{"id":"https://openalex.org/W4297900786","doi":"https://doi.org/10.7554/elife.80111.sa2","title":"Author response: Distinct regions of H. pylori\u2019s bactofilin CcmA regulate protein\u2013protein interactions to control helical cell shape","display_name":"Author response: Distinct regions of H. pylori\u2019s bactofilin CcmA regulate protein\u2013protein interactions to control helical cell shape","publication_year":2022,"publication_date":"2022-08-29","ids":{"openalex":"https://openalex.org/W4297900786","doi":"https://doi.org/10.7554/elife.80111.sa2"},"language":"en","primary_location":{"id":"doi:10.7554/elife.80111.sa2","doi":"https://doi.org/10.7554/elife.80111.sa2","is_oa":true,"landing_page_url":"https://doi.org/10.7554/elife.80111.sa2","pdf_url":null,"source":{"id":"https://openalex.org/S1336409049","display_name":"eLife","issn_l":"2050-084X","issn":["2050-084X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","jufo-2","ki-jl-2","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311710","host_organization_name":"eLife Sciences Publications Ltd","host_organization_lineage":["https://openalex.org/P4310311710"],"host_organization_lineage_names":["eLife Sciences Publications Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"peer-review"},"volume":null,"type":"peer-review","indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.7554/elife.80111.sa2","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5063161840","display_name":"Sophie R Sichel","orcid":"https://orcid.org/0000-0001-5079-189X","observed_orcids":["https://orcid.org/0000-0001-5079-189X"]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]},{"id":"https://openalex.org/I4210089486","display_name":"Fred Hutch Cancer Center","ror":"https://ror.org/007ps6h72","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I4210089486"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Sophie R Sichel","raw_affiliation_strings":["Division of Human Biology, Fred Hutchinson Cancer Center","Molecular Medicine and Mechanisms of Disease Graduate Program, University of Washington"],"raw_orcid":"https://orcid.org/0000-0001-5079-189X","affiliations":[{"raw_affiliation_string":"Division of Human Biology, Fred Hutchinson Cancer Center","institution_ids":["https://openalex.org/I4210089486"]},{"raw_affiliation_string":"Molecular Medicine and Mechanisms of Disease Graduate Program, University of Washington","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5036984709","display_name":"Benjamin P. Bratton","orcid":"https://orcid.org/0000-0003-1128-2560","observed_orcids":["https://orcid.org/0000-0003-1128-2560"]},"institutions":[{"id":"https://openalex.org/I200719446","display_name":"Vanderbilt University","ror":"https://ror.org/02vm5rt34","country_code":"US","type":"education","lineage":["https://openalex.org/I200719446"]},{"id":"https://openalex.org/I901861585","display_name":"Vanderbilt University Medical Center","ror":"https://ror.org/05dq2gs74","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210162197","https://openalex.org/I901861585"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Benjamin P Bratton","raw_affiliation_strings":["Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center","Vanderbilt Institute for Infection, Immunology and Inflammation"],"raw_orcid":"https://orcid.org/0000-0003-1128-2560","affiliations":[{"raw_affiliation_string":"Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center","institution_ids":["https://openalex.org/I901861585"]},{"raw_affiliation_string":"Vanderbilt Institute for Infection, Immunology and Inflammation","institution_ids":["https://openalex.org/I200719446"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5013051962","display_name":"Nina R. Salama","orcid":"https://orcid.org/0000-0003-2762-1424","observed_orcids":["https://orcid.org/0000-0003-2762-1424"]},"institutions":[{"id":"https://openalex.org/I4210089486","display_name":"Fred Hutch Cancer Center","ror":"https://ror.org/007ps6h72","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I4210089486"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Nina R Salama","raw_affiliation_strings":["Division of Human Biology, Fred Hutchinson Cancer Center"],"raw_orcid":"https://orcid.org/0000-0003-2762-1424","affiliations":[{"raw_affiliation_string":"Division of Human Biology, Fred Hutchinson Cancer Center","institution_ids":["https://openalex.org/I4210089486"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3000,"currency":"USD","value_usd":2500},"apc_paid":{"value":3000,"currency":"USD","value_usd":2500},"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10276","display_name":"Helicobacter pylori-related gastroenterology studies","score":0.8032000064849854,"subfield":{"id":"https://openalex.org/subfields/2746","display_name":"Surgery"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10276","display_name":"Helicobacter pylori-related gastroenterology studies","score":0.8032000064849854,"subfield":{"id":"https://openalex.org/subfields/2746","display_name":"Surgery"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.155799999833107,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13326","display_name":"Biochemical