{"id":"https://openalex.org/W4308766205","doi":"https://doi.org/10.3390/bioengineering9110670","title":"Functional Expression of the Recombinant Spike Receptor Binding Domain of SARS-CoV-2 Omicron in the Periplasm of Escherichia coli","display_name":"Functional Expression of the Recombinant Spike Receptor Binding Domain of SARS-CoV-2 Omicron in the Periplasm of Escherichia coli","publication_year":2022,"publication_date":"2022-11-10","ids":{"openalex":"https://openalex.org/W4308766205","doi":"https://doi.org/10.3390/bioengineering9110670","pmid":"https://pubmed.ncbi.nlm.nih.gov/36354581","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/9687980"},"language":"en","primary_location":{"id":"doi:10.3390/bioengineering9110670","doi":"https://doi.org/10.3390/bioengineering9110670","is_oa":true,"landing_page_url":"https://doi.org/10.3390/bioengineering9110670","pdf_url":"https://www.mdpi.com/2306-5354/9/11/670/pdf?version=1668127590","source":{"id":"https://openalex.org/S4210179121","display_name":"Bioengineering","issn_l":"2306-5354","issn":["2306-5354"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","norway-1","poland-20","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioengineering","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.mdpi.com/2306-5354/9/11/670/pdf?version=1668127590","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101633808","display_name":"Woo Sung Kim","orcid":"https://orcid.org/0000-0002-4396-4403","observed_orcids":["https://orcid.org/0000-0002-4396-4403"]},"institutions":[{"id":"https://openalex.org/I9507","display_name":"Sangji University","ror":"https://ror.org/01gqe3t73","country_code":"KR","type":"education","lineage":["https://openalex.org/I9507"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Woo Sung Kim","raw_affiliation_strings":["Department of Pharmaceutical Engineering, Sangji University, Wonju 26339, Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmaceutical Engineering, Sangji University, Wonju 26339, Korea","institution_ids":["https://openalex.org/I9507"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100443862","display_name":"Ji Hyun Kim","orcid":"https://orcid.org/0000-0003-0861-2792","observed_orcids":["https://orcid.org/0000-0003-0861-2792","https://orcid.org/0000-0001-8477-0157","https://orcid.org/0000-0002-4746-5572"]},"institutions":[{"id":"https://openalex.org/I9507","display_name":"Sangji University","ror":"https://ror.org/01gqe3t73","country_code":"KR","type":"education","lineage":["https://openalex.org/I9507"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Ji Hyun Kim","raw_affiliation_strings":["Department of Pharmaceutical Engineering, Sangji University, Wonju 26339, Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmaceutical Engineering, Sangji University, Wonju 26339, Korea","institution_ids":["https://openalex.org/I9507"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100762467","display_name":"Jisun Lee","orcid":"https://orcid.org/0000-0003-1750-0063","observed_orcids":["https://orcid.org/0000-0003-1750-0063","https://orcid.org/0009-0001-5226-4900"]},"institutions":[{"id":"https://openalex.org/I197347611","display_name":"Korea University","ror":"https://ror.org/047dqcg40","country_code":"KR","type":"education","lineage":["https://openalex.org/I197347611"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Jisun Lee","raw_affiliation_strings":["BK21 Graduate Program, Department of Biomedical Sciences, Korea University College of Medicine, Seoul 02841, Korea","Department of Biomedical Sciences, Graduate School, Korea University, Seoul 02841, Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"BK21 Graduate Program, Department of Biomedical Sciences, Korea University College of Medicine, Seoul 02841, Korea","institution_ids":["https://openalex.org/I197347611"]},{"raw_affiliation_string":"Department of Biomedical Sciences, Graduate School, Korea University, Seoul 02841, Korea","institution_ids":["https://openalex.org/I197347611"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5079518270","display_name":"Su Yeon Ka","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I9507","display_name":"Sangji