{"id":"https://openalex.org/W4322377609","doi":"https://doi.org/10.1093/clinchem/45.8.1141","title":"Rapid, Homogeneous Genotyping of the 4G/5G Polymorphism in the Promoter Region of the PAI1 Gene by Fluorescence Resonance Energy Transfer and Probe Melting Curves","display_name":"Rapid, Homogeneous Genotyping of the 4G/5G Polymorphism in the Promoter Region of the PAI1 Gene by Fluorescence Resonance Energy Transfer and Probe Melting Curves","publication_year":1999,"publication_date":"1999-08-01","ids":{"openalex":"https://openalex.org/W4322377609","doi":"https://doi.org/10.1093/clinchem/45.8.1141","mag":"1955929846","pmid":"https://pubmed.ncbi.nlm.nih.gov/10430777"},"language":"en","primary_location":{"id":"doi:10.1093/clinchem/45.8.1141","doi":"https://doi.org/10.1093/clinchem/45.8.1141","is_oa":false,"landing_page_url":"https://doi.org/10.1093/clinchem/45.8.1141","pdf_url":null,"source":{"id":"https://openalex.org/S139212996","display_name":"Clinical Chemistry","issn_l":"0009-9147","issn":["0009-9147","1530-8561"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320272","host_organization_name":"American Association for Clinical Chemistry","host_organization_lineage":["https://openalex.org/P4310320272"],"host_organization_lineage_names":["American Association for Clinical Chemistry"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Clinical Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5023899902","display_name":"Markus Nauck","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Markus Nauck","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5124605891","display_name":"Heinrich Wieland","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Heinrich Wieland","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5000046286","display_name":"Winfried M\u00e4rz","orcid":"https://orcid.org/0000-0001-6083-8946","observed_orcids":["https://orcid.org/0000-0001-6083-8946","https://orcid.org/0000-0002-5358-4463"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Winfried Ma\u0308rz","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5023899902"],"corresponding_institution_ids":[],"apc_list":{"value":4636,"currency":"USD","value_usd":4636},"apc_paid":null,"fwci":2.8354,"has_fulltext":false,"cited_by_count":37,"citation_normalized_percentile":{"value":0.91296502,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":95},"biblio":{"volume":"45","issue":"8","first_page":"1141","last_page":"1147"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10881","display_name":"Blood Coagulation and Thrombosis Mechanisms","score":0.513700008392334,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10881","display_name":"Blood Coagulation and Thrombosis Mechanisms","score":0.513700008392334,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.3700999915599823,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10261","display_name":"Genetic Associations and Epidemiology","score":0.0649000033736229,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/pai-1-gene","display_name":"PAI-1 gene","score":0.765999972820282},{"id":"https://openalex.org/keywords/plasminogen-activator-inhibitor-1","display_name":"plasminogen activator inhibitor-1","score":0.9259999990463257},{"id":"https://openalex.org/keywords/4g-5g-polymorphism","display_name":"4G/5G polymorphism","score":0.9629999995231628},{"id":"https://openalex.org/keywords/promoter-polymorphism","display_name":"promoter polymorphism","score":0.8610000014305115},{"id":"https://openalex.org/keywords/genotyping","display_name":"genotyping","score":0.6179999709129333},{"id":"https://openalex.org/keywords/fluorescence-resonance-energy-transfer","display_name":"fluorescence resonance energy transfer","score":0.847000002861023},{"id":"https://openalex.org/keywords/fibrinolysis","display_name":"fibrinolysis","score":0.8560000061988831},{"id":"https://openalex.org/keywords/myocardial-infarction","display_name":"myocardial infarction","score":0.9869999885559082}],"concepts":[{"id":"https://openalex.org/C31467283","wikidata":"https://www.wikidata.org/wiki/Q912147","display_name":"Genotyping","level":4,"score":0.8582339286804199},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.7034639120101929},{"id":"https://openalex.org/C84133368","wikidata":"https://www.wikidata.org/wiki/Q6813768","display_name":"Melting curve