{"id":"https://openalex.org/W4323314288","doi":"https://doi.org/10.3390/ijms24054945","title":"Rapid Generation of Barley Homozygous Transgenic Lines Based on Microspore Culture: HvPR1 Overexpression as an Example","display_name":"Rapid Generation of Barley Homozygous Transgenic Lines Based on Microspore Culture: HvPR1 Overexpression as an Example","publication_year":2023,"publication_date":"2023-03-03","ids":{"openalex":"https://openalex.org/W4323314288","doi":"https://doi.org/10.3390/ijms24054945","pmid":"https://pubmed.ncbi.nlm.nih.gov/36902374","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/10003194"},"language":"en","primary_location":{"id":"doi:10.3390/ijms24054945","doi":"https://doi.org/10.3390/ijms24054945","is_oa":true,"landing_page_url":"https://doi.org/10.3390/ijms24054945","pdf_url":"https://www.mdpi.com/1422-0067/24/5/4945/pdf?version=1678196833","source":{"id":"https://openalex.org/S10623703","display_name":"International Journal of Molecular Sciences","issn_l":"1422-0067","issn":["1422-0067","1661-6596"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","medline","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International Journal of Molecular Sciences","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.mdpi.com/1422-0067/24/5/4945/pdf?version=1678196833","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101895731","display_name":"Zhiwei Chen","orcid":"https://orcid.org/0000-0002-0478-4880","observed_orcids":["https://orcid.org/0000-0002-0478-4880"]},"institutions":[{"id":"https://openalex.org/I4210139732","display_name":"Shanghai Academy of Agricultural Sciences","ror":"https://ror.org/04ejmmq75","country_code":"CN","type":"facility","lineage":["https://openalex.org/I4210139732"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Zhiwei Chen","raw_affiliation_strings":["Shanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China"],"raw_orcid":"https://orcid.org/0000-0002-0478-4880","affiliations":[{"raw_affiliation_string":"Shanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China","institution_ids":["https://openalex.org/I4210139732"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068270023","display_name":"Qi Jiang","orcid":"https://orcid.org/0000-0002-2187-5856","observed_orcids":["https://orcid.org/0000-0002-2187-5856"]},"institutions":[{"id":"https://openalex.org/I4210139732","display_name":"Shanghai Academy of Agricultural Sciences","ror":"https://ror.org/04ejmmq75","country_code":"CN","type":"facility","lineage":["https://openalex.org/I4210139732"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Qi Jiang","raw_affiliation_strings":["Shanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Shanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China","institution_ids":["https://openalex.org/I4210139732"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102360858","display_name":"Guimei Guo","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210139732","display_name":"Shanghai Academy of Agricultural Sciences","ror":"https://ror.org/04ejmmq75","country_code":"CN","type":"facility","lineage":["https://openalex.org/I4210139732"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Guimei Guo","raw_affiliation_strings":["Shanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Shanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China","institution_ids":["https://openalex.org/I4210139732"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5049285098","display_name":"Qiufang Shen","orcid":"https://orcid.org/0000-0002-6589-7379","observed_orcids":["https://orcid.org/0000-0002-6589-7379"]},"institutions":[{"id":"https://openalex.org/I76130692","display_name":"Zhejiang University","ror":"https://ror.org/00a2xv884","country_code":"CN","type":"education","lineage":["https://openalex.org/I76130692"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Qiufang Shen","raw_affiliation_strings":["Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China"],"raw_orcid":"https://orcid.org/0000-0002-6589-7379","affiliations":[{"raw_affiliation_string":"Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China","institution_ids":["https://openalex.org/I76130692"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5032178109","display_name":"Jun Yang","orcid":"https://orcid.org/0000-0002-4527-3219","observed_orcids":["https://orcid.org/0000-0002-4527-3219"]},"institutions":[{"id":"https://openalex.org/I19820366","display_name":"Chinese Academy of Sciences","ror":"https://ror.org/034t30j35","country_code":"CN","type":"government","lineage":["https://openalex.org/I19820366"]},{"id":"https://openalex.org/I4210106477","display_name":"Shanghai Institutes for Biological Sciences","ror":"https://ror.org/011rf9d86","country_code":"CN","type":"facility","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210106477"]},{"id":"https://openalex.org/I4210139738","display_name":"Center for Excellence in Molecular Plant Sciences","ror":"https://ror.org/04ew43640","country_code":"CN","type":"education","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210139738"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Jun Yang","raw_affiliation_strings":["National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, SIBS, Chinese Academy of Sciences, Shanghai 200032, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, SIBS, Chinese Academy of Sciences, Shanghai 200032, China","institution_ids":["https://openalex.org/I19820366","https://openalex.org/I4210106477","https://openalex.org/I4210139738"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5020878352","display_name":"Ertao Wang","orcid":"https://orcid.org/0000-0002-5178-3530","observed_orcids":["https://orcid.org/0000-0002-5178-3530","https://orcid.org/0000-0003-3978-0828"]},"institutions":[{"id":"https://openalex.org/I19820366","display_name":"Chinese Academy of Sciences","ror":"https://ror.org/034t30j35","country_code":"CN","type":"government","lineage":["https://openalex.org/I19820366"]},{"id":"https://openalex.org/I4210106477","display_name":"Shanghai Institutes for Biological Sciences","ror":"https://ror.org/011rf9d86","country_code":"CN","type":"facility","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210106477"]},{"id":"https://openalex.org/I4210139738","display_name":"Center for Excellence in Molecular Plant Sciences","ror":"https://ror.org/04ew43640","country_code":"CN","type":"education","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210139738"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Ertao Wang","raw_affiliation_strings":["National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, SIBS, Chinese Academy of Sciences, Shanghai 200032, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, SIBS, Chinese Academy of Sciences, Shanghai 200032, China","institution_ids":["https://openalex.org/I19820366","https://openalex.org/I4210106477","https://openalex.org/I4210139738"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100753746","display_name":"Guoping Zhang","orcid":"https://orcid.org/0000-0002-9042-2607","observed_orcids":["https://orcid.org/0000-0002-9042-2607"]},"institutions":[{"id":"https://openalex.org/I76130692","display_name":"Zhejiang University","ror":"https://ror.org/00a2xv884","country_code":"CN","type":"education","lineage":["https://openalex.org/I76130692"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Guoping Zhang","raw_affiliation_strings":["Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China","institution_ids":["https://openalex.org/I76130692"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5048765393","display_name":"Ruiju Lu","orcid":"https://orcid.org/0000-0003-1760-3791","observed_orcids":["https://orcid.org/0000-0003-1760-3791"]},"institutions":[{"id":"https://openalex.org/I4210139732","display_name":"Shanghai Academy of Agricultural Sciences","ror":"https://ror.org/04ejmmq75","country_code":"CN","type":"facility","lineage":["https://openalex.org/I4210139732"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Ruiju Lu","raw_affiliation_strings":["Shanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Shanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China","institution_ids":["https://openalex.org/I4210139732"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5043776369","display_name":"Chenghong Liu","orcid":"https://orcid.org/0000-0002-3556-5648","observed_orcids":["https://orcid.org/0000-0002-3556-5648","https://orcid.org/0009-0002-3009-4127"]},"institutions":[{"id":"https://openalex.org/I4210139732","display_name":"Shanghai Academy of Agricultural Sciences","ror":"https://ror.org/04ejmmq75","country_code":"CN","type":"facility","lineage":["https://openalex.org/I4210139732"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Chenghong Liu","raw_affiliation_strings":["Shanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Shanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China","institution_ids":["https://openalex.org/I4210139732"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":5,"corresponding_author_ids":["https://openalex.org/A5043776369","https://openalex.org/A5101895731"],"corresponding_institution_ids":["https://openalex.org/I4210139732"],"apc_list":{"value":2500,"currency":"CHF","value_usd":2780},"apc_paid":{"value":2500,"currency":"CHF","value_usd":2780},"fwci":0.6924,"has_fulltext":true,"cited_by_count":5,"citation_normalized_percentile":{"value":0.64138221,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"24","issue":"5","first_page":"4945","last_page":"4945"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":0.9854000210762024,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":0.9854000210762024,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10733","display_name":"Wheat and Barley Genetics and Pathology","score":0.007699999958276749,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11426","display_name":"Genetically Modified Organisms Research","score":0.0034000000450760126,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/barley","display_name":"barley","score":0.9710000157356262},{"id":"https://openalex.org/keywords/microspore-culture","display_name":"microspore