{"id":"https://openalex.org/W4308997809","doi":"https://doi.org/10.1016/j.mtbio.2022.100492","title":"Recombinant protein condensation inside E. coli enables the development of building blocks for bioinspired materials engineering \u2013 Biomimetic spider silk protein as a case study","display_name":"Recombinant protein condensation inside E. coli enables the development of building blocks for bioinspired materials engineering \u2013 Biomimetic spider silk protein as a case study","publication_year":2022,"publication_date":"2022-11-14","ids":{"openalex":"https://openalex.org/W4308997809","doi":"https://doi.org/10.1016/j.mtbio.2022.100492","pmid":"https://pubmed.ncbi.nlm.nih.gov/36420055","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/9677211"},"language":"en","primary_location":{"id":"doi:10.1016/j.mtbio.2022.100492","doi":"https://doi.org/10.1016/j.mtbio.2022.100492","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.mtbio.2022.100492","pdf_url":"https://www.sciencedirect.com/science/article/pii/S2590006422002903/pdf","source":{"id":"https://openalex.org/S4210219546","display_name":"Materials Today Bio","issn_l":"2590-0064","issn":["2590-0064"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-d","jufo-1","ki-jl-1","norway-1","oarl","poland-20"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Materials Today Bio","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.sciencedirect.com/science/article/pii/S2590006422002903/pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5029400426","display_name":"Bartosz Gabryelczyk","orcid":"https://orcid.org/0000-0002-8150-8353","observed_orcids":["https://orcid.org/0000-0002-8150-8353"]},"institutions":[{"id":"https://openalex.org/I9927081","display_name":"Aalto University","ror":"https://ror.org/020hwjq30","country_code":"FI","type":"education","lineage":["https://openalex.org/I9927081"]}],"countries":["FI"],"is_corresponding":true,"raw_author_name":"Bartosz Gabryelczyk","raw_affiliation_strings":["Department of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, Espoo, Finland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, Espoo, Finland","institution_ids":["https://openalex.org/I9927081"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066856079","display_name":"Fred\u2010Eric Sammalisto","orcid":"https://orcid.org/0000-0002-8021-9867","observed_orcids":["https://orcid.org/0000-0002-8021-9867"]},"institutions":[{"id":"https://openalex.org/I9927081","display_name":"Aalto University","ror":"https://ror.org/020hwjq30","country_code":"FI","type":"education","lineage":["https://openalex.org/I9927081"]}],"countries":["FI"],"is_corresponding":false,"raw_author_name":"Fred-Eric Sammalisto","raw_affiliation_strings":["Department of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, Espoo, Finland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, Espoo, Finland","institution_ids":["https://openalex.org/I9927081"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5034000451","display_name":"Julie\u2010Anne Gandier","orcid":"https://orcid.org/0000-0001-8164-8543","observed_orcids":["https://orcid.org/0000-0001-8164-8543"]},"institutions":[{"id":"https://openalex.org/I9927081","display_name":"Aalto University","ror":"https://ror.org/020hwjq30","country_code":"FI","type":"education","lineage":["https://openalex.org/I9927081"]}],"countries":["FI"],"is_corresponding":false,"raw_author_name":"Julie-Anne Gandier","raw_affiliation_strings":["Department of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, Espoo, Finland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, Espoo, Finland","institution_ids":["https://openalex.org/I9927081"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5055284430","display_name":"Jianhui Feng","orcid":"https://orcid.org/0000-0001-6372-6245","observed_orcids":["https://orcid.org/0000-0001-6372-6245"]},"institutions":[{"id":"https://openalex.org/I9927081","display_name":"Aalto University","ror":"https://ror.org/020hwjq30","country_code":"FI","type":"education","lineage":["https://openalex.org/I9927081"]}],"countries":["FI"],"is_corresponding":false,"raw_author_name":"Jianhui Feng","raw_affiliation_strings":["Department of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, Espoo, Finland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, Espoo, Finland","institution_ids":["https://openalex.org/I9927081"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5027423638","display_name":"Gr\u00e9gory Beaune","orcid":"https://orcid.org/0000-0003-2068-0499","observed_orcids":["https://orcid.org/0000-0003-2068-0499"]},"institutions":[{"id":"https://openalex.org/I9927081","display_name":"Aalto University","ror":"https://ror.org/020hwjq30","country_code":"FI","type":"education","lineage":["https://openalex.org/I9927081"]}],"countries":["FI"],"is_corresponding":false,"raw_author_name":"Gr\u00e9gory Beaune","raw_affiliation_strings":["Department of Applied