{"id":"https://openalex.org/W4309809681","doi":"https://doi.org/10.1021/acs.analchem.2c03164","title":"High-Throughput Steady-State Enzyme Kinetics Measured in a Parallel Droplet Generation and Absorbance Detection Platform","display_name":"High-Throughput Steady-State Enzyme Kinetics Measured in a Parallel Droplet Generation and Absorbance Detection Platform","publication_year":2022,"publication_date":"2022-11-23","ids":{"openalex":"https://openalex.org/W4309809681","doi":"https://doi.org/10.1021/acs.analchem.2c03164","pmid":"https://pubmed.ncbi.nlm.nih.gov/36417687","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/9730296"},"language":"en","primary_location":{"id":"doi:10.1021/acs.analchem.2c03164","doi":"https://doi.org/10.1021/acs.analchem.2c03164","is_oa":true,"landing_page_url":"https://doi.org/10.1021/acs.analchem.2c03164","pdf_url":null,"source":{"id":"https://openalex.org/S191963794","display_name":"Analytical Chemistry","issn_l":"0003-2700","issn":["0003-2700","1520-6882"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","datacite","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1021/acs.analchem.2c03164","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5068540457","display_name":"Stefanie Neun","orcid":"https://orcid.org/0000-0002-2469-6984","observed_orcids":["https://orcid.org/0000-0002-2469-6984"]},"institutions":[{"id":"https://openalex.org/I241749","display_name":"University of Cambridge","ror":"https://ror.org/013meh722","country_code":"GB","type":"education","lineage":["https://openalex.org/I241749"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Stefanie Neun","raw_affiliation_strings":["Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, U.K"],"raw_orcid":"https://orcid.org/0000-0002-2469-6984","affiliations":[{"raw_affiliation_string":"Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, U.K","institution_ids":["https://openalex.org/I241749"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5036202764","display_name":"Liisa van Vliet","orcid":"https://orcid.org/0000-0002-5196-7073","observed_orcids":["https://orcid.org/0000-0002-5196-7073"]},"institutions":[{"id":"https://openalex.org/I241749","display_name":"University of Cambridge","ror":"https://ror.org/013meh722","country_code":"GB","type":"education","lineage":["https://openalex.org/I241749"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Liisa van Vliet","raw_affiliation_strings":["Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, U.K"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, U.K","institution_ids":["https://openalex.org/I241749"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5051612157","display_name":"Florian Hollfelder","orcid":"https://orcid.org/0000-0002-1367-6312","observed_orcids":["https://orcid.org/0000-0002-1367-6312","https://orcid.org/0000-0001-7694-7805"]},"institutions":[{"id":"https://openalex.org/I241749","display_name":"University of Cambridge","ror":"https://ror.org/013meh722","country_code":"GB","type":"education","lineage":["https://openalex.org/I241749"]}],"countries":["GB"],"is_corresponding":true,"raw_author_name":"Florian Hollfelder","raw_affiliation_strings":["Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, U.K"],"raw_orcid":"https://orcid.org/0000-0002-1367-6312","affiliations":[{"raw_affiliation_string":"Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, U.K","institution_ids":["https://openalex.org/I241749"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5039098154","display_name":"Fabrice Gielen","orcid":"https://orcid.org/0000-0003-0604-7224","observed_orcids":["https://orcid.org/0000-0003-0604-7224"]},"institutions":[{"id":"https://openalex.org/I23923803","display_name":"University of Exeter","ror":"https://ror.org/03yghzc09","country_code":"GB","type":"education","lineage":["https://openalex.org/I23923803"]}],"countries":["GB"],"is_corresponding":true,"raw_author_name":"Fabrice Gielen","raw_affiliation_strings":["Living Systems Institute and College of Engineering Mathematics and Physical Sciences, University of Exeter, Exeter EX4 4QD, U.K"],"raw_orcid":"https://orcid.org/0000-0003-0604-7224","affiliations":[{"raw_affiliation_string":"Living Systems Institute and College of Engineering Mathematics and Physical Sciences, University of Exeter, Exeter EX4 