{"id":"https://openalex.org/W4321374444","doi":"https://doi.org/10.3390/fishes8020118","title":"CRISPR/Cas9-Mediated Disruption of Endo16 Cis-Regulatory Elements in Sea Urchin Embryos","display_name":"CRISPR/Cas9-Mediated Disruption of Endo16 Cis-Regulatory Elements in Sea Urchin Embryos","publication_year":2023,"publication_date":"2023-02-20","ids":{"openalex":"https://openalex.org/W4321374444","doi":"https://doi.org/10.3390/fishes8020118"},"language":"en","primary_location":{"id":"doi:10.3390/fishes8020118","doi":"https://doi.org/10.3390/fishes8020118","is_oa":true,"landing_page_url":"https://doi.org/10.3390/fishes8020118","pdf_url":"https://www.mdpi.com/2410-3888/8/2/118/pdf?version=1676877842","source":{"id":"https://openalex.org/S4210229736","display_name":"Fishes","issn_l":"2410-3888","issn":["2410-3888"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","norway-1","poland-20","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Fishes","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.mdpi.com/2410-3888/8/2/118/pdf?version=1676877842","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Lili Xing","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I170897317","display_name":"Duke University","ror":"https://ror.org/00py81415","country_code":"US","type":"education","lineage":["https://openalex.org/I170897317"]},{"id":"https://openalex.org/I19820366","display_name":"Chinese Academy of Sciences","ror":"https://ror.org/034t30j35","country_code":"CN","type":"government","lineage":["https://openalex.org/I19820366"]},{"id":"https://openalex.org/I4210101167","display_name":"Institute of Oceanology","ror":"https://ror.org/018yw5541","country_code":"CN","type":"facility","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210101167"]},{"id":"https://openalex.org/I4210113896","display_name":"Qingdao National Laboratory for Marine Science and Technology","ror":"https://ror.org/026sv7t11","country_code":"CN","type":"facility","lineage":["https://openalex.org/I4210113896"]},{"id":"https://openalex.org/I4210165038","display_name":"University of Chinese Academy of Sciences","ror":"https://ror.org/05qbk4x57","country_code":"CN","type":"education","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210165038"]}],"countries":["CN","US"],"is_corresponding":false,"raw_author_name":"Lili Xing","raw_affiliation_strings":["CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China","Department of Biology, Duke University, Durham, NC 27708, USA","Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China","University of Chinese Academy of Sciences, Beijing 100049, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China","institution_ids":["https://openalex.org/I19820366","https://openalex.org/I4210101167"]},{"raw_affiliation_string":"Department of Biology, Duke University, Durham, NC 27708, USA","institution_ids":["https://openalex.org/I170897317"]},{"raw_affiliation_string":"Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China","institution_ids":["https://openalex.org/I4210113896"]},{"raw_affiliation_string":"University of Chinese Academy of Sciences, Beijing 100049, China","institution_ids":["https://openalex.org/I4210165038"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5083595341","display_name":"Lingyu Wang","orcid":"https://orcid.org/0000-0002-2736-7588","observed_orcids":["https://orcid.org/0000-0002-2736-7588"]},"institutions":[{"id":"https://openalex.org/I170897317","display_name":"Duke University","ror":"https://ror.org/00py81415","country_code":"US","type":"education","lineage":["https://openalex.org/I170897317"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Lingyu Wang","raw_affiliation_strings":["Department of Biology, Duke University, Durham, NC 27708, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, Duke University, Durham, NC 27708, USA","institution_ids":["https://openalex.org/I170897317"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5136369043","display_name":"Femke Roos","orcid":"https://orcid.org/0009-0001-9109-7455","observed_orcids":["https://orcid.org/0009-0001-9109-7455"]},"institutions":[{"id":"https://openalex.org/I103855431","display_name":"HAN University of Applied Sciences","ror":"https://ror.org/0500gea42","country_code":"NL","type":"education","lineage":["https://openalex.org/I103855431"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Femke Roos","raw_affiliation_strings":["School