Physics, School of Science, Aalto University, Espoo, Finland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Applied Physics, School of Science, Aalto University, Espoo, Finland","institution_ids":["https://openalex.org/I9927081"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5028525678","display_name":"Jaakko V. I. Timonen","orcid":"https://orcid.org/0000-0002-5499-1482","observed_orcids":["https://orcid.org/0000-0002-5499-1482"]},"institutions":[{"id":"https://openalex.org/I9927081","display_name":"Aalto University","ror":"https://ror.org/020hwjq30","country_code":"FI","type":"education","lineage":["https://openalex.org/I9927081"]}],"countries":["FI"],"is_corresponding":false,"raw_author_name":"Jaakko V.I. Timonen","raw_affiliation_strings":["Department of Applied Physics, School of Science, Aalto University, Espoo, Finland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Applied Physics, School of Science, Aalto University, Espoo, Finland","institution_ids":["https://openalex.org/I9927081"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5085039475","display_name":"Markus B. Linder","orcid":"https://orcid.org/0000-0002-7271-6441","observed_orcids":["https://orcid.org/0000-0002-7271-6441"]},"institutions":[{"id":"https://openalex.org/I9927081","display_name":"Aalto University","ror":"https://ror.org/020hwjq30","country_code":"FI","type":"education","lineage":["https://openalex.org/I9927081"]}],"countries":["FI"],"is_corresponding":true,"raw_author_name":"Markus B. Linder","raw_affiliation_strings":["Department of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, Espoo, Finland"],"raw_orcid":"https://orcid.org/0000-0002-7271-6441","affiliations":[{"raw_affiliation_string":"Department of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, Espoo, Finland","institution_ids":["https://openalex.org/I9927081"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5029400426","https://openalex.org/A5085039475"],"corresponding_institution_ids":["https://openalex.org/I9927081"],"apc_list":{"value":3000,"currency":"USD","value_usd":3000},"apc_paid":{"value":2630,"currency":"EUR","value_usd":2836},"fwci":0.7483,"has_fulltext":true,"cited_by_count":19,"citation_normalized_percentile":{"value":0.5683352,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":99},"biblio":{"volume":"17","issue":null,"first_page":"100492","last_page":"100492"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11966","display_name":"Silk-based biomaterials and applications","score":0.9804999828338623,"subfield":{"id":"https://openalex.org/subfields/2502","display_name":"Biomaterials"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11966","display_name":"Silk-based biomaterials and applications","score":0.9804999828338623,"subfield":{"id":"https://openalex.org/subfields/2502","display_name":"Biomaterials"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.01360000018030405,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11419","display_name":"Supramolecular Self-Assembly in Materials","score":0.0006000000284984708,"subfield":{"id":"https://openalex.org/subfields/2502","display_name":"Biomaterials"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/protein-condensates","display_name":"protein condensates","score":0.8560000061988831},{"id":"https://openalex.org/keywords/liquid-liquid-phase-separation","display_name":"liquid-liquid phase separation","score":0.9700000286102295},{"id":"https://openalex.org/keywords/recombinant-protein-overexpression","display_name":"recombinant protein overexpression","score":0.7990000247955322},{"id":"https://openalex.org/keywords/spidroin","display_name":"spidroin","score":0.6990000009536743},{"id":"https://openalex.org/keywords/macromolecular-crowding","display_name":"macromolecular crowding","score":0.8240000009536743},{"id":"https://openalex.org/keywords/biomimetic-materials","display_name":"biomimetic materials","score":0.7369999885559082},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.875}],"concepts":[{"id":"https://openalex.org/C2776847223","wikidata":"https://www.wikidata.org/wiki/Q2740926","display_name":"Spider silk","level":3,"score":0.6955139636993408},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.6442705392837524},{"id":"https://openalex.org/C79879829","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Intracellular","level":2,"score":0.5529138445854187},{"id":"https://openalex.org/C2780431358","wikidata":"https://www.wikidata.org/wiki/Q653622","display_name":"SILK","level":2,"score":0.5524777173995972},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.47618189454078674},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.47208425402641296},{"id":"https://openalex.org/C190062978","wikidata":"https://www.wikidata.org/wiki/Q79899","display_name":"Cytoplasm","level":2,"score":0.45851776003837585},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.44942259788513184},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.3488069474697113},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.34667372703552246},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2799376845359802},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.27513545751571655},{"id":"https://openalex.org/C159985019","wikidata":"https://www.wikidata.org/wiki/Q181790","display_name":"Composite