and Structural Characterization","score":0.012199999764561653,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/helicobacter-pylori","display_name":"Helicobacter pylori","score":1.0},{"id":"https://openalex.org/keywords/ccma","display_name":"CCMA","score":0.9549999833106995},{"id":"https://openalex.org/keywords/bactofilin","display_name":"bactofilin","score":0.9190000295639038},{"id":"https://openalex.org/keywords/protein-protein-interaction","display_name":"protein-protein interaction","score":0.8050000071525574},{"id":"https://openalex.org/keywords/cell-shape-regulation","display_name":"cell shape regulation","score":0.4009999930858612}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.2910580039024353}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.7554/elife.80111.sa2","doi":"https://doi.org/10.7554/elife.80111.sa2","is_oa":true,"landing_page_url":"https://doi.org/10.7554/elife.80111.sa2","pdf_url":null,"source":{"id":"https://openalex.org/S1336409049","display_name":"eLife","issn_l":"2050-084X","issn":["2050-084X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","jufo-2","ki-jl-2","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311710","host_organization_name":"eLife Sciences Publications Ltd","host_organization_lineage":["https://openalex.org/P4310311710"],"host_organization_lineage_names":["eLife Sciences Publications Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"peer-review"}],"best_oa_location":{"id":"doi:10.7554/elife.80111.sa2","doi":"https://doi.org/10.7554/elife.80111.sa2","is_oa":true,"landing_page_url":"https://doi.org/10.7554/elife.80111.sa2","pdf_url":null,"source":{"id":"https://openalex.org/S1336409049","display_name":"eLife","issn_l":"2050-084X","issn":["2050-084X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","jufo-2","ki-jl-2","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311710","host_organization_name":"eLife Sciences Publications Ltd","host_organization_lineage":["https://openalex.org/P4310311710"],"host_organization_lineage_names":["eLife Sciences Publications Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"peer-review"},"sustainable_development_goals":[{"score":0.4000000059604645,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4294992058","https://openalex.org/W4293736719","https://openalex.org/W7123274742","https://openalex.org/W4292860113","https://openalex.org/W2883113049","https://openalex.org/W2999357272","https://openalex.org/W2943048528","https://openalex.org/W2073924276","https://openalex.org/W2947492963","https://openalex.org/W2557834245"],"abstract_inverted_index":{"Article":[0,20],"Figures":[1],"and":[2,11,21,76,78,113,124,135,145,149,171,189,204,213,269,341,373,383,420,471,484,519,531,543,567,579,709,778,830,835,851,873,876,900,918,924,931,997,1009,1078,1146,1172,1216,1228,1286,1296,1343,1349,1380,1443,1535,1550,1569,1631,1637,1681,1693,1732,1827,1840,1882,1933,1964,1970,2016,2025,2034,2160,2171,2188,2196,2213,2223,2229,2274,2301,2339,2366,2393,2399,2405,2449,2464,2483,2489,2520,2548,2559,2614,2620,2625,2632,2636,2658,2667,2672,2679,2710,2726,2733,2751,2775,2824,2903,2923,2932,2939,2984,2986,3006,3044,3059,3075,3094,3122,3162,3201,3224,3302,3316,3329,3348,3357,3393,3412,3414,3433,3496,3503,3617,3630,3636,3718,3736,3744,3796,3807,3814,3874,3893,3916,3944,4004,4039,4298,4311,4335,4338,4345,4385,4442],"data":[3,1831,2736,2803,3596,3621,3784,3817,4112,4150,4281,4302,4453],"Abstract":[4,25],"Editor's":[5,224],"evaluation":[6,225],"Introduction":[7,311],"Results":[8,989],"Discussion":[9],"Materials":[10],"methods":[12],"Data":[13,1811,2913,3591,4107,4168,4276],"availability":[14],"References":[15],"Decision":[16,303],"letter":[17,304],"Author":[18],"response":[19],"author":[22],"information":[23],"Metrics":[24],"The":[26,360,772,1060,1340,1403,1560,1684,2332,2492,2596,2842,3406],"helical":[27,40,100,132,196,222,240,275,361,408,590,609,861,912,953,1354,1397,2069,2383,2420,2528,2818,2833,3019,4475],"shape":[28,41,101,134,237,300,362,409,419,683,862,913,955,1355,1398,2319,2373,2385,2535,2819,3021,3925],"of":[29,42,69,102,127,139,166,282,295,324,345,363,370,410,415,422,500,537,572,592,602,619,625,648,661,711,727,733,756,774,781,826,833,855,863,914,935,985,993,1006,1019,1046,1065,1074,1084,1136,1170,1180,1184,1188,1203,1232,1256,1262,1288,1300,1308,1317,1336,1356,1366,1391,1399,1414,1450,1466,1515,1564,1567,1572,1588,1602,1609,1644,1679,1718,1725,1743,1750,1767,1774,1814,1846,1853,1864,1908,1944,1957,1986,2005,2013,2042,2063,2086,2118,2129,2133,2142,2162,2175,2220,2335,2345,2382,2413,2419,2432,2440,2447,2452,2471,2498,2503,2525,2553,2557,2603,2660,2692,2746,2754,2763,2769,2778,2797,2808,2820,2845,2866,2880,2890,2896,2916,2928,2990,3001,3009,3018,3031,3155,3177,3186,3237,3249,3256,3286,3367,3448,3460,3472,3484,3511,3523,3531,3542,3554,3574,3588,3595,3680,3710,3713,3760,3790,3801,3822,3867,3903,3954,3963,3973,3980,3999,4002,4007,4055,4071,4081,4099,4111,4120,4202,4217,4229,4246,4261,4273,4280,4317,4324,4364,4413,4435],"Helicobacter":[30,312],"pylori":[31,44,104,313,336,353,365,412,594,642,865,916,1150,1358,1401,1460,1667,1720,1745,1769,1848,1978,2112,2379,2822,3323,3439,3500,3957,4194,4319,4407],"cells":[32,45,366,595,866,917,1359,1668,1979,2015,2031,2037,2120,2823,3440,3918,4195],"promotes":[33,190,374],"robust":[34,3291],"stomach":[35,372],"colonization;":[36],"however,":[37,1875],"how":[38,736,847,887,950,1388,3152],"the":[39,99,121,136,163,173,186,194,216,233,245,264,274,293,325,371,407,418,423,465,476,493,528,588,616,620,635,640,741,746,754,775,790,824,827,848,852,860,911,936,982,1015,1069,1079,1117,1160,1163,1167,1173,1177,1185,1209,1222,1225,1229,1254,1267,1281,1289,1298,1301,1309,1312,1318,1323,1328,1337,1353,1396,1408,1415,1447,1469,1480,1491,1497,1508,1513,1516,1527,1541,1547,1565,1570,1586,1596,1600,1642,1645,1703,1764,1861,1879,1890,1900,1911,1950,1960,1968,1994,2011,2014,2021,2039,2051,2058,2064,2078,2083,2108,2115,2130,2137,2143,2157,2210,2224,2239,2257,2283,2315,2370,2387,2407,2414,2500,2662,2739,2749,2761,2767,2776,2794,2806,2817,2854,2888,2987,2998,3016,3028,3189,3195,3227,3234,3257,3262,3283,3304,3325,3374,3384,3415,3528,3571,3669,3683,3695,3711,3757,3771,3787,3798,3825,3844,3864,3911,3921,3955,3960,4000,4005,4019,4022,4052,4104,4124,4131,4149,4157,4160,4165,4361,4391,4410,4432,4473],"H.":[43,103,335,352,364,411,593,641,864,915,951,1149,1357,1400,1459,1666,1719,1744,1768,1847,1977,2111,2378,2800,2821,3322,3438,3499,3956,4193,4318,4406],"is":[46,48,141,244,314,455,514,522,614,634,748,766,838,956,991,1044,1063,1072,1082,1284,2280,2396,2409,2438,2496,2742,2812,2847,2883,3269,3288,3387,3976,4049,4057,4425,4437,4458],"determined":[47,1898],"unresolved.":[49],"Previous":[50],"work":[51],"identified":[52,119,402,752,886,919,3281],"helical-cell-shape-promoting":[53],"protein":[54,256,452,458,525,574,881,888,933,967,983,1242,1947,2138,2408,3859],"complexes":[55,3811],"containing":[56],"a":[57,60,66,79,221,239,248,254,259,315,456,523,533,598,608,646,659,737,757,763,946,986,994,1023,1047,1095,1195,1241,1271,1276,1293,1334,1373,1377,1381,1504,1523,1537,1606,1613,1737,1919,2272,2417,2526,2773,3086,3135,3319,3331,3732,3752,4032,4089],"peptidoglycan-hydrolase":[58,174],"(Csd1),":[59],"peptidoglycan":[61,205,267,424],"precursor":[62,489],"synthesis":[63,206,268,285,585,627,732],"enzyme":[64,490],"(MurF),":[65],"non-enzymatic":[67,72],"homolog":[68,536],"Csd1":[70,1589,3857,3904,3939,4026,4047,4121,4140,4185,4356,4388,4450,4456],"(Csd2),":[71],"transmembrane":[73,457,524],"proteins":[74,203,399,449,654,806,903,1972,2172,2356,2517],"(Csd5":[75],"Csd7),":[77],"bactofilin":[80,122,137,249,637,739,758,779,828,849,995,1025,1048,1070,1161,1186,1210,1226,1268,1302,1324,1338,1344,1378,1404,1416,1542,1689,2267,2284,2493,2740,2843,2978,3385,3407,3788],"(CcmA).":[81],"Bactofilins":[82,644,750],"are":[83,129,349,404,569,645,751,783,904,1346,1698,1812,1973,1989,2357,2686,2914,2942,3106,3441,3593,3828,3838,4028,4109,4169,4196,4278,4417,4483],"highly":[84,649],"conserved,":[85,650],"spontaneously":[86,651],"polymerizing":[87],"cytoskeletal":[88,255,653],"bacterial":[89,687],"proteins.":[90,2163,3047],"We":[91,151,894,958,1473,1917,2185,2542,3210,3343,3766,4381,4402],"sought":[92,4369],"to":[93,185,208,219,272,292,351,462,473,587,800,822,845,858,909,944,1252,1351,1386,1394,1423,1583,1712,1905,1983,2155,2202,2208,2286,2313,2562,2600,2638,2646,2705,2728,2780,2810,2815,2887,3052,3099,3158,3231,3334,3371,3421,3428,3490,3526,3557,3569,3633,3665,3720,3833,3891,3897,3987,4060,4130,4183,4189,4249,4341,4370,4421,4472,4485],"understand":[94],"CcmA\u2019s":[95,155,907,1007,2266,2977],"function":[96,773,825,1587,3993],"in":[97,110,114,238,262,286,464,475,615,639,655,684,745,796,963,1030,1109,1124,1159,1206,1421,1715,1753,1843,1876,1976,2261,2377,2402,2594,2723,2799,2825,2850,2885,3011,3033,3091,3134,3321,3437,3498,3576,3640,3668,3727,3769,3855,3951,4018,4031,4123,4187,4192,4314,4347,4390,4405,4419],"generating":[98,859],"cells.":[105,1402,2046],"Using":[106,176],"CcmA":[107,128,140,167,184,191,199,474,503,544,578,613,633,834,856,871,892,899,923,929,961,990,1020,1043,1110,1125,1147,1189,1204,1363,1392,1670,1680,1714,1726,1751,1775,1865,1909,1932,1934,1958,1987,2006,2087,2100,2134,2191,2222,2298,2303,2316,2348,2362,2391,2395,2403,2435,2437,2453,2473,2504,2509,2558,2577,2702,2747,2755,2779,2798,2809,2846,2930,2991,3002,3032,3043,3048,3156,3238,3250,3287,3392,3495,3635,3661,3681,3700,3714,3739,3742,3791,3802,3808,3823,3868,3877,3887,4036,4056,4365,4424,4436,4462,4465],"deletion":[108,3709],"analysis,":[109],"vitro":[111,2826,2851],"polymerization,":[112,798],"vivo":[115],"co-immunoprecipitation":[116,549,3062,3070,3092,3128,3166,3332,3641,3725,4348,4398],"experiments,":[117,1896],"we":[118,179,841,885,1013,1234,1384,1444,1457,1604,1626,1884,1897,2009,2124,2291,2306,2530,2792,3013,3023,3026,3164,3243,3280,3311,3647,3650,3722,3773,3883,3937,4015,4368,4393],"that":[120,154,181,247,319,403,459,491,526,551,581,612,657,765,789,896,920,960,973,1042,1092,1246,1327,1362,1370,1407,1462,1544,1628,1899,1967,2001,2018,2048,2082,2099,2110,2173,2265,2276,2322,2354,2512