University","ror":"https://ror.org/01gqe3t73","country_code":"KR","type":"education","lineage":["https://openalex.org/I9507"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Su Yeon Ka","raw_affiliation_strings":["Department of Pharmaceutical Engineering, Sangji University, Wonju 26339, Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmaceutical Engineering, Sangji University, Wonju 26339, Korea","institution_ids":["https://openalex.org/I9507"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5028684096","display_name":"Hee Do Chae","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I9507","display_name":"Sangji University","ror":"https://ror.org/01gqe3t73","country_code":"KR","type":"education","lineage":["https://openalex.org/I9507"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Hee Do Chae","raw_affiliation_strings":["Department of Pharmaceutical Engineering, Sangji University, Wonju 26339, Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmaceutical Engineering, Sangji University, Wonju 26339, Korea","institution_ids":["https://openalex.org/I9507"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5034213644","display_name":"Inji Jung","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I197347611","display_name":"Korea University","ror":"https://ror.org/047dqcg40","country_code":"KR","type":"education","lineage":["https://openalex.org/I197347611"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Inji Jung","raw_affiliation_strings":["BK21 Graduate Program, Department of Biomedical Sciences, Korea University College of Medicine, Seoul 02841, Korea","Department of Biomedical Sciences, Graduate School, Korea University, Seoul 02841, Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"BK21 Graduate Program, Department of Biomedical Sciences, Korea University College of Medicine, Seoul 02841, Korea","institution_ids":["https://openalex.org/I197347611"]},{"raw_affiliation_string":"Department of Biomedical Sciences, Graduate School, Korea University, Seoul 02841, Korea","institution_ids":["https://openalex.org/I197347611"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5044505570","display_name":"Sang Taek Jung","orcid":"https://orcid.org/0000-0001-7217-979X","observed_orcids":["https://orcid.org/0000-0001-7217-979X"]},"institutions":[{"id":"https://openalex.org/I197347611","display_name":"Korea University","ror":"https://ror.org/047dqcg40","country_code":"KR","type":"education","lineage":["https://openalex.org/I197347611"]}],"countries":["KR"],"is_corresponding":true,"raw_author_name":"Sang Taek Jung","raw_affiliation_strings":["BK21 Graduate Program, Department of Biomedical Sciences, Korea University College of Medicine, Seoul 02841, Korea","Department of Biomedical Sciences, Graduate School, Korea University, Seoul 02841, Korea","Institute of Human Genetics, Korea University College of Medicine, Seoul 02841, Korea"],"raw_orcid":"https://orcid.org/0000-0001-7217-979X","affiliations":[{"raw_affiliation_string":"BK21 Graduate Program, Department of Biomedical Sciences, Korea University College of Medicine, Seoul 02841, Korea","institution_ids":["https://openalex.org/I197347611"]},{"raw_affiliation_string":"Department of Biomedical Sciences, Graduate School, Korea University, Seoul 02841, Korea","institution_ids":["https://openalex.org/I197347611"]},{"raw_affiliation_string":"Institute of Human Genetics, Korea University College of Medicine, Seoul 02841, Korea","institution_ids":["https://openalex.org/I197347611"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5058110250","display_name":"Jung\u2010Hyun Na","orcid":"https://orcid.org/0000-0002-5257-2428","observed_orcids":["https://orcid.org/0000-0002-5257-2428"]},"institutions":[{"id":"https://openalex.org/I9507","display_name":"Sangji University","ror":"https://ror.org/01gqe3t73","country_code":"KR","type":"education","lineage":["https://openalex.org/I9507"]}],"countries":["KR"],"is_corresponding":true,"raw_author_name":"Jung-Hyun Na","raw_affiliation_strings":["Department of Pharmaceutical Engineering, Sangji University, Wonju 26339, Korea","Research Institute of Korean Medicine, Sangji