4QD, U.K","institution_ids":["https://openalex.org/I23923803"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5039098154","https://openalex.org/A5051612157"],"corresponding_institution_ids":["https://openalex.org/I23923803","https://openalex.org/I241749"],"apc_list":{"value":5000,"currency":"USD","value_usd":5000},"apc_paid":{"value":5000,"currency":"USD","value_usd":5000},"fwci":0.9674,"has_fulltext":false,"cited_by_count":18,"citation_normalized_percentile":{"value":0.68127555,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":95,"max":98},"biblio":{"volume":"94","issue":"48","first_page":"16701","last_page":"16710"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11407","display_name":"Innovative Microfluidic and Catalytic Techniques Innovation","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1507","display_name":"Fluid Flow and Transfer Processes"},"field":{"id":"https://openalex.org/fields/15","display_name":"Chemical Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11407","display_name":"Innovative Microfluidic and Catalytic Techniques Innovation","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1507","display_name":"Fluid Flow and Transfer Processes"},"field":{"id":"https://openalex.org/fields/15","display_name":"Chemical Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10412","display_name":"Microfluidic and Capillary Electrophoresis Applications","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/droplet-microfluidics","display_name":"droplet microfluidics","score":0.7440000176429749},{"id":"https://openalex.org/keywords/water-in-oil-emulsion-droplets","display_name":"water-in-oil emulsion droplets","score":0.9139999747276306},{"id":"https://openalex.org/keywords/michaelis-menten-kinetics","display_name":"Michaelis-Menten kinetics","score":0.9829999804496765},{"id":"https://openalex.org/keywords/steady-state-kinetics","display_name":"steady-state kinetics","score":0.6809999942779541},{"id":"https://openalex.org/keywords/glycosidase-activity","display_name":"glycosidase activity","score":0.6269999742507935},{"id":"https://openalex.org/keywords/enzymatic-hydrolysis","display_name":"enzymatic hydrolysis","score":0.7689999938011169},{"id":"https://openalex.org/keywords/line-scan-camera","display_name":"line-scan camera","score":0.5519999861717224}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.970228910446167},{"id":"https://openalex.org/C98015330","wikidata":"https://www.wikidata.org/wiki/Q907315","display_name":"Absorbance","level":2,"score":0.8056965470314026},{"id":"https://openalex.org/C148898269","wikidata":"https://www.wikidata.org/wiki/Q1108792","display_name":"Kinetics","level":2,"score":0.7076476216316223},{"id":"https://openalex.org/C8171440","wikidata":"https://www.wikidata.org/wiki/Q903414","display_name":"Steady state (chemistry)","level":2,"score":0.651546061038971},{"id":"https://openalex.org/C157764524","wikidata":"https://www.wikidata.org/wiki/Q1383412","display_name":"Throughput","level":3,"score":0.6119301319122314},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.4715167284011841},{"id":"https://openalex.org/C56856141","wikidata":"https://www.wikidata.org/wiki/Q883112","display_name":"Enzyme kinetics","level":4,"score":0.4459731876850128},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.364875465631485},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.16334611177444458},{"id":"https://openalex.org/C147789679","wikidata":"https://www.wikidata.org/wiki/Q11372","display_name":"Physical chemistry","level":1,"score":0.16219410300254822},{"id":"https://openalex.org/C74650414","wikidata":"https://www.wikidata.org/wiki/Q11397","display_name":"Classical mechanics","level":1,"score":0.05191493034362793},{"id":"https://openalex.org/C76155785","wikidata":"https://www.wikidata.org/wiki/Q418","display_name":"Telecommunications","level":1,"score":0.0},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C41183919","wikidata":"https://www.wikidata.org/wiki/Q423026","display_name":"Active site","level":3,"score":0.0},{"id":"https://openalex.org/C555944384","wikidata":"https://www.wikidata.org/wiki/Q249","display_name":"Wireless","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D001681","descriptor_name":"Biological Assay","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004655","descriptor_name":"Emulsions","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006868","descriptor_name":"Hydrolysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D044085","descriptor_name":"Microfluidics","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D046210","descriptor_name":"Microfluidic