analysis","level":4,"score":0.6834945678710938},{"id":"https://openalex.org/C135763542","wikidata":"https://www.wikidata.org/wiki/Q106016","display_name":"Genotype","level":3,"score":0.6833916306495667},{"id":"https://openalex.org/C96305047","wikidata":"https://www.wikidata.org/wiki/Q367473","display_name":"F\u00f6rster resonance energy transfer","level":3,"score":0.6262003183364868},{"id":"https://openalex.org/C8185291","wikidata":"https://www.wikidata.org/wiki/Q2844457","display_name":"Amplicon","level":4,"score":0.5684789419174194},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5565052628517151},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.446749746799469},{"id":"https://openalex.org/C180754005","wikidata":"https://www.wikidata.org/wiki/Q80726","display_name":"Allele","level":3,"score":0.4465675354003906},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.41652312874794006},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.4022708237171173},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.27824053168296814},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000483","descriptor_name":"Alleles","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005456","descriptor_name":"Fluorescent Dyes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005838","descriptor_name":"Genotype","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011401","descriptor_name":"Promoter Regions, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012150","descriptor_name":"Polymorphism, Restriction Fragment Length","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013050","descriptor_name":"Spectrometry, Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016254","descriptor_name":"Mutagenesis, Insertional","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017384","descriptor_name":"Sequence Deletion","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017395","descriptor_name":"Plasminogen Activator Inhibitor 1","qualifier_ui":"Q000097","qualifier_name":"blood","is_major_topic":false},{"descriptor_ui":"D017395","descriptor_name":"Plasminogen Activator Inhibitor 1","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true}],"locations_count":5,"locations":[{"id":"doi:10.1093/clinchem/45.8.1141","doi":"https://doi.org/10.1093/clinchem/45.8.1141","is_oa":false,"landing_page_url":"https://doi.org/10.1093/clinchem/45.8.1141","pdf_url":null,"source":{"id":"https://openalex.org/S139212996","display_name":"Clinical Chemistry","issn_l":"0009-9147","issn":["0009-9147","1530-8561"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320272","host_organization_name":"American Association for Clinical Chemistry","host_organization_lineage":["https://openalex.org/P4310320272"],"host_organization_lineage_names":["American Association for Clinical Chemistry"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Clinical Chemistry","raw_type":"journal-article"},{"id":"pmid:10430777","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10430777","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Clinical chemistry","raw_type":"Journal Article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.324.8918","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.324.8918","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://www.clinchem.org/content/45/8/1141.full.pdf","raw_type":"text"},{"id":"pmh:oai:freidok.uni-freiburg.de:28114","doi":null,"is_oa":false,"landing_page_url":"https://freidok.uni-freiburg.de/data/28114","pdf_url":null,"source":{"id":"https://openalex.org/S4306401057","display_name":"FreiDok plus (Universit\u00e4tsbibliothek Freiburg)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I161046081","host_organization_name":"University of Freiburg","host_organization_lineage":["https://openalex.org/I161046081"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Clin Chem. - 45 (1999) , 1141 - 1147","raw_type":"article"},{"id":"mag:1955929846","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10430777","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.8371999859809875}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":44,"referenced_works":["https://openalex.org/W11993479","https://openalex.org/W15186908","https://openalex.org/W20164601","https://openalex.org/W100549942","https://openalex.org/W143256514","https://openalex.org/W1505365469","https://openalex.org/W1569947224","https://openalex.org/W1576874044","https://openalex.org/W1651383390","https://openalex.org/W1965430426","https://openalex.org/W1986314128","https://openalex.org/W1988220558","https://openalex.org/W1991267989","https