of Applied Biosciences and Chemistry, HAN University of Applied-Sciences, 6500 Jk Nijmegen, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Applied Biosciences and Chemistry, HAN University of Applied-Sciences, 6500 Jk Nijmegen, The Netherlands","institution_ids":["https://openalex.org/I103855431"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100728425","display_name":"Michelle Lee","orcid":"https://orcid.org/0000-0003-3854-1294","observed_orcids":["https://orcid.org/0000-0003-3854-1294"]},"institutions":[{"id":"https://openalex.org/I170897317","display_name":"Duke University","ror":"https://ror.org/00py81415","country_code":"US","type":"education","lineage":["https://openalex.org/I170897317"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michelle Lee","raw_affiliation_strings":["Department of Biology, Duke University, Durham, NC 27708, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, Duke University, Durham, NC 27708, USA","institution_ids":["https://openalex.org/I170897317"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5081032810","display_name":"Gregory A. Wray","orcid":"https://orcid.org/0000-0001-5634-5081","observed_orcids":["https://orcid.org/0000-0001-5634-5081"]},"institutions":[{"id":"https://openalex.org/I170897317","display_name":"Duke University","ror":"https://ror.org/00py81415","country_code":"US","type":"education","lineage":["https://openalex.org/I170897317"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Gregory A. Wray","raw_affiliation_strings":["Department of Biology, Duke University, Durham, NC 27708, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, Duke University, Durham, NC 27708, USA","institution_ids":["https://openalex.org/I170897317"]}]}],"institutions":[],"countries_distinct_count":3,"institutions_distinct_count":6,"corresponding_author_ids":["https://openalex.org/A5081032810"],"corresponding_institution_ids":["https://openalex.org/I170897317"],"apc_list":{"value":1800,"currency":"CHF","value_usd":2002},"apc_paid":{"value":1800,"currency":"CHF","value_usd":2002},"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.02315027,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"8","issue":"2","first_page":"118","last_page":"118"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10268","display_name":"Developmental Biology and Gene Regulation","score":0.45579999685287476,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10268","display_name":"Developmental Biology and Gene Regulation","score":0.45579999685287476,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.4154999852180481,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12841","display_name":"Echinoderm biology and ecology","score":0.05119999870657921,"subfield":{"id":"https://openalex.org/subfields/1104","display_name":"Aquatic Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cis-regulatory-elements","display_name":"cis-regulatory elements","score":0.9919999837875366},{"id":"https://openalex.org/keywords/enhancers","display_name":"enhancers","score":0.8029999732971191},{"id":"https://openalex.org/keywords/lytechinus-variegatus","display_name":"Lytechinus variegatus","score":0.8769999742507935},{"id":"https://openalex.org/keywords/sea-urchin-embryo","display_name":"sea urchin embryo","score":0.9369999766349792},{"id":"https://openalex.org/keywords/crispr-cas9-gene-disruption","display_name":"CRISPR/Cas9 gene disruption","score":0.8579999804496765},{"id":"https://openalex.org/keywords/gastrulation","display_name":"gastrulation","score":0.7649999856948853},{"id":"https://openalex.org/keywords/phenocopy","display_name":"phenocopy","score":0.5519999861717224},{"id":"https://openalex.org/keywords/morpholino-knockdown","display_name":"morpholino knockdown","score":0.8399999737739563},{"id":"https://openalex.org/keywords/aquaculture","display_name":"aquaculture","score":0.847000002861023}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8346350193023682},{"id":"https://openalex.org/C111936080","wikidata":"https://www.wikidata.org/wiki/Q913367","display_name":"Enhancer","level":4,"score":0.6793020963668823},{"id":"https://openalex.org/C98108389","wikidata":"https://www.wikidata.org/wiki/Q412563","display_name":"CRISPR","level":3,"score":0.677918553352356},{"id":"https://openalex.org/C129536746","wikidata":"https://www.wikidata.org/wiki/Q1141026","display_name":"Gastrulation","level":4,"score":0.6327784657478333},{"id":"https://openalex.org/C14429324","wikidata":"https://www.wikidata.org/wiki/Q1191982","display_name":"Mariculture","level":4,"score":0.540002703666687},{"id":"https://openalex.org/C2778403493","wikidata":"https://www.wikidata.org/wiki/Q2302585","display_name":"Lytechinus