,2582,2627,2682,2697,2717,2738,2759,2771,2805,2882,2997,3015,3103,3182,3271,3282,3345,3373,3382,3694,3755,3768,3786,3820,3863,3875,3885,3913,3984,4017,4064,4355,4404,4430,4455,4481],"domain":[123,138,470,759,780,829,850,996,1049,1071,1187,1211,1227,1269,1303,1345,1417,1543,1690,2268,2285,2494,2741,2844,2979,3386,3408,3530,3573,3759,3789],"N-terminal":[125,156,164,1061,1102,1168,1213,1282,1319,1329,1341,1368,1481,1548,1685,2022,2065,2084,2116,2988,3029,3416,3684,3696,3799,3865,3961,4023,4053,4362,4411,4433],"region":[126,157,165,618,1062,1081,1169,1179,1214,1218,1283,1291,1320,1330,1342,1369,1390,1482,1529,1549,1552,1686,1695,1962,2023,2041,2066,2085,2117,2279,2514,2879,2989,3030,3154,3285,3417,3685,3697,3800,3827,3866,3962,4024,4054,4363,4412,4434],"required":[130,218,2281,3289,3388],"for":[131,143,298,406,906,949,1617,2282,2688,2744,2849,3261,3290,3389,3746,3989,4145,4387,4469],"cell":[133,187,210,236,283,299,621,682,712,743,869,954,1010,1454,1675,2318,2372,2384,2421,2534,2834,3020],"sufficient":[142,1350,2743,2814,2848],"polymerization":[144,872,1430,2540,2544,2745,2852,3010],"interactions":[146,170,501,540,897,921,2507,2758,2981,2993,3041,3065,3080,3105,3159,3336,3390,3431,3435,3493,3747,3850,3871,4063,4191,4374,4379,4416,4440],"with":[147,160,290,334,467,481,502,597,804,878,1021,1152,1275,1322,1657,1728,1838,1870,1923,2256,2538,2785,2832,2982,2994,3054,3160,3216,3226,3277,3308,3318,3337,3379,3397,3410,3456,3468,3480,3494,3502,3519,3538,3550,3565,3584,3628,3748,3778,3794,3805,3812,3872,3946,4075,4093,4206,4221,4233,4242,4257,4269,4309,4333],"Csd5":[148,182,454,478,568,2983,3058,3121,3161,3411,3497,3575,3719,3749,3761,3775,3795,3813],"Csd7.":[150,161,3815,4464],"also":[152,479,2292,3818],"found":[153,180,895,2017,2722,3344,3651,3767,4016,4403],"inhibits":[158,2992,3418,3698,3803],"interaction":[159,877,882,3793,3804],"Deleting":[162],"increases":[168],"CcmA-Csd7":[169,3849,4439],"destabilizes":[172],"Csd1.":[175,538,938],"super-resolution":[177],"microscopy,":[178],"recruits":[183],"envelope":[188],"enrichment":[192],"at":[193,1468,1512,1611,1980,2180,2205,2231,2243,2299,2330,2341,2349,2454,2474,3188,3194,3206,3324,3352,3830,3982,4103],"major":[195,589,4474],"axis.":[197],"Thus,":[198,1456,2360],"helps":[200,367],"organize":[201],"cell-shape-determining":[202,447,3046],"machinery":[207],"coordinate":[209],"wall":[211,284,744],"modification":[212,730],"synthesis,":[214],"promoting":[215],"curvature":[217,600,1766,2075],"build":[220],"cell.":[223],"This":[226,1039,3906],"study":[227],"addresses":[228],"an":[229,486,1367,1871,3947,3977,4096],"important":[230],"unresolved":[231],"question,":[232],"mechanisms":[234],"governing":[235],"organism.":[241],"Particularly":[242],"exciting":[243],"observation":[246],"\u2013":[250,257,2380,2386],"typically":[251],"viewed":[252],"as":[253,1700,1702,1736,2665,3198,3355,3653,3841,4088,4210],"plays":[258],"key":[260],"role":[261],"modulating":[263,681,797,981,1425,1429,2766],"balance":[265],"between":[266,541,898,922,1141,1224,2508,3042,3120,3391],"degradation":[270],"necessary":[271,1348],"generate":[273],"shape.":[276,1455,2422,2835],"These":[277,2735,3364],"findings":[278,941],"enhance":[279],"our":[280,1924,2786,3294,4396],"understanding":[281],"bacteria":[287,318,656],"generally,":[288],"particularly":[289],"regard":[291],"importance":[294],"local":[296],"phenomena":[297],"generation.":[301],"https://doi.org/10.7554/eLife.80111.sa0":[302],"Reviews":[305],"on":[306,583,607,1411,1889,1928,2296,2584,3172],"Sciety":[307],"eLife's":[308],"review":[309],"process":[310],"helical-shaped,":[316],"Gram-negative":[317],"infects":[320],"more":[321,2629],"than":[322,2077,2634,2700,2720,4030],"half":[323],"world\u2019s":[326],"population":[327],"(Hooi":[328],"et":[329,357,379,387,391,395,431,435,439,443,505,509,560,630,664,669,673,677,690,694,698,702,706,715,719,723,808,812,816,1036,1112,1238,1432,1436,1440,1579,1620,1648,1652,2327,2569,2573,3112,3116,3143,3147,3657,3763,3930],"al.,":[330,358,380,388,392,396,432,436,440,444,506,510,561,631,665,670,674,678,691,695,699,703,707,716,720,724,809,813,817,1037,1113,1239,1433,1437,1441,1580,1621,1649,1653,2328,2570,2574,3113,3117,3144,3148,3658,3764,3931],"2017).":[331],"Chronic":[332],"infection":[333,354,377],"can":[337,975,1633,2152,3274,3377],"cause":[338],"gastric":[339,346],"ulcers":[340],"cancers;":[342],"approximately":[343,2604,2661],"80%":[344],"cancer":[347],"cases":[348],"attributed":[350],"(de":[355],"Martel":[356],"2020).":[359,632],"establish":[368,945],"colonization":[369],"immunopathology":[375],"during":[376,4395],"(Mart\u00ednez":[378],"2019;":[381,717,810,1434,2571],"Montecucco":[382],"Rappuoli,":[384],"2001;":[385],"Sycuro":[386,390,434,438,705],"2010;":[389,433,814,1438,1650],"2012;":[393,441],"Yang":[394,442,1651,3115,3146,3929],"2019).":[397,445,1114,1654,3118,3149,3932],"Multiple":[398],"have":[400,545,1463,1673,2089,2167,2177,2238,2307,2369],"been":[401,546,787,1090,4479],"essential":[405,487],"cells,":[413,1780,1785,1790,1795,1800,1805],"most":[414],"which":[416,728,761,1104,1193],"influence":[417,976,2140