Analytical Techniques","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":10,"locations":[{"id":"doi:10.1021/acs.analchem.2c03164","doi":"https://doi.org/10.1021/acs.analchem.2c03164","is_oa":true,"landing_page_url":"https://doi.org/10.1021/acs.analchem.2c03164","pdf_url":null,"source":{"id":"https://openalex.org/S191963794","display_name":"Analytical Chemistry","issn_l":"0003-2700","issn":["0003-2700","1520-6882"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Chemistry","raw_type":"journal-article"},{"id":"doi:10.1101/2022.07.28.500969","doi":"https://doi.org/10.1101/2022.07.28.500969","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2022.07.28.500969","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2022/07/31/2022.07.28.500969.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},{"id":"pmid:36417687","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/36417687","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical chemistry","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:9730296","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC9730296/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Anal Chem","raw_type":"Text"},{"id":"pmh:oai:figshare.com:article/29793785","doi":null,"is_oa":true,"landing_page_url":"https://figshare.com/articles/journal_contribution/High-throughput_steady-state_enzyme_kinetics_measured_in_a_parallel_droplet_generation_and_absorbance_detection_platform_/29793785","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:ore.exeter.ac.uk:10871/132386","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10871/132386","pdf_url":null,"source":{"id":"https://openalex.org/S4306401998","display_name":"Open Research Exeter (University of Exeter)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I23923803","host_organization_name":"University of Exeter","host_organization_lineage":["https://openalex.org/I23923803"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Article"},{"id":"pmh:oai:www.repository.cam.ac.uk:1810/344091","doi":null,"is_oa":true,"landing_page_url":"https://www.repository.cam.ac.uk/handle/1810/344091","pdf_url":null,"source":{"id":"https://openalex.org/S4306401777","display_name":"Apollo (University of Cambridge)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I241749","host_organization_name":"University of Cambridge","host_organization_lineage":["https://openalex.org/I241749"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Article"},{"id":"pmh:oai:www.repository.cam.ac.uk:1810/344769","doi":null,"is_oa":true,"landing_page_url":"https://www.repository.cam.ac.uk/handle/1810/344769","pdf_url":null,"source":{"id":"https://openalex.org/S4306401777","display_name":"Apollo (University of Cambridge)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I241749","host_organization_name":"University of Cambridge","host_organization_lineage":["https://openalex.org/I241749"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"nlmid: 0370536","raw_type":"Article"},{"id":"doi:10.17863/cam.91515","doi":"https://doi.org/10.17863/cam.91515","is_oa":true,"landing_page_url":"https://doi.org/10.17863/cam.91515","pdf_url":null,"source":{"id":"https://openalex.org/S7407050737","display_name":"Apollo","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"JournalArticle"},{"id":"doi:10.17863/cam.92192","doi":"https://doi.org/10.17863/cam.92192","is_oa":true,"landing_page_url":"https://doi.org/10.17863/cam.92192","pdf_url":null,"source":{"id":"https://openalex.org/S7407050737","display_name":"Apollo","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"JournalArticle"}],"best_oa_location":{"id":"doi:10.1021/acs.analchem.2c03164","doi":"https://doi.org/10.1021/acs.analchem.2c03164","is_oa":true,"landing_page_url":"https://doi.org/10.1021/acs.analchem.2c03164","pdf_url":null,"source":{"id":"https://openalex.org/S191963794","display_name":"Analytical Chemistry","issn_l":"0003-2700","issn":["0003-2700","1520-6882"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1147893575","display_name":null,"funder_award_id":"EU H2020","funder_id":"https://openalex.org/F4320334627","funder_display_name":"Engineering and Physical Sciences Research Council"},{"id":"https://openalex.org/G187865429","display_name":null,"funder_award_id":"695669","funder_id":"https://openalex.org/F4320332999","funder_display_name":"Horizon 2020 Framework