{"id":"https://openalex.org/W429260733","doi":null,"title":"Investigations on [FeFe]-hydrogenase active site biosynthesis","display_name":"Investigations on [FeFe]-hydrogenase active site biosynthesis","publication_year":2010,"publication_date":"2010-01-01","ids":{"openalex":"https://openalex.org/W429260733","mag":"429260733"},"language":"en","primary_location":{"id":"pmh:oai:scholarworks.montana.edu:1/1830","doi":null,"is_oa":true,"landing_page_url":"https://scholarworks.montana.edu/bitstreams/68348ff4-2ef5-438e-a2d8-a229424f2da3/download","pdf_url":"https://scholarworks.montana.edu/xmlui/handle/1/1830","source":{"id":"https://openalex.org/S4306402563","display_name":"Montana State University ScholarWorks (Montana State University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I23732399","host_organization_name":"Montana State University","host_organization_lineage":["https://openalex.org/I23732399"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Dissertation"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://scholarworks.montana.edu/xmlui/handle/1/1830","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5077229931","display_name":"Shawn E. McGlynn","orcid":"https://orcid.org/0000-0002-8199-7011","observed_orcids":["https://orcid.org/0000-0002-8199-7011"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Shawn E. McGlynn","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-8199-7011","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5077229931"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":"1","last_page":"121"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11626","display_name":"Metalloenzymes and iron-sulfur proteins","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11626","display_name":"Metalloenzymes and iron-sulfur proteins","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10284","display_name":"Anaerobic Digestion and Biogas Production","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2105","display_name":"Renewable Energy, Sustainability and the Environment"},"field":{"id":"https://openalex.org/fields/21","display_name":"Energy"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11747","display_name":"Metal-Catalyzed Oxygenation Mechanisms","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1604","display_name":"Inorganic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/fefe-hydrogenase","display_name":"[FeFe]-hydrogenase","score":0.996999979019165},{"id":"https://openalex.org/keywords/hydf","display_name":"HydF","score":0.925000011920929},{"id":"https://openalex.org/keywords/hydg","display_name":"HydG","score":0.972000002861023},{"id":"https://openalex.org/keywords/h-cluster","display_name":"H-cluster","score":0.7039999961853027},{"id":"https://openalex.org/keywords/radical-sam-enzymes","display_name":"radical SAM enzymes","score":0.7799999713897705},{"id":"https://openalex.org/keywords/methanogenic-archaea","display_name":"methanogenic archaea","score":0.9369999766349792},{"id":"https://openalex.org/keywords/tyrosine-oxidation","display_name":"tyrosine oxidation","score":0.44699999690055847},{"id":"https://openalex.org/keywords/escherichia-coli-expression","display_name":"Escherichia coli expression","score":0.925000011920929}],"concepts":[{"id":"https://openalex.org/C79115784","wikidata":"https://www.wikidata.org/wiki/Q424135","display_name":"Hydrogenase","level":3,"score":0.8925126791000366},{"id":"https://openalex.org/C553450214","wikidata":"https://www.wikidata.org/wiki/Q851162","display_name":"Biosynthesis","level":3,"score":0.5808917284011841},{"id":"https://openalex.org/C41183919","wikidata":"https://www.wikidata.org/wiki/Q423026","display_name":"Active site","level":3,"score":0.553610622882843},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.41123995184898376},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2474684715270996},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.22503286600112915}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:scholarworks.montana.edu:1/1830","doi":null,"is_oa":true,"landing_page_url":"https://scholarworks.montana.edu/bitstreams/68348ff4-2ef5-438e-a2d8-a229424f2da3/download","pdf_url":"https://scholarworks.montana.edu/xmlui/handle/1/1830","source":{"id":"https://openalex.org/S4306402563","display_name":"Montana State University ScholarWorks (Montana State University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I23732399","host_organization_name":"Montana State University","host_organization_lineage":["https://openalex.org/I23732399"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Dissertation"},{"id":"mag:429260733","doi":null,"is_oa":false,"landing_page_url":"https://scholarworks.montana.edu/xmlui/bitstream/1/1830/1/McGlynnS0810.