],"composition":[421],"(PG)":[425],"sacculus":[426,978],"directly":[427,461],"or":[428,731,1428,2057,3108,3137,3204,3214,3638,3741],"indirectly":[429,4459],"(Sycuro":[430,1578,1647],"2013;":[437,666],"Several":[446],"(Csd)":[448],"form":[450,2601,2615,2647,2706,2729,2772,2781],"membrane-spanning":[451],"complexes.":[453,968],"binds":[460,472],"PG":[463,488,496,584,626,742,977,987],"periplasm":[466,747],"its":[468,925,2215,3895],"SH3":[469,3529,3572,3758],"cytoplasm.":[477],"interacts":[480,3307,3409],"ATP":[482],"synthase":[483],"MurF,":[485],"catalyzes":[492],"final":[494],"cytoplasmic":[495,738],"biosynthesis":[497],"step,":[498],"independent":[499,1816,2918,3599,4115,4173,4284],"(Blair":[504,3111,3762],"2018;":[507,700,3114,3141,3145],"Hrast":[508],"2013).":[511],"Another":[512],"complex":[513,575],"formed":[515,1158,2623,2668,2695,2714],"by":[516,548,556,753,768,867,980,1053,1191,1575,1590,1867,1993,2259,2545,2765,3039,3061,3069,3072,3127,3131,3169,3253,3293,3362,3672,3689,3704,3750,3843,3881,3910,3941,4010,4138,4142,4153,4156,4461],"Csd7,":[517,566,2985,3060,3163,3413,3717,3797,3806,3882],"Csd1,":[518,530,3899,4344,4447],"Csd2.":[520],"Csd7":[521,542,2995,3123,3419,3690,3779,3873,3892,4444],"stabilizes":[527],"PG-hydrolase":[529,937],"Csd2,":[532],"catalytically":[534],"inactive":[535],"Weak":[539],"detected":[547,3068,3084,3126,3252,3667],"experiments":[550,2787,3093,3129,3167,3427,3489,3726,4182,4399],"include":[552],"cross-linking":[553,3087],"reagents":[554],"followed":[555,3071,3130,3168],"mass":[557,3073,3095,3132],"spectrometry":[558,3074,3096,3133],"(Yang":[559,3656],"2019),":[562,3659],"but":[563,1988,2070,2411,2703],"whether":[564,1116,2127,2532,3233,3708,4372],"CcmA,":[565,1171,1233,1603,2119,2655,2870,2897,3101,3314,3716,3731,3735,3835,4343,4414],"all":[570,1265,1661,2002,2233,2368,3246,3401,3770,3919],"part":[571,1287],"one":[573,1477],"was":[576,1122,1126,1364,3049,3089,3097,3662,3878,3908,3985,4086,4101,4136,4151,4366],"unclear.":[577,749],"probes":[580],"report":[582],"localize":[586],"axis":[591,4476],"(regions":[596],"Gaussian":[599],"value":[601,4158],"5":[603,3396],"\u00b1":[604],"1":[605,1129,1759,1832,3398,3622,4303],"\u00b5m\u20132":[606],"cell),":[610],"indicating":[611,2246,2321],"same":[617,2371,2663,3922,4166],"where":[622,2791],"increased":[623,4061],"levels":[624,1982,3354,3832,3860,3902,4027,4080,4122,4186,4357,4389],"occur":[628],"(Taylor":[629,2326],"only":[636,1540,2639,3082,3383],"encoded":[638],"genome.":[643],"class":[647],"polymerizing,":[652],"perform":[658],"variety":[660],"functions":[662],"(Bulyha":[663],"El":[667],"Andari":[668],"2015;":[671],"Lin":[672],"2017;":[675],"Osorio-Valeriano":[676],"2019)":[679],"including":[680],"multiple":[685,2090],"different":[686,1674,2073],"species":[688],"(Brockett":[689],"2021;":[692],"Hay":[693],"1999;":[696],"Jackson":[697],"Koch":[701],"2011;":[704],"2010)":[708],"morphogenesis":[710],"appendages":[713],"(Billini":[714],"Caccamo":[718],"2020;":[721],"K\u00fchn":[722,811,1435],"2010),":[725,1581],"both":[726,1546,1629,2336,3346],"involve":[729],"PG.":[734],"But":[735],"influences":[740],"presence":[755],"(DUF583),":[760],"forms":[762,1194,1270,1292,2271,3809],"\u03b2-helix":[764,1274,2273],"flanked":[767],"unstructured":[769],"terminal":[770,776,791,831,853,1000,1056,1230,1409,1448,2131,2278,2501,2513,2855],"regions.":[771],"regions":[777,792,832,854,1001,1057,1231,1410,1449,2092,2132,2502,2753,2856],"bactofilins":[782,837,974,1093,4482],"debated.":[784],"It":[785,1088,4477],"has":[786,1089,4478],"proposed":[788,1091,1118,1492],"may":[793,1371,1418,2088],"be":[794,1419,1634,2153,3037,3083,3359,3666,3889],"involved":[795,1029,1420],"binding":[799,889,926,1098,1120,1375,1422,1494,1706,2054,3420,3890,4467],"membranes":[801],"and/or":[802,2139],"interacting":[803],"other":[805,836,901,1646,1984,3045],"(Deng":[807,1111,1431],"Vasa":[815,1439],"2015).":[818],"However,":[819,1115,3078,3678],"experimental":[820],"evidence":[821,3368],"identify":[823,1387,2156,2209,3232,3707,4371],"lacking.":[839],"Here,":[840],"performed":[842,3165,3221,3330,4382],"structure\u2013function":[843,1004],"analysis":[844,1005],"determine":[846],"contribute":[857],"evaluating":[868],"morphology,":[870],"filament":[874,2689],"bundling,":[875],"known":[879],"Csd":[880,902,3056],"partners.":[883],"Additionally,":[884,969,3310],"partners":[890,927,4468],"impact":[891,2760],"localization.":[893],"responsible":[905,2687],"ability":[908,2777,2807],"pattern":[910,1352,2816],"modulate":[928,932,2505],"localization":[930,4471],"stability":[934,984,4457],"Together,":[939,2252,3782],"these":[940,970,2253,2355,2802,3783],"allow":[942],"us":[943,3370],"new":[947],"model":[948],"pylori\u2019s":[952],"generated.":[957],"clarify":[959],"participates":[962],"two":[964,998,1054,1815,2302,2484,2907,2917,3055,3971,4283],"separate":[965,3810],"helical-cell-shape-generating":[966],"studies":[971],"reveal":[972],"structure":[979,1135,1243,1255,2106,2159,2212,2249],"hydrolase.":[988],"composed":[992,1045,1064,1073,1083,1365,2439],"short":[999,1055,1096,1294],"To":[1002,3