{"id":"https://openalex.org/W4313316341","doi":"https://doi.org/10.4049/jimmunol.106.2.304","title":"C1q: Rapid Purification Method for Preparation of Monospecific Antisera and for Biochemical Studies","display_name":"C1q: Rapid Purification Method for Preparation of Monospecific Antisera and for Biochemical Studies","publication_year":1971,"publication_date":"1971-02-01","ids":{"openalex":"https://openalex.org/W4313316341","doi":"https://doi.org/10.4049/jimmunol.106.2.304","mag":"1783506013","pmid":"https://pubmed.ncbi.nlm.nih.gov/5545135"},"language":"en","primary_location":{"id":"doi:10.4049/jimmunol.106.2.304","doi":"https://doi.org/10.4049/jimmunol.106.2.304","is_oa":false,"landing_page_url":"https://doi.org/10.4049/jimmunol.106.2.304","pdf_url":null,"source":{"id":"https://openalex.org/S38008053","display_name":"The Journal of Immunology","issn_l":"0022-1767","issn":["0022-1767","1550-6606"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4320800377","host_organization_name":"American Association of Immunologists","host_organization_lineage":["https://openalex.org/P4320800377"],"host_organization_lineage_names":["American Association of Immunologists"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Immunology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5016197065","display_name":"Kunio Yonemasu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I17301866","display_name":"University of Alabama","ror":"https://ror.org/03xrrjk67","country_code":"US","type":"education","lineage":["https://openalex.org/I17301866"]},{"id":"https://openalex.org/I32389192","display_name":"University of Alabama at Birmingham","ror":"https://ror.org/008s83205","country_code":"US","type":"education","lineage":["https://openalex.org/I32389192"]},{"id":"https://openalex.org/I4390039250","display_name":"UAB Medicine","ror":"https://ror.org/036554539","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4390039250"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kunio Yonemasu","raw_affiliation_strings":["Division of Clinical Immunology and Rheumatology, Department of Medicine, The Medical Center, University of Alabama in Birmingham , 1919 Seventh Avenue, South Birmingham, Alabama 35233","Division of Clinical Immunology and Rheumatology, Department of Medicine, The Medical Center, University of Alabama in Birmingham, 1919 Seventh Avenue From the , South Birmingham, Alabama 35233"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Clinical Immunology and Rheumatology, Department of Medicine, The Medical Center, University of Alabama in Birmingham , 1919 Seventh Avenue, South Birmingham, Alabama 35233","institution_ids":["https://openalex.org/I17301866","https://openalex.org/I32389192","https://openalex.org/I4390039250"]},{"raw_affiliation_string":"Division of Clinical Immunology and Rheumatology, Department of Medicine, The Medical Center, University of Alabama in Birmingham, 1919 Seventh Avenue From the , South Birmingham, Alabama 35233","institution_ids":["https://openalex.org/I17301866","https://openalex.org/I32389192","https://openalex.org/I4390039250"]}]},{"author_position":"last","author":{"id":null,"display_name":"Robert M Stroud","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I17301866","display_name":"University of Alabama","ror":"https://ror.org/03xrrjk67","country_code":"US","type":"education","lineage":["https://openalex.org/I17301866"]},{"id":"https://openalex.org/I32389192","display_name":"University of Alabama at Birmingham","ror":"https://ror.org/008s83205","country_code":"US","type":"education","lineage":["https://openalex.org/I32389192"]},{"id":"https://openalex.org/I4390039250","display_name":"UAB Medicine","ror":"https://ror.org/036554539","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4390039250"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Robert M Stroud","raw_affiliation_strings":["Division of Clinical Immunology and Rheumatology, Department of Medicine, The Medical Center, University of Alabama in Birmingham , 1919 Seventh Avenue, South Birmingham, Alabama 35233","Division of Clinical Immunology and Rheumatology, Department of Medicine, The Medical Center, University of Alabama in Birmingham, 1919 Seventh Avenue From the , South Birmingham, Alabama 35233"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Clinical Immunology and Rheumatology, Department of Medicine, The Medical Center, University of Alabama in Birmingham , 1919 Seventh Avenue, South Birmingham, Alabama 