://openalex.org/W2005521257","https://openalex.org/W2010855729","https://openalex.org/W2013519304","https://openalex.org/W2019288084","https://openalex.org/W2034888656","https://openalex.org/W2039558934","https://openalex.org/W2039831544","https://openalex.org/W2043951336","https://openalex.org/W2049280274","https://openalex.org/W2059589608","https://openalex.org/W2070627144","https://openalex.org/W2071692287","https://openalex.org/W2074435495","https://openalex.org/W2091066652","https://openalex.org/W2093246832","https://openalex.org/W2096814647","https://openalex.org/W2098610461","https://openalex.org/W2115112300","https://openalex.org/W2120352427","https://openalex.org/W2124671503","https://openalex.org/W2135565172","https://openalex.org/W2144345532","https://openalex.org/W2169303393","https://openalex.org/W2194992135","https://openalex.org/W2281293425","https://openalex.org/W2327467387","https://openalex.org/W2336672206","https://openalex.org/W2417963531","https://openalex.org/W2481815373","https://openalex.org/W2883545750","https://openalex.org/W3022405712"],"related_works":["https://openalex.org/W1955929846","https://openalex.org/W1983511867","https://openalex.org/W2010855729","https://openalex.org/W7219811299","https://openalex.org/W7128336515","https://openalex.org/W2736462264","https://openalex.org/W1983776986","https://openalex.org/W2094431231","https://openalex.org/W2386656841","https://openalex.org/W143256514"],"abstract_inverted_index":{"BACKGROUND:":[0],"Many":[1],"studies":[2],"have":[3,11],"convincingly":[4],"shown":[5,43],"that":[6,83,239],"survivors":[7],"of":[8,16,19,34,64,79,91,97,148,155,168,196,203,227,249],"myocardial":[9],"infarction":[10],"impaired":[12],"fibrinolytic":[13],"activity":[14],"because":[15],"increased":[17,164],"concentrations":[18],"plasma":[20,48],"plasminogen":[21],"activator":[22],"inhibitor-1":[23],"(PAI-1).":[24],"A":[25],"single":[26],"guanosine":[27],"insertion/deletion":[28],"polymorphism":[29,82,230],"in":[30,114,119,141],"the":[31,35,65,80,92,104,115,120,128,133,138,149,153,156,162,166,169,194,209,228,232],"promoter":[32],"region":[33],"PAI1":[36],"gene,":[37],"commonly":[38],"called":[39],"4G/5G,":[40],"has":[41],"been":[42],"to":[44,54,213],"be":[45],"associated":[46],"with":[47,88,208,216,231],"PAI-1":[49],"activity.":[50],"Our":[51],"aim":[52],"was":[53,159,180],"develop":[55],"and":[56,95,172,219,246],"validate":[57],"a":[58,74,173,235],"homogeneous":[59],"assay":[60],"for":[61,77,177,242],"rapid":[62],"genotyping":[63,78,226],"4G/5G":[66,81,229],"polymorphism.":[67],"METHODS:":[68],"In":[69],"this":[70,184],"report":[71],"we":[72],"present":[73,118],"single-tube":[75],"method":[76,238],"combines":[84],"both":[85,244],"rapid-cycle":[86],"PCR":[87,218],"real-time":[89,146],"monitoring":[90,147],"amplification":[93,150],"process":[94],"generation":[96],"allele-specific":[98],"fluorescent":[99],"probe":[100,126],"melting":[101,175],"profiles":[102],"on":[103],"LightCycler(TM).":[105],"Two":[106],"fluorescently":[107],"labeled":[108],"hybridization":[109],"probes":[110],"recognizing":[111],"adjacent":[112],"sequences":[113],"amplicon":[116],"were":[117,140,188,211],"reaction":[121],"mixture.":[122],"The":[123,201,225],"shorter":[124],"detection":[125],"spanned":[127],"polymorphic":[129],"site,":[130],"perfectly":[131],"matching":[132],"5G":[134],"allele.":[135],"After":[136],"annealing,":[137],"fluorophores":[139],"resonance":[142],"energy":[143],"transfer,":[144],"providing":[145],"process.":[151],"At":[152],"completion":[154],"PCR,":[157],"fluorescence":[158],"monitored":[160],"as":[161],"temperature":[163],"through":[165],"T(m)":[167],"probe/product":[170],"duplex,":[171],"characteristic":[174],"profile":[176],"each":[178],"genotype":[179],"obtained.":[181],"RESULTS:":[182],"With":[183],"method,":[185],"32":[186],"samples":[187,206],"genotyped":[189],"within":[190],"30":[191],"min":[192],"without":[193],"need":[195],"any":[197],"post-PCR":[198],"sample":[199],"manipulation.":[200],"genotypes":[202],"100":[204],"DNA":[205],"determined":[207],"LightCycler":[210,233],"identical":[212],"those":[214],"obtained":[215],"conventional":[217],"restriction":[220],"fragment":[221],"length":[222],"analysis.":[223],"CONCLUSION:":[224],"is":[234,240],"rapid,":[236],"reliable":[237],"suitable":[241],"typing":[243],"small":[245],"large":[247],"numbers":[248],"samples.":[250]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2023-02-27T00:00:00"}