150,3706,3933],"facilitate":[1003],"biochemical":[1008],"biologic":[1011],"functions,":[1012],"aligned":[1014],"amino":[1016,1066,1075,1085,1106,1315,1485,1501,1510,1520,1532,1555,1593,2061,2683],"acid":[1017],"sequence":[1018],"BacA,":[1022],"well-studied":[1024],"from":[1026,1143,1148,1595,2029,2200,2788,3597,3701,4113,4159,4164,4171,4282,4445],"Caulobacter":[1027],"crescentus":[1028,1145],"stalk":[1031],"morphogenesis,":[1032],"using":[1033,3751],"SWISS-MODEL":[1034],"(Waterhouse":[1035],"2018).":[1038,3765],"alignment":[1040,1140],"revealed":[1041],"(DUF":[1050],"583)":[1051],"surrounded":[1052],"(Figure":[1058,1279,1304,1558,1892,1913,1937,2032,2146,2192,2250,2351,2618,2650,2670,2677,2708,2731,3265,3298,3339,3691,3780,3927,4037,4400,4427],"1A).":[1059],"acids":[1067,1076,1086,1107,1316,1486,1502,1511,1521,1533,1556,1594,2062,2684],"1\u201317,":[1068],"18\u2013118,":[1077],"C-terminal":[1080,1178,1217,1290,1382,1528,1551,1694,1961,2040,2752,3284,3826],"119\u2013136.":[1087],"contain":[1094,1372,2640],"membrane":[1097,1119,1374,1493,1705,2053],"motif":[1099,1121,1495,1707,2055],"within":[1100,1496,2093],"their":[1101,2533,3207],"regions,":[1103],"spans":[1105],"3\u201312":[1108],"functional":[1123,2091],"unknown.":[1127],"Figure":[1128,1655,1829,2262,2423,2789,2836,3034,3395,3619,4041,4300],"Download":[1130,1662,1856,2425,2838,3402,3644,4043,4351],"asset":[1131,1133,1663,1665,2426,2428,2839,2841,3403,3405,4044,4046],"Open":[1132,1664,2427,2840,3404,4045],"Predicted":[1134],"CcmA.":[1137,1181,1257,1263,1854,3178,3422,3505,4325],"(A)":[1138,1677,2443,3432,4068],"Sequence":[1139],"BacA":[1142],"C.":[1144],"generated":[1151,1190,1205,1259,1458,1474,3181],"SWISS-MODEL;":[1153],"gray":[1154],"boxes":[1155,2877],"indicate":[1156,2878,2894,2900,4095],"\u03b2-strands":[1157,2145,2176],"domain,":[1162,1325,1379],"green":[1164],"box":[1165,1175],"indicates":[1166,1176,2906],"pink":[1174],"(B)":[1182,1709,2467,3436,4118],"Model":[1183],"RoseTTAFold,":[1192],"right-handed,":[1196],"three-sided,":[1197,1272],"triangular":[1198],"\u03b2-helix.":[1199],"(C)":[1200,1740,3487,4179],"Five":[1201],"models":[1202,1261],"RoseTTAFold":[1207,1236,1258,2260],"displaying":[1208,1763,2926],"(gray),":[1212],"(green),":[1215,2461],"(pink).":[1219],"After":[1220,1360,3861,4353],"identifying":[1221,1361,4354],"boundaries":[1223],"used":[1235,1616,2154,3098,4087],"(Baek":[1237],"2021),":[1240],"prediction":[1244],"service":[1245],"uses":[1247],"deep":[1248],"learning-based":[1249],"modeling":[1250,2258],"methods,":[1251],"predict":[1253],"five":[1260,1310,1475,1509,2060],"In":[1264,1306,1623,1894,2289,2390,4326],"five,":[1266],"right-handed":[1273],"left-handed":[1277],"twist":[1278],"1B),":[1280],"unstructured,":[1285],"\u0251-helix":[1295],"caps":[1297],"end":[1299,1413,1514,1566,4001],"1C).":[1305],"four":[1307],"models,":[1311],"first":[1313],"several":[1314,1592],"interact":[1321,3053,3276,3378],"suggesting":[1326,1966,2081,2353,2681,3102,3381,3693,4429],"could":[1331,3036,3081,3239,3358,3869,3888],"potentially":[1332,3109,3990],"block":[1333],"surface":[1335],"domain.":[1339],"together":[1347,2599,2784],"motif,":[1376],"region,":[1383],"wanted":[1385],"each":[1389,1945,2911,3153,4146],"contributed":[1393],"patterning":[1395],"literature":[1405],"suggested":[1406],"either":[1412,2050,3202,3212],"membranes,":[1424],"protein\u2013protein":[1426],"interactions,":[1427,3853],"2015),":[1442],"hypothesized":[1445,3014,4480],"deleting":[1446],"ccmA":[1451,1467,1471,1573,1630,2043,3187,3191,3327,3996,4008,4125],"would":[1452],"alter":[1453],"strains":[1461,1721,1746,1770,1849,1859,1877,2019,2113,3173,3501,3728,3737,4320,4408],"truncated":[1464,1723,1748,1772,1851,1862,2003,2450,2555,2795,2999,3175,3184,3235,4322],"versions":[1465,1724,1749,1773,1852,1863,1907,1956,1985,2004,2451,2556,2796,3000,3176,3185,3236,3248,3679,3821,4323],"native":[1470,3190,3208,3326],"locus.":[1472,4106],"strains,":[1476],"strain":[1478,1489,1506,1525,1538,3733,3958,3969,4020,4033,4128,4163],"missing":[1479,1484,1490,1500,1526,1531],"(\u2206NT,":[1483],"2\u201317),":[1487],"another":[1488],"N-terminus":[1498,1517,1601,2415,3712],"(\u2206MM,":[1499],"3\u201312),":[1503],"third":[1505],"lacking":[1507,1519,1878,1910,1959,2020,2038,2114,3682,3734,3824,3959,4021,4409],"(\u220613\u201317,":[1518],"13\u201317),":[1522],"fourth":[1524],"(\u2206CT,":[1530],"119\u2013136),":[1534],"lastly,":[1536],"expressing":[1539,1554,1669,1722,1747,1771,1850,3174,3729,3738,4034,4321],"lacks":[1545],"(BD,":[1553,1691],"18\u2013118)":[1557],"2A).":[1559],"open-reading":[1561],"frames":[1562],"(ORFs)":[1563],"csd1":[1568,1610,1632,3193,3981,4003,4100],"beginning":[1571,4006],"overlap":[1574,4009],"82":[1576,4011],"nucleotides":[1577],"thus":[1582],"avoid":[1584],"impacting":[1585,1641,3991],"removing":[1591],"C-terminus":[1597,1880,1912],"when":[1598,2375,3025,3085,3994,4051,4360,4377,4422,4431],"truncating":[1599,3995],"expressed":[1605,1860,1975,2187,2376,2793,3183,3351,3829,4426],"second":[1607],"copy":[1608,3979,4098],"rdxA,":[1612],"locus":[1614,3197,3328],"commonly":[1615],"