],"expansion,":[164],"size":[168],"did":[169],"not":[170],"shrink":[171],"extent":[174],"were":[178],"eliminated":[179],"during":[180],"1990s.":[186],"also":[188],"observed":[189],"was":[194,215],"lower":[195],"northern":[198],"terms":[201],"(h),":[208],"suggesting":[209],"more":[216],"severe":[217],"north":[220],"than":[221],"south.":[224],"demonstrated":[226],"practical":[228],"use":[229],"eDNA":[231],"analysis":[232],"genetics":[235],"Continuous":[239],"monitoring":[240],"would":[244],"facilitate":[245],"management":[249],"resources.":[252]},"counts_by_year":[{"year":2026,"cited_by_count":6},{"year":2025,"cited_by_count":4},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":2}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2023-04-06T00:00:00"}