variegatus","level":3,"score":0.5164321064949036},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.4877684712409973},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.48083147406578064},{"id":"https://openalex.org/C132455925","wikidata":"https://www.wikidata.org/wiki/Q16965677","display_name":"Cas9","level":4,"score":0.4671066999435425},{"id":"https://openalex.org/C136571751","wikidata":"https://www.wikidata.org/wiki/Q424607","display_name":"Morpholino","level":4,"score":0.4594266414642334},{"id":"https://openalex.org/C2776878037","wikidata":"https://www.wikidata.org/wiki/Q169444","display_name":"Zebrafish","level":3,"score":0.4415940046310425},{"id":"https://openalex.org/C2776908312","wikidata":"https://www.wikidata.org/wiki/Q83483","display_name":"Sea urchin","level":2,"score":0.39534321427345276},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.39530012011528015},{"id":"https://openalex.org/C86339819","wikidata":"https://www.wikidata.org/wiki/Q407384","display_name":"Transcription factor","level":3,"score":0.339092880487442},{"id":"https://openalex.org/C78458016","wikidata":"https://www.wikidata.org/wiki/Q840400","display_name":"Evolutionary biology","level":1,"score":0.33365416526794434},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.33211469650268555},{"id":"https://openalex.org/C505870484","wikidata":"https://www.wikidata.org/wiki/Q180538","display_name":"Fishery","level":1,"score":0.24306052923202515},{"id":"https://openalex.org/C86909935","wikidata":"https://www.wikidata.org/wiki/Q188989","display_name":"Aquaculture","level":3,"score":0.22818586230278015},{"id":"https://openalex.org/C87073359","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryogenesis","level":3,"score":0.18571460247039795},{"id":"https://openalex.org/C2909208804","wikidata":"https://www.wikidata.org/wiki/Q127282","display_name":"Fish <Actinopterygii>","level":2,"score":0.11282256245613098}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.3390/fishes8020118","doi":"https://doi.org/10.3390/fishes8020118","is_oa":true,"landing_page_url":"https://doi.org/10.3390/fishes8020118","pdf_url":"https://www.mdpi.com/2410-3888/8/2/118/pdf?version=1676877842","source":{"id":"https://openalex.org/S4210229736","display_name":"Fishes","issn_l":"2410-3888","issn":["2410-3888"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","norway-1","poland-20","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Fishes","raw_type":"journal-article"},{"id":"pmh:oai:doaj.org/article:337e097aac694e8fa937865af4e0246b","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/337e097aac694e8fa937865af4e0246b","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Fishes, Vol 8, Iss 2, p 118 (2023)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.3390/fishes8020118","doi":"https://doi.org/10.3390/fishes8020118","is_oa":true,"landing_page_url":"https://doi.org/10.3390/fishes8020118","pdf_url":"https://www.mdpi.com/2410-3888/8/2/118/pdf?version=1676877842","source":{"id":"https://openalex.org/S4210229736","display_name":"Fishes","issn_l":"2410-3888","issn":["2410-3888"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","norway-1","poland-20","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Fishes","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2653063555","display_name":"Evolution of a developmental gene regulatory network during a life history switch in Heliocidaris","funder_award_id":"1929934","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"},{"id":"https://openalex.org/G4432437846","display_name":null,"funder_award_id":"42106109","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"}],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"},{"id":"https://openalex.org/F4320321001","display_name":"National Natural Science Foundation of