complementation":[1618],"(Smeets":[1619],"2000).":[1622],"previous":[1624],"work,":[1625],"confirmed":[1627],"individually":[1635],"deleted":[1636,3027,4058,4438],"complemented":[1638],"back":[1639],"without":[1640,3504],"expression":[1643,3940,4048],"2":[1656,1658,3035],"supplements":[1659],"see":[1660,3400],"truncation":[1671,1682,1935,2101,2225,2515],"mutants":[1672,1936,2102,2226,2305,2337,2486],"shapes.":[1676],"Schematic":[1678],"mutants.":[1683],"(NT,":[1687],"green),":[1688],"cyan),":[1692],"(CT,":[1696],"pink)":[1697],"indicated,":[1699],"well":[1701],"putative":[1704,2052],"(MM).":[1708],"Western":[1710,1835,1868,1920,3076,3170,3222,3254,3453,3465,3477,3516,3535,3547,3562,3581,3625,3673,3942,4069,4200,4215,4227,4239,4254,4266,4306,4330,4383],"blot":[1711,1891,1921,3454,3466,3478,3517,3536,3548,3563,3582,4070,4201,4216,4228,4240,4255,4267],"detect":[1713,3100,3335,3527,3558,3570,3634,4250,4342],"whole-cell":[1716,1844,3952,4072,4315],"lysates":[1717,1845,3953,4316],"probed":[1727,1837,1922,3225,3455,3467,3479,3518,3537,3549,3564,3583,3627,3745,4074,4205,4220,4232,4241,4256,4268,4308,4332,4386],"\u0251-CcmA":[1729,1841,1872,1901,1926,1952,1995,3228,3295,3457,3481,3539,3585,3629,3845,4234,4270,4334],"polyclonal":[1730,1734,1873,1902,1925,1951,1996,3229,3296,3458,3482,3521,3540,3567,3586,3753,3846,3949,4077,4084,4208,4223,4235,4259,4271],"antibody,":[1731],"\u0251-Cag3":[1733,1839,4083,4207,4310,4337],"antibody":[1735,1903,1927,1953,3230,3297,3459,3471,3483,3522,3541,3553,3568,3587,3754,3950,4078,4085,4209,4245,4260,4272],"loading":[1738,4090,4211],"control.":[1739,4091,4212],"Phase-contrast":[1741],"images":[1742],"diagramed":[1752],"panel":[1754,2886],"(A),":[1755],"scale":[1756,2871],"bars":[1757,2872],"=":[1758,1778,1783,1788,1793,1798,1803,1808,2589,2592,2608,2611,2873,2955,2959,2963,2967,2971,2975],"\u00b5m.":[1760],"(D)":[1761],"Histogram":[1762],"side":[1765,2074],"(WT":[1776],"n":[1777,1782,1787,1792,1797,1802,1807,2591,2610,2954,2958,2962,2966,2970,2974],"476":[1779],"\u2206ccmA":[1781,2030,2122,3138],"397":[1784],"\u2206N":[1786],"275":[1789],"\u2206MM":[1791,2652,2961],"391":[1794],"\u220613\u201317":[1796,2674,2965],"361":[1799],"\u2206CT":[1801,1969,2462,2694,2969,3263,3375],"263":[1804],"BD":[1806,1971,2230,2465,2713,2973,3305,3317],"315":[1809],"cells).":[1810],"representative":[1813,2915,3594,4110,4279],"biological":[1817,2919,3600,4116,4174,4285],"replicates.":[1818,2920,3601,4117,4175,4286],"Strains":[1819,3179,3602,4092,4287],"used:":[1820,3603,4288],"SSH51A,":[1821,4293],"SSH53A,":[1822,4294],"SSH55A,":[1823,4295],"SSH54A,":[1824],"SSH67A,":[1825],"SSH56B,":[1826],"SSH57A.":[1828],"2\u2014source":[1830],"Raw,":[1833,3623,4304],"unedited":[1834,3624,4305,4329],"blots":[1836,3626,4307,4331],"antibodies":[1842,3632,4313,4340],"https://cdn.elifesciences.org/articles/80111/elife-80111-fig2-data1-v2.zip":[1855],"elife-80111-fig2-data1-v2.zip":[1857],"All":[1858],"measured":[1866],"blotting":[1869,3171,3223,3255,3674,3943,4384],"antibody;":[1874],"(\u2206CT":[1881,1963],"BD),":[1883,1965],"saw":[1885],"much":[1886],"lower":[1887,4029],"signal":[1888,4135,4141,4144],"2B).":[1893],"follow-up":[1895],"fails":[1904],"recognize":[1906],"2\u2014figure":[1914,1938,2193,3299],"supplement":[1915,1939,2194,3300,3341,3399],"1).":[1916,2147,3342],"ran":[1918,2293],"purified":[1929,1946,2189,2433,2554,2869,2929],"6-His":[1930],"WT":[1931,2045,2079,2221,2297,2347,2448,2458,2472,2576,2635,2654,2666,2701,2724,2953,3136,3313,3660,3699,3730,3834,4035,4132,4161,4423],"2).":[1940],"Although":[1941],"equal":[1942],"concentrations":[1943],"were":[1948,2007,2027,2068,2125,2310,2324,2628,2698,2718,3003,3007,3066,3124,3180,3251,3350,3686,4358,4375],"loaded,":[1949],"poorly":[1954],"recognized":[1955,1992,3840],"likely":[1974,2270,3270],"similar":[1981],"not":[1990,2358,2397,2813,2830,3663,3839],"robustly":[1991,3687,3842],"antibody.":[1997,3847,4224,4236],"Next,":[1998],"after":[1999],"confirming":[2000],"expressed,":[2008],"assessed":[2010,2543],"shapes":[2012],"(\u2206NT":[2024],"BD)":[2026],"indistinguishable":[2028],"2C":[2033],"D),":[2035],"while":[2036,2361,2401,2748,2853],"resembled":[2044,2121,2653,2675],"Cells":[2047],"lacked":[2049],"(\u2206MM)":[2056],"last":[2059],"(\u220613\u201317)":[2067],"had":[2071],"slightly":[2072],"values":[2076],"population,":[2080],"it.":[2094],"Circular":[2095,2148,2429,2444,2468],"dichroism":[2096,2149,2430,2445,2469],"spectroscopy":[2097,2151],"reveals":[2098],"maintain":[2103],"\u03b2-strand":[2104,2247],"secondary":[2105,2158,2211,2248],"Considering":[2107],"result":[2109],"mutants,":[2123],"curious":[2126],"loss":[2128,2381,2412,2418,3017],"might":[2135],"destabilize":[2136],"formation":[2141,2828],"predicted":[2144],"(CD)":[2150],"folding":[2161],"Different":[2164],"structural":[2165],"elements":[2166],"characteristic":[2168,2240],"CD":[2169,2198,2218,2294,2333,2522],"spectra,":[2170],"consist":[2174],"negative":[2178,2241,2546,2867],"peaks":[2179],"216":[2181,2244],"nm":[2182,2204,2587,2606],"(Greenfield,":[2183],"2006).":[2184],"recombinantly":[2186],"6-his":