35233","institution_ids":["https://openalex.org/I17301866","https://openalex.org/I32389192","https://openalex.org/I4390039250"]},{"raw_affiliation_string":"Division of Clinical Immunology and Rheumatology, Department of Medicine, The Medical Center, University of Alabama in Birmingham, 1919 Seventh Avenue From the , South Birmingham, Alabama 35233","institution_ids":["https://openalex.org/I17301866","https://openalex.org/I32389192","https://openalex.org/I4390039250"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3500,"currency":"USD","value_usd":3500},"apc_paid":null,"fwci":5.6186,"has_fulltext":false,"cited_by_count":383,"citation_normalized_percentile":{"value":0.96288244,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"106","issue":"2","first_page":"304","last_page":"313"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10993","display_name":"Complement system in diseases","score":0.9955999851226807,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10993","display_name":"Complement system in diseases","score":0.9955999851226807,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.0019000000320374966,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T14245","display_name":"Biomarkers in Disease Mechanisms","score":0.001500000013038516,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/c1q","display_name":"C1q","score":0.9409999847412109},{"id":"https://openalex.org/keywords/complement-system","display_name":"complement system","score":0.6010000109672546},{"id":"https://openalex.org/keywords/protein-purification","display_name":"protein purification","score":0.7870000004768372},{"id":"https://openalex.org/keywords/low-ionic-strength","display_name":"low ionic strength","score":0.8980000019073486},{"id":"https://openalex.org/keywords/monospecific-antisera","display_name":"monospecific antisera","score":0.9120000004768372},{"id":"https://openalex.org/keywords/hemolytic-activity","display_name":"hemolytic activity","score":0.8799999952316284},{"id":"https://openalex.org/keywords/latex-agglutination","display_name":"latex agglutination test","score":0.9829999804496765},{"id":"https://openalex.org/keywords/molecular-weight","display_name":"molecular weight","score":0.6539999842643738},{"id":"https://openalex.org/keywords/rabbit-antiserum","display_name":"rabbit antiserum","score":0.5490000247955322}],"concepts":[{"id":"https://openalex.org/C91523082","wikidata":"https://www.wikidata.org/wiki/Q420496","display_name":"Antiserum","level":3,"score":0.9032593965530396},{"id":"https://openalex.org/C2779402116","wikidata":"https://www.wikidata.org/wiki/Q342939","display_name":"Acrylamide","level":4,"score":0.7588224411010742},{"id":"https://openalex.org/C2779390175","wikidata":"https://www.wikidata.org/wiki/Q855786","display_name":"Immunoelectrophoresis","level":3,"score":0.72673100233078},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7180181741714478},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.6529572606086731},{"id":"https://openalex.org/C143508840","wikidata":"https://www.wikidata.org/wiki/Q393294","display_name":"Agglutination (biology)","level":3,"score":0.6086388230323792},{"id":"https://openalex.org/C2778913249","wikidata":"https://www.wikidata.org/wiki/Q422241","display_name":"Sodium dodecyl sulfate","level":2,"score":0.4755686819553375},{"id":"https://openalex.org/C2780365088","wikidata":"https://www.wikidata.org/wiki/Q48318","display_name":"Urea","level":2,"score":0.45724961161613464},{"id":"https://openalex.org/C187428577","wikidata":"https://www.wikidata.org/wiki/Q898396","display_name":"Ionic strength","level":3,"score":0.4519382417201996},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.41978126764297485},{"id":"https://openalex.org/C40250595","wikidata":"https://www.wikidata.org/wiki/Q185098","display_name":"Electrophoresis","level":2,"score":0.41789793968200684},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.12846916913986206},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.12141549587249756},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.06550824642181396},{"id":"https://openalex.org/C521977710","wikidata":"https://www.wikidata.org/wiki/Q81163","display_name":"Polymer","level":2,"score":0.0540984570980072},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.05394962430000305},{"id":"https://openalex.org/C15920480","wikidata":"https://www.wikidata.org/wiki/Q421281","display_name":"Copolymer","level":3,"score":0.0},{"id":"https://openalex.org/C184651966","wikidata":"https://www.wikidata.org/wiki/Q906356","display_name":"Aqueous