{"id":"https://openalex.org/W4308398794","doi":"https://doi.org/10.1136/jitc-2022-sitc2022.0352","title":"352 STASH select, a simple and robust method of purifying cells engineered with multiple vectors using a single selection marker","display_name":"352 STASH select, a simple and robust method of purifying cells engineered with multiple vectors using a single selection marker","publication_year":2022,"publication_date":"2022-11-01","ids":{"openalex":"https://openalex.org/W4308398794","doi":"https://doi.org/10.1136/jitc-2022-sitc2022.0352"},"language":"en","primary_location":{"id":"doi:10.1136/jitc-2022-sitc2022.0352","doi":"https://doi.org/10.1136/jitc-2022-sitc2022.0352","is_oa":true,"landing_page_url":"https://doi.org/10.1136/jitc-2022-sitc2022.0352","pdf_url":"https://jitc.bmj.com/content/jitc/10/Suppl_2/A371.full.pdf","source":{"id":"https://openalex.org/S2754803477","display_name":"Journal for ImmunoTherapy of Cancer","issn_l":"2051-1426","issn":["2051-1426"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","oarl","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319945","host_organization_name":"BMJ","host_organization_lineage":["https://openalex.org/P4310319945"],"host_organization_lineage_names":["BMJ"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Regular and Young Investigator Award Abstracts","raw_type":"proceedings-article"},"volume":null,"type":"conference-abstract","indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://jitc.bmj.com/content/jitc/10/Suppl_2/A371.full.pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5080476711","display_name":"Louai Labanieh","orcid":"https://orcid.org/0000-0003-3998-7415","observed_orcids":["https://orcid.org/0000-0003-3998-7415"]},"institutions":[{"id":"https://openalex.org/I97018004","display_name":"Stanford University","ror":"https://ror.org/00f54p054","country_code":"US","type":"education","lineage":["https://openalex.org/I97018004"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Louai Labanieh","raw_affiliation_strings":["Stanford University, Stanford, CA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Stanford University, Stanford, CA, USA","institution_ids":["https://openalex.org/I97018004"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5035732385","display_name":"Kaithlen Zen Pacheco","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I97018004","display_name":"Stanford University","ror":"https://ror.org/00f54p054","country_code":"US","type":"education","lineage":["https://openalex.org/I97018004"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kaithlen Zen Pacheco","raw_affiliation_strings":["Stanford University, Stanford, CA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Stanford University, Stanford, CA, USA","institution_ids":["https://openalex.org/I97018004"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5033972749","display_name":"Robbie G. Majzner","orcid":"https://orcid.org/0000-0001-6969-8011","observed_orcids":["https://orcid.org/0000-0001-6969-8011"]},"institutions":[{"id":"https://openalex.org/I97018004","display_name":"Stanford University","ror":"https://ror.org/00f54p054","country_code":"US","type":"education","lineage":["https://openalex.org/I97018004"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Robbie Majzner","raw_affiliation_strings":["Stanford University, Stanford, CA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Stanford University, Stanford, CA, USA","institution_ids":["https://openalex.org/I97018004"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103044637","display_name":"Chris Fisher","orcid":"https://orcid.org/0000-0001-9916-5291","observed_orcids":["https://orcid.org/0000-0001-9916-5291"]},"institutions":[{"id":"https://openalex.org/I97018004","display_name":"Stanford University","ror":"https://ror.org/00f54p054","country_code":"US","type":"education","lineage":["https://openalex.org/I97018004"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Chris Fisher","raw_affiliation_strings":["Stanford University, Stanford, CA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Stanford University, Stanford, CA, USA","institution_ids":["https://openalex.org/I97018004"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5065321275","display_name":"Elena Sotillo","orcid":"https://orcid.org/0000-0002-3993-1932","observed_orcids":["https://orcid.org/0000-0002-3993-1932"]},"institutions":[{"id":"https://openalex.org/I97018004","display_name":"Stanford University","ror":"https://ror.org/00f54p054","country_code":"US","type":"education","lineage":["https://openalex.org/I97018004"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Elena Sotillo","raw_affiliation_strings":["Stanford University, Stanford, CA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Stanford University, Stanford, CA, USA","institution_ids":["https://openalex.org/I97018004"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100396263","display_name":"Peng Xu","orcid":"https://orcid.org/0000-0002-7573-4813","observed_orcids":["https://orcid.org/0000-0002-7573-4813"]},"institutions":[{"id":"https://openalex.org/I97018004","display_name":"Stanford