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:scholarworks.montana.edu:1/1830","doi":null,"is_oa":true,"landing_page_url":"https://scholarworks.montana.edu/bitstreams/68348ff4-2ef5-438e-a2d8-a229424f2da3/download","pdf_url":"https://scholarworks.montana.edu/xmlui/handle/1/1830","source":{"id":"https://openalex.org/S4306402563","display_name":"Montana State University ScholarWorks (Montana State University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I23732399","host_organization_name":"Montana State University","host_organization_lineage":["https://openalex.org/I23732399"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Dissertation"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W429260733.pdf","grobid_xml":"https://content.openalex.org/works/W429260733.grobid-xml"},"referenced_works_count":121,"referenced_works":["https://openalex.org/W10180145","https://openalex.org/W132604699","https://openalex.org/W585914646","https://openalex.org/W1482410919","https://openalex.org/W1538196126","https://openalex.org/W1539798739","https://openalex.org/W1553730591","https://openalex.org/W1569088652","https://openalex.org/W1630260213","https://openalex.org/W1711482514","https://openalex.org/W1865947077","https://openalex.org/W1882597211","https://openalex.org/W1902924213","https://openalex.org/W1957507493","https://openalex.org/W1963515928","https://openalex.org/W1964732846","https://openalex.org/W1966488638","https://openalex.org/W1973861730","https://openalex.org/W1978143120","https://openalex.org/W1979318377","https://openalex.org/W1982876627","https://openalex.org/W1983613885","https://openalex.org/W1983730749","https://openalex.org/W1983783030","https://openalex.org/W1983925681","https://openalex.org/W1984677796","https://openalex.org/W1984764939","https://openalex.org/W1986556298","https://openalex.org/W1989362657","https://openalex.org/W1990915279","https://openalex.org/W1993406712","https://openalex.org/W1993719422","https://openalex.org/W1994126548","https://openalex.org/W1995925875","https://openalex.org/W1996711495","https://openalex.org/W2001209724","https://openalex.org/W2004505321","https://openalex.org/W2005324474","https://openalex.org/W2006060111","https://openalex.org/W2009246651","https://openalex.org/W2022682403","https://openalex.org/W2027803750","https://openalex.org/W2027861057","https://openalex.org/W2028465433","https://openalex.org/W2030539878","https://openalex.org/W2033367616","https://openalex.org/W2035285014","https://openalex.org/W2036212369","https://openalex.org/W2036932625","https://openalex.org/W2039892768","https://openalex.org/W2041438836","https://openalex.org/W2044291290","https://openalex.org/W2046449734","https://openalex.org/W2059497349","https://openalex.org/W2060801760","https://openalex.org/W2060991757","https://openalex.org/W2063774369","https://openalex.org/W2066670002","https://openalex.org/W2068445334","https://openalex.org/W2069140334","https://openalex.org/W2069255722","https://openalex.org/W2069649399","https://openalex.org/W2073323000","https://openalex.org/W2077167882","https://openalex.org/W2077390076","https://openalex.org/W2084182165","https://openalex.org/W2084744164","https://openalex.org/W2085822891","https://openalex.org/W2087040208","https://openalex.org/W2087052747","https://openalex.org/W2088557547","https://openalex.org/W2090079874","https://openalex.org/W2092553727","https://openalex.org/W2092638302","https://openalex.org/W2093173456","https://openalex.org/W2095217602","https://openalex.org/W2096692378","https://openalex.org/W2099723319","https://openalex.org/W2103546861","https://openalex.org/W2104796556","https://openalex.org/W2106260539","https://openalex.org/W2108684777","https://openalex.org/W2111854009","https://openalex.org/W2112378657","https://openalex.org/W2114484640","https://openalex.org/W2116472408","https://openalex.org/W2116626109","https://openalex.org/W2122594388","https://