solution","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000179","descriptor_name":"Acrylates","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000371","descriptor_name":"Agglutination","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000906","descriptor_name":"Antibodies","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D000917","descriptor_name":"Antibody Formation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000918","descriptor_name":"Antibody Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000936","descriptor_name":"Antigen-Antibody Complex","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D001669","descriptor_name":"Biochemical Phenomena","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001671","descriptor_name":"Biochemistry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003165","descriptor_name":"Complement System Proteins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D003165","descriptor_name":"Complement System Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.4049/jimmunol.106.2.304","doi":"https://doi.org/10.4049/jimmunol.106.2.304","is_oa":false,"landing_page_url":"https://doi.org/10.4049/jimmunol.106.2.304","pdf_url":null,"source":{"id":"https://openalex.org/S38008053","display_name":"The Journal of Immunology","issn_l":"0022-1767","issn":["0022-1767","1550-6606"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4320800377","host_organization_name":"American Association of Immunologists","host_organization_lineage":["https://openalex.org/P4320800377"],"host_organization_lineage_names":["American Association of Immunologists"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Immunology","raw_type":"journal-article"},{"id":"pmid:5545135","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/5545135","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of immunology (Baltimore, Md. : 1950)","raw_type":"Journal Article"},{"id":"mag:1783506013","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/5545135","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1783506013","https://openalex.org/W1603322375","https://openalex.org/W1976870954","https://openalex.org/W2463935919","https://openalex.org/W2021205168","https://openalex.org/W1991337127","https://openalex.org/W1502673207","https://openalex.org/W4313316484","https://openalex.org/W2037664847","https://openalex.org/W1968600111"],"abstract_inverted_index":{"Abstract":[0],"C1q":[1,21,38,62,90,115,146],"has":[2,165],"been":[3,166],"purified":[4,24,37,114],"using":[5,91],"precipitation":[6],"in":[7,43,168],"the":[8,36,44,54],"presence":[9],"of":[10,61,89,110,145],"chelating":[11],"agents":[12],"at":[13],"low":[14],"ionic":[15],"strength.":[16],"The":[17,67,87,112,142],"three":[18],"times":[19],"precipitated":[20],"was":[22,156],"highly":[23,113],"as":[25,147],"shown":[26],"by":[27,149],"immunoelectrophoresis":[28],"and":[29,53,57,73,99,160],"analytic":[30],"acrylamide":[31,120,154],"disc":[32],"electrophoresis.":[33],"On":[34],"immunoelectrophoresis,":[35],"showed":[39,70],"a":[40,48],"single":[41],"line":[42],"slow":[45],"\u03b3-region":[46],"against":[47],"potent":[49,127],"anti-whole":[50],"human":[51],"antiserum,":[52],"hemolytic":[55,71],"activity":[56,60,72,76],"latex":[58,74],"agglutination":[59,75],"coincided":[63],"with":[64],"this":[65],"region.":[66],"final":[68],"product":[69],"but":[77,139],"did":[78],"not":[79,140],"show":[80],"any":[81],"detectable":[82],"C1r":[83],"or":[84],"C1s":[85],"activity.":[86],"yield":[88],"these":[92],"methods":[93],"is":[94],"about":[95,101],"40":[96],"to":[97,162],"60%":[98],"thus":[100],"5":[102],"mg":[103],"can":[104],"be":[105],"obtained":[106,116],"from":[107,117],"100":[108],"ml":[109],"serum.":[111],"3.75%":[118],"preparative":[119],"gels":[121,155,169],"containing":[122,170],"sodium":[123],"dodecyl":[124],"sulfate":[125],"yields":[126],"monospecific":[128],"antisera":[129,135],"when":[130],"injected":[131],"into":[132],"rabbits.":[133],"These":[134],"agglutinate":[136],"EAC1q":[137],"cells":[138],"EA.":[141],"molecular":[143],"weight":[144],"estimated":[148],"its":[150],"relative":[151],"mobility":[152],"on":[153],"387,600":[157],"\u00b1":[158],"10,790,":[159],"breakdown":[161],"smaller":[163],"subunits":[164],"demonstrated":[167],"urea.":[171]},"counts_by_year":[{"year":2019,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2023-01-06T00:00:00"}
