{"id":"https://openalex.org/W4320032589","doi":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2023.01.032","title":"[Using Next-Generation Sequencing Technology to Confirm the HLA Rare Alleles Detected by PCR-SSOP].","display_name":"[Using Next-Generation Sequencing Technology to Confirm the HLA Rare Alleles Detected by PCR-SSOP].","publication_year":2023,"publication_date":"2023-02-01","ids":{"openalex":"https://openalex.org/W4320032589","doi":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2023.01.032","pmid":"https://pubmed.ncbi.nlm.nih.gov/36765500"},"language":"zh","primary_location":{"id":"pmid:36765500","doi":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2023.01.032","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/36765500","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Zhongguo shi yan xue ye xue za zhi","raw_type":"English Abstract"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5087635098","display_name":"Xianxin Zhong","orcid":"https://orcid.org/0009-0007-3009-0667","observed_orcids":["https://orcid.org/0009-0007-3009-0667"]},"institutions":[{"id":"https://openalex.org/I4405273710","display_name":"Shenzhen Traditional Chinese Medicine Hospital","ror":"https://ror.org/00b16vp10","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4405273710"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xian-Xin Zhong","raw_affiliation_strings":["Department of Clinical Laboratory Examination, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen 518033, Guangdong Province, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Clinical Laboratory Examination, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen 518033, Guangdong Province, China","institution_ids":["https://openalex.org/I4405273710"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5060477373","display_name":"Wang\u2010da Wu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405273710","display_name":"Shenzhen Traditional Chinese Medicine Hospital","ror":"https://ror.org/00b16vp10","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4405273710"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Wang-Da Wu","raw_affiliation_strings":["Department of Clinical Laboratory Examination, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen 518033, Guangdong Province, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Clinical Laboratory Examination, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen 518033, Guangdong Province, China","institution_ids":["https://openalex.org/I4405273710"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5076182951","display_name":"Zhan\u2010Rou Quan","orcid":"https://orcid.org/0000-0002-1733-2015","observed_orcids":["https://orcid.org/0000-0002-1733-2015"]},"institutions":[{"id":"https://openalex.org/I4210159978","display_name":"Shenzhen Blood Center","ror":"https://ror.org/04tans503","country_code":"CN","type":"nonprofit","lineage":["https://openalex.org/I4210159978"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Zhan-Rou Quan","raw_affiliation_strings":["Institute of Transfusion Medicine of Shenzhen Blood Center, Shenzhen 518020, Guangdong Province, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Transfusion Medicine of Shenzhen Blood Center, Shenzhen 518020, Guangdong Province, China","institution_ids":["https://openalex.org/I4210159978"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5034913472","display_name":"Su-qing Gao","orcid":"https://orcid.org/0000-0002-9407-8065","observed_orcids":["https://orcid.org/0000-0002-9407-8065"]},"institutions":[{"id":"https://openalex.org/I4210159978","display_name":"Shenzhen Blood Center","ror":"https://ror.org/04tans503","country_code":"CN","type":"nonprofit","lineage":["https://openalex.org/I4210159978"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Su-Qing Gao","raw_affiliation_strings":["Institute of Transfusion Medicine of Shenzhen Blood Center, Shenzhen 518020, Guangdong Province, China.E-mail: gaosuqing1@163.com"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Transfusion Medicine of Shenzhen Blood Center, Shenzhen 518020, Guangdong Province, China.E-mail: gaosuqing1@163.com","institution_ids":["https://openalex.org/I4210159978"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"31","issue":"1","first_page":"203","last_page":"208"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10345","display_name":"Hematopoietic Stem Cell Transplantation","score":0.9276999831199646,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10345","display_name":"Hematopoietic Stem Cell Transplantation","score":0.9276999831199646,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10373","display_name":"Renal Transplantation Outcomes and Treatments","score":0.035999998450279236,"subfield":{"id":"https://openalex.org/subfields/2747","display_name":"Transplantation"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10261","display_name":"Genetic