openalex.org/W2123894662","https://openalex.org/W2124924735","https://openalex.org/W2128474182","https://openalex.org/W2128635872","https://openalex.org/W2129921499","https://openalex.org/W2136347342","https://openalex.org/W2139515062","https://openalex.org/W2141066579","https://openalex.org/W2141449959","https://openalex.org/W2141756313","https://openalex.org/W2143766385","https://openalex.org/W2145926526","https://openalex.org/W2146058063","https://openalex.org/W2146348320","https://openalex.org/W2147608975","https://openalex.org/W2150710339","https://openalex.org/W2151639345","https://openalex.org/W2153415066","https://openalex.org/W2153777986","https://openalex.org/W2156689371","https://openalex.org/W2160084832","https://openalex.org/W2160760745","https://openalex.org/W2161444534","https://openalex.org/W2162759883","https://openalex.org/W2162956402","https://openalex.org/W2164609484","https://openalex.org/W2164633611","https://openalex.org/W2166554692","https://openalex.org/W2169783079","https://openalex.org/W2171086890","https://openalex.org/W2171852113","https://openalex.org/W2172095207","https://openalex.org/W2798889656"],"related_works":["https://openalex.org/W4400129519","https://openalex.org/W2315821709","https://openalex.org/W1593166445","https://openalex.org/W2461093443","https://openalex.org/W3171663240","https://openalex.org/W2328445276","https://openalex.org/W2978497242","https://openalex.org/W2211081036","https://openalex.org/W2936381024","https://openalex.org/W1968797572"],"abstract_inverted_index":{"Hydrogenase":[0],"enzymes,":[1],"which":[2,107],"catalyze":[3],"the":[4,30,55,62,72,86,89,98,109,125,154,163,190,193,216,219,225],"reversible":[5],"oxidation":[6],"of":[7,32,34,45,48,61,88,111,129,131,165,175,192,218],"molecular":[8],"hydrogen,":[9],"occupy":[10],"important":[11],"roles":[12],"as":[13,70,84,96,142],"catalysts":[14],"in":[15,76,102,183,189,196],"microbial":[16],"energy":[17],"transfer":[18],"and":[19,27,57,81,124,153,167,211],"conservation.":[20],"This":[21],"seemingly":[22],"simple":[23],"reaction":[24,51],"between":[25],"protons":[26],"electrons":[28],"necessitates":[29],"utilization":[31],"some":[33],"nature's":[35],"most":[36],"complicated":[37],"organo-metallic":[38,209],"cofactors.":[39],"Remarkably,":[40],"two":[41,130],"evolutionarily":[42],"independent":[43],"types":[44],"enzymes":[46,67],"capable":[47],"catalyzing":[49],"this":[50,103],"exist":[52],"-":[53],"termed":[54],"[NiFe]":[56],"[FeFe]-hydrogenases.":[58],"The":[59,134],"biosynthesis":[60,87,191],"cofactors":[63],"harbored":[64],"by":[65],"these":[66,132,199],"poses":[68],"questions":[69],"to":[71,85,97,139,159,202,206,224,228],"assembly":[73],"pathways":[74],"involved":[75,101],"constructing":[77],"hydrogen":[78],"competent":[79],"catalysts,":[80],"herein":[82],"research":[83],"[FeFe]-hydrogenase":[90,149],"active":[91,150],"site":[92,151],"is":[93,137,157,181],"presented.":[94],"Data":[95],"protein":[99,120,136,156],"components":[100],"process":[104],"are":[105],"presented":[106],"include":[108],"development":[110],"an":[112,143],"E.coli":[113],"based":[114],"expression":[115],"system":[116],"for":[117,148,162,215],"hydrogenase":[118],"maturation":[119],"factors,":[121],"their":[122],"isolation,":[123],"first":[126],"functional":[127],"assignment":[128],"proteins.":[133],"HydF":[135],"shown":[138],"be":[140,160],"operative":[141],"H-cluster":[144],"intermediate":[145],"bearing":[146],"scaffold":[147],"assembly,":[152],"HydG":[155],"demonstrated":[158],"responsible":[161],"formation":[164],"cyanide":[166],"carbon":[168],"monoxide":[169],"from":[170],"tyrosine.":[171],"In":[172],"addition,":[173],"observations":[174,200],"a":[176,213],"novel":[177],"radical":[178],"SAM":[179],"enzyme":[180],"reported":[182],"conjunction":[184],"with":[185],"its":[186],"putative":[187],"involvement":[188],"Hmd-hydrogenase":[194],"found":[195],"methanogens.":[197],"Together,":[198],"contribute":[201],"understanding":[203],"biology's":[204],"ability":[205,227],"construct":[207],"complex":[208,230],"cofactors,":[210],"lay":[212],"foundation":[214],"consideration":[217],"evolutionary":[220],"events":[221],"that":[222],"led":[223],"biological":[226],"assemble":[229],"metallocofactors.":[231]},"counts_by_year":[],"updated_date":"2026-09-11T09:22:31.653822","created_date":"2016-06-24T00:00:00"}
