{"id":"https://openalex.org/W4312117404","doi":"https://doi.org/10.1002/yea.3835","title":"Ger1 is a secreted aspartic acid protease essential for spore germination in Ustilago maydis","display_name":"Ger1 is a secreted aspartic acid protease essential for spore germination in Ustilago maydis","publication_year":2022,"publication_date":"2022-12-23","ids":{"openalex":"https://openalex.org/W4312117404","doi":"https://doi.org/10.1002/yea.3835","pmid":"https://pubmed.ncbi.nlm.nih.gov/36562128"},"language":"en","primary_location":{"id":"doi:10.1002/yea.3835","doi":"https://doi.org/10.1002/yea.3835","is_oa":false,"landing_page_url":"https://doi.org/10.1002/yea.3835","pdf_url":null,"source":{"id":"https://openalex.org/S80252601","display_name":"Yeast","issn_l":"0749-503X","issn":["0749-503X","1097-0061"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Yeast","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5058922340","display_name":"Subhasish Mukherjee","orcid":"https://orcid.org/0000-0001-7268-9079","observed_orcids":["https://orcid.org/0000-0001-7268-9079"]},"institutions":[{"id":"https://openalex.org/I152754861","display_name":"Bose Institute","ror":"https://ror.org/01a5mqy88","country_code":"IN","type":"facility","lineage":["https://openalex.org/I152754861"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Subhasish Mukherjee","raw_affiliation_strings":["Division of Plant Biology, Bose Institute, Kolkata, India"],"raw_orcid":"https://orcid.org/0000-0001-7268-9079","affiliations":[{"raw_affiliation_string":"Division of Plant Biology, Bose Institute, Kolkata, India","institution_ids":["https://openalex.org/I152754861"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5077184319","display_name":"Koustav Bhakta","orcid":"https://orcid.org/0000-0002-2180-015X","observed_orcids":["https://orcid.org/0000-0002-2180-015X"]},"institutions":[{"id":"https://openalex.org/I152754861","display_name":"Bose Institute","ror":"https://ror.org/01a5mqy88","country_code":"IN","type":"facility","lineage":["https://openalex.org/I152754861"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Koustav Bhakta","raw_affiliation_strings":["Department of Biochemistry, Bose Institute, Kolkata, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Bose Institute, Kolkata, India","institution_ids":["https://openalex.org/I152754861"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5020740373","display_name":"Abhrajyoti Ghosh","orcid":"https://orcid.org/0000-0002-2469-3740","observed_orcids":["https://orcid.org/0000-0002-2469-3740"]},"institutions":[{"id":"https://openalex.org/I152754861","display_name":"Bose Institute","ror":"https://ror.org/01a5mqy88","country_code":"IN","type":"facility","lineage":["https://openalex.org/I152754861"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Abhrajyoti Ghosh","raw_affiliation_strings":["Department of Biochemistry, Bose Institute, Kolkata, India","Division of Plant Biology Bose Institute Kolkata India"],"raw_orcid":"https://orcid.org/0000-0002-2469-3740","affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Bose Institute, Kolkata, India","institution_ids":["https://openalex.org/I152754861"]},{"raw_affiliation_string":"Division of Plant Biology Bose Institute Kolkata India","institution_ids":["https://openalex.org/I152754861"]}]},{"author_position":"last","author":{"id":null,"display_name":"Anupama Ghosh","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I152754861","display_name":"Bose Institute","ror":"https://ror.org/01a5mqy88","country_code":"IN","type":"facility","lineage":["https://openalex.org/I152754861"]}],"countries":["IN"],"is_corresponding":true,"raw_author_name":"Anupama Ghosh","raw_affiliation_strings":["Department of Biochemistry Bose Institute Kolkata India","Division of Plant Biology, Bose Institute, Kolkata, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry Bose Institute Kolkata India","institution_ids":["https://openalex.org/I152754861"]},{"raw_affiliation_string":"Division of Plant Biology, Bose Institute, Kolkata, India","institution_ids":["https://openalex.org/I152754861"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I152754861"],"apc_list":{"value":3550,"currency":"USD","value_usd":3550},"apc_paid":null,"fwci":0.1303,"has_fulltext":false,"cited_by_count":4,"citation_normalized_percentile":{"value":0.34903205,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"40","issue":"2","first_page":"102","last_page":"116"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10825","display_name":"Plant