University","ror":"https://ror.org/00f54p054","country_code":"US","type":"education","lineage":["https://openalex.org/I97018004"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Peng Xu","raw_affiliation_strings":["Stanford University, Stanford, CA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Stanford University, Stanford, CA, USA","institution_ids":["https://openalex.org/I97018004"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5058204963","display_name":"Jennifer R. Cochran","orcid":"https://orcid.org/0000-0002-6532-9873","observed_orcids":["https://orcid.org/0000-0002-6532-9873"]},"institutions":[{"id":"https://openalex.org/I97018004","display_name":"Stanford University","ror":"https://ror.org/00f54p054","country_code":"US","type":"education","lineage":["https://openalex.org/I97018004"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jennifer Cochran","raw_affiliation_strings":["Stanford University, Stanford, CA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Stanford University, Stanford, CA, USA","institution_ids":["https://openalex.org/I97018004"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5087779758","display_name":"Crystal Lee Mackall","orcid":"https://orcid.org/0000-0003-0359-9023","observed_orcids":["https://orcid.org/0000-0003-0359-9023","https://orcid.org/0000-0001-6323-4304"]},"institutions":[{"id":"https://openalex.org/I97018004","display_name":"Stanford University","ror":"https://ror.org/00f54p054","country_code":"US","type":"education","lineage":["https://openalex.org/I97018004"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Crystal Mackall","raw_affiliation_strings":["Stanford University, Stanford, CA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Stanford University, Stanford, CA, USA","institution_ids":["https://openalex.org/I97018004"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I97018004"],"apc_list":{"value":2490,"currency":"USD","value_usd":2490},"apc_paid":{"value":2490,"currency":"USD","value_usd":2490},"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":"A371","last_page":"A371"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11491","display_name":"CAR-T cell therapy research","score":0.9916999936103821,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11491","display_name":"CAR-T cell therapy research","score":0.9916999936103821,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.004900000058114529,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.0007999999797903001,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/car-t-cell-engineering","display_name":"CAR-T cell engineering","score":0.7110000252723694},{"id":"https://openalex.org/keywords/tev-protease","display_name":"TEV protease","score":0.8830000162124634},{"id":"https://openalex.org/keywords/t-cell-exhaustion","display_name":"T cell exhaustion","score":0.5210000276565552},{"id":"https://openalex.org/keywords/t-cell-persistence","display_name":"T cell persistence","score":0.5230000019073486},{"id":"https://openalex.org/keywords/antigen-loss","display_name":"antigen loss","score":0.5899999737739563},{"id":"https://openalex.org/keywords/tumor-heterogeneity","display_name":"tumor heterogeneity","score":0.7440000176429749}],"concepts":[{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.49025586247444153},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.47947725653648376},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.4396423399448395},{"id":"https://openalex.org/C553184892","wikidata":"https://www.wikidata.org/wiki/Q1141429","display_name":"Flow cytometry","level":2,"score":0.4188433885574341},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.33963489532470703},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.18313324451446533},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.13312745094299316}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1136/jitc-2022-sitc2022.0352","doi":"https://doi.org/10.1136/jitc-2022-sitc2022.0352","is_oa":true,"landing_page_url":"https://doi.org/10.1136/jitc-2022-sitc2022.0352","pdf_url":"https://jitc.bmj.com/content/jitc/10/Suppl_2/A371.full.pdf","source":{"id":"https://openalex.org/S2754803477","display_name":"Journal for ImmunoTherapy of Cancer","issn_l":"2051-1426","issn":["2051-1426"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","oarl","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319945","host_organization_name":"BMJ","host_organization_lineage":["https://openalex.org/P4310319945"],"host_organization_lineage_names":["BMJ"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Regular and Young Investigator Award Abstracts","raw_type":"proceedings-article"}],"best_oa_location":{"id":"doi:10.1136/jitc-2022-sitc2022.0352","doi":"https://doi.org/10.1136/jitc-2022-sitc2022.0352","is_oa":true,"landing_page_url":"https://doi.org/10.1136/jitc-2022-sitc2022.0352","pdf_url":"https://jitc.bmj.com/content/jitc/10/Suppl_2/A371.full.pdf","source":{"id":"https://openalex.org/S2754803477","display_name":"Journal for ImmunoTherapy of Cancer","issn_l":"2051-1426","issn":["2051-1426"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","oarl","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319945","host_organization_name":"BMJ","host_organization_lineage":["https://openalex.org/P4310319945"],"host_organization_lineage_names":["BMJ"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Regular and Young Investigator Award Abstracts","raw_type":"proceedings-article"},"sustainable_development_goals":[{"score":0.9391000270843506,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4308398794.pdf","grobid_xml":"https://content.openalex.org/works/W4308398794.