Programme"},{"id":"https://openalex.org/G3843748092","display_name":"SENSE - Screening of ENvironmental SEquences to discover novel protein functions using informatics target selection and high-throughput validation","funder_award_id":"BB/T003545/1","funder_id":"https://openalex.org/F4320334629","funder_display_name":"Biotechnology and Biological Sciences Research Council"},{"id":"https://openalex.org/G4326476702","display_name":"Exploring the Chemical Biology of Sequence Space via Picoliter Droplets","funder_award_id":"695669","funder_id":"https://openalex.org/F4320338335","funder_display_name":"H2020 European Research Council"},{"id":"https://openalex.org/G4742809182","display_name":null,"funder_award_id":"685474","funder_id":"https://openalex.org/F4320332999","funder_display_name":"Horizon 2020 Framework Programme"},{"id":"https://openalex.org/G495187929","display_name":null,"funder_award_id":"EU\u2010H2020","funder_id":"https://openalex.org/F4320332999","funder_display_name":"Horizon 2020 Framework Programme"},{"id":"https://openalex.org/G5045220161","display_name":null,"funder_award_id":"H2020","funder_id":"https://openalex.org/F4320334627","funder_display_name":"Engineering and Physical Sciences Research Council"},{"id":"https://openalex.org/G6054346117","display_name":"Microdroplet technology - the next stage","funder_award_id":"EP/H046593/1","funder_id":"https://openalex.org/F4320334627","funder_display_name":"Engineering and Physical Sciences Research Council"},{"id":"https://openalex.org/G8404395069","display_name":"Advanced toolbox for rapid and cost-effective functional metagenomic screening - microbiology meets microfluidics.","funder_award_id":"685474","funder_id":"https://openalex.org/F4320338482","funder_display_name":"H2020 LEIT Biotechnology"},{"id":"https://openalex.org/G8684053183","display_name":"RAPID DISCOVERY AND DEVELOPMENT OF ENZYMES FOR NOVEL AND GREENER CONSUMER PRODUCTS","funder_award_id":"101000560","funder_id":"https://openalex.org/F4320338343","funder_display_name":"H2020 Food"}],"funders":[{"id":"https://openalex.org/F4320307770","display_name":"AstraZeneca","ror":"https://ror.org/04r9x1a08"},{"id":"https://openalex.org/F4320332167","display_name":"Directorate for Biological Sciences","ror":"https://ror.org/001xhss06"},{"id":"https://openalex.org/F4320332999","display_name":"Horizon 2020 Framework Programme","ror":"https://ror.org/00k4n6c32"},{"id":"https://openalex.org/F4320334627","display_name":"Engineering and Physical Sciences Research Council","ror":"https://ror.org/0439y7842"},{"id":"https://openalex.org/F4320334629","display_name":"Biotechnology and Biological Sciences Research Council","ror":"https://ror.org/00cwqg982"},{"id":"https://openalex.org/F4320338335","display_name":"H2020 European Research Council","ror":"https://ror.org/0472cxd90"},{"id":"https://openalex.org/F4320338343","display_name":"H2020 Food","ror":null},{"id":"https://openalex.org/F4320338482","display_name":"H2020 LEIT Biotechnology","ror":null}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4309809681.pdf","grobid_xml":"https://content.openalex.org/works/W4309809681.grobid-xml"},"referenced_works_count":47,"referenced_works":["https://openalex.org/W1945992259","https://openalex.org/W1993649991","https://openalex.org/W2023090507","https://openalex.org/W2025565063","https://openalex.org/W2028894110","https://openalex.org/W2030300881","https://openalex.org/W2041677432","https://openalex.org/W2041881366","https://openalex.org/W2054156743","https://openalex.org/W2055041227","https://openalex.org/W2065093209","https://openalex.org/W2067315058","https://openalex.org/W2113601822","https://openalex.org/W2113606383","https://openalex.org/W2115026474","https://openalex.org/W2151100442","https://openalex.org/W2151457629","https://openalex.org/W2201645878","https://openalex.org/W2210738511","https://openalex.org/W2253102388","https://openalex.org/W2301722460","https://openalex.org/W2320714052","https://openalex.org/W2321519616","https://openalex.org/W2326684736","https://openalex.org/W2338219931","https://openalex.org/W2555293666","https://openalex.org/W2562056474","https://openalex.org/W2754724529","https://openalex.org/W2765974283","https://openalex.org/W2790084197","https://openalex.org/W2883661408","https://openalex.org/W2916179393","https://openalex.org/W2953483200","https://openalex.org/W2967686740","https://openalex.org/W2984894304","https://openalex.org/W2996030271","https://openalex.org/