{"id":"https://openalex.org/W4313316346","doi":"https://doi.org/10.4049/jimmunol.103.4.809","title":"The Binding Reactions of Antibodies Specific for the 2,6-Dintrophenyl Group","display_name":"The Binding Reactions of Antibodies Specific for the 2,6-Dintrophenyl Group","publication_year":1969,"publication_date":"1969-10-01","ids":{"openalex":"https://openalex.org/W4313316346","doi":"https://doi.org/10.4049/jimmunol.103.4.809","mag":"1774315852","pmid":"https://pubmed.ncbi.nlm.nih.gov/4981687"},"language":"en","primary_location":{"id":"doi:10.4049/jimmunol.103.4.809","doi":"https://doi.org/10.4049/jimmunol.103.4.809","is_oa":false,"landing_page_url":"https://doi.org/10.4049/jimmunol.103.4.809","pdf_url":null,"source":{"id":"https://openalex.org/S38008053","display_name":"The Journal of Immunology","issn_l":"0022-1767","issn":["0022-1767","1550-6606"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4320800377","host_organization_name":"American Association of Immunologists","host_organization_lineage":["https://openalex.org/P4320800377"],"host_organization_lineage_names":["American Association of Immunologists"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Immunology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"J Russell Little","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I204465549","display_name":"Washington University in St. Louis","ror":"https://ror.org/01yc7t268","country_code":"US","type":"education","lineage":["https://openalex.org/I204465549"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"J Russell Little","raw_affiliation_strings":["Departments of Microbiology and Medicine (at the Jewish Hospital), Washington University School of Medicine , St. Louis, Missouri 63110"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Microbiology and Medicine (at the Jewish Hospital), Washington University School of Medicine , St. Louis, Missouri 63110","institution_ids":["https://openalex.org/I204465549"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5037282171","display_name":"Wayne A. Border","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I204465549","display_name":"Washington University in St. Louis","ror":"https://ror.org/01yc7t268","country_code":"US","type":"education","lineage":["https://openalex.org/I204465549"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Wayne Border","raw_affiliation_strings":["Departments of Microbiology and Medicine (at the Jewish Hospital), Washington University School of Medicine , St. Louis, Missouri 63110"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Microbiology and Medicine (at the Jewish Hospital), Washington University School of Medicine , St. Louis, Missouri 63110","institution_ids":["https://openalex.org/I204465549"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5037973180","display_name":"Ralph B. Freidin","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I204465549","display_name":"Washington University in St. Louis","ror":"https://ror.org/01yc7t268","country_code":"US","type":"education","lineage":["https://openalex.org/I204465549"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ralph Freidin","raw_affiliation_strings":["Departments of Microbiology and Medicine (at the Jewish Hospital), Washington University School of Medicine , St. Louis, Missouri 63110"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Microbiology and Medicine (at the Jewish Hospital), Washington University School of Medicine , St. Louis, Missouri 63110","institution_ids":["https://openalex.org/I204465549"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I204465549"],"apc_list":{"value":3500,"currency":"USD","value_usd":3500},"apc_paid":null,"fwci":0.5262,"has_fulltext":false,"cited_by_count":11,"citation_normalized_percentile":{"value":0.69848889,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"103","issue":"4","first_page":"809","last_page":"817"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.9940999746322632,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.9940999746322632,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10031","display_name":"T-cell and B-cell Immunology","score":0.0026000000070780516,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12576","display_name":"vaccines and immunoinformatics approaches","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/antigen-binding-site","display_name":"antibody binding sites","score":0.5989999771118164},{"id":"https://openalex.org/keywords/tryptophan-residues","display_name":"tryptophan residues","score":0.9200000166893005},{"id":"https://openalex.org/keywords/2-4-dinitrophenyl","display_name":"2,4-dinitrophenyl","score":0.7799999713897705},{"id":"https://openalex.org/keywords/2-4-6-trinitrophenyl","display_name":"2,4,6-trinitrophenyl","score":0.9900000095367432},{"id":"https://openalex.org/keywords/hapten-antibody-binding","display_name":"hapten-antibody binding","score":0.656000018119812},{"id":"https://openalex.org/keywords/wavelength-shift","display_name":"wavelength shift","score":0.382999986410141},{"id":"https://openalex.org/keywords/cross-reactivity","display_name":"cross-reactivity","score":0.7020000219345093}],"concepts":[{"id":"https://openalex.org/C29144244","wikidata":"https://www.wikidata.org/wiki/Q506441","display_name":"Hapten","level":3,"score":0.8275206089019775},{"id":"https://openalex.org/C2778815084","wikidata":"https://www.wikidata.org/wiki/Q79803","display_name":"Guinea