University, Wonju 26339, Korea"],"raw_orcid":"https://orcid.org/0000-0002-5257-2428","affiliations":[{"raw_affiliation_string":"Department of Pharmaceutical Engineering, Sangji University, Wonju 26339, Korea","institution_ids":["https://openalex.org/I9507"]},{"raw_affiliation_string":"Research Institute of Korean Medicine, Sangji University, Wonju 26339, Korea","institution_ids":["https://openalex.org/I9507"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5044505570","https://openalex.org/A5058110250"],"corresponding_institution_ids":["https://openalex.org/I197347611","https://openalex.org/I9507"],"apc_list":{"value":1700,"currency":"CHF","value_usd":1780},"apc_paid":{"value":1700,"currency":"CHF","value_usd":1780},"fwci":1.1357,"has_fulltext":true,"cited_by_count":9,"citation_normalized_percentile":{"value":0.77006603,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"9","issue":"11","first_page":"670","last_page":"670"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10118","display_name":"SARS-CoV-2 and COVID-19 Research","score":0.9897000193595886,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10118","display_name":"SARS-CoV-2 and COVID-19 Research","score":0.9897000193595886,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.003000000026077032,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.0026000000070780516,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/omicron-variant","display_name":"Omicron variant","score":0.9710000157356262},{"id":"https://openalex.org/keywords/spike-receptor-binding-domain","display_name":"spike protein receptor-binding domain","score":0.9800000190734863},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.9179999828338623},{"id":"https://openalex.org/keywords/escherichia-coli-periplasm","display_name":"Escherichia coli periplasm","score":0.9010000228881836},{"id":"https://openalex.org/keywords/disulfide-bond-formation","display_name":"disulfide bond formation","score":0.8759999871253967},{"id":"https://openalex.org/keywords/protein-disulfide-isomerase","display_name":"protein disulfide isomerase","score":0.9639999866485596},{"id":"https://openalex.org/keywords/protein-folding","display_name":"protein folding","score":0.765999972820282},{"id":"https://openalex.org/keywords/sotrovimab","display_name":"sotrovimab","score":0.8579999804496765},{"id":"https://openalex.org/keywords/cr3022","display_name":"CR3022","score":0.8899999856948853},{"id":"https://openalex.org/keywords/neutralizing-antibodies","display_name":"neutralizing antibodies","score":0.8849999904632568},{"id":"https://openalex.org/keywords/protein-subunit-vaccines","display_name":"subunit vaccine","score":0.9419999718666077}],"concepts":[{"id":"https://openalex.org/C201663137","wikidata":"https://www.wikidata.org/wiki/Q21198990","display_name":"Periplasmic space","level":4,"score":0.9291540384292603},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.7464903593063354},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.6571519374847412},{"id":"https://openalex.org/C2777648638","wikidata":"https://www.wikidata.org/wiki/Q57751738","display_name":"Coronavirus","level":5,"score":0.4882950782775879},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.47252729535102844},{"id":"https://openalex.org/C2775951584","wikidata":"https://www.wikidata.org/wiki/Q5309954","display_name":"DsbA","level":5,"score":0.47104716300964355},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4460964500904083},{"id":"https://openalex.org/C2775962898","wikidata":"https://www.wikidata.org/wiki/Q5073152","display_name":"Chaperone (clinical)","level":2,"score":0.44034823775291443},{"id":"https://openalex.org/C3007834351","wikidata":"https://www.wikidata.org/wiki/Q82069695","display_name":"Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)","level":5,"score":0.410919725894928},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.4087752103805542},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.36113131046295166},{"id":"https://openalex.org/C3008058167","wikidata":"https://www.wikidata.org/wiki/Q84263196","display_name":"Coronavirus disease 2019 (COVID-19)","level":4,"score":0.2832351326942444},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.22915664315223694},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.12476751208305359},{"id":"https://openalex.org/C524204448","wikidata":"https://www.wikidata.org/wiki/Q788926","display_name":"Infectious disease (medical specialty)","level":3,"score":0.08280974626541138},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.06785637140274048},{"id":"https://openalex.org/C2779134260","wikidata":"https://www.wikidata.org/wiki/Q12136","display_name":"Disease","level":2,"score":0.0},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.0}],"mesh":[],"locations_count":4,"locations":[{"id":"doi:10.3390/bioengineering9110670","doi":"https://doi.org/10.3390/bioengineering9110670","is_oa":true,"landing_page_url":"https://doi.org/10.3390/bioengineering9110670","pdf_url":"https://www.mdpi.com/2306-5354/9/11/670/pdf?version=1668127590","source":{"id":"https://openalex.org/S4210179121","display_name":"Bioengineering","issn_l":"2306-5354","issn":["2306-5354"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","norway-1","poland-20","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioengineering","raw_type":"journal-article"},{"id":"pmid:36354581","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/36354581","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioengineering (Basel, Switzerland)","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:9687980","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC9687980/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioengineering (Basel)","raw_type":"Text"},{"id":"pmh:oai:doaj.org/article:88a0f9be57b3471e9bf7de3ecf16e9e8","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/88a0f9be57b3471e9bf7de3ecf16e9e8","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Bioengineering, Vol 9, Iss 11, p 670 (2022)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.3390/bioengineering9110670","doi":"https://doi.org/10.3390/bioengineering9110670","is_oa":true,"landing_page_url":"https://doi.org/10.3390/bioengineering9110670","pdf_url":"https://www.mdpi.com/2306-5354/9/11/670/pdf?version=1668127590","source":{"id":"https://openalex.org/S4210179121","display_name":"Bioengineering","issn_l":"2306-5354","issn":["2306-5354"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","norway-1","poland-20","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioengineering","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.46810001134872437,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1219010277","display_name":null,"funder_award_id":"2021M3H9A1097596","funder_id":"https://openalex.org/F4320328359","funder_display_name":"Ministry of Science and ICT, South Korea"},{"id":"https://openalex.org/G1477921074","display_name":null,"funder_award_id":"2022R1A4A2000827","funder_id":"https://openalex.org/F4320328359","funder_display_name":"Ministry of Science and ICT, South Korea"},{"id":"https://openalex.org/G2292161915","display_name":null,"funder_award_id":"NRF-2021R1F1A1060240","funder_id":"https://openalex.org/F4320322120","funder_display_name":"National Research Foundation of Korea"},{"id":"https://openalex.org/G2830403459","display_name":null,"funder_award_id":"2020M3E5E2037775","funder_id":"https://openalex.org/F4320328359","funder_display_name":"Ministry of Science and ICT, South Korea"},{"id":"https://openalex.org/G4968345064","display_name":null,"funder_award_id":"2021R1F1A1060240","funder_id":"https://openalex.org/F4320328359","funder_display_name":"Ministry of Science and ICT, South Korea"},{"id":"https://openalex.org/G6649665881","display_name":null,"funder_award_id":"2021M3H9A1097596","funder_id":"https://openalex.org/F4320322120","funder_display_name":"National Research Foundation of Korea"}],"funders":[{"id":"https://openalex.org/F4320320671","display_name":"National Research Foundation","ror":"https://ror.org/05s0g1g46"},{"id":"https://openalex.org/F4320322120","display_name":"National Research Foundation of Korea","ror":"https://ror.org/013aysd81"},{"id":"https://openalex.org/F4320328359","display_name":"Ministry of Science and ICT, South