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4405044356","https://openalex.org/W3179118015","https://openalex.org/W4415530894","https://openalex.org/W4386744221","https://openalex.org/W4385892009","https://openalex.org/W3096012644","https://openalex.org/W3029543443","https://openalex.org/W2746186641","https://openalex.org/W4405700055","https://openalex.org/W4401204138"],"abstract_inverted_index":{"Background":[0],"CAR-T":[1,149],"cell":[2,22,110,234],"therapies":[3,68,395],"have":[4],"demonstrated":[5],"remarkable":[6],"clinical":[7,125],"benefit":[8],"in":[9,62,107,123,214,306],"liquid":[10],"tumors,":[11,47],"but":[12],"significant":[13],"obstacles":[14],"such":[15],"as":[16,272],"antigen":[17],"loss,":[18],"tumor":[19,64,162],"heterogeneity,":[20],"T":[21,48,309],"exhaustion,":[23,57],"and":[24,30,39,58,156,167,190,227,243,286,311,344],"poor":[25],"persistence":[26],"limit":[27],"the":[28,63,75,87,95,124,176,194,197,205,209,217,222,225,233,237,244,303,341,345,401],"frequency":[29],"durability":[31],"of":[32,43,77,90,97,173,180,196,208,216,265,320,362,376],"complete":[33],"responses.":[34],"To":[35],"overcome":[36],"these":[37,70],"challenges":[38],"expand":[40],"this":[41,330],"class":[42],"therapeutics":[44],"to":[45,52,56,230,232,314,322,332,380,391],"solid":[46],"cells":[49,102,137,150,257,310,377],"will":[50,73],"need":[51],"be":[53,270],"multi-specific,":[54],"resistant":[55],"maintain":[59],"their":[60],"fitness":[61],"microenvironment.":[65],"Engineering":[66],"NextGen":[67,393],"with":[69,103,259,367],"enhanced":[71],"capabilities":[72],"require":[74],"introduction":[76],"additional":[78],"genetic":[79,140],"modules":[80,399],"that":[81,112,268,295],"often":[82],"do":[83],"not":[84,120],"fit":[85],"within":[86],"payload":[88],"capacity":[89],"a":[91,108,132,143,181,186,247,273,333,354,368,386],"single":[92,144],"vector,":[93,117],"necessitating":[94],"use":[96],"multiple":[98,104,139,397],"vectors.":[99],"However,":[100],"engineering":[101],"vectors":[105,261,352],"results":[106,213],"heterogeneous":[109],"product":[111],"requires":[113],"purification":[114,361],"for":[115,135,276,360],"each":[116],"which":[118,201,220,358],"is":[119,175,385],"currently":[121],"possible":[122],"setting.":[126],"Here,":[127],"we":[128],"present":[129],"STASH":[130],"Select,":[131],"simple":[133],"method":[134],"enriching":[136,372],"containing":[138,396],"modifications":[141],"using":[142,336],"selection":[145,199,274,388],"marker.":[146,183],"Methods":[147],"Human":[148],"were":[151,312],"generated":[152],"from":[153,224,378],"healthy":[154],"donors":[155],"characterized":[157],"by":[158],"flow":[159],"cytometry,":[160],"ELISA,":[161],"killing":[163],"assays,":[164],"bulk":[165],"RNAseq,":[166],"MACS.":[168,327],"Results":[169],"The":[170],"core":[171],"principle":[172],"STASH-Select":[174,299,335,384],"conditional":[177],"cellular":[178],"localization":[179],"selectable":[182],"We":[184,280,301,328],"integrate":[185],"protease":[187,212,245],"cleavage":[188,215],"site":[189],"an":[191,251,316,373],"ER-Tag":[192],"onto":[193,349],"C-terminus":[195],"EGFRt":[198,223,239,267,326,343],"marker,":[200],"\u201cSTASHes\u201d":[202],"it":[203,229],"inside":[204],"cell.":[206],"Co-expression":[207],"cognate":[210],"TEV":[211,338,347],"ER":[218,226,283,293],"Tag,":[219],"releases":[221],"allows":[228],"translocate":[231],"surface.":[235],"Encoding":[236],"STASHed":[238,342],"on":[240,246],"one":[241],"vector":[242,249],"second":[248],"creates":[250],"AND":[252,356],"gate":[253,357],"system,":[254],"where":[255],"only":[256],"transduced":[258],"both":[260],"express":[262],"high":[263,297],"levels":[264],"surface":[266],"can":[269],"used":[271],"handle":[275],"isolating":[277],"double+":[278,318],"cells.":[279],"screened":[281],"22":[282],"retention":[284],"domains":[285],"identified":[287],"four":[288],"novel,":[289],"compact":[290],"(<45aa)":[291],"human":[292,308],"tags":[294],"yield":[296],"performance":[298],"systems.":[300],"tested":[302],"two-vector":[304],"STASH-select":[305],"primary":[307],"able":[313],"enrich":[315],"initial":[317,374],"population":[319,375],"12.3%":[321],"96.3%":[323],"purity":[324],"via":[325],"expanded":[329],"approach":[331],"three-vector":[334],"Split":[337],"protease.":[339],"Configuring":[340],"two":[346],"fragments":[348],"three":[350,363],"separate":[351],"produces":[353],"3-vector":[355],"allowed":[359],"clinically":[364],"relevant":[365],"cargos":[366],"single-step":[369],"MACS":[370],"selection,":[371],"16.7%":[379],">90%":[381],"triple+.":[382],"Conclusions":[383],"novel":[387],"platform":[389],"designed":[390],"propel":[392],"smart":[394],"enhancing":[398],"into":[400],"clinic.":[402]},"counts_by_year":[],"updated_date":"2026-11-02T09:45:03.842371","created_date":"2022-11-11T00:00:00"}