{"id":"https://openalex.org/W4302007897","doi":"https://doi.org/10.1038/s41598-022-20993-z","title":"Cryo-derived plants through embryogenesis showed same levels of vinblastine and vincristine (anticancer) in Catharanthus roseus and had normal genome size","display_name":"Cryo-derived plants through embryogenesis showed same levels of vinblastine and vincristine (anticancer) in Catharanthus roseus and had normal genome size","publication_year":2022,"publication_date":"2022-10-05","ids":{"openalex":"https://openalex.org/W4302007897","doi":"https://doi.org/10.1038/s41598-022-20993-z","pmid":"https://pubmed.ncbi.nlm.nih.gov/36198853","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/9534890"},"language":"en","primary_location":{"id":"doi:10.1038/s41598-022-20993-z","doi":"https://doi.org/10.1038/s41598-022-20993-z","is_oa":true,"landing_page_url":"https://doi.org/10.1038/s41598-022-20993-z","pdf_url":"https://www.nature.com/articles/s41598-022-20993-z.pdf","source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.nature.com/articles/s41598-022-20993-z.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5057876904","display_name":"A. Mujib","orcid":"https://orcid.org/0000-0002-4104-6887","observed_orcids":["https://orcid.org/0000-0002-4104-6887"]},"institutions":[{"id":"https://openalex.org/I19716509","display_name":"Jamia Hamdard","ror":"https://ror.org/03dwxvb85","country_code":"IN","type":"education","lineage":["https://openalex.org/I19716509"]}],"countries":["IN"],"is_corresponding":true,"raw_author_name":"A. Mujib","raw_affiliation_strings":["Cellular Differentiation and Molecular Genetics Section, Department of Botany, Jamia Hamdard, New Delhi, India. amujib3@yahoo.co.in","Cellular Differentiation and Molecular Genetics Section, Department of Botany, Jamia Hamdard, New Delhi, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cellular Differentiation and Molecular Genetics Section, Department of Botany, Jamia Hamdard, New Delhi, India. amujib3@yahoo.co.in","institution_ids":["https://openalex.org/I19716509"]},{"raw_affiliation_string":"Cellular Differentiation and Molecular Genetics Section, Department of Botany, Jamia Hamdard, New Delhi, India","institution_ids":["https://openalex.org/I19716509"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101089447","display_name":"Samar Fatima","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I19716509","display_name":"Jamia Hamdard","ror":"https://ror.org/03dwxvb85","country_code":"IN","type":"education","lineage":["https://openalex.org/I19716509"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Samar Fatima","raw_affiliation_strings":["Cellular Differentiation and Molecular Genetics Section, Department of Botany, Jamia Hamdard, New Delhi, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cellular Differentiation and Molecular Genetics Section, Department of Botany, Jamia Hamdard, New Delhi, India","institution_ids":["https://openalex.org/I19716509"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5031145441","display_name":"Moien Qadir Malik","orcid":"https://orcid.org/0000-0001-8907-0600","observed_orcids":["https://orcid.org/0000-0001-8907-0600"]},"institutions":[{"id":"https://openalex.org/I19716509","display_name":"Jamia Hamdard","ror":"https://ror.org/03dwxvb85","country_code":"IN","type":"education","lineage":["https://openalex.org/I19716509"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Moien Qadir Malik","raw_affiliation_strings":["Cellular Differentiation and Molecular Genetics Section, Department of Botany, Jamia Hamdard, New Delhi, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cellular Differentiation and Molecular Genetics Section, Department of Botany, Jamia Hamdard, New Delhi, India","institution_ids":["https://openalex.org/I19716509"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5057876904"],"corresponding_institution_ids":["https://openalex.org/I19716509"],"apc_list":{"value":2190,"currency":"USD","value_usd":2190},"apc_paid":{"value":2190,"currency":"USD","value_usd":2190},"fwci":0.9119,"has_fulltext":true,"cited_by_count":12,"citation_normalized_percentile":{"value":0.69100452,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":98},"biblio":{"volume":"12","issue":"1","first_page":"16635","last_page":"16635"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10014","display_name":"Plant Stress Responses and Tolerance","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10364","display_name":"Reproductive Biology and Fertility","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/catharanthus-roseus","display_name":"Catharanthus roseus","score":0.9869999885559082},{"id":"https://openalex.org/keywords/cryopreservation","display_name":"cryopreservation","score":0.9599999785423279},{"id":"https://openalex.org/keywords/embryogenic-callus","display_name":"embryogenic