culture","score":0.8799999952316284},{"id":"https://openalex.org/keywords/pr1","display_name":"PR1","score":0.9210000038146973},{"id":"https://openalex.org/keywords/gene-overexpression","display_name":"gene overexpression","score":0.949999988079071},{"id":"https://openalex.org/keywords/nitrogen-use-efficiency","display_name":"nitrogen use efficiency","score":0.9909999966621399}],"concepts":[{"id":"https://openalex.org/C121807389","wikidata":"https://www.wikidata.org/wiki/Q2452297","display_name":"Microspore","level":4,"score":0.9395111799240112},{"id":"https://openalex.org/C34827325","wikidata":"https://www.wikidata.org/wiki/Q3037604","display_name":"Doubled haploidy","level":4,"score":0.820145845413208},{"id":"https://openalex.org/C102230213","wikidata":"https://www.wikidata.org/wiki/Q430117","display_name":"Transgene","level":3,"score":0.7809740304946899},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7328471541404724},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5998145937919617},{"id":"https://openalex.org/C122172344","wikidata":"https://www.wikidata.org/wiki/Q1503477","display_name":"Genetically modified crops","level":4,"score":0.5437970161437988},{"id":"https://openalex.org/C127716648","wikidata":"https://www.wikidata.org/wiki/Q104053","display_name":"Phenotype","level":3,"score":0.5284112691879272},{"id":"https://openalex.org/C29172406","wikidata":"https://www.wikidata.org/wiki/Q103129","display_name":"Stamen","level":3,"score":0.5008544921875},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.4934760332107544},{"id":"https://openalex.org/C143191323","wikidata":"https://www.wikidata.org/wiki/Q118406","display_name":"Ploidy","level":3,"score":0.43171778321266174},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.33997848629951477},{"id":"https://openalex.org/C2780618852","wikidata":"https://www.wikidata.org/wiki/Q79932","display_name":"Pollen","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D001467","descriptor_name":"Hordeum","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D006238","descriptor_name":"Haploidy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006720","descriptor_name":"Homozygote","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010641","descriptor_name":"Phenotype","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.3390/ijms24054945","doi":"https://doi.org/10.3390/ijms24054945","is_oa":true,"landing_page_url":"https://doi.org/10.3390/ijms24054945","pdf_url":"https://www.mdpi.com/1422-0067/24/5/4945/pdf?version=1678196833","source":{"id":"https://openalex.org/S10623703","display_name":"International Journal of Molecular Sciences","issn_l":"1422-0067","issn":["1422-0067","1661-6596"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","medline","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International Journal of Molecular Sciences","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:10003194","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/10003194","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC10003194/pdf/ijms-24-04945.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Int J Mol Sci","raw_type":"Text"},{"id":"pmid:36902374","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/36902374","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International journal of molecular sciences","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.3390/ijms24054945","doi":"https://doi.org/10.3390/ijms24054945","is_oa":true,"landing_page_url":"https://doi.org/10.3390/ijms24054945","pdf_url":"https://www.mdpi.com/1422-0067/24/5/4945/pdf?version=1678196833","source":{"id":"https://openalex.org/S10623703","display_name":"International Journal of Molecular Sciences","issn_l":"1422-0067","issn":["1422-0067","1661-6596"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","medline","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International Journal of Molecular Sciences","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Zero hunger","score":0.9208999872207642,"id":"https://metadata.un.org/sdg/2"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1654588718","display_name":null,"funder_award_id":"BB/ P016855/1","funder_id":"https://openalex.org/F4320334629","funder_display_name":"Biotechnology and Biological Sciences Research Council"},{"id":"https://openalex.org/G2439093589","display_name":null,"funder_award_id":"PG22211","funder_id":"https://openalex.org/F4320309612","funder_display_name":"Natural Science Foundation of Shanghai"},{"id":"https://openalex.org/G3821851789","display_name":null,"funder_award_id":"2018YFD1000702-5","funder_id":"https://openalex.org/F4320335777","funder_display_name":"National Key Research and Development Program of China"},{"id":"https://openalex.org/G5877257233","display_name":null,"funder_award_id":"PG22211","funder_id":"https://openalex.org/F4320335777","funder_display_name":"National Key Research and Development Program of China"},{"id":"https://openalex.org/G6434906143","display_name":null,"funder_award_id":"CARS-05-01A-02","funder_id":"https://openalex.org/F4320309612","funder_display_name":"Natural