Associations and Epidemiology","score":0.012900000438094139,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hla-typing","display_name":"HLA typing","score":0.8370000123977661},{"id":"https://openalex.org/keywords/rare-hla-alleles","display_name":"rare HLA alleles","score":0.9300000071525574},{"id":"https://openalex.org/keywords/novel-hla-allele","display_name":"novel HLA allele","score":0.871999979019165},{"id":"https://openalex.org/keywords/next-generation-sequencing","display_name":"next-generation sequencing","score":0.8090000152587891},{"id":"https://openalex.org/keywords/hematopoietic-stem-cell-donors","display_name":"hematopoietic stem cell donors","score":0.7689999938011169}],"concepts":[{"id":"https://openalex.org/C31467283","wikidata":"https://www.wikidata.org/wiki/Q912147","display_name":"Genotyping","level":4,"score":0.8254777193069458},{"id":"https://openalex.org/C135763542","wikidata":"https://www.wikidata.org/wiki/Q106016","display_name":"Genotype","level":3,"score":0.7210934162139893},{"id":"https://openalex.org/C2781209916","wikidata":"https://www.wikidata.org/wiki/Q3215756","display_name":"Typing","level":2,"score":0.5880523920059204},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5690822005271912},{"id":"https://openalex.org/C180754005","wikidata":"https://www.wikidata.org/wiki/Q80726","display_name":"Allele","level":3,"score":0.5449430346488953},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.4691532850265503},{"id":"https://openalex.org/C188280979","wikidata":"https://www.wikidata.org/wiki/Q911125","display_name":"Human leukocyte antigen","level":3,"score":0.4661368727684021},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.45561230182647705},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.4094894528388977},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.14880526065826416},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.10329842567443848}],"mesh":[{"descriptor_ui":"D000483","descriptor_name":"Alleles","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005838","descriptor_name":"Genotype","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006650","descriptor_name":"Histocompatibility Testing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013672","descriptor_name":"Technology","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D059014","descriptor_name":"High-Throughput Nucleotide Sequencing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":1,"locations":[{"id":"pmid:36765500","doi":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2023.01.032","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/36765500","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Zhongguo shi yan xue ye xue za zhi","raw_type":"English Abstract"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.8560000061988831,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2955795503","https://openalex.org/W2889363253","https://openalex.org/W3032116844","https://openalex.org/W2368018040","https://openalex.org/W3033093923","https://openalex.org/W4299864607","https://openalex.org/W2230917698","https://openalex.org/W3013250047","https://openalex.org/W2775287552","https://openalex.org/W2530580903"],"abstract_inverted_index":{"OBJECTIVE:":[0],"To":[1],"confirm":[2],"the":[3,7,87,95],"HLA":[4,16,40,57,107],"genotypes":[5],"of":[6,12,24,50,53,65,94,106,110],"samples":[8,37,52,64],"including":[9],"4":[10,51],"cases":[11,23],"magnetic":[13,54,111],"bead":[14,55],"probe":[15,32,56,112],"genotyping":[17,58,108],"result":[18,26,59,67],"pattern":[19,60,105],"abnormality":[20,61],"and":[21,62,77,89],"3":[22,63],"ambiguous":[25,66],"detected":[27,45],"by":[28,46,71,113,133],"PCR":[29,72],"sequence-specific":[30],"oligonudeotide":[31],"(SSOP)":[33],"method.":[34],"METHODS:":[35],"All":[36],"derived":[38],"from":[39],"high-resolution":[41],"typing":[42,74],"laboratory":[43],"were":[44,68,83],"PCR-SSOP.":[47],"A":[48],"total":[49],"further":[69],"confirmed":[70],"sequence-based":[73],"(SBT)":[75],"technology":[76],"next-generation":[78],"sequencing":[79],"(NGS)":[80],"technology.":[81],"RESULTS:":[82],"not":[84],"included":[85],"in":[86],"Common":[88],"Well-documented":[90],"Alleles":[91],"(CWD)":[92],"v2.4":[93],"Chinese":[96],"Hematopoietic":[97],"Stem":[98],"Cell":[99],"Donor":[100],"Database.":[101],"CONCLUSION:":[102],"The":[103],"abnormal":[104],"results":[109],"PCR-SSOP":[114],"method":[115],"suggests":[116],"that":[117],"it":[118],"may":[119],"be":[120,131],"a":[121,125],"rare":[122],"allele":[123],"or":[124],"novel":[126],"allele,":[127],"which":[128],"needs":[129],"to":[130],"verified":[132],"sequencing.":[134]},"counts_by_year":[],"updated_date":"2026-11-02T09:45:03.842371","created_date":"2023-02-12T00:00:00"}