pig","level":2,"score":0.7350703477859497},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.7091375589370728},{"id":"https://openalex.org/C46051903","wikidata":"https://www.wikidata.org/wiki/Q909339","display_name":"Bathochromic shift","level":3,"score":0.6850248575210571},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6665798425674438},{"id":"https://openalex.org/C2776706248","wikidata":"https://www.wikidata.org/wiki/Q181003","display_name":"Tryptophan","level":3,"score":0.5493884682655334},{"id":"https://openalex.org/C32909587","wikidata":"https://www.wikidata.org/wiki/Q11369","display_name":"Molecule","level":2,"score":0.49717238545417786},{"id":"https://openalex.org/C2780659158","wikidata":"https://www.wikidata.org/wiki/Q2466273","display_name":"Immunochemistry","level":3,"score":0.42737385630607605},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.3761962652206421},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.37081751227378845},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.35345348715782166},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.26462385058403015},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.21746373176574707},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.1769167184829712},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.14584454894065857},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.07853582501411438},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.07145282626152039},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000614","descriptor_name":"Aminocaproates","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000906","descriptor_name":"Antibodies","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D000941","descriptor_name":"Antigens","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004140","descriptor_name":"Dinitrophenols","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D005455","descriptor_name":"Fluorescent Antibody Technique","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005779","descriptor_name":"Immunodiffusion","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006168","descriptor_name":"Guinea Pigs","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006241","descriptor_name":"Haptens","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D011232","descriptor_name":"Chemical Precipitation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011817","descriptor_name":"Rabbits","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013057","descriptor_name":"Spectrum Analysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014364","descriptor_name":"Tryptophan","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.4049/jimmunol.103.4.809","doi":"https://doi.org/10.4049/jimmunol.103.4.809","is_oa":false,"landing_page_url":"https://doi.org/10.4049/jimmunol.103.4.809","pdf_url":null,"source":{"id":"https://openalex.org/S38008053","display_name":"The Journal of Immunology","issn_l":"0022-1767","issn":["0022-1767","1550-6606"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4320800377","host_organization_name":"American Association of Immunologists","host_organization_lineage":["https://openalex.org/P4320800377"],"host_organization_lineage_names":["American Association of Immunologists"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Immunology","raw_type":"journal-article"},{"id":"pmid:4981687","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/4981687","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of immunology (Baltimore, Md. : 1950)","raw_type":"Journal Article"},{"id":"mag:1774315852","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/4981687","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1774315852","https://openalex.org/W103029564","https://openalex.org/W1606893225","https://openalex.org/W2129193800","https://openalex.org/W1571316787","https://openalex.org/W3169624002","https://openalex.org/W4313315415","https://openalex.org/W1780736830","https://openalex.org/W7100869082","https://openalex.org/W2044320476"],"abstract_inverted_index":{"Abstract":[0],"Rabbit":[1,45],"and":[2,18,33,65],"guinea":[3,78],"pig":[4,79],"antibodies":[5,40,80],"specific":[6,16,107],"for":[7,108],"the":[8,25,56,85,90,101],"2,6-DNP":[9],"group":[10],"were":[11,48,69],"isolated":[12],"from":[13],"sera":[14],"by":[15],"precipitation":[17],"hapten":[19],"elution.":[20],"Anti-2,6-DNP-antibodies":[21],"formed":[22,73],"late":[23],"in":[24,100],"immune":[26],"response":[27],"displayed":[28],"greater":[29],"cross-reactivity":[30],"with":[31,39,75,82],"2,4-DNP":[32],"2,4,6-TNP":[34],"ligands":[35],"than":[36],"was":[37],"observed":[38,70],"obtained":[41],"at":[42],"earlier":[43],"times.":[44],"anti-2,6-DNP":[46],"molecules":[47,106],"found":[49],"to":[50],"have":[51],"low":[52],"Qmax":[53],"values":[54],"(in":[55],"same":[57],"general":[58],"range":[59],"as":[60],"rabbit":[61,76],"anti-2,4,6-TNP":[62],"molecules).":[63],"Hypochromic":[64],"bathochromic":[66],"spectral":[67],"shifts":[68],"when":[71],"\u03b5-2,6-DNP-aminocaproate":[72],"complexes":[74],"or":[77,81,95],"tryptophan":[83,97],"(as":[84],"free":[86],"amino":[87],"acid)":[88],"supporting":[89],"previous":[91],"suggestion":[92],"that":[93],"one":[94],"more":[96],"residues":[98],"occur":[99],"binding":[102],"sites":[103],"of":[104],"antibody":[105],"polynitrophenyl":[109],"determinant":[110],"groups.":[111]},"counts_by_year":[{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2023-01-06T00:00:00"}