Science Foundation of Shanghai"},{"id":"https://openalex.org/G6485083907","display_name":null,"funder_award_id":"2018YFD1000702-5","funder_id":"https://openalex.org/F4320309612","funder_display_name":"Natural Science Foundation of Shanghai"},{"id":"https://openalex.org/G6592243765","display_name":null,"funder_award_id":"CARS-05-01A-02","funder_id":"https://openalex.org/F4320335777","funder_display_name":"National Key Research and Development Program of China"},{"id":"https://openalex.org/G6748883917","display_name":null,"funder_award_id":"17ZR1425300","funder_id":"https://openalex.org/F4320335777","funder_display_name":"National Key Research and Development Program of China"},{"id":"https://openalex.org/G7540784902","display_name":null,"funder_award_id":"17ZR1425300","funder_id":"https://openalex.org/F4320309612","funder_display_name":"Natural Science Foundation of Shanghai"},{"id":"https://openalex.org/G8839311157","display_name":null,"funder_award_id":"PG22211","funder_id":"https://openalex.org/F4320324227","funder_display_name":"Shanghai Academy of Agricultural Sciences"}],"funders":[{"id":"https://openalex.org/F4320309612","display_name":"Natural Science Foundation of Shanghai","ror":null},{"id":"https://openalex.org/F4320324227","display_name":"Shanghai Academy of Agricultural Sciences","ror":"https://ror.org/04ejmmq75"},{"id":"https://openalex.org/F4320325211","display_name":"Nanjing Agricultural University","ror":"https://ror.org/05td3s095"},{"id":"https://openalex.org/F4320334629","display_name":"Biotechnology and Biological Sciences Research Council","ror":"https://ror.org/00cwqg982"},{"id":"https://openalex.org/F4320335777","display_name":"National Key Research and Development Program of China","ror":null}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4323314288.pdf","grobid_xml":"https://content.openalex.org/works/W4323314288.grobid-xml"},"referenced_works_count":23,"referenced_works":["https://openalex.org/W1517984787","https://openalex.org/W1947422755","https://openalex.org/W1968296907","https://openalex.org/W1988902793","https://openalex.org/W2005134278","https://openalex.org/W2042359887","https://openalex.org/W2142553120","https://openalex.org/W2476212746","https://openalex.org/W2482602903","https://openalex.org/W2765492128","https://openalex.org/W2795863559","https://openalex.org/W2809229076","https://openalex.org/W2901056248","https://openalex.org/W2945224186","https://openalex.org/W2983912319","https://openalex.org/W3014155869","https://openalex.org/W3033394385","https://openalex.org/W3036166668","https://openalex.org/W3042739182","https://openalex.org/W3113071913","https://openalex.org/W3187965529","https://openalex.org/W4210709492","https://openalex.org/W4293242827"],"related_works":["https://openalex.org/W3042739182","https://openalex.org/W2047718451","https://openalex.org/W2349429513","https://openalex.org/W1885844047","https://openalex.org/W1967567916","https://openalex.org/W160522266","https://openalex.org/W1995667460","https://openalex.org/W2795863559","https://openalex.org/W2030734807","https://openalex.org/W2089526833"],"abstract_inverted_index":{"Obtaining":[0],"homozygous":[1,17,46,125],"lines":[2,127,135,151],"from":[3,56,96],"transgenic":[4,50,59,126,134],"plants":[5,18,51,94],"is":[6,19],"an":[7,144],"important":[8],"step":[9],"for":[10,136,156],"phenotypic":[11],"evaluations,":[12],"but":[13],"the":[14,62,68,84,97,106,130,146],"selection":[15],"of":[16,67,108,123,133,149,159],"time-consuming":[20],"and":[21,74,140],"laborious.":[22],"The":[23,120],"process":[24],"would":[25],"be":[26,35,154],"significantly":[27],"shortened":[28],"if":[29],"anther":[30],"or":[31],"microspore":[32,54],"culture":[33,55],"could":[34,153],"completed":[36],"in":[37,162],"one":[38,57],"generation.":[39],"In":[40],"this":[41],"study,":[42],"we":[43],"obtained":[44],"24":[45],"doubled":[47,69],"haploid":[48],"(DH)":[49],"entirely":[52],"by":[53],"T0":[58],"plant":[60],"overexpressing":[61],"gene":[63,86,137],"HvPR1":[64,85,109,147],"(pathogenesis-related-1).":[65],"Nine":[66],"haploids":[70],"grew":[71],"to":[72],"maturity":[73],"produced":[75],"seeds.":[76],"qRCR":[77],"(quantitative":[78],"real-time":[79],"PCR)":[80],"validation":[81],"showed":[82],"that":[83,105],"was":[87],"expressed":[88],"differentially":[89],"even":[90],"among":[91],"different":[92],"DH1":[93],"(T2)":[95],"same":[98],"DH0":[99],"line":[100],"(T1).":[101],"Phenotyping":[102],"analysis":[103,158],"suggested":[104],"overexpression":[107,148],"inhibited":[110],"nitrogen":[111,118],"use":[112],"efficiency":[113],"(NUE)":[114],"only":[115],"under":[116],"low":[117],"treatment.":[119],"established":[121],"method":[122],"producing":[124],"will":[128],"enable":[129],"rapid":[131],"evaluation":[132],"function":[138],"studies":[139],"trait":[141],"evaluation.":[142],"As":[143],"example,":[145],"DH":[150],"also":[152],"used":[155],"further":[157],"NUE-related":[160],"research":[161],"barley.":[163]},"counts_by_year":[{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2023-03-07T00:00:00"}