callus","score":0.7749999761581421},{"id":"https://openalex.org/keywords/tissue-bank","display_name":"tissue banking","score":0.753000020980835},{"id":"https://openalex.org/keywords/cryoprotectants","display_name":"cryoprotectants","score":0.7200000286102295},{"id":"https://openalex.org/keywords/polyethylene-glycol","display_name":"polyethylene glycol","score":0.8420000076293945},{"id":"https://openalex.org/keywords/sucrose","display_name":"sucrose","score":0.7279999852180481},{"id":"https://openalex.org/keywords/dehydration-food-drying-and-plant-water-stress","display_name":"dehydration (food drying and plant water stress)","score":0.6650000214576721},{"id":"https://openalex.org/keywords/somatic-embryogenesis","display_name":"somatic embryogenesis","score":0.8029999732971191},{"id":"https://openalex.org/keywords/plantlet-regeneration","display_name":"plantlet regeneration","score":0.8119999766349792},{"id":"https://openalex.org/keywords/genome-size","display_name":"genome size","score":0.6890000104904175},{"id":"https://openalex.org/keywords/2c-dna-content","display_name":"2C DNA content","score":0.8009999990463257},{"id":"https://openalex.org/keywords/vinblastine","display_name":"vinblastine","score":0.8820000290870667},{"id":"https://openalex.org/keywords/vincristine","display_name":"vincristine","score":0.9959999918937683},{"id":"https://openalex.org/keywords/alkaloid-production","display_name":"alkaloid production","score":0.7059999704360962},{"id":"https://openalex.org/keywords/germplasm-conservation","display_name":"germplasm conservation","score":0.6769999861717224},{"id":"https://openalex.org/keywords/naa","display_name":"NAA","score":0.3149999976158142}],"concepts":[{"id":"https://openalex.org/C2779906556","wikidata":"https://www.wikidata.org/wiki/Q161093","display_name":"Catharanthus roseus","level":2,"score":0.9356549978256226},{"id":"https://openalex.org/C179933525","wikidata":"https://www.wikidata.org/wiki/Q1144664","display_name":"Cryopreservation","level":3,"score":0.8240882754325867},{"id":"https://openalex.org/C2778597767","wikidata":"https://www.wikidata.org/wiki/Q4027534","display_name":"Sucrose","level":2,"score":0.7714179754257202},{"id":"https://openalex.org/C2776779347","wikidata":"https://www.wikidata.org/wiki/Q245280","display_name":"Sorbitol","level":2,"score":0.7566015720367432},{"id":"https://openalex.org/C201468095","wikidata":"https://www.wikidata.org/wiki/Q7559239","display_name":"Somatic embryogenesis","level":4,"score":0.7065271139144897},{"id":"https://openalex.org/C68528507","wikidata":"https://www.wikidata.org/wiki/Q692267","display_name":"Cryoprotectant","level":4,"score":0.6777954697608948},{"id":"https://openalex.org/C2780881558","wikidata":"https://www.wikidata.org/wiki/Q132501","display_name":"Glycerol","level":2,"score":0.6445863246917725},{"id":"https://openalex.org/C9549007","wikidata":"https://www.wikidata.org/wiki/Q1072909","display_name":"Callus","level":2,"score":0.5663439035415649},{"id":"https://openalex.org/C2778445172","wikidata":"https://www.wikidata.org/wiki/Q407646","display_name":"Mannitol","level":2,"score":0.4880160391330719},{"id":"https://openalex.org/C146763376","wikidata":"https://www.wikidata.org/wiki/Q81714","display_name":"Petri dish","level":2,"score":0.4763615131378174},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4730619192123413},{"id":"https://openalex.org/C53227056","wikidata":"https://www.wikidata.org/wiki/Q189152","display_name":"Viability assay","level":3,"score":0.4688827693462372},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.4641757011413574},{"id":"https://openalex.org/C200806820","wikidata":"https://www.wikidata.org/wiki/Q1798348","display_name":"Murashige and Skoog medium","level":4,"score":0.4546809494495392},{"id":"https://openalex.org/C2778452553","wikidata":"https://www.wikidata.org/wiki/Q407927","display_name":"Dimethyl sulfoxide","level":2,"score":0.44385749101638794},{"id":"https://openalex.org/C2777207623","wikidata":"https://www.wikidata.org/wiki/Q3391243","display_name":"Saccharum officinarum","level":2,"score":0.4431299567222595},{"id":"https://openalex.org/C144027150","wikidata":"https://www.wikidata.org/wiki/Q48803","display_name":"Horticulture","level":1,"score":0.40224742889404297},{"id":"https://openalex.org/C90703135","wikidata":"https://www.wikidata.org/wiki/Q3287622","display_name":"Tissue culture","level":3,"score":0.3894687592983246},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.35334211587905884},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3351321518421173},{"id":"https://openalex.org/C196843134","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryo","level":2,"score":0.2497280240058899},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.20152625441551208},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.16647467017173767},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.09070196747779846},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.08929020166397095},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D003451","descriptor_name":"Cryoprotective