Korea","ror":"https://ror.org/01wpjm123"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4308766205.pdf","grobid_xml":"https://content.openalex.org/works/W4308766205.grobid-xml"},"referenced_works_count":36,"referenced_works":["https://openalex.org/W1950786799","https://openalex.org/W1973718144","https://openalex.org/W2085206766","https://openalex.org/W2105103454","https://openalex.org/W2136364313","https://openalex.org/W2266449668","https://openalex.org/W2401115505","https://openalex.org/W2769227721","https://openalex.org/W2800284038","https://openalex.org/W3014067025","https://openalex.org/W3015835940","https://openalex.org/W3022261482","https://openalex.org/W3092975157","https://openalex.org/W3133516577","https://openalex.org/W3154076884","https://openalex.org/W3157625523","https://openalex.org/W3174130818","https://openalex.org/W3176526573","https://openalex.org/W3192172700","https://openalex.org/W3201571176","https://openalex.org/W3214307669","https://openalex.org/W3214900277","https://openalex.org/W4205183254","https://openalex.org/W4213222685","https://openalex.org/W4214573802","https://openalex.org/W4214729818","https://openalex.org/W4214863616","https://openalex.org/W4220807057","https://openalex.org/W4220886953","https://openalex.org/W4221109130","https://openalex.org/W4226087845","https://openalex.org/W4229022963","https://openalex.org/W4283010973","https://openalex.org/W4283831060","https://openalex.org/W4286009139","https://openalex.org/W4295760568"],"related_works":["https://openalex.org/W4412522326","https://openalex.org/W4386903256","https://openalex.org/W3174130818","https://openalex.org/W7167926530","https://openalex.org/W4313244079","https://openalex.org/W7117958849","https://openalex.org/W3160631959","https://openalex.org/W4414909150","https://openalex.org/W4210397113","https://openalex.org/W3126640059"],"abstract_inverted_index":{"A":[0],"new":[1],"severe":[2],"acute":[3],"respiratory":[4],"syndrome":[5],"coronavirus":[6,23],"2":[7,147],"(SARS-CoV-2)":[8],"variant":[9],"known":[10],"as":[11],"Omicron":[12,32,88,103,138,155],"has":[13,57],"caused":[14],"a":[15,36,47,51,71,75,93],"rapid":[16],"increase":[17],"in":[18,60,70,99,158],"recent":[19],"global":[20],"patients":[21],"with":[22],"infectious":[24],"disease":[25],"2019":[26],"(COVID-19).":[27],"To":[28],"overcome":[29],"the":[30,61,66,86,132,136,143,154,159,184],"COVID-19":[31],"variant,":[33],"production":[34,62],"of":[35,63,135,153,183],"recombinant":[37,64],"spike":[38,67,181],"receptor":[39],"binding":[40,141],"domain":[41],"(RBD)":[42],"is":[43],"vital":[44],"for":[45],"developing":[46],"subunit":[48],"vaccine":[49],"or":[50],"neutralizing":[52],"antibody.":[53],"Although":[54],"bacterial":[55,72],"expression":[56,97,161,170],"many":[58],"advantages":[59],"proteins,":[65],"RBD":[68,89,104,139,156],"expressed":[69],"system":[73,98,171],"experiences":[74],"folding":[76],"problem":[77],"related":[78],"to":[79,124,126,142,151,175],"disulfide":[80,94],"bond":[81],"formation.":[82],"In":[83,130],"this":[84],"study,":[85],"soluble":[87],"was":[90,109],"obtained":[91],"by":[92,113],"isomerase-assisted":[95],"periplasmic":[96],"Escherichia":[100],"coli.":[101],"The":[102],"purified":[105,137],"from":[106],"E.":[107,168],"coli":[108,169],"very":[110],"well":[111],"recognized":[112],"anti-SARS-CoV-2":[114],"antibodies,":[115],"sotrovimab":[116],"(S309),":[117],"and":[118,178,190],"CR3022,":[119],"which":[120],"were":[121,149],"previously":[122],"reported":[123],"bind":[125],"various":[127],"SARS-CoV-2":[128,187],"variants.":[129],"addition,":[131],"kinetic":[133],"parameters":[134],"upon":[140],"human":[144],"angiotensin-converting":[145],"enzyme":[146],"(ACE2)":[148],"similar":[150],"those":[152],"produced":[157],"mammalian":[160],"system.":[162],"These":[163],"results":[164],"suggest":[165],"that":[166],"an":[167],"would":[172],"be":[173],"suitable":[174],"produce":[176],"functional":[177],"correctly":[179],"folded":[180],"RBDs":[182],"next":[185],"emerging":[186],"variants":[188],"quickly":[189],"inexpensively.":[191]},"counts_by_year":[{"year":2025,"cited_by_count":4},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2022-11-15T00:00:00"}