material","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[],"locations_count":6,"locations":[{"id":"doi:10.1016/j.mtbio.2022.100492","doi":"https://doi.org/10.1016/j.mtbio.2022.100492","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.mtbio.2022.100492","pdf_url":"https://www.sciencedirect.com/science/article/pii/S2590006422002903/pdf","source":{"id":"https://openalex.org/S4210219546","display_name":"Materials Today Bio","issn_l":"2590-0064","issn":["2590-0064"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-d","jufo-1","ki-jl-1","norway-1","oarl","poland-20"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Materials Today Bio","raw_type":"journal-article"},{"id":"doi:10.1101/2022.09.14.507915","doi":"https://doi.org/10.1101/2022.09.14.507915","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2022.09.14.507915","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2022/09/15/2022.09.14.507915.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},{"id":"pmid:36420055","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/36420055","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Materials today. Bio","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:9677211","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC9677211/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Mater Today Bio","raw_type":"Text"},{"id":"pmh:oai:aaltodoc.aalto.fi:123456789/118015","doi":null,"is_oa":true,"landing_page_url":"https://research.aalto.fi/en/publications/0ddec577-1792-45ca-a49c-ef2da9d18cba","pdf_url":"https://research.aalto.fi/files/93695198/CHEM_Gabryelczyk_et_al_Recombinant_protein_condensation_2022_Materials_Today_Bio.pdf","source":{"id":"https://openalex.org/S4306401662","display_name":"Aaltodoc (Aalto University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I9927081","host_organization_name":"Aalto University","host_organization_lineage":["https://openalex.org/I9927081"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"info:eu-repo/semantics/article"},{"id":"pmh:oai:doaj.org/article:b4bb0ec1e2224bfd85c7cb46900f9529","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/b4bb0ec1e2224bfd85c7cb46900f9529","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Materials Today Bio, Vol 17, Iss , Pp 100492- (2022)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1016/j.mtbio.2022.100492","doi":"https://doi.org/10.1016/j.mtbio.2022.100492","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.mtbio.2022.100492","pdf_url":"https://www.sciencedirect.com/science/article/pii/S2590006422002903/pdf","source":{"id":"https://openalex.org/S4210219546","display_name":"Materials Today Bio","issn_l":"2590-0064","issn":["2590-0064"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-d","jufo-1","ki-jl-1","norway-1","oarl","poland-20"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Materials Today Bio","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1831555031","display_name":"Center of Excellence in Life-inspired Hybrid Materials","funder_award_id":"346112","funder_id":"https://openalex.org/F4320321108","funder_display_name":"Academy of Finland"},{"id":"https://openalex.org/G3233247360","display_name":null,"funder_award_id":"2022\\u20132029","funder_id":"https://openalex.org/F4320321108","funder_display_name":"Academy of Finland"},{"id":"https://openalex.org/G3603259962","display_name":null,"funder_award_id":"2022-2029","funder_id":"https://openalex.org/F4320321108","funder_display_name":"Academy of Finland"},{"id":"https://openalex.org/G4579084919","display_name":null,"funder_award_id":"0061306","funder_id":"https://openalex.org/F4320325957","funder_display_name":"Novo Nordisk Fonden"},{"id":"https://openalex.org/G4632460807","display_name":null,"funder_award_id":"NNF20OC0061306","funder_id":"https://openalex.org/F4320325957","funder_display_name":"Novo Nordisk Fonden"},{"id":"https://openalex.org/G4669141798","display_name":"Center of Excellence in Life-Inspired Hybrid Materials","funder_award_id":"346105","funder_id":"https://openalex.org/F4320321108","funder_display_name":"Academy of Finland"},{"id":"https://openalex.org/G6553923075","display_name":null,"funder_award_id":"NNF19OC0054862","funder_id":"https://openalex.org/F4320325957","funder_display_name":"Novo Nordisk Fonden"},{"id":"https://openalex.org/G8675886387","display_name":null,"funder_award_id":"315140","funder_id":"https://openalex.org/F4320321108","funder_display_name":"Academy of Finland"}],"funders":[{"id":"https://openalex.org/F4320321108","display_name":"Academy