Agents","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D003681","descriptor_name":"Dehydration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004121","descriptor_name":"Dimethyl Sulfoxide","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D005990","descriptor_name":"Glycerol","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009584","descriptor_name":"Nitrogen","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011092","descriptor_name":"Polyethylene Glycols","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013012","descriptor_name":"Sorbitol","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D013395","descriptor_name":"Sucrose","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014747","descriptor_name":"Vinblastine","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D014750","descriptor_name":"Vincristine","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D014867","descriptor_name":"Water","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015925","descriptor_name":"Cryopreservation","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D030008","descriptor_name":"Catharanthus","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D047108","descriptor_name":"Embryonic Development","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D059646","descriptor_name":"Genome Size","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1038/s41598-022-20993-z","doi":"https://doi.org/10.1038/s41598-022-20993-z","is_oa":true,"landing_page_url":"https://doi.org/10.1038/s41598-022-20993-z","pdf_url":"https://www.nature.com/articles/s41598-022-20993-z.pdf","source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},{"id":"pmid:36198853","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/36198853","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific reports","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:9534890","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC9534890/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Sci Rep","raw_type":"Text"},{"id":"pmh:oai:doaj.org/article:78c4278f07704495b0b20326e584f2bf","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/78c4278f07704495b0b20326e584f2bf","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Scientific Reports, Vol 12, Iss 1, Pp 1-13 (2022)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1038/s41598-022-20993-z","doi":"https://doi.org/10.1038/s41598-022-20993-z","is_oa":true,"landing_page_url":"https://doi.org/10.1038/s41598-022-20993-z","pdf_url":"https://www.nature.com/articles/s41598-022-20993-z.pdf","source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.5683000087738037,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320324341","display_name":"Hamdard National Foundation","ror":"https://ror.org/038gm9q58"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4302007897.pdf","grobid_xml":"https://content.openalex.org/works/W4302007897.grobid-xml"},"referenced_works_count":50,"referenced_works":["https://openalex.org/W121317139","https://openalex.org/W134934419","https://openalex.org/W169242649","https://openalex.org/W257395024","https://openalex.org/W1503882977","https://openalex.org/W1822591004","https://openalex.org/W1973801449","https://openalex.org/W1983265991","https://openalex.org/W1984683682","https://openalex.org/W1984750101","https://openalex.org/W1984931665","https://openalex.org/W1993864250","https://openalex.org/W2004733136","https://openalex.org/W2007266246","https://openalex.org/W2008355460","https://openalex.org/W2012567519","https://openalex.org/W2018924041","https://openalex.org/W2024556021","https://openalex.org/W2031335976","https://openalex.org/W2051820203","https://openalex.org/W2051846901","https://openalex.org/W2054746091","https://openalex.org/W2063595449","https://openalex.org/W2066352067","https://openalex.org/W2076485862","https://openalex.org/W2083732477","https://openalex.org/W2090938271","https://openalex.org/W2094066425","https://openalex.org/W2115868389","https://openalex.org/W2121290582","https://openalex.org/W2122753176","https://openalex.org/W2143139062","https://openalex.org/W2149894743","https://openalex.org/W2151614210","https://openalex.org/W2164980084","https://openalex.org/W2409017673","https://openalex.org/W2556602392","https://openalex.org/W2741271627","https://openalex.org/W2775319552","https://openalex.org/W2790070876","https://openalex.org/W2891727924","https://openalex.org/W2897111944","https://openalex.org/W2