[2190,2434],"2)":[2195],"collected":[2197,4394],"spectra":[2199,2219,2334,2344,2431,2446,2470,2523],"200":[2201],"260":[2203],"fixed":[2206],"temperatures":[2207],"measure":[2214,2583],"thermal":[2216],"stability.":[2217],"\u2206NT,":[2227,2363],"\u2206CT,":[2228,3315],"20\u00b0C":[2232,2342],"look":[2234],"very":[2235,2269],"similar;":[2236],"they":[2237,2323,3837],"peak":[2242],"nm,":[2245],"3A).":[2251],"data,":[2254],"combined":[2255],"1C,":[2263],"suggest":[2264,2737,2804,3785],"confirms":[2275],"neither":[2277],"fold":[2287],"properly.":[2288],"contrast,":[2290],"scans":[2295],"95\u00b0C":[2300,2350],"point":[2304,2485],"previously":[2308,2566,3050,3654],"shown":[2309,3051,3592,4108,4277],"unable":[2311],"able":[2312,3664,4484],"rescue":[2314],"null":[2317],"defect,":[2320],"nonfunctional":[2325],"2020)":[2329],"20\u00b0C.":[2331],"(I55A":[2338],"L110S)":[2340],"resemble":[2343],"melted":[2346,2479],"3B),":[2352],"folded.":[2359],"BD,":[2364,2406,3743],"I55A,":[2365],"L110S":[2367],"phenotype":[2374,3926],"mechanism":[2388],"differs.":[2389],"I55A":[2392],"L110S,":[2394,2490],"folded":[2398,3004],"nonfunctional,":[2400],"\u2206NT":[2404,2460,2622,2644,2676,2957,3740,3876,3886,3917,4126],"folded,":[2410],"causes":[2416],"3":[2424],"suggests":[2436,4454],"predominantly":[2441],"\u03b2-sheets.":[2442],"room":[2455,2475],"temperature":[2456,2476],"(20\u00b0C),":[2457],"(black),":[2459],"(pink),":[2463],"(cyan).":[2466],"(20\u00b0C,":[2477],"black),":[2478],"(95\u00b0C,":[2480],"dotted":[2481],"line),":[2482],"(I55A,":[2487],"red,":[2488],"burgundy).":[2491],"alone":[2495],"capable":[2497,3008],"polymerization:":[2499],"lateral":[2506,2858],"filaments":[2510,2581,2597,2624,2642,2657,2770],"Given":[2511,2996],"mutant":[2516,3247,3264,3273,3306,3376],"appeared":[2518],"stable":[2519],"displayed":[2521],"indicative":[2524],"beta":[2527],"structure,":[2529],"wondered":[2531],"phenotypes":[2536,3022],"correlated":[2537],"altered":[2539],"properties.":[2541],"stain":[2547],"transmission":[2549],"electron":[2550,2863],"microscopy":[2551,2864],"(TEM)":[2552,2865],"compared":[2560],"those":[2561,2721],"full-length":[2563],"protein.":[2564,3905],"As":[2565,3965,4013],"reported":[2567,3655],"(Holtrup":[2568],"Taylor":[2572],"2020),":[2575],"polymerizes":[2578],"into":[2579],"small":[2580],"average":[2585],"3.04":[2586],"(SD":[2588,2607],"0.79,":[2590],"18)":[2593],"width.":[2595],"wrap":[2598],"bundles":[2602,2626,2659,2696,2716,2764,2895,2908,2931],"21.34":[2605],"4.82,":[2609],"24)":[2612],"wide,":[2613],"lattice":[2616,2648,2827,2901],"structures":[2617,2649],"4A":[2619],"G).":[2621,2673,2711,2734],"homogenous,":[2630],"straighter,":[2631],"thinner":[2633],"appear":[2637],"2\u20134":[2641],"each.":[2643],"failed":[2645,2704,2727],"4B).":[2651],"forming":[2656],"width":[2664,2762,2927],"lattices":[2669,2707,2730],"4C":[2671],"4D":[2678],"G),":[2680],"13\u201317":[2685],"bundling":[2690],"properties":[2691],"\u2206NT.":[2693],"wider":[2699],"4E":[2709],"Lastly,":[2712],"straight":[2715],"narrower":[2719],"samples":[2725,4392],"4F":[2732],"N-":[2750],"regulate":[2756],"interpolymer":[2757],"number":[2768],"bundle,":[2774],"lattices.":[2782],"Taken":[2783],"2,":[2790],"pylori,":[2801],"polymerize":[2811],"does":[2829],"correlate":[2831],"4":[2837],"promote":[2857],"polymer":[2859],"interactions.":[2860,4067],"(A\u2013F)":[2861],"Transmission":[2862],"stained,":[2868],"500":[2874],"nm.":[2875],"White":[2876,2892],"micrograph":[2881],"enlarged":[2884],"right":[2889],"original.":[2891],"arrows":[2893,2899],"black":[2898],"structures,":[2902],"red":[2904],"arrow":[2905],"wrapping":[2909],"around":[2910],"other.":[2912],"(G)":[2921],"Box":[2922],"whiskers":[2924],"plot":[2925],"filaments.":[2933],"Median,":[2934],"min,":[2935],"max,":[2936],"25th":[2937],"percentile,":[2938],"75th":[2940],"percentile":[2941],"displayed.":[2943],"One-way":[2944],"ANOVA,":[2945],"Dunnett\u2019s":[2946],"multiple-comparison":[2947],"test.":[2948],"ns":[2949],"p>0.5,":[2950],"***p\u22640.001,":[2951],"****p\u22640.0001.":[2952],"24,":[2956,2972],"29,":[2960],"25,":[2964],"31,":[2968],"32.":[2976],"mediates":[2980,3792],"properly":[3005],"vitro,":[3012],"observed":[3024],"caused":[3038,3715],"disrupting":[3040],"proteins,":[3057],"experiments.":[3063,3642,4349],"Csd5-CcmA":[3064],"readily":[3067,3879],"blotting.":[3077],"Csd7-CcmA":[3079,3104,4062,4378],"reagent":[3088],"included":[3090],"weak":[3107],"transient":[3110],"Interactions":[3119],"never":[3125,3776],"background":[3139,3676],"(Blair,":[3140],"Blair":[3142],"probe":[3151,3429,3491,4184],"contributes":[3157],"locus,":[3192],"rdxA":[3196,3983,4105],"described":[3199],"earlier,":[3200],"csd5-2X-FLAG":[3203],"csd7-3X-FLAG":[3205],"loci.":[3209],"immunoprecipitated":[3211,3244,3648],"Csd5-FLAG":[3213,3338,3637],"Csd7-FLAG":[3215,3639,3724,4346,4397],"\u0251-FLAG":[3217,3469,3551,3631,4243],"agarose":[3218],"beads,":[3219],"then":[3220,4148],"still":[3240,3275],"co-purify.":[3241]},"counts_by_year":[],"updated_date":"2026-11-01T11:40:01.658920","created_date":"2022-10-01T00:00:00"}