Pathogens and Fungal Diseases","score":0.6563000082969666,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10825","display_name":"Plant Pathogens and Fungal Diseases","score":0.6563000082969666,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.26589998602867126,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12858","display_name":"Plant Disease Resistance and Genetics","score":0.010499999858438969,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/ustilago-maydis","display_name":"Ustilago maydis","score":0.9269999861717224},{"id":"https://openalex.org/keywords/aspartic-protease","display_name":"aspartic protease","score":0.8059999942779541},{"id":"https://openalex.org/keywords/extracellular-protease","display_name":"extracellular protease","score":0.847000002861023},{"id":"https://openalex.org/keywords/spore-germination","display_name":"spore germination","score":0.9909999966621399},{"id":"https://openalex.org/keywords/sporulation","display_name":"sporulation","score":0.9950000047683716},{"id":"https://openalex.org/keywords/fungal-pathogenicity","display_name":"fungal pathogenicity","score":0.8579999804496765},{"id":"https://openalex.org/keywords/maize-smut-fungus","display_name":"maize smut fungus","score":0.4180000126361847}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.9141112565994263},{"id":"https://openalex.org/C2781222273","wikidata":"https://www.wikidata.org/wiki/Q2361375","display_name":"Ustilago","level":3,"score":0.8875184655189514},{"id":"https://openalex.org/C182220744","wikidata":"https://www.wikidata.org/wiki/Q7251226","display_name":"Proteases","level":3,"score":0.7850531339645386},{"id":"https://openalex.org/C2776714187","wikidata":"https://www.wikidata.org/wiki/Q212410","display_name":"Protease","level":3,"score":0.7074323296546936},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.6688905954360962},{"id":"https://openalex.org/C60987743","wikidata":"https://www.wikidata.org/wiki/Q1460232","display_name":"Virulence","level":3,"score":0.6281713247299194},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.531139075756073},{"id":"https://openalex.org/C2777807008","wikidata":"https://www.wikidata.org/wiki/Q87324999","display_name":"Serine protease","level":4,"score":0.5286688804626465},{"id":"https://openalex.org/C2776460866","wikidata":"https://www.wikidata.org/wiki/Q170065","display_name":"Pathogen","level":2,"score":0.4510495066642761},{"id":"https://openalex.org/C28406088","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Extracellular","level":2,"score":0.42986568808555603},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3099520802497864},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.19178107380867004},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.12263694405555725}],"mesh":[{"descriptor_ui":"D001487","descriptor_name":"Basidiomycota","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D005656","descriptor_name":"Fungal Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D005656","descriptor_name":"Fungal Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013170","descriptor_name":"Spores","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014588","descriptor_name":"Ustilago","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D014588","descriptor_name":"Ustilago","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D057055","descriptor_name":"Aspartic Acid Proteases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D057055","descriptor_name":"Aspartic Acid Proteases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1002/yea.3835","doi":"https://doi.org/10.1002/yea.3835","is_oa":false,"landing_page_url":"https://doi.org/10.1002/yea.3835","pdf_url":null,"source":{"id":"https://openalex.org/S80252601","display_name":"Yeast","issn_l":"0749-503X","issn":["0749-503X","1097-0061"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Yeast","raw_type":"journal-article"},{"id":"pmid:36562128","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/36562128","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Yeast (Chichester, England)","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320320767","display_name":"University Grants Commission","ror":"https://ror.org/04p800546"},{"id":"https://openalex.org/F4320334771","display_name":"Science and Engineering Research Board","ror":"https://ror.org/03ffdsr55"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":43,"referenced_works":["https://openalex.org/W30253000","https://openalex.org/W1534406401","https://openalex.org/W1590341185","https://openalex.org/W1749972705","https://openalex.org/W1930832074","https://openalex.org/W1967690180","https://openalex.org/W1970770879","https://openalex.org/W1999241246","https://openalex.org/W2006650772","https://openalex.org/W2007497503","https://openalex.org/W2026405777","https://openalex.org/W2026952569","https://openalex.org/W2030103136","https://openalex.org/W2030966943","https://openalex.org/W2034523960","https://openalex.org/W2046075462","https://openalex.org/W2064059613","https://openalex.org/W2089434296","https://openalex.org/W2095952521","https://openalex.org/W2097706568","https://openalex.org/W2106860332","https://openalex.org/W2107277218","https://openalex.org/W2110923094","https://openalex.org/W2112595354","https://openalex.org/W2129499959","https://openalex.org/W2137959581","https://openalex.org/W2151663881","https://openalex.org/W2153401093","https://openalex.org/W2154066368","https://openalex.org/W2162631285","https://openalex.org/W2168420558","https://openalex.org/W2169506254","https://openalex.org/W2473499314","https://openalex.org/W2495905941","https://openalex.org/W2612885746","https://openalex.org/W2756898806","https://openalex.org/W2784397033","https://openalex.org/W2809209752","https://openalex.org/W2913863747","https://openalex.org/W2950907198","https://openalex.org/W3081438447","https://openalex.org/W4248486036","https://openalex.org/W4251751280"],"related_works":["https://openalex.org/W2136741522","https://openalex.org/W7029212711","https://openalex.org/W1930832074","https://openalex.org/W2087117408","https://openalex.org/W2473499314","https://openalex.org/W3137879070","https://openalex.org/W2784235587","https://openalex.org/W2036126879","https://openalex.org/W3194298145","https://openalex.org/W1989847083"],"abstract_inverted_index":{"Ustilago":[0],"maydis":[1,44,170],"expresses":[2],"a":[3],"number":[4],"of":[5,12,29,35,42,59,73,78,85,92,99,105,113,122,133,164],"proteases":[6,14,37],"during":[7,89],"its":[8,159],"pathogenic":[9],"lifecycle.":[10],"Some":[11],"the":[13,27,30,39,56,74,86,100,103,106,116,131,134,138,149],"including":[15],"both":[16],"intracellular":[17],"and":[18,118,136],"extracellular":[19],"ones":[20],"have":[21,45,54],"previously":[22],"been":[23,47],"shown":[24],"to":[25,66,155],"influence":[26,129],"virulence":[28],"pathogen.":[31],"However,":[32],"any":[33,97],"role":[34],"secreted":[36,62,180],"in":[38,102,115],"sporulation":[40,117],"process":[41],"U.":[43,123,169],"not":[46],"explored":[48],"earlier.":[49],"In":[50],"this":[51],"study":[52,146],"we":[53],"investigated":[55],"biological":[57,160],"function":[58],"one":[60],"such":[61],"protease,":[63],"Ger1":[64,114,125,154,177],"belonging":[65],"aspartic":[67],"protease":[68,150],"A1":[69],"family.":[70],"An":[71],"assessment":[72],"real":[75],"time":[76],"expression":[77,84],"ger1":[79,139],"revealed":[80,110,175],"an":[81,111],"infection":[82],"specific":[83],"protein":[87,101],"especially":[88],"late":[90],"phases":[91],"infection.":[93],"We":[94],"also":[95,126,147,174],"evaluated":[96],"contribution":[98],"pathogenicity":[104,132],"fungus.":[107],"Our":[108],"data":[109],"involvement":[112],"spore":[119],"germination":[120],"processes":[121],"maydis.":[124],"showed":[127],"positive":[128],"on":[130],"fungus":[135],"accordingly":[137],"deletion":[140],"mutant":[141],"exhibited":[142],"reduced":[143],"pathogenicity.":[144],"The":[145],"demonstrated":[148],"activity":[151],"associated":[152],"with":[153,168],"be":[156],"essential":[157],"for":[158],"function.":[161],"Fluorescence":[162],"microscopy":[163],"maize":[165,182],"plants":[166],"infected":[167],"cells":[171],"expressing":[172],"Ger1-mcherry-HA":[173],"that":[176],"is":[178],"efficiently":[179],"within":[181],"apoplast.":[183]},"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2024,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2023-01-04T00:00:00"}