916703707","https://openalex.org/W2919780667","https://openalex.org/W2981583043","https://openalex.org/W3007141522","https://openalex.org/W3040670966","https://openalex.org/W3095888772","https://openalex.org/W3105154117","https://openalex.org/W3217529580"],"related_works":["https://openalex.org/W2094066425","https://openalex.org/W2062920804","https://openalex.org/W2460544946","https://openalex.org/W2018849868","https://openalex.org/W2016512941","https://openalex.org/W3159842314","https://openalex.org/W4206604551","https://openalex.org/W1984750101","https://openalex.org/W2097416362","https://openalex.org/W66737191"],"abstract_inverted_index":{"Cryopreservation":[0],"of":[1,23,38,57,63,143,206,225,252,299,339],"rare":[2],"plant":[3,52],"materials":[4],"is":[5,13,27,48,306],"an":[6,183],"important":[7,51],"approach":[8],"for":[9,158,165,201,336,341],"preserving":[10],"germplasms":[11],"and":[12,41,60,87,108,110,120,135,173,181,204,236,244,274,287,313,323],"a":[14,49,55],"good":[15],"added":[16,278],"concept":[17],"to":[18,90,100,127,197,308],"tissue":[19,72,150,286],"banking.":[20],"The":[21,259,296,328],"preservation":[22],"embryogenic":[24,71,208],"cell":[25,66,186],"suspensions":[26],"even":[28],"more":[29,147],"valuable":[30],"as":[31],"the":[32,81,96,141,211,216,223,288,318],"tissues":[33,97],"facilitate":[34],"in":[35,77,152,250,270,320],"producing":[36,65],"millions":[37],"embryos,":[39],"plantlets":[40],"generates":[42],"transgenics":[43],"en":[44],"masse.":[45],"Catharanthus":[46,310],"roseus":[47,79],"medicinally":[50],"that":[53],"produces":[54],"variety":[56],"anticancerous":[58],"phytocompounds":[59],"needs":[61],"conservation":[62],"alkaloid":[64],"lines.":[67],"In":[68],"this":[69],"study,":[70],"banking":[73],"has":[74],"been":[75],"attempted":[76],"C.":[78],"by":[80,162],"two-step":[82],"cryopreservation":[83,330],"method":[84],"combining":[85],"cryoprotection":[86],"dehydration.":[88],"Prior":[89],"plunging":[91],"into":[92,263],"liquid":[93],"nitrogen":[94],"(LN),":[95],"were":[98,122,281,316],"exposed":[99],"osmotic-and":[101],"cryoprotective":[102,112,214],"agents.":[103],"Two":[104],"osmotic":[105],"agents":[106],"(sugar":[107],"sorbitol)":[109],"three":[111],"compounds,":[113],"polyethylene":[114],"glycol":[115],"(PEG),":[116],"dimethyl":[117],"sulfoxide":[118],"(DMSO)":[119],"glycerol":[121],"used":[123,335],"at":[124],"varying":[125],"concentrations":[126],"protect":[128],"cells":[129],"from":[130,284],"freezing":[131],"damages.":[132],"Both":[133],"sucrose":[134,144,157,230,255],"sorbitol":[136],"increased":[137],"callus":[138],"biomass":[139],"post-cryopreservation;":[140],"influence":[142],"was":[145,195,220,248,291,302],"however,":[146],"prominent.":[148],"Embryogenic":[149],"treated":[151],"medium":[153],"with":[154,227],"0.4":[155,228,253],"M":[156,229,254],"2":[159,166],"days":[160],"followed":[161],"5%":[163,257],"PEG":[164,233],"h":[167],"showed":[168],"maximum":[169],"viability":[170,203,219],"before":[171],"(83%)":[172],"after":[174],"(55%)":[175],"cryopreservation,":[176],"high":[177],"regrowth":[178,205,241],"percentage":[179,242],"(77%)":[180],"produced":[182],"average":[184],"9":[185],"colonies":[187],"per":[188],"Petri":[189],"dish.":[190],"Additionally,":[191],"dehydration":[192,237],"(1-5":[193],"h)":[194,235],"tested":[196,213],"reduce":[198],"water":[199],"content":[200],"improving":[202],"cryopreserved":[207,260,285,300],"cells.":[209],"Among":[210],"various":[212],"conditions,":[215],"highest":[217],"(72%)":[218],"observed":[221],"following":[222],"combination":[224,251],"treatments":[226],"(2":[231,234,238],"days),10%":[232],"h).":[239],"Maximum":[240],"(88%)":[243],"12":[245],"colonies/petri":[246],"dish":[247],"noted":[249],"+":[256],"PEG.":[258],"calli":[261],"differentiated":[262],"somatic":[264],"embryos":[265],"(52.78-54.33":[266],"globular":[267],"embryos/callus":[268],"mass)":[269],"NAA":[271],"(0.5":[272],"mg/l)":[273,277],"BAP":[275],"(0.5-1.0":[276],"media.":[279],"Plantlets":[280],"successfully":[282],"regenerated":[283],"2C":[289],"DNA":[290],"estimated":[292],"through":[293],"flow":[294],"cytometry.":[295],"genome":[297],"size":[298],"regenerant":[301],"1.51":[303],"pg/2C,":[304],"which":[305],"similar":[307],"field-grown":[309],"plants.":[311],"Vinblastine":[312],"vincristine":[314],"levels":[315],"nearly":[317],"same":[319],"mother":[321],"plant's":[322],"frozen":[324],"(cryopreserved)":[325],"leaf":[326],"tissue.":[327],"post":[329],"embryogenesis":[331],"protocol":[332],"may":[333],"be":[334],"continuous":[337],"production":[338],"plants":[340],"future":[342],"applications.":[343]},"counts_by_year":[{"year":2026,"cited_by_count":5},{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":2}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2022-10-06T00:00:00"}