W3001972792","https://openalex.org/W3045431364","https://openalex.org/W3096739358","https://openalex.org/W3108171657","https://openalex.org/W3112376646","https://openalex.org/W3124859893","https://openalex.org/W3135055844","https://openalex.org/W3164793303","https://openalex.org/W3177828909","https://openalex.org/W3203729472","https://openalex.org/W4284666963"],"related_works":["https://openalex.org/W2055041227","https://openalex.org/W2995499826","https://openalex.org/W3138375112","https://openalex.org/W4407637439","https://openalex.org/W1533016923","https://openalex.org/W2025565063","https://openalex.org/W2338675709","https://openalex.org/W4408849872","https://openalex.org/W2301722460","https://openalex.org/W1597970980"],"abstract_inverted_index":{"ABSTRACT":[0],"Microfluidic":[1],"water-in-oil":[2],"emulsion":[3,39],"droplets":[4,40,108,142,175],"are":[5,41,59,165],"becoming":[6],"a":[7,77,89,144,182,191,206,232,272],"mainstay":[8],"of":[9,53,70,107,115,123,172,184,212,237,240,262,279],"experimental":[10],"biology,":[11],"where":[12],"they":[13],"replace":[14],"the":[15,28,121,124,133,141,196,223],"classical":[16],"test":[17],"tube.":[18],"In":[19,205],"most":[20],"applications":[21],"(e.g.":[22,52],"in":[23,234,242],"ultrahigh":[24],"throughput":[25,183],"directed":[26],"evolution)":[27],"droplet":[29,79,86,125],"content":[30],"is":[31,72,117,203],"identical":[32],"for":[33,43,56,109,167,225,228,276],"all":[34],"compartmentalized":[35],"assay":[36],"reactions.":[37],"When":[38],"used":[42],"kinetics":[44,164,218],"or":[45],"other":[46],"functional":[47],"assays,":[48],"though,":[49],"concentration":[50,102,169],"dependencies":[51],"initial":[54],"rates":[55],"Michaelis-Menten":[57,217],"plots)":[58],"required.":[60],"Droplet-on-demand":[61],"systems":[62],"satisfy":[63],"this":[64,267],"need":[65],"but":[66],"extracting":[67],"large":[68],"amounts":[69],"data":[71,187,254],"challenging.":[73],"Here":[74],"we":[75],"introduce":[76],"multiplexed":[78],"absorbance":[80,122,153],"detector":[81],"which,":[82],"coupled":[83],"to":[84,94,159,195,221,275],"semi-automated":[85],"generation,":[87],"forms":[88],"tubing-based":[90],"droplet-on-demand":[91],"system":[92,268],"able":[93],"generate":[95],"and":[96,162,214],"extract":[97],"quantitative":[98],"datasets":[99,211],"from":[100,283],"defined":[101],"gradients":[103,170],"across":[104],"multiple":[105,110,148],"series":[106],"time":[111,130],"points.":[112],"The":[113,248],"emergence":[114],"product":[116],"detected":[118],"by":[119],"reading":[120],"sets":[126],"at":[127,155],"multiple,":[128],"adjustable":[129],"points":[131,188],"(reversing":[132],"flow":[134],"direction":[135],"after":[136],"each":[137],"detection,":[138],"so":[139],"that":[140,259,266],"pass":[143],"line":[145],"scan":[146],"camera":[147],"times).":[149],"Detection":[150],"multiplexing":[151],"allows":[152,260],"values":[154],"twelve":[156,209],"distinct":[157],"positions":[158],"be":[160],"measured":[161],"enzyme":[163,226],"recorded":[166],"label-free":[168],"(composed":[171],"about":[173],"60":[174],"each,":[176],"covering":[177,231],"as":[178,271],"many":[179],"concentrations).":[180],"With":[181],"around":[185],"8640":[186],"per":[189],"hour,":[190],"10-fold":[192],"improvement":[193],"compared":[194],"previously":[197],"reported":[198],"single":[199,207],"point":[200],"detection":[201],"method":[202],"achieved.":[204],"experiment,":[208],"full":[210],"high-resolution":[213],"high":[215,251],"accuracy":[216],"were":[219],"determined":[220],"demonstrate":[222],"potential":[224],"characterization":[227],"glycosidase":[229],"substrates":[230],"range":[233,258],"enzymatic":[235],"hydrolysis":[236],"seven":[238],"orders":[239],"magnitude":[241],"k":[243],"cat":[244],"/K":[245],"M":[246],".":[247],"straightforward":[249],"set-up,":[250],"throughput,":[252],"excellent":[253],"quality,":[255],"wide":[256],"dynamic":[257],"coverage":[261],"diverse":[263],"activities":[264],"suggest":[265],"may":[269],"serve":[270],"miniaturized":[273],"spectrophotometer":[274],"detailed":[277],"analysis":[278],"study":[280],"clones":[281],"emerging":[282],"large-scale":[284],"combinatorial":[285],"experiments.":[286],"Abstract":[287],"Figure":[288]},"counts_by_year":[{"year":2026,"cited_by_count":5},{"year":2025,"cited_by_count":6},{"year":2024,"cited_by_count":4},{"year":2023,"cited_by_count":3}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2022-11-29T00:00:00"}