of Finland","ror":"https://ror.org/05k73zm37"},{"id":"https://openalex.org/F4320321394","display_name":"Aalto-Yliopisto","ror":"https://ror.org/020hwjq30"},{"id":"https://openalex.org/F4320322436","display_name":"Novo Nordisk","ror":"https://ror.org/0435rc536"},{"id":"https://openalex.org/F4320325957","display_name":"Novo Nordisk Fonden","ror":"https://ror.org/04txyc737"},{"id":"https://openalex.org/F4320333873","display_name":"Helsinki Institute of Life Science, Helsingin Yliopisto","ror":"https://ror.org/018bh0m68"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4308997809.pdf","grobid_xml":"https://content.openalex.org/works/W4308997809.grobid-xml"},"referenced_works_count":52,"referenced_works":["https://openalex.org/W1913602352","https://openalex.org/W1965108370","https://openalex.org/W1976668599","https://openalex.org/W1982904094","https://openalex.org/W2003258297","https://openalex.org/W2005555532","https://openalex.org/W2006560661","https://openalex.org/W2016667239","https://openalex.org/W2035153699","https://openalex.org/W2059613779","https://openalex.org/W2067550411","https://openalex.org/W2069343079","https://openalex.org/W2080186079","https://openalex.org/W2083238386","https://openalex.org/W2088342906","https://openalex.org/W2088415937","https://openalex.org/W2094861891","https://openalex.org/W2096507065","https://openalex.org/W2099626559","https://openalex.org/W2108073693","https://openalex.org/W2112419909","https://openalex.org/W2128267600","https://openalex.org/W2139066576","https://openalex.org/W2147700328","https://openalex.org/W2163222586","https://openalex.org/W2274641792","https://openalex.org/W2331262552","https://openalex.org/W2467160315","https://openalex.org/W2571023008","https://openalex.org/W2593011934","https://openalex.org/W2611830368","https://openalex.org/W2763440574","https://openalex.org/W2794654572","https://openalex.org/W2807652661","https://openalex.org/W2809941613","https://openalex.org/W2884082692","https://openalex.org/W2914907359","https://openalex.org/W2918586654","https://openalex.org/W3013894338","https://openalex.org/W3027558893","https://openalex.org/W3037436725","https://openalex.org/W3046463281","https://openalex.org/W3049249652","https://openalex.org/W3097240801","https://openalex.org/W3112435700","https://openalex.org/W3120439445","https://openalex.org/W3166344905","https://openalex.org/W3191750976","https://openalex.org/W4220928297","https://openalex.org/W4223610786","https://openalex.org/W4225092890","https://openalex.org/W4229070144"],"related_works":["https://openalex.org/W4402575285","https://openalex.org/W7112787700","https://openalex.org/W4386580479","https://openalex.org/W4389935193","https://openalex.org/W4403448926","https://openalex.org/W7146439661","https://openalex.org/W4406681941","https://openalex.org/W7116428691","https://openalex.org/W7008325823","https://openalex.org/W4409437012"],"abstract_inverted_index":{"Recombinant":[0],"expression":[1,39],"of":[2,17,56,101,126],"proteins":[3,140],"destined":[4],"to":[5,21,34,74,118],"form":[6],"biological":[7],"materials":[8,143],"often":[9],"results":[10],"in":[11,42,50,63,82,91,95,98],"poor":[12],"production":[13],"yields":[14],"or":[15],"loss":[16],"their":[18,83,146],"function":[19],"due":[20],"premature":[22],"aggregation.":[23],"Recently,":[24],"liquid-liquid":[25],"phase":[26],"separation":[27],"has":[28],"been":[29],"proposed":[30],"as":[31,135],"a":[32,75,80,102,113,136],"mechanism":[33],"control":[35],"protein":[36,67,127],"solubility":[37],"during":[38,52,112],"and":[40,111,145],"accumulation":[41],"the":[43,53,57,92,99,107],"cytoplasm.":[44],"Here,":[45],"we":[46,122],"investigate":[47],"this":[48],"process":[49,116],"vivo":[51],"recombinant":[54],"overexpression":[55],"mimetic":[58],"spider":[59],"silk":[60],"mini-spidroin":[61],"NT2RepCT":[62],"Escherichia":[64],"coli.":[65],"The":[66],"forms":[68],"intracellular":[69,109],"liquid-like":[70],"condensates":[71,128],"that":[72,124],"shift":[73],"solid-like":[76],"state":[77],"triggered":[78],"by":[79],"decrease":[81],"microenvironmental":[84],"pH.":[85],"These":[86],"features":[87],"are":[88],"also":[89],"maintained":[90],"purified":[93],"sample":[94],"vitro":[96],"both":[97,142],"presence":[100],"molecular":[103],"crowding":[104],"agent":[105],"mimicking":[106],"bacterial":[108],"environment,":[110],"biomimetic":[114],"extrusion":[115],"leading":[117],"fiber":[119],"formation.":[120],"Overall,":[121],"demonstrate":[123],"characterization":[125],"inside":[129],"E.":[130],"coli":[131],"could":[132],"be":[133],"used":[134],"basis":[137],"for":[138,141],"selecting":[139],"applications":[144],"fundamental":[147],"structure-function":[148],"studies.":[149]},"counts_by_year":[{"year":2026,"cited_by_count":6},{"year":2025,"cited_by_count":4},{"year":2024,"cited_by_count":7},{"year":2023,"cited_by_count":2}],"updated_date":"2026-11-12T12:28:57.059296","created_date":"2022-11-20T00:00:00"}