China","ror":"https://ror.org/01h0zpd94"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4321374444.pdf","grobid_xml":"https://content.openalex.org/works/W4321374444.grobid-xml"},"referenced_works_count":46,"referenced_works":["https://openalex.org/W1596040889","https://openalex.org/W1968952506","https://openalex.org/W1976391205","https://openalex.org/W1983640121","https://openalex.org/W2001006409","https://openalex.org/W2002404684","https://openalex.org/W2008694033","https://openalex.org/W2008989169","https://openalex.org/W2012354488","https://openalex.org/W2078059415","https://openalex.org/W2079358769","https://openalex.org/W2089806726","https://openalex.org/W2096261947","https://openalex.org/W2114570899","https://openalex.org/W2122960292","https://openalex.org/W2144831379","https://openalex.org/W2152435464","https://openalex.org/W2152956404","https://openalex.org/W2152973976","https://openalex.org/W2153800196","https://openalex.org/W2157164705","https://openalex.org/W2168012232","https://openalex.org/W2179936663","https://openalex.org/W2217477210","https://openalex.org/W2272903816","https://openalex.org/W2439063595","https://openalex.org/W2475217470","https://openalex.org/W2489736413","https://openalex.org/W2550630956","https://openalex.org/W2551467732","https://openalex.org/W2626683726","https://openalex.org/W2752929029","https://openalex.org/W2803843936","https://openalex.org/W2888908334","https://openalex.org/W2945071325","https://openalex.org/W2945620350","https://openalex.org/W3005471404","https://openalex.org/W3009712380","https://openalex.org/W3044199366","https://openalex.org/W3124768367","https://openalex.org/W3168186318","https://openalex.org/W3179232999","https://openalex.org/W3190238055","https://openalex.org/W3199565759","https://openalex.org/W3200211450","https://openalex.org/W3208990611"],"related_works":["https://openalex.org/W2179936663","https://openalex.org/W2965677013","https://openalex.org/W7100151921","https://openalex.org/W2626683726","https://openalex.org/W88083536","https://openalex.org/W2902364702","https://openalex.org/W2144831379","https://openalex.org/W2103985802","https://openalex.org/W4318617358","https://openalex.org/W4390077244"],"abstract_inverted_index":{"Sea":[0],"urchins":[1],"have":[2],"become":[3],"significant":[4],"mariculture":[5],"species":[6],"globally,":[7],"and":[8,23,100,103,131,161,174,182],"also":[9],"serve":[10],"as":[11],"invertebrate":[12],"model":[13],"organisms":[14],"in":[15,63,68,96,171],"developmental":[16],"biology.":[17],"Cis-regulatory":[18,42],"elements":[19,170],"(enhancers)":[20],"control":[21],"development":[22],"physiology":[24],"by":[25],"regulating":[26],"gene":[27],"expression.":[28],"Mutations":[29],"that":[30,105],"affect":[31],"the":[32,49,54,71,97,166],"function":[33,167],"of":[34,61,70,90,125,168],"these":[35,137],"sequences":[36],"may":[37],"contribute":[38],"to":[39,57,111,135,176],"phenotypic":[40],"diversity.":[41],"targets":[43],"offer":[44],"new":[45],"breeding":[46,179],"potential":[47,152],"for":[48,164],"future.":[50],"Here,":[51],"we":[52,128,149],"use":[53],"CRISPR/Cas9":[55],"system":[56],"disrupt":[58],"an":[59],"enhancer":[60],"Endo16":[62,83,106],"developing":[64],"Lytechinus":[65],"variegatus":[66],"embryos,":[67],"consideration":[69],"thorough":[72],"research":[73],"on":[74],"Endo16\u2019s":[75],"regulatory":[76,91],"region.":[77],"We":[78],"designed":[79],"six":[80],"gRNAs":[81],"against":[82],"Module":[84,107],"A":[85],"(the":[86],"most":[87],"proximal":[88],"region":[89],"sequences,":[92],"which":[93],"activates":[94],"transcription":[95],"vegetal":[98],"plate":[99],"archenteron,":[101],"specifically)":[102],"discovered":[104],"A-disrupted":[108],"embryos":[109],"failed":[110],"undergo":[112],"gastrulation":[113],"at":[114],"20":[115],"h":[116],"post":[117],"fertilization.":[118],"This":[119],"result":[120],"partly":[121],"phenocopies":[122],"morpholino":[123],"knockdowns":[124],"Endo16.":[126],"Moreover,":[127],"conducted":[129],"qPCR":[130],"clone":[132],"sequencing":[133,146],"experiments":[134],"verify":[136],"results.":[138],"Although":[139],"mutations":[140],"were":[141],"not":[142],"found":[143],"regularly":[144],"from":[145],"affected":[147],"individuals,":[148],"discuss":[150],"some":[151],"causes.":[153],"In":[154],"conclusion,":[155],"our":[156],"study":[157],"provides":[158],"a":[159],"feasible":[160],"informative":[162],"method":[163],"studying":[165],"cis-regulatory":[169],"sea":[172],"urchins,":[173],"contributes":[175],"echinoderm":[177],"precision":[178],"technology":[180],"innovation":[181],"aquaculture":[183],"industry":[184],"development.":[185]},"counts_by_year":[],"updated_date":"2026-10-16T09:59:22.235173","created_date":"2023-02-21T00:00:00"}